Nicole Guiso - Academia.edu (original) (raw)

Papers by Nicole Guiso

The enzyme-linked immunosorbent assay is widely employed for the serological diagnosis of pertuss... more The enzyme-linked immunosorbent assay is widely employed for the serological diagnosis of pertussis. It is generally concluded that a significant increase in specific immunoglobulin G (IgG) or IgA against the pertussis toxin (PT) or against filamentous hemagglutinin (FHA) in paired sera correlates with Bordetella pertussis infection. However, this type of diagnosis of pertussis has mainly been applied to unvaccinated children,

Bordetella pertussis induces in vitro apoptosis of murine alveolar macrophages by a mechanism tha... more Bordetella pertussis induces in vitro apoptosis of murine alveolar macrophages by a mechanism that is dependent on expression of bacterial adenylate cyclase-hemolysin. Using a murine respiratory model, we found in this study that intranasal infection with a parental B. pertussis strain, but not with an isogenic variant deficient in the expression of all toxins and adhesins, induced a marked neutrophil

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, Jan 18, 2015

Bordetella bronchiseptica is an opportunistic bacteria infecting the respiratory tract of patient... more Bordetella bronchiseptica is an opportunistic bacteria infecting the respiratory tract of patients with cystic fibrosis. We present a case of B. bronchiseptica chronic pulmonary infection and documentation of some phenotypic attributes of the clinical isolates allowing the microorganism to induce progressive respiratory degradation and chronic sputum colonization. We recommend implementing adequate treatment aiming eradication from the first isolation of this bacterium. We advise for practices that minimize opportunities for zoonotic transmission of B. bronchiseptica from family pets.

Expert review of vaccines, 2014

The Bordetella genus comprises nine species of which Bordetella pertussis and B. parapertussis ar... more The Bordetella genus comprises nine species of which Bordetella pertussis and B. parapertussis are isolated from humans and are the most studied Bordetella species since they cause whooping cough. They both originate from B. bronchiseptica, which infects several mammals and immune compromised humans, but the intensive use of pertussis vaccines induced changes in B. pertussis and B. parapertussis populations. B. petrii and B. holmesii are other species of unknown reservoir and transmission pattern that have been described in humans. It is still unknown whether these species are pathogens for humans or only opportunistic bacteria but biological diagnosis has confirmed the presence of B. holmesii in human respiratory samples while B. petrii and the four other species have little implications for public health.

Genome announcements, 2015

Here, we present the genome sequence of Corynebacterium ulcerans strain FRC11. The genome include... more Here, we present the genome sequence of Corynebacterium ulcerans strain FRC11. The genome includes one circular chromosome of 2,442,826 bp (53.35% G+C content), and 2,210 genes were predicted, 2,146 of which are putative protein-coding genes, with 12 rRNAs and 51 tRNAs; 1 pseudogene was also identified.

Vaccine, 2007

Humoral and cell-mediated immune responses (CMI) were evaluated in subjects 3 and 6 years after p... more Humoral and cell-mediated immune responses (CMI) were evaluated in subjects 3 and 6 years after primary and booster vaccination with either three-component acellular (Pa) or whole-cell (Pw) vaccines. Low anti-pertussis toxin (PT) antibody levels confirmed the absence of pertussis disease, consistent with ongoing protection. Anti-pertactin (PRN) antibodies, remained at higher levels in Pa-vaccinated subjects. At year 6, CMI responses continued to be present and were higher in Pa-vaccinated than Pw-vaccinated subjects. Long-term protection with Pa vaccines can be expected to be at least as good as that provided by efficacious Pw vaccines.

Lancet, 1999

Older children still contribute to the transmission of pertussis to infants in French regions wit... more Older children still contribute to the transmission of pertussis to infants in French regions with low booster coverage at 2 years of age. Booster vaccination seems crucial for protection against symptomatic pertussis in childhood.

Microbes and Infection, 2006

Bordetella pertussis, a Gram-negative β-proteobacterium, is the agent of whooping cough in humans... more Bordetella pertussis, a Gram-negative β-proteobacterium, is the agent of whooping cough in humans. Whooping cough remains a public health problem worldwide, despite well-implemented infant/child vaccination programs. It continues to be endemic and is observed cyclically in vaccinated populations. Classical molecular subtyping methods indicate that genome diversity among B. pertussis isolates is limited. Although the whole bacterial genome has been studied

Journal of Clinical Microbiology, 2005

Microbes and Infection, 2005

The increasing incidence of pertussis in a number of countries, despite good vaccination coverage... more The increasing incidence of pertussis in a number of countries, despite good vaccination coverage, is a cause for concern. We used pulsed-field gel electrophoresis (PFGE) typing to examine the genetic diversity of 101 clinical isolates of Bordetella pertussis, recovered during 1999–2001, and circulating in five different European countries to evaluate temporal and geographical distribution. This DNA fingerprinting approach seems to

The adenylate cyclase toxin (CyaA) of Bordetella pertussis is a major virulence factor required f... more The adenylate cyclase toxin (CyaA) of Bordetella pertussis is a major virulence factor required for the early phases of lung colonization. It can invade eukaryotic cells where, upon activation by endogenous calmodulin, it catalyzes the formation of unregulated cAMP levels. CyaA intoxi- cation leads to evident toxic effects on macrophages and neutrophils. Here, we demonstrate that CyaA uses the a

We used pulsed-field gel electrophoresis analysis and genotyping to compare clinical isolates of ... more We used pulsed-field gel electrophoresis analysis and genotyping to compare clinical isolates of Bordetella per- tussis recovered since the early 1990s in Finland and France, 2 countries with similar histories of long-term mass vaccination with whole-cell pertussis vaccines. Isolates from both countries were similar genetically but varied temporally.

Despite the widespread use of pertussis vaccines during the last decades, pertussis has remained ... more Despite the widespread use of pertussis vaccines during the last decades, pertussis has remained an endemic disease with frequent epidemic outbreaks. Currently two types of vaccines are used: whole-cell vaccines (WCVs) and recently developed acellular vaccines (ACVs). The long-term aim of our studies is to assess the effect of different vaccination policies on the population structure of Bordetella pertussis and

Infection and Immunity, 2000

In gram-negative bacteria, high-affinity iron uptake requires the TonB/ExbB/ExbD envelope complex... more In gram-negative bacteria, high-affinity iron uptake requires the TonB/ExbB/ExbD envelope complex to release iron chelates from their specific outer membrane receptors into the periplasm. Based on sequence similarities, the Bordetella pertussis tonB exbB exbD locus was identified on a cloned DNA fragment. The tight organization of the three genes suggests that they are cotranscribed. A putative Fur-binding sequence located upstream

We compared Bordetella pertussis isolates collected in France over the last 10 years, the vaccine... more We compared Bordetella pertussis isolates collected in France over the last 10 years, the vaccine strains used for more than 30 years, and isolates collected before the introduction of generalized vaccination. The analysis included serotyping, pulsed-field gel electrophoresis of chromosomal DNA after digestion with XbaI and SpeI, and sequencing of the pt S1 gene, encoding the S1 subunit of pertussis

Médecine et Maladies Infectieuses, 2011

The authors had for aim to analyze pertussis epidemiology in Tunisia by studying nasopharyngeal s... more The authors had for aim to analyze pertussis epidemiology in Tunisia by studying nasopharyngeal specimens of infants hospitalized in Tunis. Between march 2007 and march 2008, clinical nasopharyngeal samples were collected from infants with a suspected diagnosis of whooping cough, pertussoid cough, or pertussis-like syndrome, admitted at the Tunis children's hospital. The laboratory diagnostic criteria were culture isolation of Bordetella species on Bordet-Gengou medium and real-time PCR. Fifty-nine percent of the 74 investigated children with suspected pertussis were less than two months of age. The diagnosis of pertussis was proved positive by real-time PCR for 41%. Culture was negative in all cases. Whooping cough is still prevalent in Tunisia despite an important vaccination coverage. Real-time PCR is an invaluable tool for the rapid diagnosis of pertussis, however culture must also be associated.

Journal of Immunological Methods, 2014

A Fur titration assay was used to isolate DNA fragments bearing putative Fur binding sites (FBS) ... more A Fur titration assay was used to isolate DNA fragments bearing putative Fur binding sites (FBS) from a partial Bordetella bronchiseptica genomic DNA library. A recombinant plasmid bearing a 3.5-kb DNA insert was further studied. Successive deletions in the cloned fragment enabled us to map a putative FBS at about 2 kb from one end. Sequence analysis revealed the presence