Nivaldo Antonio Parizotto - Academia.edu (original) (raw)
Papers by Nivaldo Antonio Parizotto
Fisioterapia Brasil, 2016
O objetivo desta revisão é complementar os dados revisados na parte 1 onde foram discutidos princ... more O objetivo desta revisão é complementar os dados revisados na parte 1 onde foram discutidos princípios e generalidades, disponibilizar ao leitor referencial teórico para conhecer a interação do laser de baixa intensidade com os diferentes tipos de tecidos e analisar as distintas respostas. Este material constitui um acervo atualizado onde se especificam os diversos parâmetros aplicados e a técnica utilizados em cada trabalho, o qual vai servir para que os profissionais interessadas nesta área direcionem melhor suas pesquisas e determinem com maior precisão as doses para o tratamento de seus pacientes ao contar com diferentes alternativas.Palavras-chave: laser de baixa intensidade, bioefeitos, fotoreatividade tecidual.
Hansenologia Internationalis, Nov 29, 2000
A hanseníase apresenta, como incapacidade decorrente muito comum, a ulceração plantar. Tendo em v... more A hanseníase apresenta, como incapacidade decorrente muito comum, a ulceração plantar. Tendo em vista a necessidade de implantação de programas de ação de controle da doença e melhora da qualidade de vida dos portadores desta nos serviços básicos de saúde, este projeto viabilizou, comparativamente a afetados pela mesma lesão, porém de outras etiologias, a utilização da laserterapia de baixa intensidade para cicatrização das úlceras hansênicas de membros inferiores. Na sua adoção, uma vez claramente definidos população de referência, bem como respectivos critérios de inclusão e exclusão, a normalização ética vigente no país prevista foi rigorosamente cumprida. Promoveram-se procedimentos adequados para padronização de condutas de mensuração, avaliação, aplicação e acompanhamento da 1 Projeto parcialmente financiado pela Fundação Paulista Contra Hanseníase (FPCH) e apoiado por bolsa da Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES);
The FASEB Journal
Recent research evidences substantial morphological and physiological differences between cells m... more Recent research evidences substantial morphological and physiological differences between cells maintained in vitro in two‐dimensional (2D) and three‐dimensional (3D) culture conditions. The current consensus is that 3D culture is better because it precisely mimics the cellular microenvironment in vivo. The biomodulatory effect of low‐intensity laser radiation (LILR) on cells in 2D cultures is already well established. On the other hand, the effect of this radiation on cells in 3D cultures has not yet been widely investigated. The aim of this work was to analyze the biomodulatory effect of LILR, at the wavelength (ƛ) of 685 nm, on the viability of osteogenic precursor cells (OPC) cultured as multicellular spheroids (MS). Agarose molds containning wells (300 μm diameter) were used for MS formation using the non‐adherent surface culture concept. A cell count (2×105) of the OPC lineage (MC3T3) was seeded in each mold and maintained under ideal growth conditions (Dulbecco's Modified...
The FASEB Journal
The aim of this study was to analyze the influence of low intensity laser irradiation at waveleng... more The aim of this study was to analyze the influence of low intensity laser irradiation at wavelengths (λ) of 670, 685 and 830 nm on the mitochondrial activity and gene expression profile of myogenic precursor cells (MPCs) in vitro. Primary rat MPCs were sown in multiwell dishes of 96 and 6 wells. Six experimental groups were set up: a non‐irradiated control group and five groups ranged by differing doses of radiation (0.3; 0.6; 0.9; 1.2 and 1.5 J/cm2). After 24 hours of irradiation, the mitochondrial activity was quantified using the colorimetric technique MTT‐formazan and established in terms of percentage in relation to the control. The gene expression profile was established through the quantitative analysis of the related isoforms IIa and IIx of the myosin heavy chain (MHC) by RT‐PCR five days after irradiation. The results showed that irradiation at λ of 670, 685 and 830 nm promoted respective increase of 56.8, 70.6 and 84.8 % on mitochondrial activity. These values were achieve...
Lasers in Medical Science
Cureus, May 22, 2023
Background Primary dysmenorrhea (PD) refers to the occurrence of painful menstrual cramps without... more Background Primary dysmenorrhea (PD) refers to the occurrence of painful menstrual cramps without pathological involvement of the pelvic organs, with considerable morbidity and high prevalence among females of reproductive age. Objective The objective of this study is to present and test the efficacy of an innovative method of interactive transcutaneous electrical nerve stimulation (iTENS) for PD. Methods and materials This study is a single-blind controlled clinical trial. This was conducted at the outpatient clinic of the faculty of physical therapy. Females with PD (n=124) were divided into the treated group (transcutaneous electrical nerve stimulation {TENS} group {TG}, n=62) and the placebo group (PG, n=62). A single session of either iTENS or placebo intervention was used for 35 minutes. Pain, the duration of analgesia, and the use of pain medication were assessed before and after the intervention. Data from before and after the treatment were compared between groups (Student's t-test). The level of significance was set at 5%. Results A significant decrease in pain (p<0.001) was observed after the intervention for the TG, with a more longlasting analgesia (p<0.001) and decreased need for pain medication (p<0.001). Conclusions The proposed method of transcutaneous electrical nerve stimulation (TENS) application showed positive results for pain management on females with PD, with no reported adverse effects. The new proposed TENS application takes into account the preferences of the patient regarding positioning and the number of channels needed to cause analgesia. This application was able to promote almost complete analgesia in females with primary dysmenorrhea, and the analgesia persisted for more than one menstrual cycle.
Journal of Photochemistry and Photobiology B-biology, May 1, 2019
Follicular cystic ovary disease is a common reproductive disorder in women and females of domesti... more Follicular cystic ovary disease is a common reproductive disorder in women and females of domestic animals, characterized by anovulation and the persistence of follicle is a common cause of reproductive failure in mammalian. Polycystic ovary syndrome (PCOS) is characterized by hyperandrogenism (HA), chronic anovulation and polycystic ovaries, and it is a common reproductive endocrine disease with clinical manifestations including hirsutism, acne, infertility and obesity that can affect 5-20% of women in their reproductive age. Photobiomodulation (PBM) has been investigated and used in clinical practice, related to biomodulatory influences on cellular functions in animals and humans, both in vivo and in vitro. In this study, we include endocrine and reproductive features in a rat model for PCOS and the effects of PBM on ovarian activities. Forty-five adult female Wistar rats PCOS-induced by a single dose of the estradiol valerate (EV) were used in the study. After the EV injection for PCO induction, rats were divided into 9 groups (n = 5/group) named C30, C45 and C60 (Control group), S30, S45 and S60 (PCO group) and L30, L45 and L60 (PCO/Laser group). The rats were irradiated with laser 3 times/week. The results shown that EV PCO-induced rats had increased body mass, reduced ovary mass, and reduced GSI. The plasma levels of P4 and T were increased, and the LH plasma level was decreased by PBM stimulation. The number of ovarian follicles and corpus luteum were increased, and the number of ovarian cysts was decreased by PBM stimulation. Thus, reproductive and endocrine characteristics were modulated by PBM.
Hansenologia Internationalis, Nov 29, 2000
A hanseníase apresenta, como incapacidade decorrente muito comum, a ulceração plantar. Tendo em v... more A hanseníase apresenta, como incapacidade decorrente muito comum, a ulceração plantar. Tendo em vista a necessidade de implantação de programas de ação de controle da doença e melhora da qualidade de vida dos portadores desta nos serviços básicos de saúde, este projeto viabilizou, comparativamente a afetados pela mesma lesão, porém de outras etiologias, a utilização da laserterapia de baixa intensidade para cicatrização das úlceras hansênicas de membros inferiores. Na sua adoção, uma vez claramente definidos população de referência, bem como respectivos critérios de inclusão e exclusão, a normalização ética vigente no país prevista foi rigorosamente cumprida. Promoveram-se procedimentos adequados para padronização de condutas de mensuração, avaliação, aplicação e acompanhamento da 1 Projeto parcialmente financiado pela Fundação Paulista Contra Hanseníase (FPCH) e apoiado por bolsa da Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES);
The FASEB Journal
Recent research evidences substantial morphological and physiological differences between cells m... more Recent research evidences substantial morphological and physiological differences between cells maintained in vitro in two‐dimensional (2D) and three‐dimensional (3D) culture conditions. The current consensus is that 3D culture is better because it precisely mimics the cellular microenvironment in vivo. The biomodulatory effect of low‐intensity laser radiation (LILR) on cells in 2D cultures is already well established. On the other hand, the effect of this radiation on cells in 3D cultures has not yet been widely investigated. The aim of this work was to analyze the biomodulatory effect of LILR, at the wavelength (ƛ) of 685 nm, on the viability of osteogenic precursor cells (OPC) cultured as multicellular spheroids (MS). Agarose molds containning wells (300 μm diameter) were used for MS formation using the non‐adherent surface culture concept. A cell count (2×105) of the OPC lineage (MC3T3) was seeded in each mold and maintained under ideal growth conditions (Dulbecco's Modified...
The FASEB Journal
The aim of this study was to analyze the influence of low intensity laser irradiation at waveleng... more The aim of this study was to analyze the influence of low intensity laser irradiation at wavelengths (λ) of 670, 685 and 830 nm on the mitochondrial activity and gene expression profile of myogenic precursor cells (MPCs) in vitro. Primary rat MPCs were sown in multiwell dishes of 96 and 6 wells. Six experimental groups were set up: a non‐irradiated control group and five groups ranged by differing doses of radiation (0.3; 0.6; 0.9; 1.2 and 1.5 J/cm2). After 24 hours of irradiation, the mitochondrial activity was quantified using the colorimetric technique MTT‐formazan and established in terms of percentage in relation to the control. The gene expression profile was established through the quantitative analysis of the related isoforms IIa and IIx of the myosin heavy chain (MHC) by RT‐PCR five days after irradiation. The results showed that irradiation at λ of 670, 685 and 830 nm promoted respective increase of 56.8, 70.6 and 84.8 % on mitochondrial activity. These values were achieve...
Este trabalho apresenta uma serie de resultados referentes a utilizacao da Microscopia de Forca A... more Este trabalho apresenta uma serie de resultados referentes a utilizacao da Microscopia de Forca Atomica (MFA) em aplicacoes na observacao de materiais biologicos, comprovando sua eficacia como importante metodo para investigacao de variados fenomenos, A estrutura molecular do colageno do tendao de rato foi utilizado como modelo de reparacao teciduaL Utilizou-se laser de ReNe (6 mW) como recurso no intuito de promover ou facilitar a cicatrizacao e se analisou por meio da MFA, Microscopia Eletronica de Varredura (MEV) e Espectroscopia de Infravermelho por Transformada de Fourier (FT-IR), estes ultimos com objetivos de dar um panorama de menor ampliacao do tecido em reparacao e avaliar os aspectos das possiveis modificacoes bioquimicas na estrutura das moleculas de colageno, respectivamente. A MEV mostrou evidencias de melhor organizacao na reparacao do tendao nos animais tratados com o laser de ReNe, acelerando-a em relacao aos animais controle, nao tratados. Os animais tratados pelo laser com diferentes doses, mostrou sinais de aceleracao do processo na dependencia da dose. As doses que foram mais eficientes foram 5 J/cm2, 50 J/cm2e 0,5 J/cm2 considerados em ordem decrescente. Tais resultados foram corroborados com a MFA, repetindo as caracteristicas de auto enrolamento, mesmo quando observado em resolucoes maiores. Nao houve modificacoes dignas de nota quando comparados os resultados obtidos com FT-IR. Provavelmente houve alguma falha na preparacao das amostra para este objetivo, uma vez que as amostras eram as mesmas preparadas para analises no MFA... Observacao: O resumo, na integra, podera ser visualizado no texto completo da tese digital Abstract
Lasers in Medical Science
Journal of Photochemistry and Photobiology B-biology, Mar 1, 2020
Cureus, May 22, 2023
Background Primary dysmenorrhea (PD) refers to the occurrence of painful menstrual cramps without... more Background Primary dysmenorrhea (PD) refers to the occurrence of painful menstrual cramps without pathological involvement of the pelvic organs, with considerable morbidity and high prevalence among females of reproductive age. Objective The objective of this study is to present and test the efficacy of an innovative method of interactive transcutaneous electrical nerve stimulation (iTENS) for PD. Methods and materials This study is a single-blind controlled clinical trial. This was conducted at the outpatient clinic of the faculty of physical therapy. Females with PD (n=124) were divided into the treated group (transcutaneous electrical nerve stimulation {TENS} group {TG}, n=62) and the placebo group (PG, n=62). A single session of either iTENS or placebo intervention was used for 35 minutes. Pain, the duration of analgesia, and the use of pain medication were assessed before and after the intervention. Data from before and after the treatment were compared between groups (Student's t-test). The level of significance was set at 5%. Results A significant decrease in pain (p<0.001) was observed after the intervention for the TG, with a more longlasting analgesia (p<0.001) and decreased need for pain medication (p<0.001). Conclusions The proposed method of transcutaneous electrical nerve stimulation (TENS) application showed positive results for pain management on females with PD, with no reported adverse effects. The new proposed TENS application takes into account the preferences of the patient regarding positioning and the number of channels needed to cause analgesia. This application was able to promote almost complete analgesia in females with primary dysmenorrhea, and the analgesia persisted for more than one menstrual cycle.
Fisioterapia Brasil, Dec 8, 2016
Resumo O objetivo desta revisão é complementar os dados revisados na parte 1 onde foram discutido... more Resumo O objetivo desta revisão é complementar os dados revisados na parte 1 onde foram discutidos princípios e generalidades, disponibilizar ao leitor referencial teórico para conhecer a interação do laser de baixa intensidade com os diferentes tipos de tecidos e analisar as distintas respostas. Este material constitui um acervo atualizado onde se especificam os diversos parâmetros aplicados e a técnica utilizados em cada trabalho, o qual vai servir para que os profissionais interessadas nesta área direcionem melhor suas pesquisas e determinem com maior precisão as doses para o tratamento de seus pacientes ao contar com diferentes alternativas.
Fisioterapia Brasil, Dec 8, 2016
O estresse faz-se presente em todas as reações do organismo desencadeadas por agressões de ordem ... more O estresse faz-se presente em todas as reações do organismo desencadeadas por agressões de ordem física, psíquica ou infecciosa. Situações de grande tensão pode provocar o estresse patológico, facilitando ou agravando distúrbios transitórios ou doenças graves. Verificaram-se os efeitos do Watsu em dezoito pacientes sob experimentação com características de estresse, submetendo-os aos procedimentos descritos pelos autores para a referida técnica hidroterápica. As diversas posições recomendadas pela técnica associadas aos benefícios da água aquecida mostrou promover momentos de profundo relaxamento. Mais de 50% dos participantes já havia realizado tratamento fisioterápico clássico com resultados insatisfatórios. Com o Watsu®, além dos pacientes relatarem melhoras das algias musculares, acrescentaram que houve um aumento no rendimento do trabalho, maior disposição para atividades da vida diária, mais flexibilidade e ponderação para resolver situações conflitantes, enfim uma melhora na homeostase. Pode-se concluir que é vantajosa a associação da hidroterapia com água hipertérmica e o uso de exercícios e alongamentos pela técnica de Watsu para controle do estresse.
Journal of Photochemistry and Photobiology B-biology, Dec 1, 2019
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
PubMed, May 14, 2010
Background: Ultrasound (US) is a widely used and studied resource for physical therapy treatments... more Background: Ultrasound (US) is a widely used and studied resource for physical therapy treatments. Given the scarcity of studies on the effects of US on healthy tissue, many physical therapy professionals make unfounded decisions regarding its methods and parameters of application. Objectives: The possible histological and morphometric changes in the healthy tissue of Wistar rats in vivo irradiated with different intensities of US were evaluated. Methods: Thirty Wistar rats, randomly distributed among five groups of six animals each, were treated on the right side of the dorsal region, over an area of 4 cm(2). The left side served as a control. The treatment was applied over a four-day period, with two mins. of daily irradiation. The output intensity was checked using a precision dosimeter before the applications. Histological and morphometric analyses were performed using the Image Tool software. Results: There were slight inflammatory infiltration and thinning of the dermis fibers, particularly in the groups irradiated with 1.5 and 2 W/cm(2). There was also thickening of the epidermis in the samples from the irradiated animals. To evaluate the quantitative results, the statistical analyses consisted of one-way ANOVAs with the post-hoc Tukey tests. There were significant differences in epidermis thicknesses between the control group and the groups irradiated with 1.0, 1.5 and 2.0 W/cm(2). Conclusions: Higher doses of US produced changes in the epidermis and dermis, i.e. increased thickness and collagen fiber thinning and proliferation, respectively. These results serve as a warning of the possible implications of therapeutic ultrasound use in esthetics.
Pain Practice, Apr 1, 2020
There is a wide range of animal models available today for studying chronic pain associated with ... more There is a wide range of animal models available today for studying chronic pain associated with a variety of etiologies and an extensive list of clinical manifestations of peripheral neuropathies. Photobiomodulation is a new tool for the treatment of pain in a convenient, non-invasive way. The aim of this work is to elucidate the effects of infrared light emitting diodes (LEDs) on behavioral responses to nociceptive stimuli in chronic pain models. Forty-eight Swiss male mice weighing 25-35 g were used. Two chronic pain models, ischemia-reperfusion (IR) and spared spinal nerve injury (SNI), were performed and then treated with infrared LED irradiation (390 mW, 890 nm, 17.3 mW/cm², 20.8 J/cm², for 20 min). The behavioral tests used were a mechanical hypersensitivity test (von Frey test) and a cold allodynia test (acetone test). The results showed that, in the ischemia-reperfusion model, the infrared LED had a significant effect on mechanical stimulation and cold allodynia on every day of treatment. In the spared nerve injury model, an analgesic effect was observed on every treatment day (when started on the 3 rd and 7 th days after the surgery). In both models, the effect was abolished when the treatment was interrupted. These findings suggest that photobiomodulation therapy may be a useful adjunct treatment for chronic pain.
Fisioterapia Brasil, 2016
O objetivo desta revisão é complementar os dados revisados na parte 1 onde foram discutidos princ... more O objetivo desta revisão é complementar os dados revisados na parte 1 onde foram discutidos princípios e generalidades, disponibilizar ao leitor referencial teórico para conhecer a interação do laser de baixa intensidade com os diferentes tipos de tecidos e analisar as distintas respostas. Este material constitui um acervo atualizado onde se especificam os diversos parâmetros aplicados e a técnica utilizados em cada trabalho, o qual vai servir para que os profissionais interessadas nesta área direcionem melhor suas pesquisas e determinem com maior precisão as doses para o tratamento de seus pacientes ao contar com diferentes alternativas.Palavras-chave: laser de baixa intensidade, bioefeitos, fotoreatividade tecidual.
Hansenologia Internationalis, Nov 29, 2000
A hanseníase apresenta, como incapacidade decorrente muito comum, a ulceração plantar. Tendo em v... more A hanseníase apresenta, como incapacidade decorrente muito comum, a ulceração plantar. Tendo em vista a necessidade de implantação de programas de ação de controle da doença e melhora da qualidade de vida dos portadores desta nos serviços básicos de saúde, este projeto viabilizou, comparativamente a afetados pela mesma lesão, porém de outras etiologias, a utilização da laserterapia de baixa intensidade para cicatrização das úlceras hansênicas de membros inferiores. Na sua adoção, uma vez claramente definidos população de referência, bem como respectivos critérios de inclusão e exclusão, a normalização ética vigente no país prevista foi rigorosamente cumprida. Promoveram-se procedimentos adequados para padronização de condutas de mensuração, avaliação, aplicação e acompanhamento da 1 Projeto parcialmente financiado pela Fundação Paulista Contra Hanseníase (FPCH) e apoiado por bolsa da Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES);
The FASEB Journal
Recent research evidences substantial morphological and physiological differences between cells m... more Recent research evidences substantial morphological and physiological differences between cells maintained in vitro in two‐dimensional (2D) and three‐dimensional (3D) culture conditions. The current consensus is that 3D culture is better because it precisely mimics the cellular microenvironment in vivo. The biomodulatory effect of low‐intensity laser radiation (LILR) on cells in 2D cultures is already well established. On the other hand, the effect of this radiation on cells in 3D cultures has not yet been widely investigated. The aim of this work was to analyze the biomodulatory effect of LILR, at the wavelength (ƛ) of 685 nm, on the viability of osteogenic precursor cells (OPC) cultured as multicellular spheroids (MS). Agarose molds containning wells (300 μm diameter) were used for MS formation using the non‐adherent surface culture concept. A cell count (2×105) of the OPC lineage (MC3T3) was seeded in each mold and maintained under ideal growth conditions (Dulbecco's Modified...
The FASEB Journal
The aim of this study was to analyze the influence of low intensity laser irradiation at waveleng... more The aim of this study was to analyze the influence of low intensity laser irradiation at wavelengths (λ) of 670, 685 and 830 nm on the mitochondrial activity and gene expression profile of myogenic precursor cells (MPCs) in vitro. Primary rat MPCs were sown in multiwell dishes of 96 and 6 wells. Six experimental groups were set up: a non‐irradiated control group and five groups ranged by differing doses of radiation (0.3; 0.6; 0.9; 1.2 and 1.5 J/cm2). After 24 hours of irradiation, the mitochondrial activity was quantified using the colorimetric technique MTT‐formazan and established in terms of percentage in relation to the control. The gene expression profile was established through the quantitative analysis of the related isoforms IIa and IIx of the myosin heavy chain (MHC) by RT‐PCR five days after irradiation. The results showed that irradiation at λ of 670, 685 and 830 nm promoted respective increase of 56.8, 70.6 and 84.8 % on mitochondrial activity. These values were achieve...
Lasers in Medical Science
Cureus, May 22, 2023
Background Primary dysmenorrhea (PD) refers to the occurrence of painful menstrual cramps without... more Background Primary dysmenorrhea (PD) refers to the occurrence of painful menstrual cramps without pathological involvement of the pelvic organs, with considerable morbidity and high prevalence among females of reproductive age. Objective The objective of this study is to present and test the efficacy of an innovative method of interactive transcutaneous electrical nerve stimulation (iTENS) for PD. Methods and materials This study is a single-blind controlled clinical trial. This was conducted at the outpatient clinic of the faculty of physical therapy. Females with PD (n=124) were divided into the treated group (transcutaneous electrical nerve stimulation {TENS} group {TG}, n=62) and the placebo group (PG, n=62). A single session of either iTENS or placebo intervention was used for 35 minutes. Pain, the duration of analgesia, and the use of pain medication were assessed before and after the intervention. Data from before and after the treatment were compared between groups (Student's t-test). The level of significance was set at 5%. Results A significant decrease in pain (p<0.001) was observed after the intervention for the TG, with a more longlasting analgesia (p<0.001) and decreased need for pain medication (p<0.001). Conclusions The proposed method of transcutaneous electrical nerve stimulation (TENS) application showed positive results for pain management on females with PD, with no reported adverse effects. The new proposed TENS application takes into account the preferences of the patient regarding positioning and the number of channels needed to cause analgesia. This application was able to promote almost complete analgesia in females with primary dysmenorrhea, and the analgesia persisted for more than one menstrual cycle.
Journal of Photochemistry and Photobiology B-biology, May 1, 2019
Follicular cystic ovary disease is a common reproductive disorder in women and females of domesti... more Follicular cystic ovary disease is a common reproductive disorder in women and females of domestic animals, characterized by anovulation and the persistence of follicle is a common cause of reproductive failure in mammalian. Polycystic ovary syndrome (PCOS) is characterized by hyperandrogenism (HA), chronic anovulation and polycystic ovaries, and it is a common reproductive endocrine disease with clinical manifestations including hirsutism, acne, infertility and obesity that can affect 5-20% of women in their reproductive age. Photobiomodulation (PBM) has been investigated and used in clinical practice, related to biomodulatory influences on cellular functions in animals and humans, both in vivo and in vitro. In this study, we include endocrine and reproductive features in a rat model for PCOS and the effects of PBM on ovarian activities. Forty-five adult female Wistar rats PCOS-induced by a single dose of the estradiol valerate (EV) were used in the study. After the EV injection for PCO induction, rats were divided into 9 groups (n = 5/group) named C30, C45 and C60 (Control group), S30, S45 and S60 (PCO group) and L30, L45 and L60 (PCO/Laser group). The rats were irradiated with laser 3 times/week. The results shown that EV PCO-induced rats had increased body mass, reduced ovary mass, and reduced GSI. The plasma levels of P4 and T were increased, and the LH plasma level was decreased by PBM stimulation. The number of ovarian follicles and corpus luteum were increased, and the number of ovarian cysts was decreased by PBM stimulation. Thus, reproductive and endocrine characteristics were modulated by PBM.
Hansenologia Internationalis, Nov 29, 2000
A hanseníase apresenta, como incapacidade decorrente muito comum, a ulceração plantar. Tendo em v... more A hanseníase apresenta, como incapacidade decorrente muito comum, a ulceração plantar. Tendo em vista a necessidade de implantação de programas de ação de controle da doença e melhora da qualidade de vida dos portadores desta nos serviços básicos de saúde, este projeto viabilizou, comparativamente a afetados pela mesma lesão, porém de outras etiologias, a utilização da laserterapia de baixa intensidade para cicatrização das úlceras hansênicas de membros inferiores. Na sua adoção, uma vez claramente definidos população de referência, bem como respectivos critérios de inclusão e exclusão, a normalização ética vigente no país prevista foi rigorosamente cumprida. Promoveram-se procedimentos adequados para padronização de condutas de mensuração, avaliação, aplicação e acompanhamento da 1 Projeto parcialmente financiado pela Fundação Paulista Contra Hanseníase (FPCH) e apoiado por bolsa da Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES);
The FASEB Journal
Recent research evidences substantial morphological and physiological differences between cells m... more Recent research evidences substantial morphological and physiological differences between cells maintained in vitro in two‐dimensional (2D) and three‐dimensional (3D) culture conditions. The current consensus is that 3D culture is better because it precisely mimics the cellular microenvironment in vivo. The biomodulatory effect of low‐intensity laser radiation (LILR) on cells in 2D cultures is already well established. On the other hand, the effect of this radiation on cells in 3D cultures has not yet been widely investigated. The aim of this work was to analyze the biomodulatory effect of LILR, at the wavelength (ƛ) of 685 nm, on the viability of osteogenic precursor cells (OPC) cultured as multicellular spheroids (MS). Agarose molds containning wells (300 μm diameter) were used for MS formation using the non‐adherent surface culture concept. A cell count (2×105) of the OPC lineage (MC3T3) was seeded in each mold and maintained under ideal growth conditions (Dulbecco's Modified...
The FASEB Journal
The aim of this study was to analyze the influence of low intensity laser irradiation at waveleng... more The aim of this study was to analyze the influence of low intensity laser irradiation at wavelengths (λ) of 670, 685 and 830 nm on the mitochondrial activity and gene expression profile of myogenic precursor cells (MPCs) in vitro. Primary rat MPCs were sown in multiwell dishes of 96 and 6 wells. Six experimental groups were set up: a non‐irradiated control group and five groups ranged by differing doses of radiation (0.3; 0.6; 0.9; 1.2 and 1.5 J/cm2). After 24 hours of irradiation, the mitochondrial activity was quantified using the colorimetric technique MTT‐formazan and established in terms of percentage in relation to the control. The gene expression profile was established through the quantitative analysis of the related isoforms IIa and IIx of the myosin heavy chain (MHC) by RT‐PCR five days after irradiation. The results showed that irradiation at λ of 670, 685 and 830 nm promoted respective increase of 56.8, 70.6 and 84.8 % on mitochondrial activity. These values were achieve...
Este trabalho apresenta uma serie de resultados referentes a utilizacao da Microscopia de Forca A... more Este trabalho apresenta uma serie de resultados referentes a utilizacao da Microscopia de Forca Atomica (MFA) em aplicacoes na observacao de materiais biologicos, comprovando sua eficacia como importante metodo para investigacao de variados fenomenos, A estrutura molecular do colageno do tendao de rato foi utilizado como modelo de reparacao teciduaL Utilizou-se laser de ReNe (6 mW) como recurso no intuito de promover ou facilitar a cicatrizacao e se analisou por meio da MFA, Microscopia Eletronica de Varredura (MEV) e Espectroscopia de Infravermelho por Transformada de Fourier (FT-IR), estes ultimos com objetivos de dar um panorama de menor ampliacao do tecido em reparacao e avaliar os aspectos das possiveis modificacoes bioquimicas na estrutura das moleculas de colageno, respectivamente. A MEV mostrou evidencias de melhor organizacao na reparacao do tendao nos animais tratados com o laser de ReNe, acelerando-a em relacao aos animais controle, nao tratados. Os animais tratados pelo laser com diferentes doses, mostrou sinais de aceleracao do processo na dependencia da dose. As doses que foram mais eficientes foram 5 J/cm2, 50 J/cm2e 0,5 J/cm2 considerados em ordem decrescente. Tais resultados foram corroborados com a MFA, repetindo as caracteristicas de auto enrolamento, mesmo quando observado em resolucoes maiores. Nao houve modificacoes dignas de nota quando comparados os resultados obtidos com FT-IR. Provavelmente houve alguma falha na preparacao das amostra para este objetivo, uma vez que as amostras eram as mesmas preparadas para analises no MFA... Observacao: O resumo, na integra, podera ser visualizado no texto completo da tese digital Abstract
Lasers in Medical Science
Journal of Photochemistry and Photobiology B-biology, Mar 1, 2020
Cureus, May 22, 2023
Background Primary dysmenorrhea (PD) refers to the occurrence of painful menstrual cramps without... more Background Primary dysmenorrhea (PD) refers to the occurrence of painful menstrual cramps without pathological involvement of the pelvic organs, with considerable morbidity and high prevalence among females of reproductive age. Objective The objective of this study is to present and test the efficacy of an innovative method of interactive transcutaneous electrical nerve stimulation (iTENS) for PD. Methods and materials This study is a single-blind controlled clinical trial. This was conducted at the outpatient clinic of the faculty of physical therapy. Females with PD (n=124) were divided into the treated group (transcutaneous electrical nerve stimulation {TENS} group {TG}, n=62) and the placebo group (PG, n=62). A single session of either iTENS or placebo intervention was used for 35 minutes. Pain, the duration of analgesia, and the use of pain medication were assessed before and after the intervention. Data from before and after the treatment were compared between groups (Student's t-test). The level of significance was set at 5%. Results A significant decrease in pain (p<0.001) was observed after the intervention for the TG, with a more longlasting analgesia (p<0.001) and decreased need for pain medication (p<0.001). Conclusions The proposed method of transcutaneous electrical nerve stimulation (TENS) application showed positive results for pain management on females with PD, with no reported adverse effects. The new proposed TENS application takes into account the preferences of the patient regarding positioning and the number of channels needed to cause analgesia. This application was able to promote almost complete analgesia in females with primary dysmenorrhea, and the analgesia persisted for more than one menstrual cycle.
Fisioterapia Brasil, Dec 8, 2016
Resumo O objetivo desta revisão é complementar os dados revisados na parte 1 onde foram discutido... more Resumo O objetivo desta revisão é complementar os dados revisados na parte 1 onde foram discutidos princípios e generalidades, disponibilizar ao leitor referencial teórico para conhecer a interação do laser de baixa intensidade com os diferentes tipos de tecidos e analisar as distintas respostas. Este material constitui um acervo atualizado onde se especificam os diversos parâmetros aplicados e a técnica utilizados em cada trabalho, o qual vai servir para que os profissionais interessadas nesta área direcionem melhor suas pesquisas e determinem com maior precisão as doses para o tratamento de seus pacientes ao contar com diferentes alternativas.
Fisioterapia Brasil, Dec 8, 2016
O estresse faz-se presente em todas as reações do organismo desencadeadas por agressões de ordem ... more O estresse faz-se presente em todas as reações do organismo desencadeadas por agressões de ordem física, psíquica ou infecciosa. Situações de grande tensão pode provocar o estresse patológico, facilitando ou agravando distúrbios transitórios ou doenças graves. Verificaram-se os efeitos do Watsu em dezoito pacientes sob experimentação com características de estresse, submetendo-os aos procedimentos descritos pelos autores para a referida técnica hidroterápica. As diversas posições recomendadas pela técnica associadas aos benefícios da água aquecida mostrou promover momentos de profundo relaxamento. Mais de 50% dos participantes já havia realizado tratamento fisioterápico clássico com resultados insatisfatórios. Com o Watsu®, além dos pacientes relatarem melhoras das algias musculares, acrescentaram que houve um aumento no rendimento do trabalho, maior disposição para atividades da vida diária, mais flexibilidade e ponderação para resolver situações conflitantes, enfim uma melhora na homeostase. Pode-se concluir que é vantajosa a associação da hidroterapia com água hipertérmica e o uso de exercícios e alongamentos pela técnica de Watsu para controle do estresse.
Journal of Photochemistry and Photobiology B-biology, Dec 1, 2019
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
PubMed, May 14, 2010
Background: Ultrasound (US) is a widely used and studied resource for physical therapy treatments... more Background: Ultrasound (US) is a widely used and studied resource for physical therapy treatments. Given the scarcity of studies on the effects of US on healthy tissue, many physical therapy professionals make unfounded decisions regarding its methods and parameters of application. Objectives: The possible histological and morphometric changes in the healthy tissue of Wistar rats in vivo irradiated with different intensities of US were evaluated. Methods: Thirty Wistar rats, randomly distributed among five groups of six animals each, were treated on the right side of the dorsal region, over an area of 4 cm(2). The left side served as a control. The treatment was applied over a four-day period, with two mins. of daily irradiation. The output intensity was checked using a precision dosimeter before the applications. Histological and morphometric analyses were performed using the Image Tool software. Results: There were slight inflammatory infiltration and thinning of the dermis fibers, particularly in the groups irradiated with 1.5 and 2 W/cm(2). There was also thickening of the epidermis in the samples from the irradiated animals. To evaluate the quantitative results, the statistical analyses consisted of one-way ANOVAs with the post-hoc Tukey tests. There were significant differences in epidermis thicknesses between the control group and the groups irradiated with 1.0, 1.5 and 2.0 W/cm(2). Conclusions: Higher doses of US produced changes in the epidermis and dermis, i.e. increased thickness and collagen fiber thinning and proliferation, respectively. These results serve as a warning of the possible implications of therapeutic ultrasound use in esthetics.
Pain Practice, Apr 1, 2020
There is a wide range of animal models available today for studying chronic pain associated with ... more There is a wide range of animal models available today for studying chronic pain associated with a variety of etiologies and an extensive list of clinical manifestations of peripheral neuropathies. Photobiomodulation is a new tool for the treatment of pain in a convenient, non-invasive way. The aim of this work is to elucidate the effects of infrared light emitting diodes (LEDs) on behavioral responses to nociceptive stimuli in chronic pain models. Forty-eight Swiss male mice weighing 25-35 g were used. Two chronic pain models, ischemia-reperfusion (IR) and spared spinal nerve injury (SNI), were performed and then treated with infrared LED irradiation (390 mW, 890 nm, 17.3 mW/cm², 20.8 J/cm², for 20 min). The behavioral tests used were a mechanical hypersensitivity test (von Frey test) and a cold allodynia test (acetone test). The results showed that, in the ischemia-reperfusion model, the infrared LED had a significant effect on mechanical stimulation and cold allodynia on every day of treatment. In the spared nerve injury model, an analgesic effect was observed on every treatment day (when started on the 3 rd and 7 th days after the surgery). In both models, the effect was abolished when the treatment was interrupted. These findings suggest that photobiomodulation therapy may be a useful adjunct treatment for chronic pain.