Noel Roble - Academia.edu (original) (raw)
Papers by Noel Roble
Ganoderma lucidum (W. Curt: Fr.) P. Karst is a popular mushroom in oriental medicine that has lon... more Ganoderma lucidum (W. Curt: Fr.) P. Karst is a popular mushroom in oriental medicine that has long been used as ingredient in health foods and drugs as it contains various bioactive metabolites. Several cultivation methods are established to obtain high yields, high quality of G. lucidum . This study determines the possibility of using mango peel powder in the production of mycelial biomass of G. lucidum in a modified airlift bioreactor with varying aeration rate. The final 5 concentrations (5g/L, 10g/L, 15g/L, 20g/L, and 25g/L) of the mango peel powder with starch were used in the final investigation. Mycelial biomass yield was higher in 10g/L without starch addition at 3.88, 4.98 and 5.01 mg/mL, at 0.5 vvm, 1.0 vvm 1.5vvm aeration rate respectively. Similarly 10g/L mango peel powder with 90g starch gave the highest biomass of 10.98, 19.32 and 16.01 mg/mL, at 0.5 vvm, 1.0 vvm 1.5vvm aeration rate, respectively. The final 5 concentrations of the mango peel powder were 5g/L, 10g/L, 1...
We conducted this study to assess how well a pupal survey of dengue mosquito vectors, Aedes aegyp... more We conducted this study to assess how well a pupal survey of dengue mosquito vectors, Aedes aegypti and Aedes albopictus, is able to target the most productive breeding sites. The study was carried out monthly during the rainy season (8 months) in 2008 in Cuba, Cebu City, Philippines. The hypotheses tested were: 1) most pupae of Ae. aegypti or Ae. albopictus were produced in a few types of breeding sites and 2) the most productive types of breeding sites for each species were the most abundant. Approximately 2,500 pupae were collected from 554 breeding sites in 279 houses. Thirty-eight point four percent of ten types of breeding sites were positive for Ae. aegypti, and 11.9% of nine types of sites were positive for Ae. albopictus. Plastic drums (40.2%), metal drums (29.6%), and plastic containers (10.5%) were the key sites for Ae. aegypti pupae, whereas bamboo stumps (28.5%), plastic drums (21.1%), and rubber tires (19.1%) were the key sites for Ae. albopictus. The most productive b...
Process Biochemistry
Abstract As a solution to the problems of mass transfer limitation in submerged cultures and scal... more Abstract As a solution to the problems of mass transfer limitation in submerged cultures and scale up of solid-state/liquid-surface cultures, an alternating liquid phase–air phase bioreactor was developed. It consisted of a bioreactor equipped with a siphon system and a reservoir. Aspergillus awamori was immobilized in loofa sponge inside the bioreactor and culture broth was pumped from the reservoir into the bioreactor. Each time the culture broth level reached a critical level, the broth automatically siphoned back into the reservoir. Thus the immobilized cells were alternatingly submerged and exposed to air. The duration of each phase was controlled by the pumping rate and with an on-off timer. During amylase production from soluble starch and raw cassava starch, the optima ratios of the liquid to air phases were 12 h : 12 h and 3 h : 6 h respectively. Saccharomyces cerevisiae IR2 was immobilized in the reservoir and the system was used for simultaneous amylase production, hydrolysis and ethanol production from raw cassava starch. The process was very stable for more than 7 batches with high ethanol yield of 0.46 g-ethanol/g-starch and productivity of 1.73 g-ethanol/L/h. These values are high, the system can be scaled up, and thus it has many potential applications.
Journal of Aquatic Science, 2016
The presence of seawater bugs in the coastal area is an indicator of good water quality and stabl... more The presence of seawater bugs in the coastal area is an indicator of good water quality and stable mangrove habitat. As energy source of other organisms, their role in the food chain (Chen et al., 2005) makes them important in the marine ecosystem. This study was conducted in order to know the species occurrence, abundance and distribution and their relationships with the physicochemical parameters such as light, humidity, temperature, pH, salinity, dissolved oxygen, nitrite, nitrate and phosphate that would affect their population to fluctuate or change. In the sampling sites, a quadrat sampling method was used for collections and gathering of data. Results indicated that 824 adult bugs composed of 4 Families, 8 Genera and 13 Species were found. The species were Halobates calyptus, Halobates hayanus, Halobates proavus, Pseudohalobates inobonto and Stenobates sangihi of the Family Gerridae; Haloveloides femoralis, Halovelia esakii, Halovelia malaya, Halovelia bergrothii, Xenobates s...
日本生物工学会大会講演要旨集, Sep 25, 2002
Applied Microbiology and Biotechnology, 2003
Biotechnology Letters - BIOTECHNOL LETT, 2003
l-Lactic acid was produced from raw cassava starch, by simultaneous enzyme production, starch sac... more l-Lactic acid was produced from raw cassava starch, by simultaneous enzyme production, starch saccharification and fermentation in a circulating loop bioreactor with Aspergillus awamori and Lactococcus lactis spp. lactis immobilized in loofa sponge. A. awamori was immobilized directly in cylindrical loofa sponge while the L. lactis was immobilized in a loofa sponge alginate gel cube. In the loofa sponge alginate gel cube, the sponge serves as skeletal support for the gel with the cells. The alginate gel formed a hard outer layer covering the soft porous gel inside. By controlling the rate and frequency of broth circulation between the riser and downcomer columns, the riser could be maintained under aerobic condition while the downcomer was under anaerobic condition. Repeated fed-batch l-lactic acid production was performed for more than 400 h and the average lactic acid yield and productivity from raw cassava starch were 0.76 g lactic acid g-1 starch and 1.6 g lactic acid l-1 h-1, r...
Southeast Asian Journal of Tropical Medicine and Public Health, 2012
We conducted this study to assess how well a pupal survey of dengue mosquito vectors, Aedes aegyp... more We conducted this study to assess how well a pupal survey of dengue mosquito vectors, Aedes aegypti and Aedes albopictus, is able to target the most productive breeding sites. The study was carried out monthly during the rainy season (8 months) in 2008 in Cuba, Cebu City, Philippines. The hypotheses tested were: 1) most pupae of Ae. aegypti or Ae. albopictus were produced in a few types of breeding sites and 2) the most productive types of breeding sites for each species were the most abundant. Approximately 2,500 pupae were collected from 554 breeding sites in 279 houses. Thirty-eight point four percent of ten types of breeding sites were positive for Ae. aegypti, and 11.9% of nine types of sites were positive for Ae. albopictus. Plastic drums (40.2%), metal drums (29.6%), and plastic containers (10.5%) were the key sites for Ae. aegypti pupae, whereas bamboo stumps (28.5%), plastic drums (21.1%), and rubber tires (19.1%) were the key sites for Ae. albopictus. The most productive b...
Ganoderma lucidum (W. Curt: Fr.) P. Karst is a popular mushroom in oriental medicine that has lon... more Ganoderma lucidum (W. Curt: Fr.) P. Karst is a popular mushroom in oriental medicine that has long been used as ingredient in health foods and drugs as it contains various bioactive metabolites. Several cultivation methods are established to obtain high yields, high quality of G. lucidum . This study determines the possibility of using mango peel powder in the production of mycelial biomass of G. lucidum in a modified airlift bioreactor with varying aeration rate. The final 5 concentrations (5g/L, 10g/L, 15g/L, 20g/L, and 25g/L) of the mango peel powder with starch were used in the final investigation. Mycelial biomass yield was higher in 10g/L without starch addition at 3.88, 4.98 and 5.01 mg/mL, at 0.5 vvm, 1.0 vvm 1.5vvm aeration rate respectively. Similarly 10g/L mango peel powder with 90g starch gave the highest biomass of 10.98, 19.32 and 16.01 mg/mL, at 0.5 vvm, 1.0 vvm 1.5vvm aeration rate, respectively. The final 5 concentrations of the mango peel powder were 5g/L, 10g/L, 1...
We conducted this study to assess how well a pupal survey of dengue mosquito vectors, Aedes aegyp... more We conducted this study to assess how well a pupal survey of dengue mosquito vectors, Aedes aegypti and Aedes albopictus, is able to target the most productive breeding sites. The study was carried out monthly during the rainy season (8 months) in 2008 in Cuba, Cebu City, Philippines. The hypotheses tested were: 1) most pupae of Ae. aegypti or Ae. albopictus were produced in a few types of breeding sites and 2) the most productive types of breeding sites for each species were the most abundant. Approximately 2,500 pupae were collected from 554 breeding sites in 279 houses. Thirty-eight point four percent of ten types of breeding sites were positive for Ae. aegypti, and 11.9% of nine types of sites were positive for Ae. albopictus. Plastic drums (40.2%), metal drums (29.6%), and plastic containers (10.5%) were the key sites for Ae. aegypti pupae, whereas bamboo stumps (28.5%), plastic drums (21.1%), and rubber tires (19.1%) were the key sites for Ae. albopictus. The most productive b...
Process Biochemistry
Abstract As a solution to the problems of mass transfer limitation in submerged cultures and scal... more Abstract As a solution to the problems of mass transfer limitation in submerged cultures and scale up of solid-state/liquid-surface cultures, an alternating liquid phase–air phase bioreactor was developed. It consisted of a bioreactor equipped with a siphon system and a reservoir. Aspergillus awamori was immobilized in loofa sponge inside the bioreactor and culture broth was pumped from the reservoir into the bioreactor. Each time the culture broth level reached a critical level, the broth automatically siphoned back into the reservoir. Thus the immobilized cells were alternatingly submerged and exposed to air. The duration of each phase was controlled by the pumping rate and with an on-off timer. During amylase production from soluble starch and raw cassava starch, the optima ratios of the liquid to air phases were 12 h : 12 h and 3 h : 6 h respectively. Saccharomyces cerevisiae IR2 was immobilized in the reservoir and the system was used for simultaneous amylase production, hydrolysis and ethanol production from raw cassava starch. The process was very stable for more than 7 batches with high ethanol yield of 0.46 g-ethanol/g-starch and productivity of 1.73 g-ethanol/L/h. These values are high, the system can be scaled up, and thus it has many potential applications.
Journal of Aquatic Science, 2016
The presence of seawater bugs in the coastal area is an indicator of good water quality and stabl... more The presence of seawater bugs in the coastal area is an indicator of good water quality and stable mangrove habitat. As energy source of other organisms, their role in the food chain (Chen et al., 2005) makes them important in the marine ecosystem. This study was conducted in order to know the species occurrence, abundance and distribution and their relationships with the physicochemical parameters such as light, humidity, temperature, pH, salinity, dissolved oxygen, nitrite, nitrate and phosphate that would affect their population to fluctuate or change. In the sampling sites, a quadrat sampling method was used for collections and gathering of data. Results indicated that 824 adult bugs composed of 4 Families, 8 Genera and 13 Species were found. The species were Halobates calyptus, Halobates hayanus, Halobates proavus, Pseudohalobates inobonto and Stenobates sangihi of the Family Gerridae; Haloveloides femoralis, Halovelia esakii, Halovelia malaya, Halovelia bergrothii, Xenobates s...
日本生物工学会大会講演要旨集, Sep 25, 2002
Applied Microbiology and Biotechnology, 2003
Biotechnology Letters - BIOTECHNOL LETT, 2003
l-Lactic acid was produced from raw cassava starch, by simultaneous enzyme production, starch sac... more l-Lactic acid was produced from raw cassava starch, by simultaneous enzyme production, starch saccharification and fermentation in a circulating loop bioreactor with Aspergillus awamori and Lactococcus lactis spp. lactis immobilized in loofa sponge. A. awamori was immobilized directly in cylindrical loofa sponge while the L. lactis was immobilized in a loofa sponge alginate gel cube. In the loofa sponge alginate gel cube, the sponge serves as skeletal support for the gel with the cells. The alginate gel formed a hard outer layer covering the soft porous gel inside. By controlling the rate and frequency of broth circulation between the riser and downcomer columns, the riser could be maintained under aerobic condition while the downcomer was under anaerobic condition. Repeated fed-batch l-lactic acid production was performed for more than 400 h and the average lactic acid yield and productivity from raw cassava starch were 0.76 g lactic acid g-1 starch and 1.6 g lactic acid l-1 h-1, r...
Southeast Asian Journal of Tropical Medicine and Public Health, 2012
We conducted this study to assess how well a pupal survey of dengue mosquito vectors, Aedes aegyp... more We conducted this study to assess how well a pupal survey of dengue mosquito vectors, Aedes aegypti and Aedes albopictus, is able to target the most productive breeding sites. The study was carried out monthly during the rainy season (8 months) in 2008 in Cuba, Cebu City, Philippines. The hypotheses tested were: 1) most pupae of Ae. aegypti or Ae. albopictus were produced in a few types of breeding sites and 2) the most productive types of breeding sites for each species were the most abundant. Approximately 2,500 pupae were collected from 554 breeding sites in 279 houses. Thirty-eight point four percent of ten types of breeding sites were positive for Ae. aegypti, and 11.9% of nine types of sites were positive for Ae. albopictus. Plastic drums (40.2%), metal drums (29.6%), and plastic containers (10.5%) were the key sites for Ae. aegypti pupae, whereas bamboo stumps (28.5%), plastic drums (21.1%), and rubber tires (19.1%) were the key sites for Ae. albopictus. The most productive b...