Noemi Torres - Academia.edu (original) (raw)
Papers by Noemi Torres
Current Medicinal Chemistry, 2018
Chronic kidney disease (CKD) is a progressive condition characterized by a permanent and irrevers... more Chronic kidney disease (CKD) is a progressive condition characterized by a permanent and irreversible loss of renal function. In accordance to international guidelines, CKD clinical diagnosis methods are based on creatinine and albumin levels and glomerular filtration rate. Unfortunately, these parameters are scarcely affected in early stages, and its inherent intrinsic variability only allows for the identification of intermediate and advanced stages, when life expectancy has become shorter and treatment poses a significant financial investment. In this context, several targeted strategies have been designed for searching novel markers. Among them, "omics" techniques have emerged, mainly based on proteomics and metabolomics research. Urine and serum samples have been selected as starting material to conduct the identification of new CKD biomarkers, capable of differentiating between stages and predicting progression outcomes. In many cases, the principal objective is to d...
Educación Química, 2019
Nuclear Magnetic Resonance spectroscopy is a high versatile analysis method which has contributed... more Nuclear Magnetic Resonance spectroscopy is a high versatile analysis method which has contributed in different fields of scientific knowledge. Initially, its discovery as physical phenomenon caught notable interest. However, applications in chemistry and recently in medicine have had a deeper impact. In a historic context is important to recognize personal contributions of developers of this area of spectroscopy, which is still now generating theoretical and experimental knowledge.
BMC complementary and alternative medicine, Jan 13, 2017
In vitro bioassays are important in the evaluation of plants with possible hepatoprotective effec... more In vitro bioassays are important in the evaluation of plants with possible hepatoprotective effects. The aims of this study were to evaluate the pretreatment of HepG2 cells with hepatoprotective agents against the damage induced by carbon tetrachloride (CCl4) and paracetamol (APAP). Antioxidative activity was measured using an assay to measure 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. The in vitro hepatotoxicity of CCl4 and APAP, and the cytotoxic and hepatoprotective properties of silymarin (SLM), silybinin (SLB), and silyphos (SLP) were evaluated by measuring cell viability; activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH); total antioxidant capacity (TAOxC); and reduced glutathione (GSH), superoxide dismutase (SOD), and lipid peroxidation (malondialdehyde (MDA) levels). Only SLB and SLM showed strong antioxidative activity in the DPPH assay (39.71 ± 0.85 μg/mL and 14.14 ± 0.65 μg/mL, respectively)....
Medicina Universitaria, 2015
Liver diseases are a major health problem worldwide, making it necessary to develop new molecules... more Liver diseases are a major health problem worldwide, making it necessary to develop new molecules that help counteract or prevent such diseases. On account of this fact, investigations aiming to obtain natural and/or synthetic compounds possessing hepatoprotective activity have been undertaken. The development of new drugs consists of a variety of steps, ranging from the discovery of the pharmacological effects in cellular and animal models, to finally demonstrate their efficacy and safety in humans. Different models for assessment of the hepatoprotective activity in vitro, ex vivo and in vivo can be found in medical literature. The purpose of this review is to show the features, main advantages and disadvantages of each of the models, the hepatotoxic agents most commonly used (CCl 4 , acetaminophen, ethanol, dgalactosamine, t-BuOOH, thioacetamide) as well as the biochemical parameters useful to assess liver damage in the different models.
Gac Med Mex, Feb 1, 1995
A 48-year-old woman was diagnosed with left-sided breast cancer. The initial operation entailed l... more A 48-year-old woman was diagnosed with left-sided breast cancer. The initial operation entailed left mastectomy, sentinel lymph node biopsy, and tissue expander (TE) insertion. On postoperative day 1 (POD1), bloody discharge from the chest drain was observed and it continuously reduced under compression. However, between POD13 and POD14, the amount of discharge increased (Fig. 1). A computed tomography scan of the chest revealed a hematoma underneath the left pectoralis major muscle (Fig. 2). The next day, POD14, 250 mL of hematoma was removed. No further bleeding occurred, and the patient was discharged on POD20 (Fig. 3). Ten months later, breast reconstruction was performed using a deep inferior epigastric perforator (DIEP) flap.
Molecules (Basel, Switzerland), Jan 21, 2015
Seventeen new derivatives of the natural diterpene leubethanol, including some potential pro-drug... more Seventeen new derivatives of the natural diterpene leubethanol, including some potential pro-drugs, with changes in the functionality of the aliphatic chain or modifications of aromatic ring and the phenolic group, were synthesized and tested in vitro by the MABA technique for their activity against the H37Rv strain of Mycobacterium tuberculosis. Some compounds showed antimycobacterial selectivity indices higher than leubethanol.
PLoS Neglected Tropical Diseases, 2008
Background: Mycetoma is a chronic infectious disease of tropical and subtropical countries. It is... more Background: Mycetoma is a chronic infectious disease of tropical and subtropical countries. It is produced by true fungi and actinobacteria. In México, Nocardia brasiliensis is the main causative agent of mycetoma, producing about 86% of the cases; the gold standard for the therapy of mycetoma by N. brasiliensis is the use of sulfonamides which give a 70% cure rate. The addition of amikacin to this regime increases to 95% the cure rate; however, the patients have to be monitored for creatinine clearance and audiometry studies because of the potential development of side effects. Because of that it is important to search for new active compounds. In the present work, we evaluated the in vivo effect of DA-7867, an experimental oxazolidinone, on the development of experimental mycetomas by N. brasiliensis in BALB/c mice. Methodology/Principal Findings: In order to determine the optimal dose utilized to apply to the animals, we first determined by HPLC the plasma levels using several concentrations of the compounds. Based on these results, we used 10 and 25 mg/kg subcutaneously every 24 hr; DA-7867 was also supplied in the drinking water at a calculated dose of 25 mg/ kg. As a control we utilized linezolid at 25 mg/kg, a compound active in murine and human infections, three times a day. The mice were infected in the right footpad with a young culture of N. brasiliensis HUJEG-1, and one week later we started the application of the antimicrobials for six more weeks. After that we compared the development of lesions in the groups injected with saline solution or with the antimicrobials; the results were analyzed by the variance ANOVA test. DA-7867 was able to reduce the production of lesions at 25 mg/kg, when given either subcutaneously or in the drinking water. Conclusions/Significance: The experimental oxazolidinone DA-7867 is active in vivo against N. brasiliensis, which opens the possibility of using this drug once it is accepted for human application. Since oxazolidinones seem to be active against a wide spectrum of actinobacteria, it is possible they could be used in human cases of mycetoma by other actinomycetales, such as Streptomyces somaliensis, highly prevalent in Sudan, or Actinomadura madurae and A. pelletieri, which are commonly observed in Africa and India.
Food Additives and Contaminants: Part B, 2010
Dioxins in beef were quantified by high resolution gas chromatography coupled to low-resolution m... more Dioxins in beef were quantified by high resolution gas chromatography coupled to low-resolution mass spectrometry (GC/LRMS). The analyses were performed according to the minimum requirements described in the USEPA 1613 method with some minor modifications. Levels found in the samples were in the range 1.02-8.04 pg WHO-TEQ PCDDs/PCDFs g(-1) fat. For comparison purposes, the maximum level allowed by the European Union is 3 pg WHO-TEQ PCDDs/PCDFs g(-1) fat, and some of these samples surpassed the above-mentioned limit and can be considered as contaminated food. The results confirm that a preliminary screening of dioxins in beef can be performed by GC/LRMS. As far as we know, this is the first report of dioxins in beef in Mexico. After the appropriated tests, the applied methodology could be considered as an alternative screening method for the analysis of PCDD/Fs in other food products.
Ciencia …, 2002
Resumen La T-514 es una antracenona dimérica obtenida de plantas del género Karwinskia. Este comp... more Resumen La T-514 es una antracenona dimérica obtenida de plantas del género Karwinskia. Este compuesto posee toxicidad selectiva in vitro hacia células de origen tumoral y es considerado como un potencial agente antineoplásico. En este estudio se ...
Antimicrobial Agents and Chemotherapy, 2008
Analytical Letters, 2008
... la Luz Salazar‐Cavazos a , Noemí H. Waksman de Torres a & Rocío Castro‐Ríos a * p... more ... la Luz Salazar‐Cavazos a , Noemí H. Waksman de Torres a & Rocío Castro‐Ríos a * pages 119-136. ... J. Envir. Anal. Chemi. , 87: 211225. [Taylor & Francis Online], [Web of Science ®] View all references; Reza et al. 200626. Reza, A., Hosseinzadeh, S. and Pawliszyn, J. 2006. ...
BMC Complementary and Alternative Medicine
Current Analytical Chemistry
Resumen Las técnicas empleadas para el control de calidad de la Peroxisomicina A1, fueron mejorad... more Resumen Las técnicas empleadas para el control de calidad de la Peroxisomicina A1, fueron mejoradas aplicando técnicas quimiométricas tanto en CLAR como en espectrofotometría UV-visible. El uso de cromatogramas derivados en CLAR, permitió ...
Planta Medica, Jun 1, 2011
Current Medicinal Chemistry, 2018
Chronic kidney disease (CKD) is a progressive condition characterized by a permanent and irrevers... more Chronic kidney disease (CKD) is a progressive condition characterized by a permanent and irreversible loss of renal function. In accordance to international guidelines, CKD clinical diagnosis methods are based on creatinine and albumin levels and glomerular filtration rate. Unfortunately, these parameters are scarcely affected in early stages, and its inherent intrinsic variability only allows for the identification of intermediate and advanced stages, when life expectancy has become shorter and treatment poses a significant financial investment. In this context, several targeted strategies have been designed for searching novel markers. Among them, "omics" techniques have emerged, mainly based on proteomics and metabolomics research. Urine and serum samples have been selected as starting material to conduct the identification of new CKD biomarkers, capable of differentiating between stages and predicting progression outcomes. In many cases, the principal objective is to d...
Educación Química, 2019
Nuclear Magnetic Resonance spectroscopy is a high versatile analysis method which has contributed... more Nuclear Magnetic Resonance spectroscopy is a high versatile analysis method which has contributed in different fields of scientific knowledge. Initially, its discovery as physical phenomenon caught notable interest. However, applications in chemistry and recently in medicine have had a deeper impact. In a historic context is important to recognize personal contributions of developers of this area of spectroscopy, which is still now generating theoretical and experimental knowledge.
BMC complementary and alternative medicine, Jan 13, 2017
In vitro bioassays are important in the evaluation of plants with possible hepatoprotective effec... more In vitro bioassays are important in the evaluation of plants with possible hepatoprotective effects. The aims of this study were to evaluate the pretreatment of HepG2 cells with hepatoprotective agents against the damage induced by carbon tetrachloride (CCl4) and paracetamol (APAP). Antioxidative activity was measured using an assay to measure 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. The in vitro hepatotoxicity of CCl4 and APAP, and the cytotoxic and hepatoprotective properties of silymarin (SLM), silybinin (SLB), and silyphos (SLP) were evaluated by measuring cell viability; activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH); total antioxidant capacity (TAOxC); and reduced glutathione (GSH), superoxide dismutase (SOD), and lipid peroxidation (malondialdehyde (MDA) levels). Only SLB and SLM showed strong antioxidative activity in the DPPH assay (39.71 ± 0.85 μg/mL and 14.14 ± 0.65 μg/mL, respectively)....
Medicina Universitaria, 2015
Liver diseases are a major health problem worldwide, making it necessary to develop new molecules... more Liver diseases are a major health problem worldwide, making it necessary to develop new molecules that help counteract or prevent such diseases. On account of this fact, investigations aiming to obtain natural and/or synthetic compounds possessing hepatoprotective activity have been undertaken. The development of new drugs consists of a variety of steps, ranging from the discovery of the pharmacological effects in cellular and animal models, to finally demonstrate their efficacy and safety in humans. Different models for assessment of the hepatoprotective activity in vitro, ex vivo and in vivo can be found in medical literature. The purpose of this review is to show the features, main advantages and disadvantages of each of the models, the hepatotoxic agents most commonly used (CCl 4 , acetaminophen, ethanol, dgalactosamine, t-BuOOH, thioacetamide) as well as the biochemical parameters useful to assess liver damage in the different models.
Gac Med Mex, Feb 1, 1995
A 48-year-old woman was diagnosed with left-sided breast cancer. The initial operation entailed l... more A 48-year-old woman was diagnosed with left-sided breast cancer. The initial operation entailed left mastectomy, sentinel lymph node biopsy, and tissue expander (TE) insertion. On postoperative day 1 (POD1), bloody discharge from the chest drain was observed and it continuously reduced under compression. However, between POD13 and POD14, the amount of discharge increased (Fig. 1). A computed tomography scan of the chest revealed a hematoma underneath the left pectoralis major muscle (Fig. 2). The next day, POD14, 250 mL of hematoma was removed. No further bleeding occurred, and the patient was discharged on POD20 (Fig. 3). Ten months later, breast reconstruction was performed using a deep inferior epigastric perforator (DIEP) flap.
Molecules (Basel, Switzerland), Jan 21, 2015
Seventeen new derivatives of the natural diterpene leubethanol, including some potential pro-drug... more Seventeen new derivatives of the natural diterpene leubethanol, including some potential pro-drugs, with changes in the functionality of the aliphatic chain or modifications of aromatic ring and the phenolic group, were synthesized and tested in vitro by the MABA technique for their activity against the H37Rv strain of Mycobacterium tuberculosis. Some compounds showed antimycobacterial selectivity indices higher than leubethanol.
PLoS Neglected Tropical Diseases, 2008
Background: Mycetoma is a chronic infectious disease of tropical and subtropical countries. It is... more Background: Mycetoma is a chronic infectious disease of tropical and subtropical countries. It is produced by true fungi and actinobacteria. In México, Nocardia brasiliensis is the main causative agent of mycetoma, producing about 86% of the cases; the gold standard for the therapy of mycetoma by N. brasiliensis is the use of sulfonamides which give a 70% cure rate. The addition of amikacin to this regime increases to 95% the cure rate; however, the patients have to be monitored for creatinine clearance and audiometry studies because of the potential development of side effects. Because of that it is important to search for new active compounds. In the present work, we evaluated the in vivo effect of DA-7867, an experimental oxazolidinone, on the development of experimental mycetomas by N. brasiliensis in BALB/c mice. Methodology/Principal Findings: In order to determine the optimal dose utilized to apply to the animals, we first determined by HPLC the plasma levels using several concentrations of the compounds. Based on these results, we used 10 and 25 mg/kg subcutaneously every 24 hr; DA-7867 was also supplied in the drinking water at a calculated dose of 25 mg/ kg. As a control we utilized linezolid at 25 mg/kg, a compound active in murine and human infections, three times a day. The mice were infected in the right footpad with a young culture of N. brasiliensis HUJEG-1, and one week later we started the application of the antimicrobials for six more weeks. After that we compared the development of lesions in the groups injected with saline solution or with the antimicrobials; the results were analyzed by the variance ANOVA test. DA-7867 was able to reduce the production of lesions at 25 mg/kg, when given either subcutaneously or in the drinking water. Conclusions/Significance: The experimental oxazolidinone DA-7867 is active in vivo against N. brasiliensis, which opens the possibility of using this drug once it is accepted for human application. Since oxazolidinones seem to be active against a wide spectrum of actinobacteria, it is possible they could be used in human cases of mycetoma by other actinomycetales, such as Streptomyces somaliensis, highly prevalent in Sudan, or Actinomadura madurae and A. pelletieri, which are commonly observed in Africa and India.
Food Additives and Contaminants: Part B, 2010
Dioxins in beef were quantified by high resolution gas chromatography coupled to low-resolution m... more Dioxins in beef were quantified by high resolution gas chromatography coupled to low-resolution mass spectrometry (GC/LRMS). The analyses were performed according to the minimum requirements described in the USEPA 1613 method with some minor modifications. Levels found in the samples were in the range 1.02-8.04 pg WHO-TEQ PCDDs/PCDFs g(-1) fat. For comparison purposes, the maximum level allowed by the European Union is 3 pg WHO-TEQ PCDDs/PCDFs g(-1) fat, and some of these samples surpassed the above-mentioned limit and can be considered as contaminated food. The results confirm that a preliminary screening of dioxins in beef can be performed by GC/LRMS. As far as we know, this is the first report of dioxins in beef in Mexico. After the appropriated tests, the applied methodology could be considered as an alternative screening method for the analysis of PCDD/Fs in other food products.
Ciencia …, 2002
Resumen La T-514 es una antracenona dimérica obtenida de plantas del género Karwinskia. Este comp... more Resumen La T-514 es una antracenona dimérica obtenida de plantas del género Karwinskia. Este compuesto posee toxicidad selectiva in vitro hacia células de origen tumoral y es considerado como un potencial agente antineoplásico. En este estudio se ...
Antimicrobial Agents and Chemotherapy, 2008
Analytical Letters, 2008
... la Luz Salazar‐Cavazos a , Noemí H. Waksman de Torres a & Rocío Castro‐Ríos a * p... more ... la Luz Salazar‐Cavazos a , Noemí H. Waksman de Torres a & Rocío Castro‐Ríos a * pages 119-136. ... J. Envir. Anal. Chemi. , 87: 211225. [Taylor & Francis Online], [Web of Science ®] View all references; Reza et al. 200626. Reza, A., Hosseinzadeh, S. and Pawliszyn, J. 2006. ...
BMC Complementary and Alternative Medicine
Current Analytical Chemistry
Resumen Las técnicas empleadas para el control de calidad de la Peroxisomicina A1, fueron mejorad... more Resumen Las técnicas empleadas para el control de calidad de la Peroxisomicina A1, fueron mejoradas aplicando técnicas quimiométricas tanto en CLAR como en espectrofotometría UV-visible. El uso de cromatogramas derivados en CLAR, permitió ...
Planta Medica, Jun 1, 2011