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Papers by Nora Meggyeshazi

Research paper thumbnail of Modulation Effect in Oncothermia

Conference Papers in Medicine, 2013

Conventional hyperthermia is based on the local or systemic heating, which is measured by the rea... more Conventional hyperthermia is based on the local or systemic heating, which is measured by the realized temperature in the process. Oncothermia applies nano-heating, which means high energy absorption in nanoscopic range of the malignant cell-membrane selectively. This high temperature and its consequent stress create special effects: it evolves possibility of chaperone proteins to be expressed on the outer membrane by softening the membrane, and starts various excitations for programmed cell-death of the targeted malignant cell. The process needs special delivery of the energy which selects as desired. A strict 13.56 MHz sinusoidal carrier frequency is amplitude modulated by time-fractal signals. The modulation is far from any sinus or other periodic patterns, it is a 1/f spectrum, having definite templates for its construction. In some personalized cases definite template is used for fractal pattern, which is copied from the actual character of the tumor-pathology or other specialty of the target.

Research paper thumbnail of Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas

Journal of Immunology Research, 2015

show homo-and heterocellular metabolic coupling through connexin 43 (Cx43) gap junctions and supp... more show homo-and heterocellular metabolic coupling through connexin 43 (Cx43) gap junctions and support B cell selection and maturation in germinal centers. In follicular lymphomas B cells escape apoptosis while FDC develop abnormally. Here we tested Cx43 channels in reactive FDC development and follicular lymphomas. In culture, the treatment of FDC-B cell clusters (resembling to "ex vivo" germinal centers) with Gap27 peptide, mimicking the 2nd extracellular loop of Cx43 protein, significantly impaired FDC-B cell cluster formation and cell survival. In untreated cultures of intact clusters, cell proliferation showed a moderate reduction. In tissues, Cx43 protein levels run parallel with the density of FDC both in reactive germinal centers and in malformed follicles of follicular lymphomas and showed strong upregulation in newly generated and/or degrading bi-/multinuclear FDC of rudimentary processes. However, the inverse correlation between Cx43 expression and B cell proliferation seen in reactive germinal centers was not detected in follicular lymphomas. Furthermore, Cx43 levels were not associated with either lymphoma grade or bone marrow involvement. Our results suggest that Cx43 channels are critical in FDC and "ex vivo" germinal center development and in the persistence of FDC in follicular lymphomas but do not affect tumor progression.

Research paper thumbnail of Deep Temperature Measurements in Oncothermia Processes

Conference Papers in Medicine, 2013

Temperature in depth of various model systems was measured, starting with muscle and other phanto... more Temperature in depth of various model systems was measured, starting with muscle and other phantoms. It was shown that the temperature can be selectively increased in the target. In water-protein phantom, the protein coagulation (>60 ∘ C) was observed selectively while the water temperature around it was a little higher than room temperature.

Research paper thumbnail of Report of the Pilot Study Done for the Proposed Investigation on the Possible Synergic Effect between High-Dose Ascorbic Acid Application and Oncothermia Treatment

Conference Papers in Medicine, 2013

According to recent investigations, the parenteral application of ascorbic acid (vitamin C) at hi... more According to recent investigations, the parenteral application of ascorbic acid (vitamin C) at high doses has significant antitumor activity in in vitro assays. The goal of our experiment was to determine the possible potentiating effect of application of high dose pH-neutralized ascorbic acid to the normal oncothermia treatment method. The NMRI mice were inoculated with C26 murine colon carcinoma cell line subcutaneously at both of their femoral regions and were kept till the tumors reached symmetrically 10 mm in diameter. We created four experimental groups, containing 5 male and 5 female animals in each. Both vitamin-C and oncothermia treatments were applied once; ascorbic acid was applied intra-peritoneally. Oncothermia treatment was applied only to the right limb tumor; the other side will be used as internal control. After the treatment, the animals were sacrificed, and all tumors were removed and analyzed histopathologically. Our main question centers on the comparison of the cell destruction ratio of the various applied treatment regimes, and studies the possible synergy or additive cross-potentiating of the methods. The results of this experiment turned out to be controversial, since the ascorbic acid did not change the remission rate of the allografts and showed no synergy with oncothermia.

Research paper thumbnail of Upregulation of heat shock proteins and the promotion of damage-associated molecular pattern signals in a colorectal cancer model by modulated electrohyperthermia

Cell Stress and Chaperones, 2014

In modulated electrohyperthermia (mEHT) the enrichment of electric field and the concomitant heat... more In modulated electrohyperthermia (mEHT) the enrichment of electric field and the concomitant heat can selectively induce cell death in malignant tumors as a result of elevated glycolysis, lactate production (Warburg effect), and reduced electric impedance in cancer compared to normal tissues. Earlier, we showed in HT29 colorectal cancer xenografts that the mEHTprovoked programmed cell death was dominantly caspase independent and driven by apoptosis inducing factor activation. Using this model here, we studied the mEHT-related cell stress 0-, 1-, 4-, 8-, 14-, 24-, 48-, 72-, 120-, 168-and 216-h post-treatment by focusing on damage-associated molecular pattern (DAMP) signals.

Research paper thumbnail of Expression of proliferation markers Ki67, cyclin A, geminin and aurora-kinase A in primary breast carcinomas and corresponding distant metastases

Journal of Clinical Pathology, 2015

Research paper thumbnail of Correlations of Differentially Expressed Gap Junction Connexins Cx26, Cx30, Cx32, Cx43 and Cx46 with Breast Cancer Progression and Prognosis

PLoS ONE, 2014

Background and Aims: Connexins and their cell membrane channels contribute to the control of cell... more Background and Aims: Connexins and their cell membrane channels contribute to the control of cell proliferation and compartmental functions in breast glands and their deregulation is linked to breast carcinogenesis. Our aim was to correlate connexin expression with tumor progression and prognosis in primary breast cancers.

Research paper thumbnail of Modulation Effect in Oncothermia

Conference Papers in Medicine, 2013

Conventional hyperthermia is based on the local or systemic heating, which is measured by the rea... more Conventional hyperthermia is based on the local or systemic heating, which is measured by the realized temperature in the process. Oncothermia applies nano-heating, which means high energy absorption in nanoscopic range of the malignant cell-membrane selectively. This high temperature and its consequent stress create special effects: it evolves possibility of chaperone proteins to be expressed on the outer membrane by softening the membrane, and starts various excitations for programmed cell-death of the targeted malignant cell. The process needs special delivery of the energy which selects as desired. A strict 13.56 MHz sinusoidal carrier frequency is amplitude modulated by time-fractal signals. The modulation is far from any sinus or other periodic patterns, it is a 1/f spectrum, having definite templates for its construction. In some personalized cases definite template is used for fractal pattern, which is copied from the actual character of the tumor-pathology or other specialty of the target.

Research paper thumbnail of Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas

Journal of Immunology Research, 2015

show homo-and heterocellular metabolic coupling through connexin 43 (Cx43) gap junctions and supp... more show homo-and heterocellular metabolic coupling through connexin 43 (Cx43) gap junctions and support B cell selection and maturation in germinal centers. In follicular lymphomas B cells escape apoptosis while FDC develop abnormally. Here we tested Cx43 channels in reactive FDC development and follicular lymphomas. In culture, the treatment of FDC-B cell clusters (resembling to "ex vivo" germinal centers) with Gap27 peptide, mimicking the 2nd extracellular loop of Cx43 protein, significantly impaired FDC-B cell cluster formation and cell survival. In untreated cultures of intact clusters, cell proliferation showed a moderate reduction. In tissues, Cx43 protein levels run parallel with the density of FDC both in reactive germinal centers and in malformed follicles of follicular lymphomas and showed strong upregulation in newly generated and/or degrading bi-/multinuclear FDC of rudimentary processes. However, the inverse correlation between Cx43 expression and B cell proliferation seen in reactive germinal centers was not detected in follicular lymphomas. Furthermore, Cx43 levels were not associated with either lymphoma grade or bone marrow involvement. Our results suggest that Cx43 channels are critical in FDC and "ex vivo" germinal center development and in the persistence of FDC in follicular lymphomas but do not affect tumor progression.

Research paper thumbnail of Deep Temperature Measurements in Oncothermia Processes

Conference Papers in Medicine, 2013

Temperature in depth of various model systems was measured, starting with muscle and other phanto... more Temperature in depth of various model systems was measured, starting with muscle and other phantoms. It was shown that the temperature can be selectively increased in the target. In water-protein phantom, the protein coagulation (>60 ∘ C) was observed selectively while the water temperature around it was a little higher than room temperature.

Research paper thumbnail of Report of the Pilot Study Done for the Proposed Investigation on the Possible Synergic Effect between High-Dose Ascorbic Acid Application and Oncothermia Treatment

Conference Papers in Medicine, 2013

According to recent investigations, the parenteral application of ascorbic acid (vitamin C) at hi... more According to recent investigations, the parenteral application of ascorbic acid (vitamin C) at high doses has significant antitumor activity in in vitro assays. The goal of our experiment was to determine the possible potentiating effect of application of high dose pH-neutralized ascorbic acid to the normal oncothermia treatment method. The NMRI mice were inoculated with C26 murine colon carcinoma cell line subcutaneously at both of their femoral regions and were kept till the tumors reached symmetrically 10 mm in diameter. We created four experimental groups, containing 5 male and 5 female animals in each. Both vitamin-C and oncothermia treatments were applied once; ascorbic acid was applied intra-peritoneally. Oncothermia treatment was applied only to the right limb tumor; the other side will be used as internal control. After the treatment, the animals were sacrificed, and all tumors were removed and analyzed histopathologically. Our main question centers on the comparison of the cell destruction ratio of the various applied treatment regimes, and studies the possible synergy or additive cross-potentiating of the methods. The results of this experiment turned out to be controversial, since the ascorbic acid did not change the remission rate of the allografts and showed no synergy with oncothermia.

Research paper thumbnail of Upregulation of heat shock proteins and the promotion of damage-associated molecular pattern signals in a colorectal cancer model by modulated electrohyperthermia

Cell Stress and Chaperones, 2014

In modulated electrohyperthermia (mEHT) the enrichment of electric field and the concomitant heat... more In modulated electrohyperthermia (mEHT) the enrichment of electric field and the concomitant heat can selectively induce cell death in malignant tumors as a result of elevated glycolysis, lactate production (Warburg effect), and reduced electric impedance in cancer compared to normal tissues. Earlier, we showed in HT29 colorectal cancer xenografts that the mEHTprovoked programmed cell death was dominantly caspase independent and driven by apoptosis inducing factor activation. Using this model here, we studied the mEHT-related cell stress 0-, 1-, 4-, 8-, 14-, 24-, 48-, 72-, 120-, 168-and 216-h post-treatment by focusing on damage-associated molecular pattern (DAMP) signals.

Research paper thumbnail of Expression of proliferation markers Ki67, cyclin A, geminin and aurora-kinase A in primary breast carcinomas and corresponding distant metastases

Journal of Clinical Pathology, 2015

Research paper thumbnail of Correlations of Differentially Expressed Gap Junction Connexins Cx26, Cx30, Cx32, Cx43 and Cx46 with Breast Cancer Progression and Prognosis

PLoS ONE, 2014

Background and Aims: Connexins and their cell membrane channels contribute to the control of cell... more Background and Aims: Connexins and their cell membrane channels contribute to the control of cell proliferation and compartmental functions in breast glands and their deregulation is linked to breast carcinogenesis. Our aim was to correlate connexin expression with tumor progression and prognosis in primary breast cancers.