Noraziyah Abd Aziz Shamsudin - Academia.edu (original) (raw)

Papers by Noraziyah Abd Aziz Shamsudin

Data in Brief, Jan 31, 2024

Frontiers in Plant Science, Oct 16, 2017

tropical life sciences research, Jul 15, 2022

Plant Science Today

Rice is a staple food and one of the most crucial crops globally, providing sustenance for more t... more Rice is a staple food and one of the most crucial crops globally, providing sustenance for more than half of the world's population. Climate change has a crucial impact on the agricultural sector, particularly rice cultivation, due to the increase in abiotic stress incidences. Salinity is one of the most severe abiotic stresses on rice production globally. Salt stress significantly reduces growth performance, affecting various metabolic and physiological processes in rice. Submergence is another type of abiotic stress affecting rice growth and yield. Recently, a newly emerged abiotic stress called saline submergence may also jeopardize rice production. Seawater intrusion into rice fields located nearby coastal areas may cause saline flash floods, especially during monsoon season. Rice cultivated in coastal areas is prone to saline-submergence stress, leading to a significantly lower yield. Although Sub1 and Saltol QTLs are widely used in developing rice cultivars with submergenc...

Plant Science Today

Salinity is one of the most serious issues in rice cultivation and production. Salt stress signif... more Salinity is one of the most serious issues in rice cultivation and production. Salt stress significantly reduced seedling growth performance of rice. This research was conducted to study the effects of vegetative stage salinity stress on morphological, biochemical, molecular and genetic variation of 12 rice genotypes as well as 2 check varieties, MR297 (susceptible) and Pokkali (tolerant). The experiment was arranged in a split-plot design with 3 replications. Normal freshwater at 0 dS m-1 (L1), saline water at 6 dS m-1 (L2) and saline water at 12 dS m-1 (L3) were the main plot and rice genotypes were the sub-plot. In general, morphological and biochemical traits of all genotypes showed an overall reduction of about 47.41% in L3 as compared to L1 except for the tolerant check, Pokkali. The genetics and correlation analysis indicated that plant height, leaf size and standard evaluation system (SES) score might be used as a selection criterion in developing salt tolerant rice. The mul...

Euphytica, 2021

Rice blast, caused by Magnaporthe oryzae , is a destructive disease. A total of 23 rice genotypes... more Rice blast, caused by Magnaporthe oryzae , is a destructive disease. A total of 23 rice genotypes, were screened using a set of 51 simple sequence repeats SSR/ InDel markers and 51 single nucleotide polymorphic (SNP) markers to evaluate the sequence variation. Six newly identified Malaysian isolates of M. oryzae were used for blast screening. The resistant genotypes, consisting of Pongsu Seribu 2, O. rufipogon accessions, Tetep and IR64 had a resistant disease score of 2/3 showing minimal symptoms of blast. In contrast, Mahsuri and MR264 were the most susceptible and had a disease score of 5. In total, 11 sequence variations and two putative haplotype blocks, were observed in the SNP data representing Pi54 and Pita . The SNPs for Pita and Pi54 revealed the presence of resistant alleles that generated reproducible and aggressive calls based on the Sequenom data, but when screened with SSR and INDel markers showed poor correspondence to phenotype effects. Therefore, SNPs for Pi54 and Pita were found to be the most reliable for marker-assisted selection. The blast-resistant alleles identified in this study indicate that they are involved in the defence response against diverse isolates and play a role in putative broad spectrum resistance.

Sains Malaysiana

Global climate change has not only caused a significant rise in the average temperature around th... more Global climate change has not only caused a significant rise in the average temperature around the world but has also threatened crop productivity and food security. Heat stress disrupts various plant physiological and biochemical processes, such as inhibition of growth and development, reduction of photosynthesis rate and nutrient uptake, consequently causing yield losses. The destructive effects of heat stress are expected to worsen in the coming years. Thus, it has become imperative to understand how vegetables respond and adapt to heat stress in order to improve their heat tolerance ability. Various approaches have been adopted to enhance heat stress tolerance in vegetables, including modifying cultural practices and crop improvements through several breeding methods. This review gives comprehensive and up-to-date information on the effects of heat stress on vegetables; and existing as well as emerging methods adopted to enhance heat tolerance in vegetables. It also provides a b...

IOP Conference Series: Earth and Environmental Science

A new high-yielding mutant rice cultivar, IS21, has been released by the Malaysian Nuclear Agency... more A new high-yielding mutant rice cultivar, IS21, has been released by the Malaysian Nuclear Agency (MNA) in 2021. This cultivar matures in 105-108 days after transplanting. Since there is a need to reduce days to maturity (DTM) in most Malaysian rice mega-varieties to minimise the impacts of abiotic and biotic stresses, crossing IS21 with early maturing rice genotypes could be an efficient strategy to address these issues. Preliminary screening has successfully identified two early maturing and shorter plant statue rice genotypes, MR220CL2 and UKMRC16. Therefore, these rice genotypes were crossed to IS21 to generate two F1 populations. These F1 populations (IS21 × MR220CL2 and IS21 × UKMRC16) were planted in the MNA glasshouse with parental lines from December 2021 to March 2022. The agro-morphological data for F1 populations and parental lines were recorded and analysed using the RStudio software package. The putative F1 progenies were confirmed using two polymorphic simple sequence...

Plant Science Today

This study investigates the submergence tolerance level of 14 rice genotypes by morpho-physiologi... more This study investigates the submergence tolerance level of 14 rice genotypes by morpho-physiological and molecular analyses of Sub1 alleles. IR64-Sub1 was used as a submergence tolerant check. The submergence screenings were conducted by submerging 14-days-old seedlings in water tanks for 14 days while molecular analysis was conducted using 14 Sub1 linked markers. The results showed that 5 tested genotypes, namely Inpari 48 Blas, Mundam Putiah, Batang Piaman, Banang Pulau and Pulau Sijunjung, recorded high survival rates (SR) of 80% to 100% under submergence stress. PCR-based identification of the Sub1 alleles confirmed that several tested genotypes carry Sub1A and Sub1C, but not all were expressed in their phenotypic performance towards submergence. IR64-Sub1 and Batang Piaman not only showed well-adaptation towards submergence by maintaining lower elongation (<20%) and less chlorophyll content change (TCC) (<30%) but were also equipped with the Sub1A allele. These genotypes ...

Food Research

Bitter gourd is one of the potential vegetables that has been clinically proven with several uses... more Bitter gourd is one of the potential vegetables that has been clinically proven with several uses due to its medicinal advantages. However, it is less popular than other vegetable crops. Hence, this study was conducted to obtain detailed information on important agronomic traits and determine the pattern of inheritance of morphological traits in bitter gourd. Four F1 populations, P105 × P11, P11 × P92, P92 × P13 and P13 × P92 have been successfully generated from the hybridisation of four genotypes parental lines (P11, P13, P92 and P105). The result of analysis of variance showed that the parents and the F1 populations were significantly different (p < 0.01) in terms of vine length at 90 days after sowing, days of the first harvest after flowering, fruit number, fruit weight, fruit length, fruit girth, flesh thickness and total yield per plant. It also exhibited significantly different (p < 0.05) on days of to first male and female flower appearances. F1 progeny P11 × P92 reco...

Malaysian Applied Biology

Weedy rice or locally known as ‘padi angin’ has been infesting rice granary areas in Malaysia for... more Weedy rice or locally known as ‘padi angin’ has been infesting rice granary areas in Malaysia for the last 20 years. The outbreak of weedy rice in the country started during the late 1980s in Projek Barat Laut Selangor (PBLS) due to the adoption of direct-seeding practices. Weedy rice can be characterized by its early maturing and easy-shattering traits. The objective of this study was to determine the morphological variations of weedy rice in selected granary areas located in Peninsular Malaysia. A total of 110 weedy rice accessions were collected from nine locations in three granary areas in Peninsular Malaysia (the Western, Eastern, & Northern regions). The collected weedy rice accessions were planted under greenhouse conditions to study their morphological features. Results of the present study indicated that 73.6% of weedy rice accessions were awnless while the remaining 26.4% produced awn. Selected traits such as plant height, flag leaf length, culm length, number of tillers, ...

Malaysian Applied Biology

Various varieties have been developed in Malaysia, mainly to improve rice response to environment... more Various varieties have been developed in Malaysia, mainly to improve rice response to environmental changes, pests, and diseases, as well as to increase rice productivity under stressful conditions. Despite being semi-aquatic plants, rice is intolerant to complete submergence for a long period. This study was conducted to evaluate the response of seven Malaysian rice varieties at the vegetative stage under submergence stress. Two-week-old rice seedlings were submerged for 14 days, and the changes in plant height, chlorophyll content, and soluble sugar content were determined. The survival percentage of these varieties was observed after 14 days of de-submergence, where UKMRC2 and MR220CL possessed high survivability (90% & 60%, respectively). After submergence, all varieties showed height increment and reduced chlorophyll and soluble sugar contents. Based on our analyses, UKMRC2 performed better than other varieties, although slightly less than IR64-Sub1. It was confirmed that UKMRC...

Life

The introduction of superior grain corn genotypes with high and stable yield (YLD) in most enviro... more The introduction of superior grain corn genotypes with high and stable yield (YLD) in most environments is important to increase local production and reduce dependency on imported grain corn. In this study, days to tasseling (DT), plant height, and YLD of 11 grain corn genotypes were observed in 10 environments to evaluate the effects of genotype (G), environment (E), and genotype by environment interactions (GEI) using GGE analysis and the stability of genotypes using stability parameters. In each location, grain corn genotypes were arranged in three replications using a randomized complete block design. An analysis of variance showed that all three traits were highly significant toward G and E factors, whereas GEI showed that only DT and YLD were highly significant. Genotype V14 produced the highest YLD of 10,354 kg/ha, followed by V4 (10,114 kg/ha) and V2 (9797.74 kg/ha). These three genotypes also dominated in seven out of 10 tested environments. With regard to stability ranking...

Agronomy

Drought is one of the major limitations to rice productivity worldwide. The present study compare... more Drought is one of the major limitations to rice productivity worldwide. The present study compared variation in seventeen rice genotypes of Egyptian origin for morpho-physiological traits to identify the best genotypes with combination of adaptive traits under water-limited condition (DS). The DS reduced days to heading (DTH), plant height (PH), flag leaf angle (FLA), flag leaf area (FLAR), chlorophyll content (CHC), relative water content (RWC), grain yield (GY), and its components. Among genotypes, Hybrid 2 expressed the highest GY, panicle length (PL), number of tillers (NT), panicles per plant (NPP), and harvest index (HI) with maximum spikelet sterility (SS) under non-stress condition (NS), while the same genotype expressed ≈ 41% yield reduction under DS. The genotype Giza 179 had earlier DTH, higher and stable GY, FLAR, and yield component traits such as NPP, PW, and HI across the water regimes with least yield reduction (30.5%) under DS. The GY and FLAR, RWC, PL, NT, NPP, PW,...

Agronomy

MR219 transgenic rice line which overexpressed an auxin-binding protein (ABP57) and its wild-type... more MR219 transgenic rice line which overexpressed an auxin-binding protein (ABP57) and its wild-type cultivar, MR219, were screened under well-watered (WW) and drought stress (DS) conditions at the early reproductive stage. This study was conducted with the standard planting distance and under a normal environment to assess the yield advantages based on the field conditions. The aim of this study was to understand the response of these rice genotypes towards DS at morpho-physiological, biochemical, and agronomical levels. It was found that the DS had affected all these levels of the genotypes studied; however, the transgenic plant showed a higher number of tillers, flag leaf area, biomass, relative water content, total chlorophyll content, and antioxidative defense mechanism than the MR219 under DS. Compared to its wild-type, the transgenic plant showed an increased leaf photosynthetic rate by 7% under WW and 11% under DS. The transgenic plant also showed higher yields than MR219 under...

Sains Malaysiana, 2021

Seed priming could be promoted as a potential alternative in alleviating drought stress challenge... more Seed priming could be promoted as a potential alternative in alleviating drought stress challenges in rice cultivation. The present study was conducted as an attempt to verify potential performance of seed priming in improving seedling growth and harvestable grain yield of rice under reproductive stage drought stress (RS). Seed treatments involved were non-primed seeds as control (T1), hydro-primed (T2) and osmo-primed at -1.0 mPa with polyethylene glycol (PEG6000) (T3). Reproductive stage drought stress (RS) was imposed at soil water potential lower than -60 kPa. The well-watered plants served as control of the experiment. In general, seedling growth of T3 was better than T2 and T1 for all growth parameters in both planting seasons. Yield components were significantly lower in RS as compared to well-watered treatment (WW). The agronomic performance of primed seeds in T2 and T3 were not significantly different with T1 under both RS and WW for both planting seasons. As a conclusion, ...

Universiti Sultan Zainal Abidin, Mar 2, 2021

Salinity stress would significantly reduce seedling growth performance of rice. In addition, sali... more Salinity stress would significantly reduce seedling growth performance of rice. In addition, salinity also affects physiological and metabolic process mainly the osmotic and ionic balance of the cells. Hence, the present study was conducted to evaluate morpho-physiological and biochemical response of selected rice genotypes to salinity stress at seedling stage. Twelve rice genotypes were used in the pot-trial experiment including two checks namely Pokkali (tolerant) and MR297 (susceptible). The experiment was conducted in a split plot design with three replications. Three salinity levels involved were L1 (normal fresh water), L2 (saline water at 12 dSm-1), and L3 (saline water at 24 dSm-1) as the main plot while rice genotypes as the sub-plot. Salinity stress was imposed for 14 days starting from 21 days after sowing. In overall, L3 salinity stress significantly reduced 47.41% of all seedling growth attributes for all genotypes except for Pokkali (V11) as compared to control condition. Meanwhile, Haiboq (V9) and Basmati 370 (V3) recorded significantly similar response as the MR297 (V10). The trend of chlorophyll content reduction could be seen in all genotypes under L2 and L3 salinity stress with average 77.72% reduced over control condition. In contrast, proline content was increased over 7 folds in all genotypes as level of salinity increases except for V11. Proline may function as a signal metabolites thus higher proline content indicates that the plant is under stress. In conclusion, chlorophyll and proline content may be used as indicators of sensitivity to salinity stress in rice cultivars along with the morphological growth responses.

Data in Brief, Jan 31, 2024

Frontiers in Plant Science, Oct 16, 2017

tropical life sciences research, Jul 15, 2022

Plant Science Today

Rice is a staple food and one of the most crucial crops globally, providing sustenance for more t... more Rice is a staple food and one of the most crucial crops globally, providing sustenance for more than half of the world's population. Climate change has a crucial impact on the agricultural sector, particularly rice cultivation, due to the increase in abiotic stress incidences. Salinity is one of the most severe abiotic stresses on rice production globally. Salt stress significantly reduces growth performance, affecting various metabolic and physiological processes in rice. Submergence is another type of abiotic stress affecting rice growth and yield. Recently, a newly emerged abiotic stress called saline submergence may also jeopardize rice production. Seawater intrusion into rice fields located nearby coastal areas may cause saline flash floods, especially during monsoon season. Rice cultivated in coastal areas is prone to saline-submergence stress, leading to a significantly lower yield. Although Sub1 and Saltol QTLs are widely used in developing rice cultivars with submergenc...

Plant Science Today

Salinity is one of the most serious issues in rice cultivation and production. Salt stress signif... more Salinity is one of the most serious issues in rice cultivation and production. Salt stress significantly reduced seedling growth performance of rice. This research was conducted to study the effects of vegetative stage salinity stress on morphological, biochemical, molecular and genetic variation of 12 rice genotypes as well as 2 check varieties, MR297 (susceptible) and Pokkali (tolerant). The experiment was arranged in a split-plot design with 3 replications. Normal freshwater at 0 dS m-1 (L1), saline water at 6 dS m-1 (L2) and saline water at 12 dS m-1 (L3) were the main plot and rice genotypes were the sub-plot. In general, morphological and biochemical traits of all genotypes showed an overall reduction of about 47.41% in L3 as compared to L1 except for the tolerant check, Pokkali. The genetics and correlation analysis indicated that plant height, leaf size and standard evaluation system (SES) score might be used as a selection criterion in developing salt tolerant rice. The mul...

Euphytica, 2021

Rice blast, caused by Magnaporthe oryzae , is a destructive disease. A total of 23 rice genotypes... more Rice blast, caused by Magnaporthe oryzae , is a destructive disease. A total of 23 rice genotypes, were screened using a set of 51 simple sequence repeats SSR/ InDel markers and 51 single nucleotide polymorphic (SNP) markers to evaluate the sequence variation. Six newly identified Malaysian isolates of M. oryzae were used for blast screening. The resistant genotypes, consisting of Pongsu Seribu 2, O. rufipogon accessions, Tetep and IR64 had a resistant disease score of 2/3 showing minimal symptoms of blast. In contrast, Mahsuri and MR264 were the most susceptible and had a disease score of 5. In total, 11 sequence variations and two putative haplotype blocks, were observed in the SNP data representing Pi54 and Pita . The SNPs for Pita and Pi54 revealed the presence of resistant alleles that generated reproducible and aggressive calls based on the Sequenom data, but when screened with SSR and INDel markers showed poor correspondence to phenotype effects. Therefore, SNPs for Pi54 and Pita were found to be the most reliable for marker-assisted selection. The blast-resistant alleles identified in this study indicate that they are involved in the defence response against diverse isolates and play a role in putative broad spectrum resistance.

Sains Malaysiana

Global climate change has not only caused a significant rise in the average temperature around th... more Global climate change has not only caused a significant rise in the average temperature around the world but has also threatened crop productivity and food security. Heat stress disrupts various plant physiological and biochemical processes, such as inhibition of growth and development, reduction of photosynthesis rate and nutrient uptake, consequently causing yield losses. The destructive effects of heat stress are expected to worsen in the coming years. Thus, it has become imperative to understand how vegetables respond and adapt to heat stress in order to improve their heat tolerance ability. Various approaches have been adopted to enhance heat stress tolerance in vegetables, including modifying cultural practices and crop improvements through several breeding methods. This review gives comprehensive and up-to-date information on the effects of heat stress on vegetables; and existing as well as emerging methods adopted to enhance heat tolerance in vegetables. It also provides a b...

IOP Conference Series: Earth and Environmental Science

A new high-yielding mutant rice cultivar, IS21, has been released by the Malaysian Nuclear Agency... more A new high-yielding mutant rice cultivar, IS21, has been released by the Malaysian Nuclear Agency (MNA) in 2021. This cultivar matures in 105-108 days after transplanting. Since there is a need to reduce days to maturity (DTM) in most Malaysian rice mega-varieties to minimise the impacts of abiotic and biotic stresses, crossing IS21 with early maturing rice genotypes could be an efficient strategy to address these issues. Preliminary screening has successfully identified two early maturing and shorter plant statue rice genotypes, MR220CL2 and UKMRC16. Therefore, these rice genotypes were crossed to IS21 to generate two F1 populations. These F1 populations (IS21 × MR220CL2 and IS21 × UKMRC16) were planted in the MNA glasshouse with parental lines from December 2021 to March 2022. The agro-morphological data for F1 populations and parental lines were recorded and analysed using the RStudio software package. The putative F1 progenies were confirmed using two polymorphic simple sequence...

Plant Science Today

This study investigates the submergence tolerance level of 14 rice genotypes by morpho-physiologi... more This study investigates the submergence tolerance level of 14 rice genotypes by morpho-physiological and molecular analyses of Sub1 alleles. IR64-Sub1 was used as a submergence tolerant check. The submergence screenings were conducted by submerging 14-days-old seedlings in water tanks for 14 days while molecular analysis was conducted using 14 Sub1 linked markers. The results showed that 5 tested genotypes, namely Inpari 48 Blas, Mundam Putiah, Batang Piaman, Banang Pulau and Pulau Sijunjung, recorded high survival rates (SR) of 80% to 100% under submergence stress. PCR-based identification of the Sub1 alleles confirmed that several tested genotypes carry Sub1A and Sub1C, but not all were expressed in their phenotypic performance towards submergence. IR64-Sub1 and Batang Piaman not only showed well-adaptation towards submergence by maintaining lower elongation (<20%) and less chlorophyll content change (TCC) (<30%) but were also equipped with the Sub1A allele. These genotypes ...

Food Research

Bitter gourd is one of the potential vegetables that has been clinically proven with several uses... more Bitter gourd is one of the potential vegetables that has been clinically proven with several uses due to its medicinal advantages. However, it is less popular than other vegetable crops. Hence, this study was conducted to obtain detailed information on important agronomic traits and determine the pattern of inheritance of morphological traits in bitter gourd. Four F1 populations, P105 × P11, P11 × P92, P92 × P13 and P13 × P92 have been successfully generated from the hybridisation of four genotypes parental lines (P11, P13, P92 and P105). The result of analysis of variance showed that the parents and the F1 populations were significantly different (p < 0.01) in terms of vine length at 90 days after sowing, days of the first harvest after flowering, fruit number, fruit weight, fruit length, fruit girth, flesh thickness and total yield per plant. It also exhibited significantly different (p < 0.05) on days of to first male and female flower appearances. F1 progeny P11 × P92 reco...

Malaysian Applied Biology

Weedy rice or locally known as ‘padi angin’ has been infesting rice granary areas in Malaysia for... more Weedy rice or locally known as ‘padi angin’ has been infesting rice granary areas in Malaysia for the last 20 years. The outbreak of weedy rice in the country started during the late 1980s in Projek Barat Laut Selangor (PBLS) due to the adoption of direct-seeding practices. Weedy rice can be characterized by its early maturing and easy-shattering traits. The objective of this study was to determine the morphological variations of weedy rice in selected granary areas located in Peninsular Malaysia. A total of 110 weedy rice accessions were collected from nine locations in three granary areas in Peninsular Malaysia (the Western, Eastern, & Northern regions). The collected weedy rice accessions were planted under greenhouse conditions to study their morphological features. Results of the present study indicated that 73.6% of weedy rice accessions were awnless while the remaining 26.4% produced awn. Selected traits such as plant height, flag leaf length, culm length, number of tillers, ...

Malaysian Applied Biology

Various varieties have been developed in Malaysia, mainly to improve rice response to environment... more Various varieties have been developed in Malaysia, mainly to improve rice response to environmental changes, pests, and diseases, as well as to increase rice productivity under stressful conditions. Despite being semi-aquatic plants, rice is intolerant to complete submergence for a long period. This study was conducted to evaluate the response of seven Malaysian rice varieties at the vegetative stage under submergence stress. Two-week-old rice seedlings were submerged for 14 days, and the changes in plant height, chlorophyll content, and soluble sugar content were determined. The survival percentage of these varieties was observed after 14 days of de-submergence, where UKMRC2 and MR220CL possessed high survivability (90% & 60%, respectively). After submergence, all varieties showed height increment and reduced chlorophyll and soluble sugar contents. Based on our analyses, UKMRC2 performed better than other varieties, although slightly less than IR64-Sub1. It was confirmed that UKMRC...

Life

The introduction of superior grain corn genotypes with high and stable yield (YLD) in most enviro... more The introduction of superior grain corn genotypes with high and stable yield (YLD) in most environments is important to increase local production and reduce dependency on imported grain corn. In this study, days to tasseling (DT), plant height, and YLD of 11 grain corn genotypes were observed in 10 environments to evaluate the effects of genotype (G), environment (E), and genotype by environment interactions (GEI) using GGE analysis and the stability of genotypes using stability parameters. In each location, grain corn genotypes were arranged in three replications using a randomized complete block design. An analysis of variance showed that all three traits were highly significant toward G and E factors, whereas GEI showed that only DT and YLD were highly significant. Genotype V14 produced the highest YLD of 10,354 kg/ha, followed by V4 (10,114 kg/ha) and V2 (9797.74 kg/ha). These three genotypes also dominated in seven out of 10 tested environments. With regard to stability ranking...

Agronomy

Drought is one of the major limitations to rice productivity worldwide. The present study compare... more Drought is one of the major limitations to rice productivity worldwide. The present study compared variation in seventeen rice genotypes of Egyptian origin for morpho-physiological traits to identify the best genotypes with combination of adaptive traits under water-limited condition (DS). The DS reduced days to heading (DTH), plant height (PH), flag leaf angle (FLA), flag leaf area (FLAR), chlorophyll content (CHC), relative water content (RWC), grain yield (GY), and its components. Among genotypes, Hybrid 2 expressed the highest GY, panicle length (PL), number of tillers (NT), panicles per plant (NPP), and harvest index (HI) with maximum spikelet sterility (SS) under non-stress condition (NS), while the same genotype expressed ≈ 41% yield reduction under DS. The genotype Giza 179 had earlier DTH, higher and stable GY, FLAR, and yield component traits such as NPP, PW, and HI across the water regimes with least yield reduction (30.5%) under DS. The GY and FLAR, RWC, PL, NT, NPP, PW,...

Agronomy

MR219 transgenic rice line which overexpressed an auxin-binding protein (ABP57) and its wild-type... more MR219 transgenic rice line which overexpressed an auxin-binding protein (ABP57) and its wild-type cultivar, MR219, were screened under well-watered (WW) and drought stress (DS) conditions at the early reproductive stage. This study was conducted with the standard planting distance and under a normal environment to assess the yield advantages based on the field conditions. The aim of this study was to understand the response of these rice genotypes towards DS at morpho-physiological, biochemical, and agronomical levels. It was found that the DS had affected all these levels of the genotypes studied; however, the transgenic plant showed a higher number of tillers, flag leaf area, biomass, relative water content, total chlorophyll content, and antioxidative defense mechanism than the MR219 under DS. Compared to its wild-type, the transgenic plant showed an increased leaf photosynthetic rate by 7% under WW and 11% under DS. The transgenic plant also showed higher yields than MR219 under...

Sains Malaysiana, 2021

Seed priming could be promoted as a potential alternative in alleviating drought stress challenge... more Seed priming could be promoted as a potential alternative in alleviating drought stress challenges in rice cultivation. The present study was conducted as an attempt to verify potential performance of seed priming in improving seedling growth and harvestable grain yield of rice under reproductive stage drought stress (RS). Seed treatments involved were non-primed seeds as control (T1), hydro-primed (T2) and osmo-primed at -1.0 mPa with polyethylene glycol (PEG6000) (T3). Reproductive stage drought stress (RS) was imposed at soil water potential lower than -60 kPa. The well-watered plants served as control of the experiment. In general, seedling growth of T3 was better than T2 and T1 for all growth parameters in both planting seasons. Yield components were significantly lower in RS as compared to well-watered treatment (WW). The agronomic performance of primed seeds in T2 and T3 were not significantly different with T1 under both RS and WW for both planting seasons. As a conclusion, ...

Universiti Sultan Zainal Abidin, Mar 2, 2021

Salinity stress would significantly reduce seedling growth performance of rice. In addition, sali... more Salinity stress would significantly reduce seedling growth performance of rice. In addition, salinity also affects physiological and metabolic process mainly the osmotic and ionic balance of the cells. Hence, the present study was conducted to evaluate morpho-physiological and biochemical response of selected rice genotypes to salinity stress at seedling stage. Twelve rice genotypes were used in the pot-trial experiment including two checks namely Pokkali (tolerant) and MR297 (susceptible). The experiment was conducted in a split plot design with three replications. Three salinity levels involved were L1 (normal fresh water), L2 (saline water at 12 dSm-1), and L3 (saline water at 24 dSm-1) as the main plot while rice genotypes as the sub-plot. Salinity stress was imposed for 14 days starting from 21 days after sowing. In overall, L3 salinity stress significantly reduced 47.41% of all seedling growth attributes for all genotypes except for Pokkali (V11) as compared to control condition. Meanwhile, Haiboq (V9) and Basmati 370 (V3) recorded significantly similar response as the MR297 (V10). The trend of chlorophyll content reduction could be seen in all genotypes under L2 and L3 salinity stress with average 77.72% reduced over control condition. In contrast, proline content was increased over 7 folds in all genotypes as level of salinity increases except for V11. Proline may function as a signal metabolites thus higher proline content indicates that the plant is under stress. In conclusion, chlorophyll and proline content may be used as indicators of sensitivity to salinity stress in rice cultivars along with the morphological growth responses.

Background: Three drought yield QTLs, qDTY 2.2 , qDTY 3.1, and qDTY 12.1 with consistent effect o... more Background: Three drought yield QTLs, qDTY 2.2 , qDTY 3.1, and qDTY 12.1 with consistent effect on grain yield under reproductive stage drought stress were pyramided through marker assisted breeding with the objective of improving the grain yield of the elite Malaysian rice cultivar MR219 under reproductive stage drought stress. Foreground selection using QTL specific markers, recombinant selection using flanking markers, and background selection were performed. BC 1 F 3-derived lines with different combinations of qDTY 2.2 , qDTY 3.1, and qDTY 12.1 were evaluated under both reproductive stage drought stress and non-stress during the dry seasons of 2013 and 2014 at IRRI. Results: The grain yield reductions in the stress trials compared to non-stress trials ranged from 79 to 93 %. In the stress trials, delay in days to flowering and reduction in plant height were observed. In both seasons, MR219 did not produce any yield under stress, however it produced a yield of 5917 kg ha −1 during the 2013 dry season and 8319 kg ha −1 during the 2014 dry season under non-stress. Selected introgressed lines gave a yield advantage of 903 to 2500 kg ha −1 over MR219 under reproductive stage drought stress and a yield of more than 6900 kg ha −1 under non-stress during the 2014 dry season. Among lines with single qDTY, lines carrying qDTY 2.2 provided a higher yield advantage under reproductive stage drought stress in the MR219 background. Two-qDTY combinations (qDTY 3.1+ qDTY 2.2 and qDTY 3.1+ qDTY 12.1) performed better than lines with three qDTY combinations, indicating the absence of positive interactions between the three qDTYs.