Nosa Terry Omorodion - Academia.edu (original) (raw)

Papers by Nosa Terry Omorodion

MOJ biology and medicine, Jun 29, 2017

Immunohisto chemistry is used to characterize various surface and intracellular proteins from cel... more Immunohisto chemistry is used to characterize various surface and intracellular proteins from cells of all tissues. Individual markers and more often panels of various marker proteins can be used to distinguish carcinoma from sarcoma or lymphoma, mesothelioma or melanoma, characterize various tumor subtypes, confirm tissue of origin, help distinguish metastatic from primary tumor and provide additional information about tumors, which may be important for staging, prognosis, predicting response to therapy or evaluating for residual tumor post treatment. The most common panel is one used for breast cancer patients to determine hormone receptor expression and other markers that predict response to certain therapies and determine risk of recurrence to assist in treatment decisions such as surgery, radiation therapy and chemotherapy. Common breast cancer prognostic and therapeutic markers used include: ER, HER2, Ki-67 and PR. Aim of the study is to demonstrate immunohistochemistry markers on samples. Objective: To confirm the superiority of IHC over conventional H$E. The trend shows that 58.5% of the patients were HER-2 and Ki-67 negative. This is an indication that there is likelihood for the patient to survive through effective treatment. The analysis indicated that 1(2.4%) patients were (ER, PR) positive and Ki-67 negative. It also shows that 10(24.3%) patients were (ER, PR) negative and Ki-67 positive. Findings also show that 2(4.9%) patients were (ER, PR) positive and Ki-67 positive. Finally, it shows that 19(46.3%) patients were (ER, PR) negative and Ki-67 negative.

Acta Scientific Pharmaceutical Sciences, 2020

Pausinystalia johimbe is a common evergreen tree which belongs to the family of the Rubiacease, t... more Pausinystalia johimbe is a common evergreen tree which belongs to the family of the Rubiacease, this plant is known to be found amongst the south, west and central African region in then main forest and jungles of cameroun, Nigeria, Gabon and equatorial guinea. This tree is known to grow up to 30 meters high and has the capacity to sprout out heavily having brown colored backs which is usually spotted with lichen [3]. The stems and branch grow extensively and the have elliptical leaves. This plant is known amongst the locals as Burantashi, it functions as a sexual enhancer

NIPES Journal of Science and Technology Research, 2020

The search for alternative and renewable fuels has increased globally in recent times owing to th... more The search for alternative and renewable fuels has increased globally in recent times owing to the increasing pressure to reduce greenhouse gas. In this work, biodiesel was produced from soya bean oil via transesterification with methanol in the presence of catalyst (sodium hydroxide). The biodiesel was purified adopting the wet purification method. The clean biodiesel was characterised by determining some physico-chemical parameters such as density, flash point, viscosity, free fatty acid (FFA), cloud point, pour point, saponification value and cetane number. The results obtained show that most of the parameters tested fell within the standard ranges as specified by the ASTM. D6751 and EN 14214. The transesterification of soya bean oil has significantly reduced the viscosity, acid value and flash point of the oil by 66.4%, 71.6% and 55.7% respectively. The cetane number was however on the higher range of the standard values; this is an indication that the biodiesel has a good fuel ignition property. It is therefore ascertained that the biodiesel from soya bean oil is a viable alternative to petroleum diesel, since all the quality parameters are within the standard values.

Nigerian Journal of Pharmaceutical and Applied Science Research, Jul 23, 2021

The Journal of Infection in Developing Countries, 2022

Medical laboratory personnel encounter diverse health and workplace-related hazards leading to se... more Medical laboratory personnel encounter diverse health and workplace-related hazards leading to severe health challenges including the ravaging SARS-CoV-2 infection, which is the causal agent of COVID-19. It was first announced in Wuhan, China in December 2019 but started to spread globally by late January 2020. COVID-19 pandemic and subsequent global spreading poses additional danger to healthcare personnel particularly the laboratorians. Other health practitioners may engage patients by observing social / physical distancing, but how laboratory staff observe or apply same rule to infectious samples remain a notable question. Activities of laboratorians result in repeated exposure at close interactions to patient’s samples including SARS-CoV-2 infected specimens, which make them susceptible to COVID-19. Therefore, it is imperative to review mitigating measures in restraining possible exposure and spreading of SARS-CoV-2 in the best interest of laboratory staff and pathologists. It i...

American Journal of Biomedical Sciences, 2018

Parasite is an organism living in, with, or on another organism in parasitism. Parasites are crea... more Parasite is an organism living in, with, or on another organism in parasitism. Parasites are creatures that invade a host, attach themselves externally and internally (to tissues and organs), and rob the host of nutrients. Symptoms may occur immediately or take up to 20 years for symptoms to manifest. Why in some cases it could be asymptomatic. The presentation of signs and symptoms in some food and take anthelmintic at least one time diseases may involve with helminthic infection. If you are sick with unknown cause, helmenthic infection should be concern. Protection from helminthic infection by eating cooked.

This project was carried out to determine the presence of bla TEM and bla SHV genes in ESBL produ... more This project was carried out to determine the presence of bla TEM and bla SHV genes in ESBL producing Klebsiella pneumonia from urogenital tract of female patients at UBTH. The specific objectives of the study were: (i) to isolate the multi drug resistant strains of Klebsiella pneumonia in urogenital tract of female patient at UBTH,(ii) to identify and characterize ESBLs in the multi-drug resistant Klebsiella pneumonia strains.(iii) to determine what fraction of potential ESBL producers go on to be confirmed.(iv) to determine what fraction of the isolates are hospital or community acquired. Isolates where taken from women who had urogenital infection from both the inward and outward patients, a total of 200 (two hundred) klebsiella pneumoniae isolates which was broken into 100(one hundred) inward patients and 100 (one hundred) outward. Table 2.0 shows the biochemical identification of the isolates, Klebsiella pneumoniae is a lactose fermenter, it is a motile organism, citrate and ur...

The use of these substances otherwise known as aphrodisiacs with capacities to arouse or increase... more The use of these substances otherwise known as aphrodisiacs with capacities to arouse or increase sexual drive or libido and the increasing incidence of male sexual dysfunction is necessitating more and rapid search into plants with aphrodisiacs potentials. Unfortunately, effort in resolving issues about infertility via the consumption of several plant products without recourse to the effects of such substance abuse on the cytoarchitectural and function of vital organs in the body like testis, liver, kidneys and the brain. The aim of this study is to determine the effect of the consumption of aqueous extract of black pepper and ginger as aphrodisiacs on the liver, kidneys and testis of male wistar rats. In all, Thirty-six adult Wistar rats were used for this study. They were divided into four equal groups of nine rats each. Group A served as the control and the rats were given distilled water. Group B was fed with blackpepper extract, Group C was fed with ginger extract and D rats w...

SOJ Immunology, Nov 9, 2017

Recurrent miscarriages of unknown etiology present a challenge to the clinician with a profound s... more Recurrent miscarriages of unknown etiology present a challenge to the clinician with a profound socio-psychological impact in addition to pathophysiological burden for affected couples. Currently, there exists a plethora of articles dealing with genetic polymorphisms associated with recurrent miscarriages with the polymorphic analysis being concentrated on a limited numbers of genes. The NKP 46 gene a major regulator of natural killer cell function has a strong biological literature which suggests a putative role in the immunogenetics of unexplained recurrent miscarriages. Therefore plausible to propose the NKp 46 gene a candidate gene in the etiology of unexplained recurrent miscarriages.

American Journal of Biomedical Sciences, Apr 1, 2017

The use of plant medicine dates back to ancient times, the nearly all year round availability and... more The use of plant medicine dates back to ancient times, the nearly all year round availability and the relatively cheaper cost of medicinal plants in sub Saharan Africa makes them more attractive as therapeutic agents when compared to the orthodox medicine. The use of herbal medicine is on the increase globally, hence the increased interest on research on herbal formulations and preparations. Some of these plants are used as condiments and ingredients in food thus referred to as local spices. One of such plants used as a local spice in Nigeria is Piper guineense traditionally called 'Uziza' amongst the Igbo tribe, 'Iyere' amongst the Yoruba tribe, 'Etinkene' amongst the Efik and Ibibio tribe and 'Ebe-ahinhi akpoke' amongst the Edo tribe. The leaves and seeds of Piper guineense were initially consumed in the Southern part of Nigeria. The aim of this research is to detect and demonstrate any histological effect of Piper guineense leaves on the liver of Wistar rats and on after shave rash in human. During the acclimatization period which lasted for two (2) weeks, three rats died due loss of appetite, emaciation, weakness, dislocation of neck with head leaning to the right, consistent rolling over and vigorous twirling in circular motion during carriage. After two weeks of acclimatization, the rats weighed; The Piper guineense leaves was able to effect total removal of the rash when used on the consented individual within day 3 and day 4 of usage. Piper guineense leaves when taken into reasonably consideration causes no histological changes of the liver tissues, hence its use should be encouraged both for consumption and in treatment of pseudofolliculitis Barbae (after shave rash).

American Journal of Biomedical Sciences, Jul 1, 2017

Cancer is an uncontrolled growth of breast cell, it occurs as a result of mutations or abnormal c... more Cancer is an uncontrolled growth of breast cell, it occurs as a result of mutations or abnormal changes, in the genes responsible for regulating the growth of cells and keeping them healthy. The genes are in each cell. Normally, the cell in our bodies replace themselves through an orderly process of cell growth: healthy new cells take over as old ones die out. A tumor can be benign (not dangerous to health) or malignant (has the potential to be dangerous). Benign tumors are not considered cancerous: their cells are close to normal in appearance, they grow slowly, and they do not invade nearby tissues or spread to other parts of the body. Malignant tumors are cancerous. Left unchecked, malignant cells eventually can spread beyond the original tumor to other parts of the body. Breast cancer can be defined as a malignant tumor of breast usually associated with painless lump in the breast usually associated with painless lump in the breast. Available evidence suggests that breast cancer in most parts of Africa is the leading cause of mortality associated with cancers in African women. Recent global cancer statistics indicates rising global incidence of breast cancer with faster increase occurring in developing countries. To determine the histopathologic cell changes in breast cancer using breast disease tissue blocks in Enugu State University Teaching Hospital 2009-2013. Most women with breast cancer seek medical help at the terminal stage of the disease. This may be a contributory factor to increased number of patient having invasive ductal carcinoma (IDC) as seen in the study. The results of this study suggest that breast cancer is increasing in Nigeria and needs an urgent intervention measure. Cancer cells are treatable if only detected early enough, our findings reveals that breast

ARC Journal of Anesthesiology, 2017

Diabetes mellitus is a metabolic disorder characterized by high glucose levels in the blood. It c... more Diabetes mellitus is a metabolic disorder characterized by high glucose levels in the blood. It constitutes a major health concern today because the prevalence has continuously increased worldwide over the past few decades affecting about 2.8% of the world population. This study examine the palliative effect of daonil supplemented with tryptophan on alloxan induced diabetic rats and the possible benefit of the treatment. Since carbohydrate metabolism is modified in diabetic animals, it's of interest to determine the effect of tryptophan on alloxan induced diabetic rat. To identify possible relationships between tryptophan with diabetes. The purpose of this study was to ascertain if tryptophan administration has positive effect on the glucose level. Twenty five healthy female rats out of 30 were made diabetic by dissolving 150mg alloxan monohydrate per kg weight in 0.9ml of normal saline which was injected into the rats intraperitoneally. After the establishment of diabetes the animals were subdivided into three groups of 4 each. Daonil and tryptophan only were administered orally to group 1 daily for 7 days, Tryptophan only for group 2 and daonil only for group 3 while the group 4 of five served as controls. Tryptophan was administered at 400mg/kg and 0.5ml of daonil. The weight of the animals were recorded, and the glucose levels was determined using the glucometer on the the 3rd and 7th day. Histomorphological studies were conducted on the kidney, liver and pancreas tissues using light microscope method. Daonil and tryptophan treated rats (group1) and Tryptophan only treated rats (group 2), showed no significant decrease (P<0.318) in glucose level. Treatment with daonil showed a significant decrease (P>0.01) in weight and glucose concentration. The histomicrograph .for the three treatments showed that the kidney renal corpuscles appeared as atrophied dense rounded structures bounded by inflammatory infiltrates. Liver show thickened distorted portal vein with clear visible fatty hydropic changes and congested with some visible inflammatory cells. Pancreas pyknotic nuclei of some acinar cells appeared normal with some islet-cells showing congested pyknotic nuclei with lymphocytic infiltrates. The findings in this study indicate that diabetes had deleterious effect on the kidney, liver and pancreas due to drug administration and also tryptophan supplement was not a good alternative for diabetes management.

Sierra Leone Journal of Biomedical Research, 2010

Arcobacters are emerging food borne pathogens potentially associated with prolonged diarrhoea and... more Arcobacters are emerging food borne pathogens potentially associated with prolonged diarrhoea and occasional systemic infections but the pathogenic mechanisms of these bacteria are largely unknown. This study was designed to investigate the pathogenicity of Arcobacter isolates. Two strains of A. butzleri isolated from stool of healthy chickens were confirmed with real time PCR and tested on albino rat by giving a single oral challenge of 10 9 cfu/ml to 65 healthy adult male rats. Five (5) uninfected animals were used as control. Diarrheoal illness occurred in all rats from the fifth day and resolved from day 21 post infection, severe histopathological lesion such as hepatic necrosis, villous erosion, desquamation, matting and necrosis of the segments of small intestine was also observed. In this study, the toxic ileitis necrosis pattern of pathology in the gut of experimentally infected rats could be an indication of observed persistent watery diarrhea associated with the clinical presentation of Arcobacter infection in humans. The pathology of A. butzleri in albino rats had not been previously described, and it appears that the present study is the first report in Nigeria. It may therefore be useful for further investigation.

Emerging Infectious Diseases, 1997

To the Editor: In their excellent article, Molecular Approaches to the Identification of Uncultur... more To the Editor: In their excellent article, Molecular Approaches to the Identification of Unculturable Infectious Agents, Gao and Moore (1) point out that molecular approaches should be unleashed on diseases such as sarcoidosis, Kawasaki disease, and type I diabetes mellitus, which are thought but not proven to be infectious. The authors, however, are overlooking the more common and most likely infectious disease of unknown etiology today-prostatitis. According to the pathologist McNeal, the prostate gland is the most commonly diseased internal organ of the human body (2). Prostatitis is the most common prostate disease, resulting in more physician visits than either benign prostatic hyperplasia or prostate cancer, according to the National Institutes of Health (3). Despite its frequency, prostatitis as a disease and as a histologic lesion is understudied (4). By the Meares and Stamey culture localization procedure, in which the first voided urine, a midstream urine, the expressed prostatic secretions, and a final voided urine are compared, more than 90% of cases in patients with chronic pelvic symptoms are labeled as "nonbacterial" prostatitis or prostatodynia, both of which are thought to be incurable diseases (5). The University of Washington has documented white blood cell counts as high as 38,000 per mm 3 , in "nonbacterial" prostatitis patients (6). According to urologist Thomas Stamey, up to 50% of all men experience symptoms of prostatitis during

Bioresource Technology, 2001

Methyl, ethyl, 2-propyl and butyl esters were prepared from canola and linseed oils through trans... more Methyl, ethyl, 2-propyl and butyl esters were prepared from canola and linseed oils through transesteri®cation using KOH and/ or sodium alkoxides as catalysts. In addition, methyl and ethyl esters were prepared from rapeseed and sun¯ower oils using the same catalysts. Chemical composition of the esters was determined by HPLC for the class of lipids and by GC for fatty acid compositions. The bio-diesel esters were characterized for their physical and fuel properties including density, viscosity, iodine value, acid value, cloud point, pure point, gross heat of combustion and volatility. Methyl and ethyl esters prepared from a particular vegetable oil had similar viscosities, cloud points and pour points, whereas methyl, ethyl, 2-propyl and butyl esters derived from a particular vegetable oil had similar gross heating values. However, their densities, which were 2±7% higher than those of diesel fuels, statistically decreased in the order of methyl $ 2-propyl > ethyl > butyl esters. Butyl esters showed reduced cloud points ()6°C to)10°C) and pour points ()13°C to)16°C) similar to those of summer diesel fuel having cloud and pour points of)8°C and)15°C, respectively. The viscosities of bio-diesels (3:3±7:6 Â 10 À4 Pa s at 40°C) were much less than those of pure oils (22:4±45:1 Â 10 À4 Pa s at 40°C) and were twice those of summer and winter diesel fuels (3.50 and 1:72 Â 10 À4 Pa s at 40°C), and their gross heat contents of approximately 40 MJ/kg were 11% less than those of diesel fuels ($45 MJ/kg). For dierent esters from the same vegetable oil, methyl esters were the most volatile, and the volatility decreased as the alkyl group grew bulkier. However, the bio-diesels were considerably less volatile than the conventional diesel fuels.

Tropical Journal of Natural Product Research, 2019

The ubiquitous nature of plastics has caused increased concerns about the effects of exposure of ... more The ubiquitous nature of plastics has caused increased concerns about the effects of exposure of humans to plastic polymers particularly the endocrine-disrupting chemicals (EDCs) like Di-(2ethylhexyl) phthalate (DEHP) and Bisphenol A (BPA) used in plastic production. The aim of this study was to investigate the effects of BPA and DEHP on haematological and renal function parameters. A total of 60 adult Wistar rats were divided into four groups (I-IV) of 15 animals each. Group I was fed with rodent feed only (control), while groups II-IV were administered 5 mg/kg/day of BPA, 0.5 mg/kg/day of DEHP and a mixture of 0.5 mg/kg/day of BPA and 0.5 mg/kg/day of DEHP, respectively. The animals were monitored for 42 days. The result shows that there were significant (p < 0.05) increase in pack cell volume, haemoglobin, red blood cell, reticulocyte, total white blood cell, lymphocyte, platelet, sodium ion and creatinine concentrations in group II, while the concentrations of urea, potassium and bicarbonate were reduced. In group III, there were increase in the concentrations of pack cell volume, mean cell haemoglobin, and mean cell volume, lymphocyte, platelets, sodium ion, and urea while creatinine concentration was reduced. In group IV, there were increase in the concentration of mean cell haemoglobin concentration, reticulocyte, total white cell count, neutrophil, lymphocyte, platelet, sodium ion and a decrease in creatinine and potassium ion concentration. The present study has shown a possible haematinic effect of BPA and DEHP. They also cause significant alterations in kidney function test parameters.

American Journal of Biomedical Sciences, 2017

A dye is a substance which is used for coloration. Dyes have been in use for ages, nowadays there... more A dye is a substance which is used for coloration. Dyes have been in use for ages, nowadays there are several kinds of dyes used in printing, textiles, food, leather and paints industries. They are also used in Histopathology and cytological examination. This study aims at demonstrating specific component of the cells that will be useful in diagnosis. Solution 1 stains Nuclei-dark blue to black, Cytoplasm stains dark brown to pink fibres-dark brown .The structures were weakly demonstrated by Roselle solution 1. Solution 2 stains Nuclei-dark blue to brown, Cytoplasm-golden brown, RBC-brown, Collagen fibres-golden brown. There was good demonstration of those structures. Roselle Solution 3 stains Nuclei-red, Cytoplasm-golden yellow, RBC-brown, Collagen fibres-pale blue. There was optimal demonstration of those structures. Roselle solution 4 stains Nuclei-red, Cytoplasm-golden yellow, RBC-brown, Collagen fibres-golden yellow, Roselle solution 5 stains Nuclei-blue Cytoplasm-pink. It gives the same color as haematoxylin and eosin technique. Buccal smear and blood smear stains with Roselle plant extract and eosin Nuclei-Blue, Cytoplasm-pink, Barr body-not seen. Conclusively, Roselle plant has the ability to stain tissue sections and staining intensity depends on the type of mordant used.

MOJ Biology and Medicine, 2017

South Asian Research Journal of Medical Sciences

To determine the dangerous blood group "O" donors o reoccurrence at the Federal Teaching Hospital... more To determine the dangerous blood group "O" donors o reoccurrence at the Federal Teaching Hospital, Abakaliki. One hundred (100) cell grouped and sero-typed blood group "0" donors (94 males and 6 females) aged 18 and 45 years participated in this study between February and September 2007 at the Blood Bank(phlebotomy) section of the Teaching Hospital, Abakaliki after an informed consent. The presence of both the alpha () and beta () haemolysin were determined using donors sera for reverse grouping. Haemolysin indicates the presence of haernolysis which is in the sera of the dangerous blood group "O" donors. The study discovered that 36 male donors out of 100 donors studied were positive to haemolysin. This result was statistically analyzed using student"s ttest at 95% confidence interval with P value of 0.05. The numbers of haemolysin positive donors were significantly increased in comparison with the heamolysin negative donors (P< 0.05). This result pattern suggests a relatively high level of blood transfusion reactions in this part of the world. Screening test for dangerous group "O" donors should be carried out routinely in blood transfusion laboratories to order to reduce the occurence of blood transfusion.

International Journal of Biosensors & Bioelectronics

Slowly but surely, healthcare is going digital. Many of the recent innovations in healthcare, fro... more Slowly but surely, healthcare is going digital. Many of the recent innovations in healthcare, from telemedicine and smart devices to the growing capabilities in managing big data, can be traced back to the adoption of new digital technologies that have fostered a different way of working. Across medicine's varied specialty areas, however, the adoption and progress of digital technology advances varies significantly. Digitalization in histopathology laboratory in Nigeria, is still a big setback, but there are digitalization in some of the usual routine practices like the automatic tissue processor which has overtaken the usual manual processor. In the area of sectioning, staining of tissues, mounting of slide and slide reading, we still face major setback in that regard. An improvement in digital diagnostic work, will go a long way in improving basic histopathological practice. From the reported usage of digital tools we can also conclude that possibilities for an overview image, measurements and annotations are much appreciated parts of the digital environment..