Nusrat Jahan Khan 1911878642 - Academia.edu (original) (raw)

Papers by Nusrat Jahan Khan 1911878642

PLOS ONE

The coronavirus disease (COVID-19) pandemic has widely spread worldwide since 2020. Several count... more The coronavirus disease (COVID-19) pandemic has widely spread worldwide since 2020. Several countries have imposed lockdown or stay-at-home policies to prevent the infection. Bangladesh experienced a lockdown from March 2020 to May 2020, and internal travel was restricted. Such long and strict confinement may impact women’s health. Herein, we aimed to assess the impact of the COVID-19 pandemic on women’s health by comparing their health status before and during the pandemic. We conducted a prospective longitudinal study in two zones in the Chhaygaon union, rural district Shariatpur, Bangladesh. The study population comprised non-pregnant women aged 15–49 years. We visited the household of all eligible women and invited them for health checkups. The survey staff examined their health status at the checkup camps and conducted questionnaire interviews. In total, 121 non-pregnant women received health checkups both from June 2019 to July 2019 and in October 2020, before and during the C...

In the rapidly changing times, the need for management development is quite large. To fulfill the... more In the rapidly changing times, the need for management development is quite large. To fulfill these needs the organizations of current time are using various tools and techniques to enhance their organizational behavior and managing their employees. Management development is a structured way used by managers and other employees to enhance their skills and to improve their knowledge. The need for this management development program is that it directly or indirectly benefits the managers and the employees of the organization which in turn is beneficial for the organizations they work for. Management development provides the employees with a stress free environment at their working place and gives employees a satisfied environment. This helps in increasing the productivity of the employee and eventually it leads to increase in productivity of the organization. One of the most important needs of management development is that it helps them to explore their skills and techniques which wo...

This study aimed to explore the practice of SBA class and practical work in classroom situation. ... more This study aimed to explore the practice of SBA class and practical work in classroom situation. Both quantitative and qualitative methods were used for data collection and analysis. A questionnaire survey was carried out on 120 secondary students; 16 teachers' were interviewed and 16 classroom activities were observed using check list and field note method. In addition, necessary documents were analyzed. The result showed that 4 out of 8 schools did not execute class work and practical work at all. Teachers mainly used blackboard for providing instructions to the students for both of these. Class work included written test and questioning and laboratory works or drawing picture was used as practical work. It was reported that participation of students in general and slow learners in particular has improved due to class and practical work. Teachers did not provide feedback immediately but provided it in next class. However, the tasks included in class and practical works could n...

Stem cells translational medicine, Jan 26, 2018

Autologous adipose tissue is used for tissue repletion and/or regeneration as an intact lipoaspir... more Autologous adipose tissue is used for tissue repletion and/or regeneration as an intact lipoaspirate or as enzymatically derived stromal vascular fraction (SVF), which may be first cultured into mesenchymal stem cells (MSCs). Alternatively, transplant of autologous adipose tissue mechanically fragmented into submillimeter clusters has recently showed remarkable efficacy in diverse therapeutic indications. To document the biologic basis of the regenerative potential of microfragmented adipose tissue, we first analyzed the distribution of perivascular presumptive MSCs in adipose tissue processed with the Lipogems technology, observing a significant enrichment in pericytes, at the expense of adventitial cells, as compared to isogenic enzymatically processed lipoaspirates. The importance of MSCs as trophic and immunomodulatory cells, due to the secretion of specific factors, has been described. Therefore, we investigated protein secretion by cultured adipose tissue clusters or enzymatic...

Stem cells and development, 2017

Mesenchymal stem/stromal cells (MSCs) have been used in human and equine regenerative medicine, a... more Mesenchymal stem/stromal cells (MSCs) have been used in human and equine regenerative medicine, and interest in exploiting their potential has increased dramatically over the years. Despite significant effort to characterize equine MSCs, the actual origin of these cells and how much of their native phenotype is maintained in culture have not been determined. In this study, we investigated the relationship between MSCs, derived from adipose tissue (AT) and bone marrow (BM), and pericytes in the horse. Both pericyte (CD146, NG2, and αSMA) and MSC (CD29, CD90, and CD73) markers were detected in equine AT and colocalized around blood vessels. Importantly, as assessed by flow cytometry, both pericyte (CD146, NG2, and αSMA) and MSC (CD29, CD44, CD90, and CD105) markers were present in a majority (≥90%) of cells in cultures of AT-MSCs and BM-MSCs; however, levels of pericyte markers were variable within each of those populations. Moreover, the expression of pericyte markers was maintained ...

Journal of Visualized Experiments, 2016

Stem cells translational medicine, Jan 5, 2016

Perivascular stem cells (PSCs) are the natural ancestors of mesenchymal stem cells (MSCs) and are... more Perivascular stem cells (PSCs) are the natural ancestors of mesenchymal stem cells (MSCs) and are the stem cells responsible for homeostasis and repair in vivo. Prospectively identified and isolated PSCs have demonstrated increased plasticity and osteogenic potential. Cells from the infrapatellar fat pad (IFP) have demonstrated increased chondrogenic potential compared with those from subcutaneous fat. This research assessed the chondrogenic potential of IFP PSCs compared with MSCs from the IFP and bone marrow. Immunohistochemistry demonstrated the location of perivascular markers (CD146, CD34, neural/glial antigen 2 [NG2], platelet-derived growth factor receptor-b [PDGFRb], and a-smooth muscle actin [a-SMA]) in relation to endothelial markers (CD31, CD144, von Willebrand factor [vWF]). Pericytes and adventitial cells were isolated from the stromal vascular fraction (3.8% and 21.2%, respectively) using flow cytometry with a viability of 88%. The mean numbers of pericytes and adventitial cells isolated were 4.6 6 2.2 3 10 4 and 16.2 6 3.2 3 10 4 , respectively, equating to 7.9 6 4.4 3 10 3 and 20.8 6 4.3 3 10 3 cells per gram of harvested tissue. Fluorescence-activated cell sorting demonstrated that cultured PSCs were CD44+CD90+CD105+; polymerase chain reaction and immunocytochemistry demonstrated that pericytes retained their CD146+ phenotype and expressed the pericyte markers PDGFRb and NG2. Differentiation was confirmed using histochemical stains and genetic expression. Using a pellet model, the IFP PSCs and the MSCs generated significantly more extracellular matrix than bone marrow MSCs (p < .001 and p = .011, respectively). The IFP PSCs generated significantly more extracellular matrix than IFP MSCs (p = .002). Micromass culture demonstrated that differentiated PSCs were upregulated compared with MSCs for COL2A1, ACAN, and SOX9 expression by factors of 4.8 6 1.3, 4.3 6 0.9, and 7.0 6 1.7, respectively. The IFP was a significantly better source of chondrogenic stem cells compared with bone marrow. PSCs generated significantly more extracellular matrix than culturederived MSCs.

Stem cells and development, Jan 23, 2016

Previous research has indicated that purified perivascular stem cells (PSCs) have increased chond... more Previous research has indicated that purified perivascular stem cells (PSCs) have increased chondrogenic potential compared to conventional mesenchymal stem cells (MSCs) derived in culture. This study aimed to develop an autologous large animal model for PSC transplantation and to specifically determine if implanted cells are retained in articular cartilage defects. Immunohistochemistry and fluorescence-activated cell sorting were used to ascertain the reactivity of anti-human and anti-ovine antibodies, which were combined and used to identify and isolate pericytes (CD34(-)CD45(-)CD146(+)) and adventitial cells (CD34(+)CD45(-)CD146(-)). The purified cells demonstrated osteogenic, adipogenic, and chondrogenic potential in culture. Autologous ovine PSCs (oPSCs) were isolated, cultured, and efficiently transfected using a green fluorescence protein (GFP) encoding lentivirus. The cells were implanted into articular cartilage defects on the medial femoral condyle using hydrogel and colla...

Journal of the College of Physicians and Surgeons--Pakistan : JCPSP, 2011

To evaluate the effect of mother-infant early skin-to-skin contact on breastfeeding behavior of i... more To evaluate the effect of mother-infant early skin-to-skin contact on breastfeeding behavior of infants. A randomized controlled trial. The study was conducted in the Department of Obstetrics of Pakistan Institute of Medical Sciences, Islamabad, from November to December 2009. Eligible mothers were assessed for the successful breastfeeding by using IBFAT tool. The time to initiate the first feed, time to effective breastfeeding, maternal satisfaction with the care provided, preference for the same care in future and level of exclusive breastfeeding at the age of one month were also noted. The data was compared by using X2 and t-test. Significant p-value was taken as < 0.05. A total of 183 mother-infant pairs (92 in skin-to-skin care [SSC] group and 91 in conventional care [CC] group) were analyzed for breastfeeding behavior of the infants. The first breastfeed was 26.25% more successful in SSC group (58.8% in SSC group as compared to 32.5% in CC group with p-value of 0.001). In S...

The New phytologist, 2015

In wheat stems, the levels of fructan-dominated water-soluble carbohydrates (WSC) do not always c... more In wheat stems, the levels of fructan-dominated water-soluble carbohydrates (WSC) do not always correlate well with grain yield. Field drought experiments were carried out to further explain this lack of correlation. Wheat (Triticum aestivum) varieties, Westonia, Kauz and c. 20 genetically diverse double haploid (DH) lines derived from them were investigated. Substantial genotypic differences in fructan remobilization were found and the 1-FEH w3 gene was shown to be the major contributor in the stem fructan remobilization process based on enzyme activity and gene expression results. A single nucleotide polymorphism (SNP) was detected in an auxin response element in the 1-FEH w3 promoter region, therefore we speculated that the mutated Westonia allele might affect gene expression and enzyme activity levels. A cleaved amplified polymorphic (CAP) marker was generated from the SNP. The harvested results showed that the mutated Westonia 1-FEH w3 allele was associated with a higher thousa...

International Journal of Rehabilitation and Health, 1998

The purpose of this study was to determine factors associated with nonuse of an AK prosthesis in ... more The purpose of this study was to determine factors associated with nonuse of an AK prosthesis in a rural, Southern, geriatric population. We conducted a telephone survey of AK amputees fitted with a prosthesis in Eastern North Carolina. There was an association between nonuse of the prosthesis and the following factors: African-American race; low education; no gainful employment; copresence of

International Journal of Infectious Diseases, 2007

Introduction: Human papillomavirus (HPV) is recognized as a major causative agent for cervical ca... more Introduction: Human papillomavirus (HPV) is recognized as a major causative agent for cervical carcinomas. Based on their oncogenic potential, HPV subtypes have been divided into high-and low-risk. In Pakistan, screening for HPV in female patients is not commonly practiced, and as a consequence, the degree of HPV prevalence and its correlation with cervical cancer is unknown. Objective: In this study, we have attempted to estimate the prevalence of HPV infection, and also the HPV subtype profile, among Pakistani women with cervical cancer from varied geographical, racial, and social backgrounds within Pakistan. Methodology: Women visiting two tertiary care hospitals in Karachi, diagnosed with carcinoma of the cervix within the past 15 years, were analyzed for HPV subtypes in their cancer specimens. Retrospectively, 60 paraffin-embedded cervical cancer biopsies were examined for the presence of HPV DNA. After DNA extraction from these samples, polymerase chain reaction (PCR) was used to amplify the HPV L1 gene using the consensus (general) primers, and primers specific for subtypes 16 and 18. Results: Of the 60 samples analyzed, only one sample was HPV negative; the rest of the samples were positive for the presence of HPV. Of the 59 HPV positive samples, 56 showed the presence of HPV16 and one sample was positive for HPV18; HPV subtype could not be determined in two samples. Conclusion: Our results show a strong relationship between HPV infection and cervical cancer among Pakistani women. These results underscore the need to implement regular HPV screening for Pakistani women. An early diagnosis of HPV infection will allow better health management to reduce the risk of developing cervical cancer.

Euphytica, 2014

ABSTRACT The major vernalisation genes of VRN1 are well understood at the molecular level. Howeve... more ABSTRACT The major vernalisation genes of VRN1 are well understood at the molecular level. However, their quantitative contributions to flowering time and grain yield related traits are not clear. In this study, we used a double haploid population (225 lines) of Westonia x Kauz in which the Vrn-A1a (Westonia), Vrn-B1a (Westonia) and Vrn-D1a (Kauz) were segregating, and a high resolution genetic map of 1159 loci, to determine the quantitative contributions of Vrn-A1a, Vrn-B1a and Vrn-D1a for the days to anthesis and grain yield related traits in diverse environments. The major quantitative trait loci (QTL) of spikelet number per spike and days to anthesis were contributed by the winter alleles of VRN1. The QTL of the time of grain filling were contributed by the spring alleles of VRN1. The wild genotype (vrn-A1vrn-B1vrn-D1) showed the latest flowering, the highest spikelet number per spike, lowest peduncle proportion and thousand grain weight in three environmental analyses, and the largest spikelet number per spike, which resulted in high kernel number per spike (KN) and grain weight (GW) in well-watered environments. One QTL of KN was located on 5B, contributed by winter allele of vrn-B1 in three environmental analyses, and one GW QTL was detected on 5A, contributed by the spring allele of Vrn-A1a in a drought environment. The results indicated that the genotype Vrn-A1avrn-B1Vrn-D1a would shorten the time to anthesis and give high GW and KN in drought environments. The early anthesis associated phenotype, peduncle proportion would provide an indicator in breeding programs.

Cytometry Part A, 2013

Mesenchymal stem/stromal cells (MSCs) are adult multipotent progenitors of great promise for cell... more Mesenchymal stem/stromal cells (MSCs) are adult multipotent progenitors of great promise for cell therapy. MSCs can mediate tissue regeneration, immunomodulation, and hematopoiesis support. Despite the unique properties of MSCs and their broad range of potential clinical applications, the very nature of these cells has been uncertain. Furthermore, MSCs are heterogeneous and only defined subpopulations of these are endowed with the particular abilities to sustain hematopoietic stem cells, regulate immune responses, or differentiate into mesodermal cell lineages. It is becoming evident that current criteria used to define cultured polyclonal MSCs (expression of nonspecific markers and in vitro mesodermal differentiation) are not sufficient to fully understand and exploit the potential of these cells. Here, we describe how flow cytometry has been used to reveal a perivascular origin of MSCs. As a result, the prospective purification of MSCs and specialized subsets thereof is now possible, and the clinical use of purified autologous MSCs is now within reach. V

Journal of the College of Physicians …, 2010

OBJECTIVE: To measure the frequency of ophthalmia neonatorum among all babies born in MCH Center,... more OBJECTIVE: To measure the frequency of ophthalmia neonatorum among all babies born in MCH Center, PIMS (Pakistan Institute of Medical Sciences), Islamabad, in one month's time and to determine their causative organisms. STUDY DESIGN: An observational study. ...

Clinical Pediatrics, 2001

S ubcutaneous fat necrosis (SCFN) of the newborn is an uncommon yet widely recognized disorder, o... more S ubcutaneous fat necrosis (SCFN) of the newborn is an uncommon yet widely recognized disorder, of unclear etiology in which skin lesions de-velop often over bony promi-nences and extremities. It often occurs in full-term neonates with a history of difficulty during birth ...

British Journal of Psychiatry, 2007

BackgroundWith almost a million people dying by suicide worldwide each year, reducing the rate of... more BackgroundWith almost a million people dying by suicide worldwide each year, reducing the rate of suicidal behaviour is a priority in many countries.AimsTo examine whether additional psychosocial interventions following an episode of self-harm reduce the likelihood of subsequent suicide.MethodWe conducted a systematic review and meta-analysis of data from randomised controlled trials of interventions for people following self-harm. Likelihood of suicide was compared by calculating the pooled root difference in suicide rate with 95% confidence intervals.ResultsWe obtained suicide data from 18 studies with a total population of 3918. Eighteen suicides occurred among people offered active treatment and 19 among those offered standard care (pooled root difference in suicide rate 0.0, 95% CI – 0.03 to 0.03). The overall rate of suicide among people participating in trials was similar to that reported in observational studies of people who self-harm.ConclusionsResults of this meta-analysi...

Archives of Internal Medicine, 2001