OKPALA JUDE CHINEDU - Academia.edu (original) (raw)
I am a medical Biochemist with a research focus on medicinal plants, antioxidants study, cancer and diabetes. I have a passion for saving lives and serving humanity through quality medical research tailored towards finding solutions to medical challenges.
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Effects of n-butanol fraction of Gongronema latifolium leave extract on some liver function and h... more Effects of n-butanol fraction of Gongronema latifolium leave extract on some liver function and histological parameters in CCl 4-induced oxidative damage in Wistar albino rats were assessed. Fifty-four (54) Wistar albino rats were divided into treatment group and LD 50 groups. Group A (normal control) was given feed and water, Group B (vehicle control) was injected with olive oil intraperitoneally, while the rest groups (C, D, E, F and G) were injected intraperitoneally with a single dose of CCl 4 (148 mg/kg) as a 1:1 (v/v) solution in olive oil and all the animals were fasted for 36 hours. This was repeated once every week for a period of four (4) weeks. At the end of 28 days of treatment, liver marker enzymes studies showed that there was significant (p<0.05) increase in the serum activities of ALT, AST, ALP and bilirubin concentrations in CCl 4-induced control group when compared with the normal control and induced treated groups but there was no significant (p>0.05) difference of these liver marker enzymes and bilirubin levels between the normal control and induced treated groups. Antioxidant assay on the liver homogenate showed that there was significant (p<0.05) decrease in SOD, CAT, GPx and a significant increase (p<0.05) in MDA of CCl 4-induced control rats when compared to the normal control rats but there was no significant (p>0.05) difference between the normal control and induced treated groups. These findings suggested that n-butanol fraction of methanolic leave extract of G. latifolium may have anti-hepatotoxic and antioxidative effects against CCl 4-induced liver damage in rats.
Effects of n-butanol fraction of Gongronema latifolium leave extract on some liver function and h... more Effects of n-butanol fraction of Gongronema latifolium leave extract on some liver function and histological parameters in CCl 4-induced oxidative damage in Wistar albino rats were assessed. Fifty-four (54) Wistar albino rats were divided into treatment group and LD 50 groups. Group A (normal control) was given feed and water, Group B (vehicle control) was injected with olive oil intraperitoneally, while the rest groups (C, D, E, F and G) were injected intraperitoneally with a single dose of CCl 4 (148 mg/kg) as a 1:1 (v/v) solution in olive oil and all the animals were fasted for 36 hours. This was repeated once every week for a period of four (4) weeks. At the end of 28 days of treatment, liver marker enzymes studies showed that there was significant (p<0.05) increase in the serum activities of ALT, AST, ALP and bilirubin concentrations in CCl 4-induced control group when compared with the normal control and induced treated groups but there was no significant (p>0.05) difference of these liver marker enzymes and bilirubin levels between the normal control and induced treated groups. Antioxidant assay on the liver homogenate showed that there was significant (p<0.05) decrease in SOD, CAT, GPx and a significant increase (p<0.05) in MDA of CCl 4-induced control rats when compared to the normal control rats but there was no significant (p>0.05) difference between the normal control and induced treated groups. These findings suggested that n-butanol fraction of methanolic leave extract of G. latifolium may have anti-hepatotoxic and antioxidative effects against CCl 4-induced liver damage in rats.