Olena Vergun - Academia.edu (original) (raw)
Papers by Olena Vergun
Poľnohospodárstvo, Jun 30, 2023
Agrobiodiversity for improving nutrition, health and life quality, May 1, 2024
Genetičnì resursi roslin, 2023
Agrobiodiversity for improving nutrition, health and life quality, Dec 1, 2022
Plants of Vigna Savi genus are widely used in the world as food and medicinal plants. Most invest... more Plants of Vigna Savi genus are widely used in the world as food and medicinal plants. Most investigations propagated the biochemical composition and biological activity of seeds of these plants due to the high content of proteins but fewer studies about the above-ground plant part. The aim of this study focused on the investigation of the antioxidant activity of Vigna spp. The plant raw of Vigna angularis (Willd.) Ohwi & H. Ohashi, V. mungo (L.) Hepper, V. radiata (L.) Wilczek, V. unguiculata (L.) Walp. was collected from the M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine experimental collection at the start of the vegetation and flowering stage. At the start of vegetation was determined 45.27-77.21 mg GAE.g-1 (gallic acid equivalent) of total polyphenol compounds (TPC), 8.67-20.48 mg CAE.g-1 (caffeic acid equivalent) of total phenolic acids (TPAC), 31.84-47.97 mg QE.g-1 (quercetin equivalent) of total flavonoids content (TFC), 6.97-8.14 mg TE.g-1 (Trolox equivalent) of antioxidant activity by DPPH method, and 110.52 to 142.61 mg TE.g-1 of AA by phosphomolybdenum method. At the flowering stage found the following results: 27.16-78.11 mg GAE.g-1 of polyphenol compounds, 4.97-17.16 mg CAE.g-1 of phenolic acids, 18.27-54.26 mg QE.g-1 of flavonoids, 4.6-6.69 mg TE.g-1 of AA by DPPH method, and 45.16-110.27 mg TE.g-1 of antioxidant activity by phosphomolybdenum method. A very strong correlation found between antioxidant activity by phosphomolybdenum method and TPAC (r = 0.883), TPC (r = 0.858), and TFC (r = 0.843) at the flowering stage. These results can be used for further biochemical and pharmacological investigations of these plants.
Agrobiodiversity for improving nutrition, health and life quality, Dec 1, 2022
The review of previous studies on Paulownia spp. showed that these plants are valuable raw materi... more The review of previous studies on Paulownia spp. showed that these plants are valuable raw materials with polyfunctional use among which are medicinal, forage, energetic, etc. This study demonstrated the accumulation of selected biochemical components in the different parts of Paulownia tomentosa (Thunb.) Steud. genotypes plants by the end of vegetation collected from experimental collections of M. M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine. The accumulation of selected nutrients in the leaves was the following:
Zenodo (CERN European Organization for Nuclear Research), Mar 1, 2019
Objective-to investigate the accumulation of biochemical compounds in the different organs of cul... more Objective-to investigate the accumulation of biochemical compounds in the different organs of cultivars and varieties of Miscanthus Anderss. in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. Investigated plants were cultivars and varieties of species of the genus of Miscanthus collected in an experimental collection of Cultural Flora Department of M.M. Gryshko National Botanical Garden of the NAS of Ukraine: М.
Journal of Native and Alien Plant Studies, 2021
Intr0ducti0n. Because plants are an important factor in the existence of the biosphere and life o... more Intr0ducti0n. Because plants are an important factor in the existence of the biosphere and life on Earth, plant resources are an invaluable asset that requires comprehensive study, conservation and rational use in the context of global climate change. Matcrials and mcth0ds. The subject of research was the collection of spice, essential, medicinal, energy, oilseeds, sugar plants. The object of the study was the process of introduction, selection, conservation and enrichment of genetic resources of energy and aromatic plants. Methods of introduction, acclimatization, adaptation, selection, biotech- nology are used. Physiological, biochemical, allelopathic, and ecological methods were also used. Rcsults and discus- si0n. For many years of introduction and selection work in the department of cultural flora of NBG collected valuable gene pool of useful plants (about 2000 taxa) part of which, namely “Collection Fund of Energy and Aromatic Plants of the National Botanical Garden of the Nat...
Agrobiodiversity for improving nutrition, health and life quality, Nov 1, 2018
The economically important species of genus Brassica L. were investigated in this study. Alcohol ... more The economically important species of genus Brassica L. were investigated in this study. Alcohol and water plant extracts of cultivars and varieties of Brassica campestris and B. rapa were inspected on antiradical activity by DPPH-method (with 2,2-diphenyl-2-picrylhydrazyl). Obtained extracts were measured on a spectrophotometer at wavelength 515 nm. Antiradical activity of methanol extracts was in the range from 34.29 (B. rapa subsp. rapifera Metzger (f. biennis), f. EOTRFO) to 58.09% (B. campestris f. biennis D.C., cv. Oriana). Water extracts demonstrated this activity in range from 58.18 (B. campestris f. biennis D.C., cv. Oriana) to 84.25% (B. campestris f. biennis D.C. × B. rapa L., f. EOTFVS). Antioxidant activity (AA) of extracts of investigated plants was expressed in ascorbic acid equivalent (mg g-1 AAE). The highest AA of methanol extracts was found for B. campestris f. biennis D.C. × B. rapa L., cv. Fitopal (86.07 mg g-1 AAE), the lowest one-for B. rapa subsp. rapifera Metzger (f. biennis), f. EOTRFO (52.78 mg g-1 AAE). Maximal AA of water extracts was registered for B. campestris f. biennis D.C. × B. napus f. biennis D.C., cv. Innovacia (121.77 mg g-1 AAE), minimal-for B. campestris f. biennis D.C. × B. rapa L., f. EOTFVS (85.18 mg g-1 AAE).
Zenodo (CERN European Organization for Nuclear Research), Dec 1, 2017
Conclusions. Obtained data showed that in conditions of M.M. Gryshko National Botanical Garden of... more Conclusions. Obtained data showed that in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine the genus Miscanthus plants is a valuable source of nutrients the most content of which observed in the tillering period. Due to this fact these plants can be recommended as nutritional supplements for animal feed. In addition to the aforementioned content of dry matter, total content of sugars of investigated plants of the genus Miscanthus increased from tillering to seed ripening stage. It is found that content of carotene, ascorbic acid, calcium decreased from tillering to seed ripening stage. The total content of ash decreased to seed ripening phase but not significantly. The level of phosphorus varied during vegetation in investigated plants.
Plant Introduction, Mar 1, 2018
The antioxidant activity of methanol and aqueous extracts based on the discoloration reaction on ... more The antioxidant activity of methanol and aqueous extracts based on the discoloration reaction on the solution of DPPH (2,2-diphenyl-1-picrylhydrazyl free radical) was determined according to Brandt-Williams et al.
Plant varieties studying and protection, May 30, 2022
To conduct a comprehensive study of morphological features of the genus Vigna plants, to assess t... more To conduct a comprehensive study of morphological features of the genus Vigna plants, to assess their productive potential depending on the species characteristics under the conditions of introduction in the Right-Bank Forest-Steppe of Ukraine. Methods. The main method of work was a comparative morphological analysis of plants grown from seeds. The research was carried out at the introduction sites and in the laboratory of the Department of Cultural Flora of the
![Research paper thumbnail of Evaluation of the antioxidant activity and phenolic content of Chinese quince (Pseudocydonia sinensisSchneid.) fruit [pdf]](https://attachments.academia-assets.com/107826096/thumbnails/1.jpg)
](Acta Scientiarum Polonorum, Mar 30, 2020
Background. Neglected and underutilized plant species could serve as a valuable source of natural... more Background. Neglected and underutilized plant species could serve as a valuable source of natural bioactive compounds. The objective of this study was to evaluate the biological activity of Chinese quince (Pseudocydonia sinensis Schneid) genotypes of Ukrainian and Slovak origin. Materials and methods. The content of the total antioxidant activity (DPPH method and molybdenum reducing antioxidant power), total polyphenol, flavonoid and phenolic acid compounds in the pulp and peel of Chinese quince were compared across five genotypes from Slovakia and three from Ukraine. Results. All tested samples exhibited DPPH • radical scavenging activities with values from 6.17 to 9.56 mg TEAC (Trolox equivalent antioxidant capacity) per gram of dry matter (DM). Antioxidant activity, measured using the molybdenum reducing antioxidant power method, ranged from 69.82 to 225.04 mg TEAC per gram of DM. Total polyphenol content was from 34.73 to 82.02 mg GAE (gallic acid equivalent), while total flavonoid content was from 0.50 to 26.72 mg QE (quercetin equivalent) per gram DM. Phenolic acid content varied from 1.12 to 8.39 mg CAE (caffeic acid equivalent) per gram DM. The peel extracts contained the highest content of bioactive compounds when compared with the pulp extract (from 15.30 to 32.60%). All observed parameters differed significantly between the genotypes. Strong positive correlations (p ≤ 0.05) were observed between the content of phenolic acids and flavonoids in the peel in plants from Slovakia (r = 0.951, r = 0.928, respectively); between the phenolic acid and antioxidant capacities detected using the MRP method-r = 0.950 and r = 0.955 for peel and pulp, respectively; between the determination of antioxidant activity by the DPPH and MRP methods in the peel and pulp in plants from Ukraine (r = 0.986, r = 0.998, respectively). Significantly positive correlations were found between all the parameters in the samples of Ukrainian origin. Conclusion. The results showed that all fruit extracts exhibited strong antioxidant activities, which generally correlated positively with the total phenolic content. This study demonstrates that Chinese quince fruit grown in Ukraine and Slovakia is a perspective source of valuable polyphenol content with high antioxidant activity and is a valuable fruit for use in the agriculture and food industries.
Plant varieties studying and protection, Mar 30, 2023
Ñåëåêö³ÿ òà íàñ³ííèöòâî Селекція елекція та насінництво та насінництво С Селекція елекція та насі... more Ñåëåêö³ÿ òà íàñ³ííèöòâî Селекція елекція та насінництво та насінництво С Селекція елекція та насінництво та насінництво Âñòóï Ïîñòóïîâ³ êë³ìàòè÷í³ çì³íè é àðèäèçàö³ÿ çíà÷íèõ òåðèòîð³é ó âñüîìó ñâ³ò³-íàéãîëîâ-í³ø³ ïðîáëåìè ñüîãîäåííÿ, ùî ïîòðåáóþòü ñâîº÷àñíîãî òà âñåá³÷íîãî âèð³øåííÿ [1-4].
Plant varieties studying and protection, Dec 29, 2021
Ñîðòîâèâ÷åííÿ òà ñîðòîçíàâñòâî Âñòóï Ðîäèíà Fabaceae-ðîñëèíè ³ç âèñîêèì âì³ñòîì ïîaeèâíèõ ðå÷îâèí... more Ñîðòîâèâ÷åííÿ òà ñîðòîçíàâñòâî Âñòóï Ðîäèíà Fabaceae-ðîñëèíè ³ç âèñîêèì âì³ñòîì ïîaeèâíèõ ðå÷îâèí, çîêðåìà ïðîòå¿íó, à òàêîae á³îàêòèâíèìè ô³òîåñòðîãåíàìè, ùî ðîáèòü ¿õ ïåðñïåêòèâíèìè äëÿ âèêîðèñòàííÿ ó ð³çíèõ ñôåðàõ ãîñïîäàðþâàííÿ [1]. Âèäè ðîäó Galega º îäíèìè ³ç íàéö³íí³øèõ é âîäíî÷àñ ìàëîâèâ÷åíèìè ïðåäñòàâíèêàìè ö³º¿ ðîäèíè [2]. Ðîñëèíè âèäó G. orientalis äîñë³äaeóþòüñÿ, çîêðåìà, ÿê êîðìîâ³ [3-7] çàâäÿêè ¿õí³é âè-ñîê³é ïðîäóêòèâíîñò³ [8], ñò³éêîñò³ â ð³çíèõ êë³ìàòè÷íèõ óìîâàõ, åêîíîì³÷í³é ðåíòà
Plant varieties studying and protection, Apr 6, 2021
Purpose. Determine a number of morphometric and biochemical parameters of various genotypes of Bu... more Purpose. Determine a number of morphometric and biochemical parameters of various genotypes of Bunias orientalis L. in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG). Methods. Plant samples of B. orientalis (6 genotypes created in the NBG) were examined during the flowering stage. Determination of dry matter, ash, calcium was carried out according to Hrytsaienko et al. (2003), phosphorus according to Pochinok (1976), sugars, ascorbic acid and lipids were determined according to Krishchenko (1983), β-carotene according to Pleshkov (1985). The energy value of plants was determined using an IKA C-200 calorimeter. The obtained results were analysed statistically. Results. The height of plants varied from 140.9 (Genotype 1) to 157.5 (Genotype 5) cm, stem diameter from 11.67 (Genotype 1) to 16.1 (Genotype 6) mm, the number of internodes from 18.7 (Genotype 1) to 25.7 (Genotype 6), the number of leaves on a stem from 14.11 (Genotype 1) to 21.8 (Genotype 5), leaf lamina length from 14.2 (Genotype 1) to 23.45 (Genotype 6) cm, leaf lamina width from 6.34 (Genotype 1) to 14.5 (Genotype 4) cm, inflorescence length from 27.4 (Genotype 1) to 45.4 (Genotype 3) cm, inflorescence width from 2.32 (Genotype 1) to 4.92 (Genotype 3) cm, and the number of stems from 2.55 (Genotype 2) to 5.33 (Genotype 1). The study of the content of structural and functional compounds and nutrients at the flowering stage showed that the dry matter content was in the range of 13.58-16.00%, sugars 5.07-8.86%, titratable acidity 3.28-4.25%, lipids 3.33-6.61%, ascorbic acid 382.83-693.82 mg%, β-carotene 0.94-3.48 mg%, ash 6.79-9.2%, calcium 1.00-2.44%, phosphorus 1.61-2.67% and energy value 3337.0-3498.0 cal/g. Conclusions. It was revealed that samples of various genotypes of B. orientalis are a valuable source of nutrients at the flowering stage. The biochemical composition of plants depended on the genotype and stage of growth. Results of the morphometric study showed variability of investigated parameters. The obtained data can be used to predict and evaluate the results of introduction and breeding studies with B. orientalis genotypes as promising crops in Ukraine.
Plant varieties studying and protection, Jul 15, 2019
Ô³ç³îëîã³ÿ ðîñëèí Âñòóï Çáåðåaeåííÿ, çáàãà÷åííÿ òà åôåêòèâíå âèêîðèñòàííÿ ðîñëèííèõ ðåñóðñ³â ìຠ... more Ô³ç³îëîã³ÿ ðîñëèí Âñòóï Çáåðåaeåííÿ, çáàãà÷åííÿ òà åôåêòèâíå âèêîðèñòàííÿ ðîñëèííèõ ðåñóðñ³â ìຠâàaeëèâå íàóêîâå òà ïðàêòè÷íå çíà÷åííÿ. Çâàaeàþ÷è íà öå, ³íòðîäóêö³ÿ íîâèõ íåòðàäèö³éíèõ ìàëîïîøèðåíèõ âèä³â ðîñëèí ³ ðîçðîáëÿííÿ á³î-ëîã³÷íèõ, á³îõ³ì³÷íèõ, á³îòåõíîëîã³÷íèõ îñíîâ ââåäåííÿ ¿õ ó ïðîìèñëîâó òà àìàòîðñüêó êóëüòóðó º íàäçâè÷àéíî âàaeëèâèì çàâäàííÿì. Äî ïåðñïåêòèâíèõ äëÿ ³íòðîäóêö³¿ âèä³â ðîñëèí íàëåaeàòü ïðåäñòàâíèêè ðîäó Serratula L., ùî íàðàõîâóº ïðèáëèçíî 70 âèä³â, ïîøèðåíèõ ó ªâðà糿 ³ ϳâí³÷í³é Àôðèö³ [1].  Óê-ðà¿í³ òðàïëÿþòüñÿ ï'ÿòü âèä³â ðîäó Serratula [2]. Âèäè ðîäó Serratula L., íàñàìïåðåä Serratula coronata L., â³äçíà÷àþòüñÿ âèñîêî-ö³ííîþ ô³òîñèðîâèíîþ, à òîìó º ïåðñïåêòèâíèìè äëÿ âèêîðèñòàííÿ â ìåäèöèí³ ÷åðåç âè-ÿâëåí³ â íèõ á³îëîã³÷íî àêòèâí³ ðå÷îâèíè-ô³òîåêäèñòåðî¿äè. Âèâ÷åííÿ á³îõ³ì³÷íèõ îñî
Zenodo (CERN European Organization for Nuclear Research), Aug 1, 2017
Objective-to establish biological and biochemical properties of plants of the genus Agrostis L. s... more Objective-to establish biological and biochemical properties of plants of the genus Agrostis L. species in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. The objects of these investigations were species and cultivars of the genus Agrostis species. It was conducted morphometric measurements and some parameters of the productivity in the flowering stage.
Ìntrodukcìâ roslin, Apr 28, 2022
Cosmos sulphureus is an adventitious species for Europe and Ukraine in particular. It originates ... more Cosmos sulphureus is an adventitious species for Europe and Ukraine in particular. It originates from Central and South America, where it grows in tropical and subtropical climates. The climatic conditions of Kyiv are characterized as temperate, with an absolute temperature minimum of-32.2 °C, an absolute maximum of +39.9 °C, and average annual rainfall of 649 mm. Plants were analyzed in the flowering phase, divided into inflorescences, leaves, stems, and roots, dried at +35 °C and then extracted with methanol and water. The determination of the antiradical activity was carried out according to a modified method using a DPPH (2,2-diphenyl-1-picrylhydrazyl) radical inhibition reaction. The highest antiradical activity was detected in inflorescences (59.60-81.81 % inhibition) and leaves (79.81-89.12 % inhibition). Stem extracts had an average level of inhibition (19.63-65.93 %), and root extracts showed only 2.54-39.46 % inhibition. Correlation analysis showed a strong relationship between leaves and stems (r = 0.84), leaves and roots (r = 0.81), and stems and roots (r = 0.91). Extracts of C. sulphureus plants grown in temperate climate were found having a high antioxidant potential but lower than that reported for tropical and subtropical regions. It was found that higher intensity of coloration of marginal florets of the capitulum does not correlate with a higher antiradical activity. Methanolic and water extracts of inflorescences of the genotype CSCO-368812 with intensilly colored perianth inhibited only 59.60 % and 71.50 % of radicals, while similar extracts of the genotype CS-361294 with lighter florets inhibited 71.17 % and 81.81 % of radicals, respectively. Instead, there was a difference in antiradical activity depending on applied extractant. Methanolic extracts of vegetative organs (leaves, stems, roots) prevail over water extracts in terms of their antiradical activity. However, water extracts of inflorescences of both genotypes demonstrated higher level of antiradical activity.
![Research paper thumbnail of Antioxidant activities and phenolic compounds in fruits of various genotypes of American persimmon (Diospyros virginiana L.) [pdf]](https://attachments.academia-assets.com/107826091/thumbnails/1.jpg)
](Acta Scientiarum Polonorum, Jun 30, 2018
Background. American persimmons (Diospyros virginiana L.) are known as a widespread cultivar whic... more Background. American persimmons (Diospyros virginiana L.) are known as a widespread cultivar which were traditionally used by Native Americans as a food source, and since ancient times have been used in folk medicine. The objective of this study was to evaluate the antioxidant activity and phenolic content of Diospyros virginiana genotypes. Material and methods. The content of the total antioxidant activity and phenolic compounds from the fruits of the American persimmon (Diospyros virginiana L.) of six genotypes were compared. Antioxidant activity (AOA) was measured using three different photometric methods-DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) and FRAP (ferric-reducing antioxidant power). Total phenolic content (TPC) was evaluated using Folin-Ciocalteu reagent assay. Results. The results for AOA (μMol Trolox/g) determined by the DPPH method varied from 51.68 (DV-05) to 100.87 (DV-03), those obtained by the ABTS method varied from 65.40 (DV-05) to 142.26 (DV-03), and those obtained by the FRAP method varied from 45.06 (DV-05) to 109.30 (DV-03). The results for TPC varied from 590.75 ±27.98 mg/100 g (DV-05) to 1325.12 ±77.30 mg/100 g (DV-03). The highest results for AOA and TPC were achieved for the fruits of genotypes DV-03 and DV-05. A positive linear correlation was found between antioxidant activity and total phenolic content in the examined plant material. Conclusion. The results showed that all fruit extracts exhibited strong antioxidant activities, which generally correlated positively with the total phenolic content. This study demonstrates the potential of the fruits of Diospyros virginiana grown in Ukraine as a possible source of valuable polyphenol content, with high antioxidant activities and health-promoting properties. The high contents of phenolic compounds and significant linear correlation between the values of the concentration of phenolic compounds and antioxidant activity indicated that these compounds contributed to the strong antioxidant activity.
Plant varieties studying and protection, Jan 31, 2023
Plant physiology Фізіологія рослин ізіологія рослин Ф Фізіологія рослин ізіологія рослин Introduc... more Plant physiology Фізіологія рослин ізіологія рослин Ф Фізіологія рослин ізіологія рослин Introduction The biochemical analysis of both introduced and well-known medicinal and aromatic plants is an important task [1, 2]. This is because of
Poľnohospodárstvo, Jun 30, 2023
Agrobiodiversity for improving nutrition, health and life quality, May 1, 2024
Genetičnì resursi roslin, 2023
Agrobiodiversity for improving nutrition, health and life quality, Dec 1, 2022
Plants of Vigna Savi genus are widely used in the world as food and medicinal plants. Most invest... more Plants of Vigna Savi genus are widely used in the world as food and medicinal plants. Most investigations propagated the biochemical composition and biological activity of seeds of these plants due to the high content of proteins but fewer studies about the above-ground plant part. The aim of this study focused on the investigation of the antioxidant activity of Vigna spp. The plant raw of Vigna angularis (Willd.) Ohwi & H. Ohashi, V. mungo (L.) Hepper, V. radiata (L.) Wilczek, V. unguiculata (L.) Walp. was collected from the M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine experimental collection at the start of the vegetation and flowering stage. At the start of vegetation was determined 45.27-77.21 mg GAE.g-1 (gallic acid equivalent) of total polyphenol compounds (TPC), 8.67-20.48 mg CAE.g-1 (caffeic acid equivalent) of total phenolic acids (TPAC), 31.84-47.97 mg QE.g-1 (quercetin equivalent) of total flavonoids content (TFC), 6.97-8.14 mg TE.g-1 (Trolox equivalent) of antioxidant activity by DPPH method, and 110.52 to 142.61 mg TE.g-1 of AA by phosphomolybdenum method. At the flowering stage found the following results: 27.16-78.11 mg GAE.g-1 of polyphenol compounds, 4.97-17.16 mg CAE.g-1 of phenolic acids, 18.27-54.26 mg QE.g-1 of flavonoids, 4.6-6.69 mg TE.g-1 of AA by DPPH method, and 45.16-110.27 mg TE.g-1 of antioxidant activity by phosphomolybdenum method. A very strong correlation found between antioxidant activity by phosphomolybdenum method and TPAC (r = 0.883), TPC (r = 0.858), and TFC (r = 0.843) at the flowering stage. These results can be used for further biochemical and pharmacological investigations of these plants.
Agrobiodiversity for improving nutrition, health and life quality, Dec 1, 2022
The review of previous studies on Paulownia spp. showed that these plants are valuable raw materi... more The review of previous studies on Paulownia spp. showed that these plants are valuable raw materials with polyfunctional use among which are medicinal, forage, energetic, etc. This study demonstrated the accumulation of selected biochemical components in the different parts of Paulownia tomentosa (Thunb.) Steud. genotypes plants by the end of vegetation collected from experimental collections of M. M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine. The accumulation of selected nutrients in the leaves was the following:
Zenodo (CERN European Organization for Nuclear Research), Mar 1, 2019
Objective-to investigate the accumulation of biochemical compounds in the different organs of cul... more Objective-to investigate the accumulation of biochemical compounds in the different organs of cultivars and varieties of Miscanthus Anderss. in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. Investigated plants were cultivars and varieties of species of the genus of Miscanthus collected in an experimental collection of Cultural Flora Department of M.M. Gryshko National Botanical Garden of the NAS of Ukraine: М.
Journal of Native and Alien Plant Studies, 2021
Intr0ducti0n. Because plants are an important factor in the existence of the biosphere and life o... more Intr0ducti0n. Because plants are an important factor in the existence of the biosphere and life on Earth, plant resources are an invaluable asset that requires comprehensive study, conservation and rational use in the context of global climate change. Matcrials and mcth0ds. The subject of research was the collection of spice, essential, medicinal, energy, oilseeds, sugar plants. The object of the study was the process of introduction, selection, conservation and enrichment of genetic resources of energy and aromatic plants. Methods of introduction, acclimatization, adaptation, selection, biotech- nology are used. Physiological, biochemical, allelopathic, and ecological methods were also used. Rcsults and discus- si0n. For many years of introduction and selection work in the department of cultural flora of NBG collected valuable gene pool of useful plants (about 2000 taxa) part of which, namely “Collection Fund of Energy and Aromatic Plants of the National Botanical Garden of the Nat...
Agrobiodiversity for improving nutrition, health and life quality, Nov 1, 2018
The economically important species of genus Brassica L. were investigated in this study. Alcohol ... more The economically important species of genus Brassica L. were investigated in this study. Alcohol and water plant extracts of cultivars and varieties of Brassica campestris and B. rapa were inspected on antiradical activity by DPPH-method (with 2,2-diphenyl-2-picrylhydrazyl). Obtained extracts were measured on a spectrophotometer at wavelength 515 nm. Antiradical activity of methanol extracts was in the range from 34.29 (B. rapa subsp. rapifera Metzger (f. biennis), f. EOTRFO) to 58.09% (B. campestris f. biennis D.C., cv. Oriana). Water extracts demonstrated this activity in range from 58.18 (B. campestris f. biennis D.C., cv. Oriana) to 84.25% (B. campestris f. biennis D.C. × B. rapa L., f. EOTFVS). Antioxidant activity (AA) of extracts of investigated plants was expressed in ascorbic acid equivalent (mg g-1 AAE). The highest AA of methanol extracts was found for B. campestris f. biennis D.C. × B. rapa L., cv. Fitopal (86.07 mg g-1 AAE), the lowest one-for B. rapa subsp. rapifera Metzger (f. biennis), f. EOTRFO (52.78 mg g-1 AAE). Maximal AA of water extracts was registered for B. campestris f. biennis D.C. × B. napus f. biennis D.C., cv. Innovacia (121.77 mg g-1 AAE), minimal-for B. campestris f. biennis D.C. × B. rapa L., f. EOTFVS (85.18 mg g-1 AAE).
Zenodo (CERN European Organization for Nuclear Research), Dec 1, 2017
Conclusions. Obtained data showed that in conditions of M.M. Gryshko National Botanical Garden of... more Conclusions. Obtained data showed that in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine the genus Miscanthus plants is a valuable source of nutrients the most content of which observed in the tillering period. Due to this fact these plants can be recommended as nutritional supplements for animal feed. In addition to the aforementioned content of dry matter, total content of sugars of investigated plants of the genus Miscanthus increased from tillering to seed ripening stage. It is found that content of carotene, ascorbic acid, calcium decreased from tillering to seed ripening stage. The total content of ash decreased to seed ripening phase but not significantly. The level of phosphorus varied during vegetation in investigated plants.
Plant Introduction, Mar 1, 2018
The antioxidant activity of methanol and aqueous extracts based on the discoloration reaction on ... more The antioxidant activity of methanol and aqueous extracts based on the discoloration reaction on the solution of DPPH (2,2-diphenyl-1-picrylhydrazyl free radical) was determined according to Brandt-Williams et al.
Plant varieties studying and protection, May 30, 2022
To conduct a comprehensive study of morphological features of the genus Vigna plants, to assess t... more To conduct a comprehensive study of morphological features of the genus Vigna plants, to assess their productive potential depending on the species characteristics under the conditions of introduction in the Right-Bank Forest-Steppe of Ukraine. Methods. The main method of work was a comparative morphological analysis of plants grown from seeds. The research was carried out at the introduction sites and in the laboratory of the Department of Cultural Flora of the
Acta Scientiarum Polonorum, Mar 30, 2020
Background. Neglected and underutilized plant species could serve as a valuable source of natural... more Background. Neglected and underutilized plant species could serve as a valuable source of natural bioactive compounds. The objective of this study was to evaluate the biological activity of Chinese quince (Pseudocydonia sinensis Schneid) genotypes of Ukrainian and Slovak origin. Materials and methods. The content of the total antioxidant activity (DPPH method and molybdenum reducing antioxidant power), total polyphenol, flavonoid and phenolic acid compounds in the pulp and peel of Chinese quince were compared across five genotypes from Slovakia and three from Ukraine. Results. All tested samples exhibited DPPH • radical scavenging activities with values from 6.17 to 9.56 mg TEAC (Trolox equivalent antioxidant capacity) per gram of dry matter (DM). Antioxidant activity, measured using the molybdenum reducing antioxidant power method, ranged from 69.82 to 225.04 mg TEAC per gram of DM. Total polyphenol content was from 34.73 to 82.02 mg GAE (gallic acid equivalent), while total flavonoid content was from 0.50 to 26.72 mg QE (quercetin equivalent) per gram DM. Phenolic acid content varied from 1.12 to 8.39 mg CAE (caffeic acid equivalent) per gram DM. The peel extracts contained the highest content of bioactive compounds when compared with the pulp extract (from 15.30 to 32.60%). All observed parameters differed significantly between the genotypes. Strong positive correlations (p ≤ 0.05) were observed between the content of phenolic acids and flavonoids in the peel in plants from Slovakia (r = 0.951, r = 0.928, respectively); between the phenolic acid and antioxidant capacities detected using the MRP method-r = 0.950 and r = 0.955 for peel and pulp, respectively; between the determination of antioxidant activity by the DPPH and MRP methods in the peel and pulp in plants from Ukraine (r = 0.986, r = 0.998, respectively). Significantly positive correlations were found between all the parameters in the samples of Ukrainian origin. Conclusion. The results showed that all fruit extracts exhibited strong antioxidant activities, which generally correlated positively with the total phenolic content. This study demonstrates that Chinese quince fruit grown in Ukraine and Slovakia is a perspective source of valuable polyphenol content with high antioxidant activity and is a valuable fruit for use in the agriculture and food industries.
Plant varieties studying and protection, Mar 30, 2023
Ñåëåêö³ÿ òà íàñ³ííèöòâî Селекція елекція та насінництво та насінництво С Селекція елекція та насі... more Ñåëåêö³ÿ òà íàñ³ííèöòâî Селекція елекція та насінництво та насінництво С Селекція елекція та насінництво та насінництво Âñòóï Ïîñòóïîâ³ êë³ìàòè÷í³ çì³íè é àðèäèçàö³ÿ çíà÷íèõ òåðèòîð³é ó âñüîìó ñâ³ò³-íàéãîëîâ-í³ø³ ïðîáëåìè ñüîãîäåííÿ, ùî ïîòðåáóþòü ñâîº÷àñíîãî òà âñåá³÷íîãî âèð³øåííÿ [1-4].
Plant varieties studying and protection, Dec 29, 2021
Ñîðòîâèâ÷åííÿ òà ñîðòîçíàâñòâî Âñòóï Ðîäèíà Fabaceae-ðîñëèíè ³ç âèñîêèì âì³ñòîì ïîaeèâíèõ ðå÷îâèí... more Ñîðòîâèâ÷åííÿ òà ñîðòîçíàâñòâî Âñòóï Ðîäèíà Fabaceae-ðîñëèíè ³ç âèñîêèì âì³ñòîì ïîaeèâíèõ ðå÷îâèí, çîêðåìà ïðîòå¿íó, à òàêîae á³îàêòèâíèìè ô³òîåñòðîãåíàìè, ùî ðîáèòü ¿õ ïåðñïåêòèâíèìè äëÿ âèêîðèñòàííÿ ó ð³çíèõ ñôåðàõ ãîñïîäàðþâàííÿ [1]. Âèäè ðîäó Galega º îäíèìè ³ç íàéö³íí³øèõ é âîäíî÷àñ ìàëîâèâ÷åíèìè ïðåäñòàâíèêàìè ö³º¿ ðîäèíè [2]. Ðîñëèíè âèäó G. orientalis äîñë³äaeóþòüñÿ, çîêðåìà, ÿê êîðìîâ³ [3-7] çàâäÿêè ¿õí³é âè-ñîê³é ïðîäóêòèâíîñò³ [8], ñò³éêîñò³ â ð³çíèõ êë³ìàòè÷íèõ óìîâàõ, åêîíîì³÷í³é ðåíòà
Plant varieties studying and protection, Apr 6, 2021
Purpose. Determine a number of morphometric and biochemical parameters of various genotypes of Bu... more Purpose. Determine a number of morphometric and biochemical parameters of various genotypes of Bunias orientalis L. in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG). Methods. Plant samples of B. orientalis (6 genotypes created in the NBG) were examined during the flowering stage. Determination of dry matter, ash, calcium was carried out according to Hrytsaienko et al. (2003), phosphorus according to Pochinok (1976), sugars, ascorbic acid and lipids were determined according to Krishchenko (1983), β-carotene according to Pleshkov (1985). The energy value of plants was determined using an IKA C-200 calorimeter. The obtained results were analysed statistically. Results. The height of plants varied from 140.9 (Genotype 1) to 157.5 (Genotype 5) cm, stem diameter from 11.67 (Genotype 1) to 16.1 (Genotype 6) mm, the number of internodes from 18.7 (Genotype 1) to 25.7 (Genotype 6), the number of leaves on a stem from 14.11 (Genotype 1) to 21.8 (Genotype 5), leaf lamina length from 14.2 (Genotype 1) to 23.45 (Genotype 6) cm, leaf lamina width from 6.34 (Genotype 1) to 14.5 (Genotype 4) cm, inflorescence length from 27.4 (Genotype 1) to 45.4 (Genotype 3) cm, inflorescence width from 2.32 (Genotype 1) to 4.92 (Genotype 3) cm, and the number of stems from 2.55 (Genotype 2) to 5.33 (Genotype 1). The study of the content of structural and functional compounds and nutrients at the flowering stage showed that the dry matter content was in the range of 13.58-16.00%, sugars 5.07-8.86%, titratable acidity 3.28-4.25%, lipids 3.33-6.61%, ascorbic acid 382.83-693.82 mg%, β-carotene 0.94-3.48 mg%, ash 6.79-9.2%, calcium 1.00-2.44%, phosphorus 1.61-2.67% and energy value 3337.0-3498.0 cal/g. Conclusions. It was revealed that samples of various genotypes of B. orientalis are a valuable source of nutrients at the flowering stage. The biochemical composition of plants depended on the genotype and stage of growth. Results of the morphometric study showed variability of investigated parameters. The obtained data can be used to predict and evaluate the results of introduction and breeding studies with B. orientalis genotypes as promising crops in Ukraine.
Plant varieties studying and protection, Jul 15, 2019
Ô³ç³îëîã³ÿ ðîñëèí Âñòóï Çáåðåaeåííÿ, çáàãà÷åííÿ òà åôåêòèâíå âèêîðèñòàííÿ ðîñëèííèõ ðåñóðñ³â ìຠ... more Ô³ç³îëîã³ÿ ðîñëèí Âñòóï Çáåðåaeåííÿ, çáàãà÷åííÿ òà åôåêòèâíå âèêîðèñòàííÿ ðîñëèííèõ ðåñóðñ³â ìຠâàaeëèâå íàóêîâå òà ïðàêòè÷íå çíà÷åííÿ. Çâàaeàþ÷è íà öå, ³íòðîäóêö³ÿ íîâèõ íåòðàäèö³éíèõ ìàëîïîøèðåíèõ âèä³â ðîñëèí ³ ðîçðîáëÿííÿ á³î-ëîã³÷íèõ, á³îõ³ì³÷íèõ, á³îòåõíîëîã³÷íèõ îñíîâ ââåäåííÿ ¿õ ó ïðîìèñëîâó òà àìàòîðñüêó êóëüòóðó º íàäçâè÷àéíî âàaeëèâèì çàâäàííÿì. Äî ïåðñïåêòèâíèõ äëÿ ³íòðîäóêö³¿ âèä³â ðîñëèí íàëåaeàòü ïðåäñòàâíèêè ðîäó Serratula L., ùî íàðàõîâóº ïðèáëèçíî 70 âèä³â, ïîøèðåíèõ ó ªâðà糿 ³ ϳâí³÷í³é Àôðèö³ [1].  Óê-ðà¿í³ òðàïëÿþòüñÿ ï'ÿòü âèä³â ðîäó Serratula [2]. Âèäè ðîäó Serratula L., íàñàìïåðåä Serratula coronata L., â³äçíà÷àþòüñÿ âèñîêî-ö³ííîþ ô³òîñèðîâèíîþ, à òîìó º ïåðñïåêòèâíèìè äëÿ âèêîðèñòàííÿ â ìåäèöèí³ ÷åðåç âè-ÿâëåí³ â íèõ á³îëîã³÷íî àêòèâí³ ðå÷îâèíè-ô³òîåêäèñòåðî¿äè. Âèâ÷åííÿ á³îõ³ì³÷íèõ îñî
Zenodo (CERN European Organization for Nuclear Research), Aug 1, 2017
Objective-to establish biological and biochemical properties of plants of the genus Agrostis L. s... more Objective-to establish biological and biochemical properties of plants of the genus Agrostis L. species in conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Material and methods. The objects of these investigations were species and cultivars of the genus Agrostis species. It was conducted morphometric measurements and some parameters of the productivity in the flowering stage.
Ìntrodukcìâ roslin, Apr 28, 2022
Cosmos sulphureus is an adventitious species for Europe and Ukraine in particular. It originates ... more Cosmos sulphureus is an adventitious species for Europe and Ukraine in particular. It originates from Central and South America, where it grows in tropical and subtropical climates. The climatic conditions of Kyiv are characterized as temperate, with an absolute temperature minimum of-32.2 °C, an absolute maximum of +39.9 °C, and average annual rainfall of 649 mm. Plants were analyzed in the flowering phase, divided into inflorescences, leaves, stems, and roots, dried at +35 °C and then extracted with methanol and water. The determination of the antiradical activity was carried out according to a modified method using a DPPH (2,2-diphenyl-1-picrylhydrazyl) radical inhibition reaction. The highest antiradical activity was detected in inflorescences (59.60-81.81 % inhibition) and leaves (79.81-89.12 % inhibition). Stem extracts had an average level of inhibition (19.63-65.93 %), and root extracts showed only 2.54-39.46 % inhibition. Correlation analysis showed a strong relationship between leaves and stems (r = 0.84), leaves and roots (r = 0.81), and stems and roots (r = 0.91). Extracts of C. sulphureus plants grown in temperate climate were found having a high antioxidant potential but lower than that reported for tropical and subtropical regions. It was found that higher intensity of coloration of marginal florets of the capitulum does not correlate with a higher antiradical activity. Methanolic and water extracts of inflorescences of the genotype CSCO-368812 with intensilly colored perianth inhibited only 59.60 % and 71.50 % of radicals, while similar extracts of the genotype CS-361294 with lighter florets inhibited 71.17 % and 81.81 % of radicals, respectively. Instead, there was a difference in antiradical activity depending on applied extractant. Methanolic extracts of vegetative organs (leaves, stems, roots) prevail over water extracts in terms of their antiradical activity. However, water extracts of inflorescences of both genotypes demonstrated higher level of antiradical activity.
Acta Scientiarum Polonorum, Jun 30, 2018
Background. American persimmons (Diospyros virginiana L.) are known as a widespread cultivar whic... more Background. American persimmons (Diospyros virginiana L.) are known as a widespread cultivar which were traditionally used by Native Americans as a food source, and since ancient times have been used in folk medicine. The objective of this study was to evaluate the antioxidant activity and phenolic content of Diospyros virginiana genotypes. Material and methods. The content of the total antioxidant activity and phenolic compounds from the fruits of the American persimmon (Diospyros virginiana L.) of six genotypes were compared. Antioxidant activity (AOA) was measured using three different photometric methods-DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) and FRAP (ferric-reducing antioxidant power). Total phenolic content (TPC) was evaluated using Folin-Ciocalteu reagent assay. Results. The results for AOA (μMol Trolox/g) determined by the DPPH method varied from 51.68 (DV-05) to 100.87 (DV-03), those obtained by the ABTS method varied from 65.40 (DV-05) to 142.26 (DV-03), and those obtained by the FRAP method varied from 45.06 (DV-05) to 109.30 (DV-03). The results for TPC varied from 590.75 ±27.98 mg/100 g (DV-05) to 1325.12 ±77.30 mg/100 g (DV-03). The highest results for AOA and TPC were achieved for the fruits of genotypes DV-03 and DV-05. A positive linear correlation was found between antioxidant activity and total phenolic content in the examined plant material. Conclusion. The results showed that all fruit extracts exhibited strong antioxidant activities, which generally correlated positively with the total phenolic content. This study demonstrates the potential of the fruits of Diospyros virginiana grown in Ukraine as a possible source of valuable polyphenol content, with high antioxidant activities and health-promoting properties. The high contents of phenolic compounds and significant linear correlation between the values of the concentration of phenolic compounds and antioxidant activity indicated that these compounds contributed to the strong antioxidant activity.
Plant varieties studying and protection, Jan 31, 2023
Plant physiology Фізіологія рослин ізіологія рослин Ф Фізіологія рослин ізіологія рослин Introduc... more Plant physiology Фізіологія рослин ізіологія рослин Ф Фізіологія рослин ізіологія рослин Introduction The biochemical analysis of both introduced and well-known medicinal and aromatic plants is an important task [1, 2]. This is because of