Filipe Olim - Academia.edu (original) (raw)

Papers by Filipe Olim

European Journal of Lipid Science and Technology, 2021

Developing new easy-to-prepare functional drug delivery nanosystems with good storage stability, ... more Developing new easy-to-prepare functional drug delivery nanosystems with good storage stability, low hemotoxicity, as well as controllable drug delivery property, has attracted great attention in recent years. In this work, a cholesterol-based prodrug nanodelivery system is prepared by self-assembly of cholesterol-doxorubicin prodrug conjugates (Chol-Dox) and tocopherol polyethylene glycol succinate (TPGS) using thin-film hydration method. The Chol-Dox/TPGS assemblies (molar ratio 2:1, 1:1, and 1:2) are able to form nanoparticles with average hydrodynamic diameter of ≈140-214 nm, surface zeta potentials of ≈−24.2-−0.3 mV, and remarkable solution stability in 0.1 m PBS, 16 days). The Chol-Dox/TPGS assemblies show low hemotoxicity and different cytotoxicity profiles in breast cancer cells (MCF-7 and MDA-MB-231), which are largely dependent on the molar ratio of Chol-Dox and TPGS. The Chol-Dox/TPGS assemblies tend to enter into MCF-7 and MDA-MB-231 cells through non-Clathrin-mediated multiple endocytosis and lysosome-dependent uptake pathways, moreover, these nanoassemblies demonstrate lysosome-dependent intracellular localization, which is different from that of free DOX (nuclear localization). The results demonstrate that the Chol-Dox/TPGS assemblies are promising cholesterol-based prodrug nanomaterials for breast cancer chemotherapy. Practical Applications: This work demonstrates a lipid prodrug-based nanotherapeutic system. Herein the Chol-Dox/TPGS nanoassemblies could serve as promising and controllable cholesterol-based prodrug nanomaterials/nano-formulations for potential breast cancer chemotherapy.

General Chemistry

Harnessing natural-based renewable molecular resources to construct functional synthetic green po... more Harnessing natural-based renewable molecular resources to construct functional synthetic green polymers is a promising research frontier at the interface of sustainable/green chemistry, polymer chemistry and nanobiotechnology. As natural glycoprotein mimics/analogues and biocompatible building blocks of nanobiomaterials, synthetic functional glycopolypeptides and their structural/functional analogues have attracted great attentions in recent years. This mini-perspective article reviewed current synthetic strategies and methods of glycopolypeptides and their analogues. The pros and cons of the synthesis protocols were discussed, moreover, possible future perspectives in this field were also stated.

International Studies in Entrepreneurship, 2014

The literature has shown that a high level of new firm formation contributes to a region's econom... more The literature has shown that a high level of new firm formation contributes to a region's economic performance and is a signal of a thriving economy built on innovation. Amongst the many factors promoting new firm formation, such as market size, industrial structure or human capital, creativity has been neglected for some time. Richard Florida's The Rise of the Creative Class (2002) was a seminal contribution for the recognition of the importance of creative people, creative industries, creative economies and, consequently, creativity. Many authors, inspired by this contribution, have been undertaking theoretical and empirical studies to analyse the role of creativity in economics. The purpose of this thesis is to follow such contributions, discussing the relationship between entrepreneurship and creativity in a particular, relatively peripheral country: Portugal. A multivariate linear regression analysis is applied, explaining new firm formation across Portuguese regions with explanatory variables that include both creativity and diversity indexes, innovation indicators and the human capital dimension, along with control variables. Our results show little evidence of the influence of creativity on the birth of new firms in Portugal, while pointing to the relevance of agglomeration effects for new firm formation. Additionally, they suggest that immigrants might be facing difficulties in establishing a firm in Portugal, which may be explained by language barriers, financing difficulties and excessive bureaucracy.

European Journal of Lipid Science and Technology, 2021

Developing new easy-to-prepare functional drug delivery nanosystems with good storage stability, ... more Developing new easy-to-prepare functional drug delivery nanosystems with good storage stability, low hemotoxicity, as well as controllable drug delivery property, has attracted great attention in recent years. In this work, a cholesterol-based prodrug nanodelivery system is prepared by self-assembly of cholesterol-doxorubicin prodrug conjugates (Chol-Dox) and tocopherol polyethylene glycol succinate (TPGS) using thin-film hydration method. The Chol-Dox/TPGS assemblies (molar ratio 2:1, 1:1, and 1:2) are able to form nanoparticles with average hydrodynamic diameter of ≈140-214 nm, surface zeta potentials of ≈−24.2-−0.3 mV, and remarkable solution stability in 0.1 m PBS, 16 days). The Chol-Dox/TPGS assemblies show low hemotoxicity and different cytotoxicity profiles in breast cancer cells (MCF-7 and MDA-MB-231), which are largely dependent on the molar ratio of Chol-Dox and TPGS. The Chol-Dox/TPGS assemblies tend to enter into MCF-7 and MDA-MB-231 cells through non-Clathrin-mediated multiple endocytosis and lysosome-dependent uptake pathways, moreover, these nanoassemblies demonstrate lysosome-dependent intracellular localization, which is different from that of free DOX (nuclear localization). The results demonstrate that the Chol-Dox/TPGS assemblies are promising cholesterol-based prodrug nanomaterials for breast cancer chemotherapy. Practical Applications: This work demonstrates a lipid prodrug-based nanotherapeutic system. Herein the Chol-Dox/TPGS nanoassemblies could serve as promising and controllable cholesterol-based prodrug nanomaterials/nano-formulations for potential breast cancer chemotherapy.

General Chemistry

Harnessing natural-based renewable molecular resources to construct functional synthetic green po... more Harnessing natural-based renewable molecular resources to construct functional synthetic green polymers is a promising research frontier at the interface of sustainable/green chemistry, polymer chemistry and nanobiotechnology. As natural glycoprotein mimics/analogues and biocompatible building blocks of nanobiomaterials, synthetic functional glycopolypeptides and their structural/functional analogues have attracted great attentions in recent years. This mini-perspective article reviewed current synthetic strategies and methods of glycopolypeptides and their analogues. The pros and cons of the synthesis protocols were discussed, moreover, possible future perspectives in this field were also stated.

International Studies in Entrepreneurship, 2014

The literature has shown that a high level of new firm formation contributes to a region's econom... more The literature has shown that a high level of new firm formation contributes to a region's economic performance and is a signal of a thriving economy built on innovation. Amongst the many factors promoting new firm formation, such as market size, industrial structure or human capital, creativity has been neglected for some time. Richard Florida's The Rise of the Creative Class (2002) was a seminal contribution for the recognition of the importance of creative people, creative industries, creative economies and, consequently, creativity. Many authors, inspired by this contribution, have been undertaking theoretical and empirical studies to analyse the role of creativity in economics. The purpose of this thesis is to follow such contributions, discussing the relationship between entrepreneurship and creativity in a particular, relatively peripheral country: Portugal. A multivariate linear regression analysis is applied, explaining new firm formation across Portuguese regions with explanatory variables that include both creativity and diversity indexes, innovation indicators and the human capital dimension, along with control variables. Our results show little evidence of the influence of creativity on the birth of new firms in Portugal, while pointing to the relevance of agglomeration effects for new firm formation. Additionally, they suggest that immigrants might be facing difficulties in establishing a firm in Portugal, which may be explained by language barriers, financing difficulties and excessive bureaucracy.