Onyeka Michael Ikele - Academia.edu (original) (raw)
articles by Onyeka Michael Ikele
Journal of World’s Poultry Research, 2021
The current study aimed to evaluate whether the probiotic Lactobacillus casei could be effective ... more The current study aimed to evaluate whether the probiotic Lactobacillus casei could be effective in controlling chicken intestinal colibacillosis. Avian pathogenic Escherichia coli (E. coli.) and Lactobacillus casei (L. casei) isolates were obtained from nono (a sour milk product produced by Fulani tribe of Nigeria), and were used for the chicken infection and probiotic treatment, respectively. The experimental design was conducted on three-week-old broiler chicks, which were divided into five groups, namely A (healthy control), B (infected without treatment), C (infected and treated with antibiotic), D (infected and treated with L. casei), and E (initially given L. casei before infecting with E. coli). Groups C and D were treated using 15 g/L norfloxacin and 1.5 ml of 1.1x10 9 cfu/ml L. casei, respectively. Group E was given the oral infusion of 1.5 ml of 1.1 × 10 9 cfu/ml L. casei before infection with 1.5 ml of 1.3 × 10 7 cfu/ml avian pathogenic E. coli. Weight, hematological parameters, liver function, and fecal E. coli counts of the chicks were monitored and used to evaluate the level of protection elicited by the probiotic organism. There was weight gain in chicken groups, except for group B. There was a significant difference in the sodium, chlorine, and bicarbonate levels amongst the groups. The hematological profile revealed a significant difference in the hemoglobin, white blood cells, lymphocyte, and neutrophil counts of the chicken groups. Assessment of liver enzymes showed no significant difference amongst the chick groups except in group B. Similar results were obtained for the urea, creatinine, and C-reactive protein levels. The microbial tests revealed a decrease in the total E. coli count for groups C, D, and E. The results of the current study indicated that L. casei could be used as a probiotic in the control of chicken colibacillosis.
Papers by Onyeka Michael Ikele
Journal of pure and applied microbiology, May 31, 2024
Fingerlings of the African catfish, C. gariepinus (mean weight 13.13±2.77g) were exposed to linea... more Fingerlings of the African catfish, C. gariepinus (mean weight 13.13±2.77g) were exposed to linear alkyl benzene sulphonate (0.0166mg/L) under laboratory conditions. The tissues (liver, gill, muscle and kidney) enzyme parameters were analysed after 0hr (initial) 2hr, 12hr and 24h exposure. The increased neutral and alkaline proteases activities in the different tissues of C. gariepinus indicates the damage caused due to impairment of energy supply and alkaline proteases activity indicates higher protein degradation. Therefore, the proteins are denatured leading to more activation of protease. During the toxic exposure of LAS, the aspartate aminotransfarase (AAT) and alaninetransfarase (ALAT) were enhanced which led to incorporation of amino acids into TCA cycle for energy production. Whereas, glutamate dehydrogenase activity similarly enhanced the elevated level of amino acids, transaminases which possibly suggest the utilization of protein under stress condition of fish. Possible Impaired respiration was evident due to the toxic insult of LAS to the gill which showed severe damage such as filaments with disjointed lamella. In the liver, there was mild vacuolation and congestion of the liver tissue. The kidney showed sequensiation of the kidney architecture with accumulation of hyaline droplets in the tubular epithelial cell. These changes occur predominantly in the 24hr exposure. LAS is toxic to fingerlings fish C. gariepinus are more susceptible to LAS, therefore their use on/near fish farm or in area close to aquatic environment should be discouraged.
Journal of Animal Health and Production, 2020
A ntibiotic resistance has been a global threat to disease treatment and this extends from humans... more A ntibiotic resistance has been a global threat to disease treatment and this extends from humans to food animals. Alternatives to antibiotic treatments are being investigated in order to control antibiotic resistance rate in bacteria and one of such alternatives is the use of prebiotics. Prebiotics are non-digestible substances that provide beneficial physiological effect on the host when consumed, by selectively stimulating the favourable growth or activity of beneficial bacteria such as Lactobacilli and
Journal of World’s Poultry Research, 2021
The current study aimed to evaluate whether the probiotic Lactobacillus casei could be effective ... more The current study aimed to evaluate whether the probiotic Lactobacillus casei could be effective in controlling chicken intestinal colibacillosis. Avian pathogenic Escherichia coli (E. coli.) and Lactobacillus casei (L. casei) isolates were obtained from nono (a sour milk product produced by Fulani tribe of Nigeria), and were used for the chicken infection and probiotic treatment, respectively. The experimental design was conducted on three-week-old broiler chicks, which were divided into five groups, namely A (healthy control), B (infected without treatment), C (infected and treated with antibiotic), D (infected and treated with L. casei), and E (initially given L. casei before infecting with E. coli). Groups C and D were treated using 15 g/L norfloxacin and 1.5 ml of 1.1x10 9 cfu/ml L. casei, respectively. Group E was given the oral infusion of 1.5 ml of 1.1 × 10 9 cfu/ml L. casei before infection with 1.5 ml of 1.3 × 10 7 cfu/ml avian pathogenic E. coli. Weight, hematological parameters, liver function, and fecal E. coli counts of the chicks were monitored and used to evaluate the level of protection elicited by the probiotic organism. There was weight gain in chicken groups, except for group B. There was a significant difference in the sodium, chlorine, and bicarbonate levels amongst the groups. The hematological profile revealed a significant difference in the hemoglobin, white blood cells, lymphocyte, and neutrophil counts of the chicken groups. Assessment of liver enzymes showed no significant difference amongst the chick groups except in group B. Similar results were obtained for the urea, creatinine, and C-reactive protein levels. The microbial tests revealed a decrease in the total E. coli count for groups C, D, and E. The results of the current study indicated that L. casei could be used as a probiotic in the control of chicken colibacillosis.
Journal of pure and applied microbiology, May 31, 2024
Fingerlings of the African catfish, C. gariepinus (mean weight 13.13±2.77g) were exposed to linea... more Fingerlings of the African catfish, C. gariepinus (mean weight 13.13±2.77g) were exposed to linear alkyl benzene sulphonate (0.0166mg/L) under laboratory conditions. The tissues (liver, gill, muscle and kidney) enzyme parameters were analysed after 0hr (initial) 2hr, 12hr and 24h exposure. The increased neutral and alkaline proteases activities in the different tissues of C. gariepinus indicates the damage caused due to impairment of energy supply and alkaline proteases activity indicates higher protein degradation. Therefore, the proteins are denatured leading to more activation of protease. During the toxic exposure of LAS, the aspartate aminotransfarase (AAT) and alaninetransfarase (ALAT) were enhanced which led to incorporation of amino acids into TCA cycle for energy production. Whereas, glutamate dehydrogenase activity similarly enhanced the elevated level of amino acids, transaminases which possibly suggest the utilization of protein under stress condition of fish. Possible Impaired respiration was evident due to the toxic insult of LAS to the gill which showed severe damage such as filaments with disjointed lamella. In the liver, there was mild vacuolation and congestion of the liver tissue. The kidney showed sequensiation of the kidney architecture with accumulation of hyaline droplets in the tubular epithelial cell. These changes occur predominantly in the 24hr exposure. LAS is toxic to fingerlings fish C. gariepinus are more susceptible to LAS, therefore their use on/near fish farm or in area close to aquatic environment should be discouraged.
Journal of Animal Health and Production, 2020
A ntibiotic resistance has been a global threat to disease treatment and this extends from humans... more A ntibiotic resistance has been a global threat to disease treatment and this extends from humans to food animals. Alternatives to antibiotic treatments are being investigated in order to control antibiotic resistance rate in bacteria and one of such alternatives is the use of prebiotics. Prebiotics are non-digestible substances that provide beneficial physiological effect on the host when consumed, by selectively stimulating the favourable growth or activity of beneficial bacteria such as Lactobacilli and