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Oscar Alen

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Papers by Oscar Alen

Research paper thumbnail of Functional Analysis of Promoters in the Nisin Gene Cluster of Lactococcus lactis

The promoters in the nisin gene cluster nisABTCIPRKFEG of Lactococcus lactis were characterized b... more The promoters in the nisin gene cluster nisABTCIPRKFEG of Lactococcus lactis were characterized by primer extension and transcriptional fusions to the Escherichia coli promoterless ␤-glucuronidase gene (gusA). Three promoters preceding the nisA, nisR, and nisF genes, which all give rise to gusA expression in the nisin-producing strain L. lactis NZ9700, were identified. The transcriptional autoregulation of nisA by signal transduction involving the sensor histidine kinase NisK and the response regulator NisR has been demonstrated previously (O. P. Kuipers, M. M. Beerthuyzen, P. G. G. A. de Ruyter, E. J. Luesink, and W. M. de Vos, J. Biol. Chem. 270: 27299-27304, 1995)

Research paper thumbnail of ENZIMASHoloenzima = Apoenzima + Coenzima Activadores Metálicos

El diagrama de cintas de la enzima TIM, rodeado por el modelo de relleno de espacio de la proteín... more El diagrama de cintas de la enzima TIM, rodeado por el modelo de relleno de espacio de la proteína. La TIM es una enzima extremadamente eficiente envuelta en el proceso que convierte azúcar a energía en el cuerpo.

Research paper thumbnail of Functional Analysis of Promoters in the Nisin Gene Cluster of Lactococcus lactis

The promoters in the nisin gene cluster nisABTCIPRKFEG of Lactococcus lactis were characterized b... more The promoters in the nisin gene cluster nisABTCIPRKFEG of Lactococcus lactis were characterized by primer extension and transcriptional fusions to the Escherichia coli promoterless ␤-glucuronidase gene (gusA). Three promoters preceding the nisA, nisR, and nisF genes, which all give rise to gusA expression in the nisin-producing strain L. lactis NZ9700, were identified. The transcriptional autoregulation of nisA by signal transduction involving the sensor histidine kinase NisK and the response regulator NisR has been demonstrated previously (O. P. Kuipers, M. M. Beerthuyzen, P. G. G. A. de Ruyter, E. J. Luesink, and W. M. de Vos, J. Biol. Chem. 270: 27299-27304, 1995)

Research paper thumbnail of ENZIMASHoloenzima = Apoenzima + Coenzima Activadores Metálicos

El diagrama de cintas de la enzima TIM, rodeado por el modelo de relleno de espacio de la proteín... more El diagrama de cintas de la enzima TIM, rodeado por el modelo de relleno de espacio de la proteína. La TIM es una enzima extremadamente eficiente envuelta en el proceso que convierte azúcar a energía en el cuerpo.

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