Osman Radwan - Academia.edu (original) (raw)
Papers by Osman Radwan
International Biodeterioration & Biodegradation
Qatar Foundation Annual Research Conference Proceedings Volume 2016 Issue 1, 2016
Microbiology resource announcements, Dec 2, 2021
Microbiology Resource Announcements, 2021
We report the metagenome-assembled genomes (MAGs) of 12 different bacterial species recovered fro... more We report the metagenome-assembled genomes (MAGs) of 12 different bacterial species recovered from environmental microbiomes associated with biofouled plastic fabrics. The MAGs have estimated sizes of 2.53 to 7.66 Mb with 3,229 to 9,289 proteins, 26.20% to 99.1% genome completeness, 48.9% to 72.6% G+C content, and multiple genes for hydrocarbon degradation.
Microbiology Resource Announcements, 2021
We report the assembly and annotation of 10 different black yeast genomes from microbiome metagen... more We report the assembly and annotation of 10 different black yeast genomes from microbiome metagenomic data derived from biofouled plastic fabrics. The draft genomes are estimated to be 9 to 33.2 Mb, with 357 to 5,108 contigs and G+C contents of 43.9% to 57.4%, and they harbor multiple genes for hydrocarbon adaptation and degradation.
Raw data of GC-MS profile of hydrocarbons degraded by Pseudomonas frederiksbergensis SI8 grown fo... more Raw data of GC-MS profile of hydrocarbons degraded by Pseudomonas frederiksbergensis SI8 grown for 14 days at 4ºC in Jet A fuel.
Raw data of degradation level for seventeen aromatic hydrocarbons after exposure of Jet A fuel to... more Raw data of degradation level for seventeen aromatic hydrocarbons after exposure of Jet A fuel to P. frederiksbergensis SI8 for 14 days at 4ºC and 28°C. SD, standard deviation of n=3.
14-3-3 proteins are important components in signal transduction pathways affecting multiple plant... more 14-3-3 proteins are important components in signal transduction pathways affecting multiple plant functions by mediating protein-protein interactions through post-translational modification via phosphorylation. Homodimers or heterodimers of 14-3-3 proteins bind to other proteins inducing site-specific targeting and alteration of the conformation of client proteins, which is an essential regulatory element for plant function and response. This protein family is involved in a wide range of cellular functions including the regulation of hormonal induction in response to stress stimuli, mediation of symbiotic relationships between the plant host and symbionts, as well as the mediation of functions between cellular components and enzymes. Identification of the function and mechanism of 14-3-3 proteins in plants offers insight into how crops can be made more efficient via the manipulation of these proteins.
Soybean GM-RLK3 is a receptor-like kinase gene, which shares high similarity with the Arabidopsis... more Soybean GM-RLK3 is a receptor-like kinase gene, which shares high similarity with the Arabidopsis thaliana BAM family gene suggesting a similar functional role in promoting meristem size and cell differentiation. While the functional role of RLK3 has been heavily investigated in models as well as in some agronomic crops, no studies have been conducted in soybean. The aim of this study was to examine the functional role of GM-RLK3 in soybean growth and development, in addition to assessing the response of transgenic soybean to biotic and abiotic stresses under greenhouse conditions. Stable transgenic plants for overexpression of GM-RLK3 were developed using immature cotyledon explants. Real time-PCR demonstrated the overexpression of this transgene in different tissues, and showed that GM-RLK3 was overexpressed in unifoliate tissues (52 fold increase) with lower expression in pod tissues (7.7 fold increase). Results showed that overexpression of GM-RLK3 did not improve soybean resist...
Raw GC-MS data for the hydrocarbon degradation profile of Pseudomonas frederiksbergensis SI8 grow... more Raw GC-MS data for the hydrocarbon degradation profile of Pseudomonas frederiksbergensis SI8 grown for 14 days at 4ºC in Jet A fuel. Control, did not contain bacteria and was measured after 14 days. D02, day 2 samples with bacteria (n=3); D04, day 4 samples with bacteria (n=3); D07, day 7 samples with bacteria (n=1); D14, day 14 samples with bacteria (n=3).
International Biodeterioration & Biodegradation, 2020
Abstract Biodeterioration of plasticized fabrics is a serious problem leading to degradation of m... more Abstract Biodeterioration of plasticized fabrics is a serious problem leading to degradation of materials used in military and civilian applications. This study aimed to characterize the composition of the microbial communities present on six plasticized fabrics exposed to a harsh tropical environment and explore their role in biodeterioration. Metagenomics, bioinformatics, light and scanning electron microscopy (SEM), and solid-phase microextraction GC-MS were used to characterize the fabric-associated microbial communities and plasticizer degradation. SEM analysis showed multi-layered biofilms containing bacteria and a high abundance of fungal structures and yeast cells. Shotgun metagenomics with a multifaceted bioinformatic pipeline generated 3,314,688 contigs and 120 microbial genomes. The microbial genomes were classified into three domains with the majority belonging to fungi followed by bacteria, and archaea. Functional gene annotation revealed that the fabric-associated microbiomes harbor important pathways for energy generation, stress tolerance, and compounds degradation including esterases, lipases and monooxygenases, suggesting a role of these microorganisms in degradation. The results showed that black yeasts were the keystone species of microbiomes affecting the fabrics. Information from this study will help to understand the effect of different microbial communities and species on the biodeterioration of fabrics and may help support the development of new antimicrobial materials.
Molecular Biotechnology, 2020
International Biodeterioration & Biodegradation
Qatar Foundation Annual Research Conference Proceedings Volume 2016 Issue 1, 2016
Microbiology resource announcements, Dec 2, 2021
Microbiology Resource Announcements, 2021
We report the metagenome-assembled genomes (MAGs) of 12 different bacterial species recovered fro... more We report the metagenome-assembled genomes (MAGs) of 12 different bacterial species recovered from environmental microbiomes associated with biofouled plastic fabrics. The MAGs have estimated sizes of 2.53 to 7.66 Mb with 3,229 to 9,289 proteins, 26.20% to 99.1% genome completeness, 48.9% to 72.6% G+C content, and multiple genes for hydrocarbon degradation.
Microbiology Resource Announcements, 2021
We report the assembly and annotation of 10 different black yeast genomes from microbiome metagen... more We report the assembly and annotation of 10 different black yeast genomes from microbiome metagenomic data derived from biofouled plastic fabrics. The draft genomes are estimated to be 9 to 33.2 Mb, with 357 to 5,108 contigs and G+C contents of 43.9% to 57.4%, and they harbor multiple genes for hydrocarbon adaptation and degradation.
Raw data of GC-MS profile of hydrocarbons degraded by Pseudomonas frederiksbergensis SI8 grown fo... more Raw data of GC-MS profile of hydrocarbons degraded by Pseudomonas frederiksbergensis SI8 grown for 14 days at 4ºC in Jet A fuel.
Raw data of degradation level for seventeen aromatic hydrocarbons after exposure of Jet A fuel to... more Raw data of degradation level for seventeen aromatic hydrocarbons after exposure of Jet A fuel to P. frederiksbergensis SI8 for 14 days at 4ºC and 28°C. SD, standard deviation of n=3.
14-3-3 proteins are important components in signal transduction pathways affecting multiple plant... more 14-3-3 proteins are important components in signal transduction pathways affecting multiple plant functions by mediating protein-protein interactions through post-translational modification via phosphorylation. Homodimers or heterodimers of 14-3-3 proteins bind to other proteins inducing site-specific targeting and alteration of the conformation of client proteins, which is an essential regulatory element for plant function and response. This protein family is involved in a wide range of cellular functions including the regulation of hormonal induction in response to stress stimuli, mediation of symbiotic relationships between the plant host and symbionts, as well as the mediation of functions between cellular components and enzymes. Identification of the function and mechanism of 14-3-3 proteins in plants offers insight into how crops can be made more efficient via the manipulation of these proteins.
Soybean GM-RLK3 is a receptor-like kinase gene, which shares high similarity with the Arabidopsis... more Soybean GM-RLK3 is a receptor-like kinase gene, which shares high similarity with the Arabidopsis thaliana BAM family gene suggesting a similar functional role in promoting meristem size and cell differentiation. While the functional role of RLK3 has been heavily investigated in models as well as in some agronomic crops, no studies have been conducted in soybean. The aim of this study was to examine the functional role of GM-RLK3 in soybean growth and development, in addition to assessing the response of transgenic soybean to biotic and abiotic stresses under greenhouse conditions. Stable transgenic plants for overexpression of GM-RLK3 were developed using immature cotyledon explants. Real time-PCR demonstrated the overexpression of this transgene in different tissues, and showed that GM-RLK3 was overexpressed in unifoliate tissues (52 fold increase) with lower expression in pod tissues (7.7 fold increase). Results showed that overexpression of GM-RLK3 did not improve soybean resist...
Raw GC-MS data for the hydrocarbon degradation profile of Pseudomonas frederiksbergensis SI8 grow... more Raw GC-MS data for the hydrocarbon degradation profile of Pseudomonas frederiksbergensis SI8 grown for 14 days at 4ºC in Jet A fuel. Control, did not contain bacteria and was measured after 14 days. D02, day 2 samples with bacteria (n=3); D04, day 4 samples with bacteria (n=3); D07, day 7 samples with bacteria (n=1); D14, day 14 samples with bacteria (n=3).
International Biodeterioration & Biodegradation, 2020
Abstract Biodeterioration of plasticized fabrics is a serious problem leading to degradation of m... more Abstract Biodeterioration of plasticized fabrics is a serious problem leading to degradation of materials used in military and civilian applications. This study aimed to characterize the composition of the microbial communities present on six plasticized fabrics exposed to a harsh tropical environment and explore their role in biodeterioration. Metagenomics, bioinformatics, light and scanning electron microscopy (SEM), and solid-phase microextraction GC-MS were used to characterize the fabric-associated microbial communities and plasticizer degradation. SEM analysis showed multi-layered biofilms containing bacteria and a high abundance of fungal structures and yeast cells. Shotgun metagenomics with a multifaceted bioinformatic pipeline generated 3,314,688 contigs and 120 microbial genomes. The microbial genomes were classified into three domains with the majority belonging to fungi followed by bacteria, and archaea. Functional gene annotation revealed that the fabric-associated microbiomes harbor important pathways for energy generation, stress tolerance, and compounds degradation including esterases, lipases and monooxygenases, suggesting a role of these microorganisms in degradation. The results showed that black yeasts were the keystone species of microbiomes affecting the fabrics. Information from this study will help to understand the effect of different microbial communities and species on the biodeterioration of fabrics and may help support the development of new antimicrobial materials.
Molecular Biotechnology, 2020