Christian Oste - Academia.edu (original) (raw)
Papers by Christian Oste
Journal of Tissue Science and Engineering, Jul 31, 2015
Archives internationales de physiologie et de biochimie, 1977
Doctorat en Sciencesinfo:eu-repo/semantics/nonPublishe
筑波大学倫理学原論研究会, Dec 20, 2001
한반도 남동부에서 어일분지내의 마이오세 화산암류는 성분상 규장질(67.2-70.5wt.% SiO₂)과 염기성 (49.3-55.2wt.% SiO₂)으로 구성되는 쌍모식 조성을 ... more 한반도 남동부에서 어일분지내의 마이오세 화산암류는 성분상 규장질(67.2-70.5wt.% SiO₂)과 염기성 (49.3-55.2wt.% SiO₂)으로 구성되는 쌍모식 조성을 나타낸다. 이 분지에서의 쌍모식 화산작용은 분지 발달과 정과 밀접하게 연관되어 있으며, 화산암류는 분지내의 두터운 퇴적암층내에 협재되어 있으며, 특징적으로 반정 광물조합의 비평형을 나타낸다. 현무암질 용암에서는 융식된 석영 반정과 공존하는 감람석과 사방휘석 반정이 산출되고, 데사이트~유문암질 용결회류응회암에서는 사방휘석과 단사휘석이 반정으로 출현한다. 구성 광물상의 불균형으로부터 어일분지에서 일어난 쌍모식 화산활동에서 서로 다른 성분의 마그마 혼합이 크게 작용하였음을 시사해준다.
MGG Molecular & General Genetics, 1977
A new photoactivable reagent is described, which allows the formation of RNA-protein cross-links ... more A new photoactivable reagent is described, which allows the formation of RNA-protein cross-links via disulfide bridges in combination with mercaptobutyrimidate. The reconstituted L24 protein-23S RNA complex from the large subunit of E. coli ribosomes has been used as a model system for the cross-linking. The main advantages of the reagent are the absence of U.V. generated cross-links, since photoactivation is carried out at 360 nm, on one hand and the ease of cleavage of the cross-link by mild reduction (beta-mercaptoethanol) on the other.
Journal of Food Protection, 1995
This study evaluates a polymerase chain-reaction assay coupled with fluorescence detection (PCR-F... more This study evaluates a polymerase chain-reaction assay coupled with fluorescence detection (PCR-FD) in microwell plates for Listeria monocytogenes in dairy and food samples. Guanidinium thiocyanate/silica–extracted cultures and milk and dairy-food samples were used as templates for a PCR assay in microwell plates, with a primer pair that amplifies a 221 bp segment of the internal transcribed spacer (ITS), in the presence of 0.5 μg/ml of ethidium bromide. Fluorescence of the PCR products was measured with a CytoFluor 2300 fluorescence measurement system (Millipore Corporation, Bedford, MA). The lowest level of detection of the assay was 10 to 100 CFU. A total of 326 food samples were tested, both by culture and by PCR-FD. The overall sensitivity of the PCR-FD assay was 95.2% and the specificity was 89.9%. Positive and negative predictive values were 74.8% and 99.4%, respectively. Based on the results obtained in this study it appears that the PCR-FD assay described here can be useful...
The Polymerase Chain Reaction, 1994
Biopreservation and Biobanking, 2013
ABSTRACT According to their respective charters, both the European, Middle Eastern, and African S... more ABSTRACT According to their respective charters, both the European, Middle Eastern, and African Society for Biopreservation & Biobanking (ESBB) and the International Society for Biological and Environmental Repositories (ISBER) focus a sizeable share of their activities on repositories involving nonhuman material. Those specific collections may unfortunately not usually attract the same level of funding, visibility, and notoriety in the biobanking realm as their human counterparts. Nevertheless, biodiversity is a vast, largely unexploited resource of direct, indirect, or potential use for human health (www.cbd.int/doc/health/cohab-policy-brief1-en.pdf). Thus, biodiversity has recently become an important topic, as clues about understanding and elucidating health conditions affecting humans are increasingly being sought and encountered through the use of nonhuman models, be it animal, vegetal, or microbial. Also, indirect health issues and bio-economic aspects, as well as direct environmental and societal benefits in biodiversity biobanking, are often unfortunately ignored. Additionally, half of the most-prescribed drugs in the US, and an even higher fraction in developing countries, are derived from natural compounds—and many more are to be found. In biodiversity research, biobanks are especially relevant, because sample resources are frequently either very difficult to access or cannot be accessed repeatedly. The first situation arises from the remoteness of many collecting localities and the highly specialized knowledge necessary for collecting and identifying a plethora of species; the second can be due to human-induced rapid loss of populations or species. Studies about species evolution and adaptation to changing environmental conditions are some other aspects of the invaluable work done on samples from nonhuman repositories. Through skillful collection and conservation, it is becoming possible to accumulate snapshots of the status of specific species, arching back millions of years in some cases, and to assemble a ‘‘living archive’’ about how progressive evolution has enabled living organisms to adjust to incessantly changing circumstances. In this meeting report, we will summarize some of the presentations dealing with biodiversity, which were made at the recent ESBB meeting, held in Granada (Spain) in November 2012.
Journal of Infectious Diseases, 1988
Journal of Tissue Science and Engineering, Jul 31, 2015
Archives internationales de physiologie et de biochimie, 1977
Doctorat en Sciencesinfo:eu-repo/semantics/nonPublishe
筑波大学倫理学原論研究会, Dec 20, 2001
한반도 남동부에서 어일분지내의 마이오세 화산암류는 성분상 규장질(67.2-70.5wt.% SiO₂)과 염기성 (49.3-55.2wt.% SiO₂)으로 구성되는 쌍모식 조성을 ... more 한반도 남동부에서 어일분지내의 마이오세 화산암류는 성분상 규장질(67.2-70.5wt.% SiO₂)과 염기성 (49.3-55.2wt.% SiO₂)으로 구성되는 쌍모식 조성을 나타낸다. 이 분지에서의 쌍모식 화산작용은 분지 발달과 정과 밀접하게 연관되어 있으며, 화산암류는 분지내의 두터운 퇴적암층내에 협재되어 있으며, 특징적으로 반정 광물조합의 비평형을 나타낸다. 현무암질 용암에서는 융식된 석영 반정과 공존하는 감람석과 사방휘석 반정이 산출되고, 데사이트~유문암질 용결회류응회암에서는 사방휘석과 단사휘석이 반정으로 출현한다. 구성 광물상의 불균형으로부터 어일분지에서 일어난 쌍모식 화산활동에서 서로 다른 성분의 마그마 혼합이 크게 작용하였음을 시사해준다.
MGG Molecular & General Genetics, 1977
A new photoactivable reagent is described, which allows the formation of RNA-protein cross-links ... more A new photoactivable reagent is described, which allows the formation of RNA-protein cross-links via disulfide bridges in combination with mercaptobutyrimidate. The reconstituted L24 protein-23S RNA complex from the large subunit of E. coli ribosomes has been used as a model system for the cross-linking. The main advantages of the reagent are the absence of U.V. generated cross-links, since photoactivation is carried out at 360 nm, on one hand and the ease of cleavage of the cross-link by mild reduction (beta-mercaptoethanol) on the other.
Journal of Food Protection, 1995
This study evaluates a polymerase chain-reaction assay coupled with fluorescence detection (PCR-F... more This study evaluates a polymerase chain-reaction assay coupled with fluorescence detection (PCR-FD) in microwell plates for Listeria monocytogenes in dairy and food samples. Guanidinium thiocyanate/silica–extracted cultures and milk and dairy-food samples were used as templates for a PCR assay in microwell plates, with a primer pair that amplifies a 221 bp segment of the internal transcribed spacer (ITS), in the presence of 0.5 μg/ml of ethidium bromide. Fluorescence of the PCR products was measured with a CytoFluor 2300 fluorescence measurement system (Millipore Corporation, Bedford, MA). The lowest level of detection of the assay was 10 to 100 CFU. A total of 326 food samples were tested, both by culture and by PCR-FD. The overall sensitivity of the PCR-FD assay was 95.2% and the specificity was 89.9%. Positive and negative predictive values were 74.8% and 99.4%, respectively. Based on the results obtained in this study it appears that the PCR-FD assay described here can be useful...
The Polymerase Chain Reaction, 1994
Biopreservation and Biobanking, 2013
ABSTRACT According to their respective charters, both the European, Middle Eastern, and African S... more ABSTRACT According to their respective charters, both the European, Middle Eastern, and African Society for Biopreservation & Biobanking (ESBB) and the International Society for Biological and Environmental Repositories (ISBER) focus a sizeable share of their activities on repositories involving nonhuman material. Those specific collections may unfortunately not usually attract the same level of funding, visibility, and notoriety in the biobanking realm as their human counterparts. Nevertheless, biodiversity is a vast, largely unexploited resource of direct, indirect, or potential use for human health (www.cbd.int/doc/health/cohab-policy-brief1-en.pdf). Thus, biodiversity has recently become an important topic, as clues about understanding and elucidating health conditions affecting humans are increasingly being sought and encountered through the use of nonhuman models, be it animal, vegetal, or microbial. Also, indirect health issues and bio-economic aspects, as well as direct environmental and societal benefits in biodiversity biobanking, are often unfortunately ignored. Additionally, half of the most-prescribed drugs in the US, and an even higher fraction in developing countries, are derived from natural compounds—and many more are to be found. In biodiversity research, biobanks are especially relevant, because sample resources are frequently either very difficult to access or cannot be accessed repeatedly. The first situation arises from the remoteness of many collecting localities and the highly specialized knowledge necessary for collecting and identifying a plethora of species; the second can be due to human-induced rapid loss of populations or species. Studies about species evolution and adaptation to changing environmental conditions are some other aspects of the invaluable work done on samples from nonhuman repositories. Through skillful collection and conservation, it is becoming possible to accumulate snapshots of the status of specific species, arching back millions of years in some cases, and to assemble a ‘‘living archive’’ about how progressive evolution has enabled living organisms to adjust to incessantly changing circumstances. In this meeting report, we will summarize some of the presentations dealing with biodiversity, which were made at the recent ESBB meeting, held in Granada (Spain) in November 2012.
Journal of Infectious Diseases, 1988