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Papers by Ove Lillelund
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2016
Alpha-synuclein and familial variants affect the chain order and the thermotropic phase behavior ... more Alpha-synuclein and familial variants affect the chain order and the thermotropic phase behavior of anionic lipid vesicles
Proceedings of the National Academy of Sciences of the United States of America, Jan 8, 2015
Despite the ubiquity of helical membrane proteins in nature and their pharmacological importance,... more Despite the ubiquity of helical membrane proteins in nature and their pharmacological importance, the mechanisms guiding their folding remain unclear. We performed kinetic folding and unfolding experiments on 69 mutants (engineered every 2-3 residues throughout the 178-residue transmembrane domain) of GlpG, a membrane-embedded rhomboid protease from Escherichia coli. The only clustering of significantly positive ϕ-values occurs at the cytosolic termini of transmembrane helices 1 and 2, which we identify as a compact nucleus. The three loops flanking these helices show a preponderance of negative ϕ-values, which are sometimes taken to be indicative of nonnative interactions in the transition state. Mutations in transmembrane helices 3-6 yielded predominantly ϕ-values near zero, indicating that this part of the protein has denatured-state-level structure in the transition state. We propose that loops 1-3 undergo conformational rearrangements to position the folding nucleus correctly, ...
Acta Crystallographica Section B Structural Crystallography and Crystal Chemistry, 1978
... trans-1,2-Bis(methylthio)- 1,2-bis(phenylthio)ethylene BY RITA GRONBh~K HAZELL AND OVE K. LIL... more ... trans-1,2-Bis(methylthio)- 1,2-bis(phenylthio)ethylene BY RITA GRONBh~K HAZELL AND OVE K. LILLELUND Department of Inorganic Chemistry, A ... It was not possible to chaL~acterize them by spectroscopic means, so an X-ray investigation of the crystals was initiated and ...
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2016
Alpha-synuclein and familial variants affect the chain order and the thermotropic phase behavior ... more Alpha-synuclein and familial variants affect the chain order and the thermotropic phase behavior of anionic lipid vesicles
Proceedings of the National Academy of Sciences of the United States of America, Jan 8, 2015
Despite the ubiquity of helical membrane proteins in nature and their pharmacological importance,... more Despite the ubiquity of helical membrane proteins in nature and their pharmacological importance, the mechanisms guiding their folding remain unclear. We performed kinetic folding and unfolding experiments on 69 mutants (engineered every 2-3 residues throughout the 178-residue transmembrane domain) of GlpG, a membrane-embedded rhomboid protease from Escherichia coli. The only clustering of significantly positive ϕ-values occurs at the cytosolic termini of transmembrane helices 1 and 2, which we identify as a compact nucleus. The three loops flanking these helices show a preponderance of negative ϕ-values, which are sometimes taken to be indicative of nonnative interactions in the transition state. Mutations in transmembrane helices 3-6 yielded predominantly ϕ-values near zero, indicating that this part of the protein has denatured-state-level structure in the transition state. We propose that loops 1-3 undergo conformational rearrangements to position the folding nucleus correctly, ...
Acta Crystallographica Section B Structural Crystallography and Crystal Chemistry, 1978
... trans-1,2-Bis(methylthio)- 1,2-bis(phenylthio)ethylene BY RITA GRONBh~K HAZELL AND OVE K. LIL... more ... trans-1,2-Bis(methylthio)- 1,2-bis(phenylthio)ethylene BY RITA GRONBh~K HAZELL AND OVE K. LILLELUND Department of Inorganic Chemistry, A ... It was not possible to chaL~acterize them by spectroscopic means, so an X-ray investigation of the crystals was initiated and ...