Özgür Çelebi - Academia.edu (original) (raw)

Papers by Özgür Çelebi

FEMS Microbiology Letters, 2015

Türk Hijyen ve Deneysel Biyoloji Dergisi, 2012

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2006

Eurasian Journal of Veterinary Sciences, 2019

Journal of Advances in VetBio Science and Techniques, 2021

In this study, we aimed to determine the presence of pulmonary aspergillosis by histopathological... more In this study, we aimed to determine the presence of pulmonary aspergillosis by histopathological and microbiological methods in geese that are economically grown in the Kars region of Turkey. Totally 150 lung tissue samples of geese, average age of 9 weeks, which died between 2013 and 2020 and were brought to our department were included in the study. In order to reveal the presence of Aspergillus fungi, Periodic acid-Schiff (PAS) staining was applied to the sections as suggested by manufacturer. The microbiological examination of the tissue samples was carried out by the standard mycological culture technique on Sabouraud Dextrose Agar and by the phenotypical characterization of the emerged cultures. We observed large and small multifocal yellowish-white nodular structures in the lungs and air sacs macroscopically. In the histopathological examination of the lung tissues, we detected granulomatous structures with varying numbers and sizes. We diagnosed the Aspergillus agents in 20 (13,33%) of the tissue samples by detecting structures resembling typical tree branches in the middle of granulomatous structures with PAS staining. An identical positivity was obtained by the microbiological method and the emerged agent was solely identified as Aspergillus fumigatus with the growth pattern and macroscopic and microscopic morphological features. In conclusion, we found the presence of aspergillosis as 13,33% by histopathological and microbiological methods in geese which were brought to our department between 2013-2020. Based on these data, we concluded that aspergillosis is one of the most important infectious diseases among the goose deaths in the Kars region of Turkey

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2018

In this investigation, presence of Mannheimia haemolytica and Pasteurella multocida in sheep brou... more In this investigation, presence of Mannheimia haemolytica and Pasteurella multocida in sheep brought to Kafkas University Faculty of Veterinary Medicine between 2011 and 2013 with a suspicion of pneumonia was studied by immunohistochemical and bacteriological methods. Study materials were 100 sheep lungs. After routine histopathological investigations lungs were immunohistochemically stained with antibodies prepared against both of the agents. In bacteriological investigations, tissue samples were inoculated into 7% sheep blood agar and Mc Conkey agar and incubated for 24-48 h in aerobic conditions, and the suspected colonies were evaluated for Mannheimia and Pasteurella species. In histological investigations, pneumonias were classified as 28% fibrinous bronchopneumonia, 21% prulent bronchopneumonia, 20% acute-catharal bronchopneumonia, 18% interstitial pneumonia, 11% verminous pneumonia and 2% adenomatous pneumonia. In bacteriological investigations Mannheimia haemolytica and Pasteurella multocida was detected in 19 and 3 cases, respectively, these numbers were 17 and 2, respectively in immunohistochemical investigations. The results of PCR analysis for both Mannheimia haemolytica and Pasteurella multocida paralleled with the findings of microbiological culture. The results of the study showed that Mannheimia haemolytica is an important cause of pneumonia in sheep in Kars, and Pasteurella multocida, although with lesser importance can cause pneumonia in this species.

Advances in Animal and Veterinary Sciences, 2020

Listeria genus is Gram-positive facultative intracellular bacteria that are found in the soil as ... more Listeria genus is Gram-positive facultative intracellular bacteria that are found in the soil as saprophytes but also contain species that have pathogenic effects in their last host. Listeria monocytogenes is the major pathogen in Listeria species that is responsible for the majority of Listeriosis cases in humans and animals. In this study, it was aimed to investigate L. monocytogenes with histopathological and immunofluorescence (IF) methods in brain and liver tissues taken from sheep and cattle with clinical signs of suspicious Listeriosis. In the study, isolation and identification of L. monocytogenes with cultural methods were also applied. The material of the study consisted of tissue samples from 16 sheep and 2 cattle. As a result of cultural analysis, L. monocytogenes was identified from 12 (66.6%) of cases. Within the framework of histopathological and macroscopic findings, 16 (88.8%) of 18 cases with suspected Listeriosis were positive with the immunofluorescence method. T...

Eurasian Journal of Veterinary Sciences, 2013

Aim: This study was conducted for the purpose of identifying the microbiological, physico-chemic... more Aim: This study was conducted for the purpose of identifying the microbiological, physico-chemical and histological aspects of quality criteria in sausage-like products obtained from local markets and different samples of fermented sausages produced using traditional methods by local butcher shops in the province of Kars. Materials and Methods: Sampling was made during just one day and all the fermented sausages (n:30) and cooked sausage-like products (n:10) were purchased from all butchers and market points in Kars city centre. Microbiological analysis was performed by spread and/or pour plate techniques. Histological analysis was performed by Crossman triple stain and hematoxylin-eosin stains. Physicochemical analysis was performed by gravimetric and spectrophotometric methods. Results: Escherichia coli was identified in one of the cooked sausagelike products and in one sample of fermented sausage. Neither Clostridium perfringens nor E. coli O157:H7 were identified in any of th...

SUMMARY Brucellosis is an enzootic and economically important zoonotic infection in Turkey and th... more SUMMARY Brucellosis is an enzootic and economically important zoonotic infection in Turkey and the causative agent of ovine brucellosis in this region has not been genetically characterized in detail. This study aimed to isolate and identify Brucella melitensis from aborted sheep foetuses in Kars, Turkey and to characterize one of the B. melitensis isolate by 16S rRNA gene sequencing and Polymerase Chain Reaction (PCR)-Restriction Fragment Length Polymorphism (RFLP) of omp2a gene. B. melitensis was isolated and identified from 14 out of 37 aborted sheep foetuses and the 16S rRNA gene sequence of one of the B. melitensis isolate was identical to that of type strain of B. melitensis (16M strain). PCR-RFLP analyses of omp2a revealed that the current isolate was genetically close to the isolates from different Mediterranean countries, particularly those from France, Spain and Israel, however, different from vaccine (Rev.1) and type (16M) strains. The genetic characterization of B. melit...

A total of 167 blood samples from aborting sheep were collected from nine different villages and ... more A total of 167 blood samples from aborting sheep were collected from nine different villages and the serum samples were tested for the presence of Brucella melitensis antibodies by a serum agglutination test and Chlamydophila abortus antibodies by ELISA. Seventy-one (40.11%) and nine (5.38%) serum samples were found to be positive for B. melitensis and C. abortus, respectively. These results may indicate that both pathogens are the major infectious agents responsible for abortions in sheep in the Kars province.

Ankara Universitesi Veteriner Fakultesi Dergisi, 2016

Bu calismanin amaci brusella ile enfekte sigirlarda, bazi akut faz protein duzeylerinin belirlenm... more Bu calismanin amaci brusella ile enfekte sigirlarda, bazi akut faz protein duzeylerinin belirlenmesi ve diagnostik onemlerinin arastirilmasidir. Bu amacla calismada 20 adet brusella ile enfekte ve 10 adet saglikli hayvan kullanildi. Hayvanlarin V. jugularis’inden antikoagulansiz tuplere kan ornekleri toplandi. Kan ornekleri -20 oC de saklandi. Hazirlanan serum ornekleri haptoglobin (Hp), albumin ve seruloplazmin olcumlerinde kullanildi. Yapilan analiz sonucunda brusella ile enfekte sigirlar ile kontrol grubundaki hayvanlar karsilastirildiginda Hp ve seruloplazmin degerlerinin kontrol grubuna gore konsantrasyonunun yukseldigi, albumin degerinin ise kontrol grubuna gore konsantrasyonunun dustugu belirlendi. Sonuc olarak; yapilan bu calismada brusella ile enfekte hayvanlarda akut faz yanitin olustugu ve bunun sonucu olarak Hp ve seruloplazmin sentezinin arttigi, albumin sentezinin ise dustugu belirlenmistir

TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES, 2020

In order for a pathogen to successfully cause a disease, it must spread between different hosts. ... more In order for a pathogen to successfully cause a disease, it must spread between different hosts. In the case of Bacillus anthracis, disease progression is partly controlled by the presence of 2 virulence plasmids, while long-term survival is ensured through the ability to form spores. While the spore form of the organism is more resistant to chemical biocides and conventional antibiotics and unable to replicate, the more susceptible vegetative form can actively grow in a variety of media including milk [1,2]. Milk is an externally secreted fluid designed specifically to nourish the young. Microorganisms are rarely shed into both human and animal milk and this can be an important source of infections such as HIV, cytomegalovirus and human T-cell lymphotropic virus 1 (HTLV-1) of human [3] and Brucella spp., Campylobacter spp., and Mycobacterium bovis in animals [4,5]. However, B. anthracis-contaminated milk is rarely encountered and infrequently reported [6-8]. However, in general it is difficult to isolate the bacteria from milk once clinical symptoms appear due to high fever and reduction or cessation of milk production [9,10]. This case report investigates the history of a dairy cow that involuntarily secreted an unusual B. anthracis cell via its milk and presented it around the udder. 2. Case history A 6-year-old Simmental-Brown Swiss cross-bred cow was reported to have aborted in her first-trimester period and 10 days before microbiological sampling. Unfortunately, the aborted fetus and associated materials were destroyed before reporting. The cow was bred by the same owner since birth in a single barn with the remaining 20 cattle. The cow was farmed extensively on open pasture as much as possible during the autumn season and was barn-fed on hay and straw for the remainder of the year. A spore-based vaccine of Bacillus anthracis 34 F2 strain (Vetal, Turkey) was administered to all the cattle in the herd 6 months ago (October 2015). It was initially claimed by the farmer that the abortion was a result of common infectious agents and that milk contamination was coincidental. The cow was resampled after 3 days on the unexpected result of the first sampling. During sampling, the cow had normal physical findings (body score 2, body temperature 38 °C, normal pulse rate and appetite, no change in milk, and no signs of inflammation in the udder). The first batch sample was a milk cocktail taken from all the teats by the farmer. The second batch samples were obtained by the expert staff from the Veterinary Faculty at Kafkas University (Kars, Turkey). Swab samples were obtained

Journal of the Hellenic Veterinary Medical Society, 2018

Tuberculosis is an infectious bacterial disease of Bovidae and the causative agent is Mycobacteri... more Tuberculosis is an infectious bacterial disease of Bovidae and the causative agent is Mycobacterium bovis. It is responsible for remarkable economic losses among cattle herds with widely dispersion. Prompt and consistent diagnosis of tuberculosis especially in countries where the disease is endemic as in Turkey is of great importance to detect and identify infectious cases for strengthening control measures. In the present study, it was aimed to detect true animal and herd prevalence (within-herd, and between-herd) of antibodies against M. bovis in cattle herds. A serologic survey for antibody detection against the M. bovis was conducted by using an ELISA kit. Thirty three cattle herds were randomly selected from different farms and totally 460 cattle over five years of age were sampled. The true animal, within-herd, and between-herd prevalences found were 5.9% (95% CI = 3.0 to 8.8), 11.1% (95 CI = 6.5 to 15.8) and 73.4% (95 CI = 51.2 to 95.6), respectively. Results will provide use...

Veterinární Medicína, 2014

Paratuberculosis, caused by Mycobacterium avium subsp. paratuberculosis (Map), is one of the most... more Paratuberculosis, caused by Mycobacterium avium subsp. paratuberculosis (Map), is one of the most prevalent and costly infectious diseases of livestock, particularly sheep and cattle herds. The aim of this study was to estimate true animal, within-herd, and between-herd prevalence of Map antibodies in sheep herds of the Kars Region in the Northeast part of Turkey. A seroprevalence study was carried out using a commercial ELISA kit. Twenty six sheep herds, non-vaccinated against Map, were randomly selected in different regions and in total 450 sheep aged 24 months and more were sampled. Herds were declared positive if one or more sheep in the herd tested positive for Map antibodies. The animal, within-herd, and between-herd apparent prevalences were calculated as 6.2% (95% CI = 4.3 to 8.8%), 10.2% (95 CI = 7.1 to 14.3%) and 57.7% (95% CI = 38.9 to 74.5%), respectively. True prevalences were estimated by conversion from apparent prevalences via the Rogan-Gladen estimator. True animal,...

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Veterinary world, 2017

This study was conducted to determine the role ofin the formation of subclinical mastitis in cows... more This study was conducted to determine the role ofin the formation of subclinical mastitis in cows and to isolate the phage against isolatedstrains. In this study, 400 milk cows were screened by California Mastitis Test (CMT) for subclinical mastitis and 235 udders of 96 cows, which were determined to be positive, were evaluated for. Milk samples were evaluated using conventional and molecular methods. In addition, phage isolation studies were performed againststrains causing mastitis. At the result of cultural examination, of 235 milk samples that were found as positive for mastitis by CMT, a total of 117 (49.7%)spp. were isolated as a distribution of 74 (63.24%) coagulase-positive staphylococci and 43 (36.75%) coagulase-negative staphylococci. Of these isolates, 76 (64.95%) were characterized asboth conventional and molecular techniques. Lytic bacteriophages against twostrains which were isolated from mastitic milk samples were obtained from wastewater samples. The results of this ...

Eurasian Journal of Veterinary Sciences, 2017

Eurasian Journal of Veterinary Sciences Öz Amaç: Bu çalışmada Kafkas Üniversitesine ait Brucella ... more Eurasian Journal of Veterinary Sciences Öz Amaç: Bu çalışmada Kafkas Üniversitesine ait Brucella suş koleksiyonunun bir bölümü çeşitli yöntemler ile değerlendirilmiştir. Gereç ve Yöntem: Suşların orjini abort hikayesi olan sığır ve koyunlar oluşturudu. Otuz adet Brucella suşu (16'sı koyun ve 14'ü sığır orjinli) rastgele seçildi ve bu çalışmaya dahil edildi. Standardize fenotipik karakterizasyona ilaveten suşların moleküler tiplendirmesi için cins spesifik Real Time PCR ve tür spesifik Bruce-Ladder PCR uygulandı. Bulgular: Onaltı koyun örneğinin 15'i B. melitensis ve 14 sığır örneğinin 13'ü B. abortus olarak identifiye edildi. İlginçtir ki, koyun aborte fötusten izole edilen bir suş B. abortus ve sığırdan izole edilen bir suş B. melitensis olarak tanımlandı. Bununla birlikte, koyun fötusundan elde edilen bir suş bütün fenotipik özellikleri yönünden Brucella cinsine benzer karakterde olmasına rağmen genotipik olarak (ne Real Time PCR ne de Bruce-Ladder PCR) Brucella spp. olarak doğrulanamadı ve ileride karakterize edilmek üzere Ochrobactrum spp. şüpheli olarak saklandı. Öneri: Bu çalışma belli konaklar arasında çapraz Brucella infeksiyonunun sıradışı olarak nitelendirilebileceğini fakat zamanla bunun olağan olabileceğini göstermektedir. Bu şekildeki çalışmalar, Brucella cinsinin patojenitesini, konak spesifitesini ve gelişimini daha iyi anlamaya yardımcı olacaktır.

Indian Journal of Animal Research, 2017

Bovine herpesvirus-1 (BoHV-1) is well recognized as a pathogen that infects the respiratory and r... more Bovine herpesvirus-1 (BoHV-1) is well recognized as a pathogen that infects the respiratory and reproductive tracts. This study is a serological investigation of BoHV-1 in breeding bulls at 1–5 years of age from small scale family operations in three Northeastern Anatolian provinces (Kars, Ardahan and Igdir) in Turkey. For this purpose, blood was collected from 250 breeding bulls and was tested for antibodies against BoHV-1 using the virus neutralization technique. Out of 250 blood sera samples tested, 110 (44.00%) were detected as positive against to BoHV-1 and antibody titres were found to be varied between 1/2- 1/64. Among the controlled regions, the highest seroprevalance of BoHV-1 infection was found in Kars (64.81%) followed by Ardahan (30.00%) and Igdir (26.82%) provinces. The results suggested that the infection was spreading in breeding bulls in small scale family operations. This study is the first serological study to determine seroprevalence of BoHV-1 infection in breedi...

BMC veterinary research, Jan 12, 2017

Bacillus (B.) anthracis, the causal agent of anthrax, is effectively controlled by the Sterne liv... more Bacillus (B.) anthracis, the causal agent of anthrax, is effectively controlled by the Sterne live spore vaccine (34F2) in animals. However, live spore vaccines are not suitable for simultaneous vaccination and antibiotic treatment of animals being at risk of infection in an outbreak situation. Non-living vaccines could close this gap. In this study a combination of recombinant protective antigen and recombinant Bacillus collagen-like antigen (rBclA) with or without formalin inactivated spores (FIS), targeted at raising an immune response against both the toxins and the spore of B. anthracis, was tested for immunogenicity and protectiveness in goats. Two groups of goats received from local farmers of the Kars region of Turkey were immunized thrice in three weeks intervals and challenged together with non-vaccinated controls with virulent B. anthracis, four weeks after last immunization. In spite of low or none measurable toxin neutralizing antibodies and a surprisingly low immune re...