PABLO MARCO - Academia.edu (original) (raw)

Papers by PABLO MARCO

El texto describe y desarrolla los aspectos que se deben considerar al plantear el plan de trabaj... more El texto describe y desarrolla los aspectos que se deben considerar al plantear el plan de trabajo de una tesis de producción editorial de naturaleza gráfica.Facultad de Periodismo y Comunicación Socia

IV Jornadas de Investigación en Edición, Cultura y Comunicación (JIECC) (Buenos Aires, 2016), 2016

Frontiers in Microbiology, 2021

Monitored natural recovery (MNR) is an in situ technique of conventional remediation for the trea... more Monitored natural recovery (MNR) is an in situ technique of conventional remediation for the treatment of contaminated sediments that relies on natural processes to reduce the bioavailability or toxicity of contaminants. Metabarcoding and bioinformatics approaches to infer functional prediction were applied in bottom sediments of a tributary drainage channel of Río de La Plata estuary, in order to assess the biological contribution to MNR. Hydrocarbon concentration in water samples and surface sediments was below the detection limit. Surface sediments were represented with high available phosphorous, alkaline pH, and the bacterial classes Anaerolineae, Planctomycetia, and Deltaproteobacteria. The functional prediction in surface sediments showed an increase of metabolic activity, carbon fixation, methanogenesis, and synergistic relationships between Archaeas, Syntrophobacterales, and Desulfobacterales. The prediction in non-surface sediments suggested the capacity to respond to diff...

In this article we describe and explain the process of visual and editorial redesign carried out ... more In this article we describe and explain the process of visual and editorial redesign carried out by the professional team from the Scientific Publications Department of the Journalism and Social Communication Faculty of the National University of La Plata in the websites of electronic scientific magazines that the Faculty publishes through the server and editorial administrator Open Journal Systems (OJS). To achieve this, we analyze the marks, heads, backgrounds, institutional places and covers of the publications that are currently online in the Portal of Magazines of Journalism and Communication .

El material se encuentra disponible en el Portal de Revistas de Periodismo y Comunicacion de la F... more El material se encuentra disponible en el Portal de Revistas de Periodismo y Comunicacion de la Facultad de Periodismo y Comunicacion Social (ver "Enlace externo").

Documento elaborado por el equipo de la Direccion de Publicaciones Cientificas de la Facultad de ... more Documento elaborado por el equipo de la Direccion de Publicaciones Cientificas de la Facultad de Periodismo y Comunicacion Social (UNLP) en el marco del Programa de Capacitacion y Actualizacion Editorial.

En el trabajo se describe y se fundamenta el proceso de rediseño visual y editorial llevado a cab... more En el trabajo se describe y se fundamenta el proceso de rediseño visual y editorial llevado a cabo por el

Todo proceso de producción editorial supone la concreción de una serie de pasos que deben ser rea... more Todo proceso de producción editorial supone la concreción de una serie de pasos que deben ser realizados a fin de alcanzar el producto comunicacional que, según el caso, se ha decidido elaborar (libro, manual, revista, periódico, suplemento, etcétera). El cumplimiento de estas etapas, y la realización de las actividades que implica cada una de ellas, es lo que permitirá satisfacer la necesidad comunicacional detectada o contribuir a la solución de un problema comunicacional específico, mediante la producción del material editorial que resulte más conveniente. En el Plan de Tesis, la planificación de este proceso productivo debe estar reflejada en la formulación del objetivo general y de los objetivos específicos.

Los analogos de alquilfosfolipidos son un grupo de farmacos antitumorales que dirigidos hacia mem... more Los analogos de alquilfosfolipidos son un grupo de farmacos antitumorales que dirigidos hacia membrana, en lugar de danar al ADN, ejerciendo una variedad de acciones biologicas. En particular, estos agentes son candidatos prometedores en el tratamiento del cancer, puesto que presentan citotoxicidad selectiva contra varios tipos de tumores, inhibiendo el crecimiento de celulas transformadas. A pesar de que los alquilfosfolipidos se utilizan actualmente en ensayos clinicos contra el cancer, aun existe una gran falta de conocimiento acerca de su modo de accion. En esta tesis se ha trabajado con dos lineas de celulas tumorales humanas diferentes: HepG2, de hepatoblastoma y U-87 MG, de glioblastoma. En estas celulas la proliferacion celular y la progresion del ciclo celular han sido estudiadas mediante citometria de flujo y ensayos de colorimetria. Todos los alquilfosfolipidos utilizados en este estudio han mostrado inhibir la proliferacion celular y causar detencion del ciclo celular. C...

Journal of Human Kinetics, 2020

The use of flywheel devices has increased in popularity within resistance training programs. Howe... more The use of flywheel devices has increased in popularity within resistance training programs. However, little is known about modifiable variables which may affect power output responses, as the rope length and the height level used in a conical pulley device. The aim of this study was to assess the influence of using three different rope lengths (1.5, 2.5 and 3.5 meters) and four different height levels (L1, L2, L3 and L4) on concentric peak power (PPconc), eccentric peak power (PPecc) and eccentric overload (eccentric/concentric PP ratio; EO) during conical pulley exercises (i.e. seated and stand-up row). A total of 29 recreationally trained subjects (25.3±7.1 years; 1.74±0.06 m; 72.5±8.3 kg) took part in the study. Testing sessions consisted of 1 set of 10 repetitions under each condition; experiment 1: seated row exercise using the three different rope lengths; experiment 2: stand-up row exercise using four different height levels of the conical pulley. Results from experiment 1 d...

Scientific Reports, 2019

A full understanding of the molecular mechanism of action of choline kinase α (ChoKα) inhibitors ... more A full understanding of the molecular mechanism of action of choline kinase α (ChoKα) inhibitors at the cell level is essential for developing therapeutic and preventive approaches for cancer. The aim of the present study was to evaluate the effects of the ChoKα inhibitors EB-3D and EB-3P on lipid metabolism in HepG2 cells. We used [methyl-14C]choline, [1,2-14C]acetic acid and [2-3H]glycerol as exogenous precursors of the corresponding phospholipids and neutral lipids. [Methyl-14C]choline was also used to determine choline uptake. Protein levels were determined by Western blot. Ultrastructural alterations were investigated by transmission electron microscopy. In this work, we demonstrate that EB-3D and EB-3P interfere with phosphatidylcholine biosynthesis via both CDP-choline pathway and choline uptake by the cell. Moreover, the synthesis of both diacylglycerols and triacylglycerols was affected by cell exposure to both inhibitors. These effects were accompanied by a substantial dec...

Bioorganic & medicinal chemistry letters, Jan 31, 2018

In this article we present a series of non-cytotoxic potent human choline kinase (CK) inhibitors ... more In this article we present a series of non-cytotoxic potent human choline kinase (CK) inhibitors that exhibit nanomolar antiplasmodial activity in vitro. The most active antiplasmodial compounds, 10a-b, bearing a pyridinium cationic head were inactive against CK, while compounds 10g and 10j with a quinolinium moiety exhibit moderate inhibition of both the parasite and the enzyme. The results point towards an additional mechanism of action unrelated to CK inhibition that remains to be established.

Frontiers in microbiology, 2017

The genus Flavivirus comprises a large number of small, positive-sense single-stranded, RNA virus... more The genus Flavivirus comprises a large number of small, positive-sense single-stranded, RNA viruses able to replicate in the cytoplasm of certain arthropod and/or vertebrate host cells. The genus, which has some 70 member species, includes a number of emerging and re-emerging pathogens responsible for outbreaks of human disease around the world, such as the West Nile, dengue, Zika, yellow fever, Japanese encephalitis, St. Louis encephalitis, and tick-borne encephalitis viruses. Like other RNA viruses, flaviviruses have a compact RNA genome that efficiently stores all the information required for the completion of the infectious cycle. The efficiency of this storage system is attributable to supracoding elements, i.e., discrete, structural units with essential functions. This information storage system overlaps and complements the protein coding sequence and is highly conserved across the genus. It therefore offers interesting potential targets for novel therapeutic strategies. This ...

New Scientist, 2016

Marco remarks on the bombing of rebel-held areas that's deepening a desperate health crisis i... more Marco remarks on the bombing of rebel-held areas that's deepening a desperate health crisis in Aleppo. Months of siege and the recent intensification of indiscriminate bombing by the Syrian government and its allies have left the population of eastern Aleppo in desperate straits and its health system in tatters. The doctors report an increase in child malnutrition and waterborne diseases. Some mothers are too weak to breastfeed, and a lack of formula milk and other basics is leading to preventable deaths. What is needed is an end to the indiscriminate airstrikes on civilian areas, respect for medical facilities and patients, and an end to the crippling siege.

Scientific Reports, 2016

The cis-acting replication element (CRE) of the hepatitis C virus (HCV) RNA genome is a region of... more The cis-acting replication element (CRE) of the hepatitis C virus (HCV) RNA genome is a region of conserved sequence and structure at the 3′ end of the open reading frame. It participates in a complex and dynamic RNA-RNA interaction network involving, among others, essential functional domains of the 3′ untranslated region and the internal ribosome entry site located at the 5′ terminus of the viral genome. A proper balance between all these contacts is critical for the control of viral replication and translation, and is likely dependent on host factors. Proteomic analyses identified a collection of proteins from a hepatoma cell line as CRE-interacting candidates. A large fraction of these were RNA-binding proteins sharing highly conserved RNA recognition motifs. The vast majority of these proteins were validated by bioinformatics tools that consider RNA-protein secondary structure. Further characterization of representative proteins indicated that hnRNPA1 and HMGB1 exerted negative...

The synthetic lipid analogue, hexadecylphosphocholine is an antitumoral and antileishmanial agent... more The synthetic lipid analogue, hexadecylphosphocholine is an antitumoral and antileishmanial agent that acts on cell membranes and can induce apoptosis. We have previously investigated the effect of hexadecylphosphocholine on the biosynthesis and intracellular transport of cholesterol in the human hepatoma HepG2 cell line. Here we show that the traffic of endocytosed-cholesterol from LDL to the plasma membrane and the transport of newly synthesized cholesterol from the endoplasmic reticulum to the plasma membrane were unaffected by alkylphosphocholine exposure. On the contrary, cholesterol traffic from the plasma membrane to the endoplasmic reticulum was drastically interrupted after 1 h of cell exposition to HePC and, consequently, the intracellular esterification of cholesterol was substantially decreased. Our results also demonstrate that this alkylphosphocholine exclusively affected the nonvesicular, energy-independent cholesterol traffic, without altering the vesicular transport. In addition, hydrolysis of plasma membrane sphingomyelin by exogenously added sphingomyelinase resulted in enhanced plasma-membrane cholesterol esterification, but sphingomyelinase treatment did not prevent the inhibition in cholesteryl ester formation caused by hexadecylphosphocholine. We also found that sphingomyelin synthesis was significantly inhibited in HepG2 cells after exposure to hexadecylphosphocholine. Since sphingomyelin and cholesterol are major lipid constituents of membrane raft microdomains, these results suggest that hexadecylphosphocholine could disturb membrane raft integrity and thence its functionality.

Proceedings of 1st International Electronic Conference on Medicinal Chemistry, 2015