PALASH MANDAL - Academia.edu (original) (raw)
Research article by PALASH MANDAL
Objective: To observe the influence of extraction processes on the bioactivity of Fraxinus florib... more Objective: To observe the influence of extraction processes on the bioactivity of Fraxinus floribunda (FF) bark on the basis
of antioxidant activity and assess the in vivo anti-inflammatory with hepatoprotective activity in the extracts which has
showed better antioxidant potential. Materials and Methods: Antioxidant activity on the basis of phytochemical estimation,
free radical scavenging, and antilipid peroxidation activity. Anti-inflammatory activity was observed through carrageenan-
induced paw edema, and hepatoprotective effect was studied using carbon tetrachloride induced liver damage in Wister
albino rats. Results: Autoclave boiled extract showed highest antioxidant activity in assays of total phenol content (26.98 ±
11.22 mg gallic acid equivalent/g fresh weight tissue), 2,2-diphenyl-1-picryl hydrazyl (0.24 ± 3.65 mg/ml), etc. The same
extract inhibited mice paw edema by 55% which was significant after 2 h (P < 0.05) showing anti-inflammatory activity.
Lowering of serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, anti-lipid peroxidation, and
bilirubin levels showed hepatoprotective activity against standard formulation Liv-52. Conclusion: The bark of FF is a
potential source of antioxidants with anti-inflammatory and hepatoprotective activity. Further research should be done to
explore and validate its bioactivity
Papers by PALASH MANDAL
Climatic variation of Phenolics (mainly EGCG and ECG) Flavouring index (TF: TR and HPS, TC), PPO ... more Climatic variation of Phenolics (mainly EGCG and ECG) Flavouring index (TF: TR and HPS, TC), PPO activities and anti-oxidative properties in fresh tea shoots to handmade orthodox tea, consisting of one apical bud and two adjoining leaves sampled from TV-1, TV20, TV26, TV29, TV30 (Tocklai Vegetative) clone and Tingamara, Dangri Manipuri and Sundaram B/5/63(Seed jat), Tinali 17, Takda7,8 grown in Terai, Hill and Dooarsregion in North Bengal, were investigated during three harvest season (March, June and December) in 2007, 2008,2009 and 2010. The EGCG, ECG of all clones was lower in cool months of December in four years (average 4.90-2.15, 2.93-0.63). Thereafter, the levels of total Phenolics in terms of EGCG, ECG increased throughout the warmer months from March to September. Antioxidant activity showed similar trends which increased from 1st harvest (March) to 3rd harvest (December). All clones showed nearly 100% antioxidant activity at 2nd and 3rd harvest season which higher than st...
International Journal of Integrative …, 2009
In different parts of plain land and hill area of Darjeeling district, leaves and stems of Drymar... more In different parts of plain land and hill area of Darjeeling district, leaves and stems of Drymaria diandra were evaluated for their phytochemical constituents like total phenol, ortho-dihydric phenol, flavonols, tannins and antioxidant activity against 2,2-diphenyl-2-picrylhydrazyl ( ...
Scientific Reports
Morus alba is one of the most important cultivated crop in Indian sub-continent contributing towa... more Morus alba is one of the most important cultivated crop in Indian sub-continent contributing towards production of silk fibre that carries economic importance worldwide. Post harvest preservation of M. alba leaves is a challenging factor as decrease in concentration of essential metabolites that needed for silk gland development takes place. Decrease in chlorophyll, protein, sugar concentration and increase in accumulation of free radicals and ROS takes place at post harvest stage of preservation, putting negative impact on larval development indicated by high mortality rate. Silver nitrate and nanosilver solution acts as an effective preservative, enhances the activity of enzymatic and non-enzymatic antioxidants thereby reducing the harmful effect of accumulated free radicals and ROS. The effectiveness of nanosilver solution was found to be on the upper site without any significant difference than silver nitrate, as higher retention of primary metabolites like pigments, proteins, a...
Impact of Physical Attributes on Proficient Phytosynthesis of Silver Nanoparticles Using Extract of Fresh Mulberry Leaves: Characterization, Stability and Bioactivity Assessment
Journal of Inorganic and Organometallic Polymers and Materials
Application of green chemistry towards phytosynthesis of nanoparticles has gained attention of en... more Application of green chemistry towards phytosynthesis of nanoparticles has gained attention of environment friendly world due to its simple, rapid, stable, eco-friendly and cost-effective property. But there are limitations to this growing interest, as the dimension and geometry of nanoparticles produced through phytosynthesis often varies greatly. To overcome the problem, current study deals with the assessment of reducing effect of the plant extract at different light intensity, pH and temperatures. Current study reports that the optimal condition for phytosynthesis of silver nanoparticles was neutral pH (pH 7), 25 °C and diffuse light (230 lx). It was observed that rapid synthesis of silver nanoparticles takes place with increase in light intensity, alkalinity and temperature, as revealed by quick appearance of dark brown colouration on addition of extract. But rapid synthesis leads to the formation of large size particles with increase in polydispersity as revealed from TEM analysis and were found to be instable through zeta potential analysis. It was observed that at acidic pH nanosilver formation does not takes place, while at low temperature it takes more time for nanosilver formation, but both conditions were found to be unstable. Nanosilver formed at neutral pH and 25 °C under diffuse light gives the size dimension of 18.11 ± 2.55 and 18.94 ± 5.30 nm with zeta potential of + 36.4 and + 37.4 mV respectively indicating stability, besides that they were found to be stable under long storage condition as revealed by UV–Visible spectrophotometric analysis. Silver nanoparticles formed under optimized condition of pH 7 and at 25 °C were found to be bioactive as it showed potentiality to extend the shelf life of post harvest mulberry leaves by 7 days.
Pharmacognosy Journal
Introduction: Dysmenorrhea is a sort of painful menstrual disorder. Several plants like Allium sa... more Introduction: Dysmenorrhea is a sort of painful menstrual disorder. Several plants like Allium sativum L., Areca catechu L., Zingiber officinale Roscoe, Crinum amoenum Ker Gawl. Ex Roxb, Cuscuta reflexa Roxb. Nymphaea rubra Roxb. Ex Andrews, Piper nigrum L., Citrus limon (L.) Osbeck were used in appropriate ratio to make herbal formulation to cure dysmenorrheal pain by the traditional healers of Rajbanshi community. Objective: By semi-structured questionnaires in the course of scheduled interviews with the local herbal practitioners, four herbal formulations (coded as DYS1, DYS2, DYS3 and DYS4) were recorded. Pharmacognostic characterization was carried out for the authentication of the powder drugs which includes powder microscopy, fluorescence analysis and physicochemical evaluation. The presence of any therapeutic potential in DYS1, DYS2, DYS3 and DYS4 was determined by qualitative evaluation of phytochemicals of various successive solvent extracts based on their polarity. Thin layer chromatography was performed for screening various phyto-active compounds like arbutin, alkaloids, coumarins, cardiac glycoside, etc. Results: Powder microscopy revealed the presence of calcium oxalate crystal, stone cells etc. Various fluorescence colors were exhibited by these herbal drugs under UV after reacting with chemical reagents. Physical analyses values were also obtained in a satisfactory way. TLC and qualitative phytochemical analysis showed the presence of active phytoconstituents like arbutin, alkaloids, coumarins, cardiac glycoside etc. Conclusion: The results support the use of plants as a traditional medicine for the prevention disorders like Dysmenorrhea. Further evaluation is required for determining the efficacy of those herbal drugs and the active phytoconstituents involved in Dysmenorrheal therapy.
Journal of Central European Agriculture
The objective of present study was to evaluate the response of the mung bean seeds of 'Sonali B1'... more The objective of present study was to evaluate the response of the mung bean seeds of 'Sonali B1' variety primed with chitosan in four different concentrations (0, 0.1%, 0.2% and 0.5%) under salinity stress of five different concentrations (i.e., 0, 4, 6, 8 and 12 dS*mm-1) and halotolerance pattern by applying Celite as matrix at three different moisture levels (5%, 10% and 20%). Improved germination percentage, germination index, mean germination time, coefficient of velocity of germination along with root and shoot length was observed comparing with control. Germination stress tolerance index (GSI), plant height stress tolerance index (PHSI) and root length stress tolerance index (RLSI) were used to evaluate the tolerance of the mung bean seeds against salinity stress induced by chitosan. Results of GSI, PHSI, RLSI showing noteworthy inhibitory effect of salinity stress in control set was significantly less pronounced in chitosan treated seedlings. Chitosan can remarkably alleviate the detrimental effect of salinity up to the level of 6 dS*m-1 , beyond which no improvement was noticed. In conclusion present investigation revealed that chitosan is an ideal elicitor for enhancing the speed of germination and seedling invigoration that synchronize with emergence of radicle and salinity stress tolerance.
BMC Complementary and Alternative Medicine
Background: Study of phytochemicals and pharmacological properties of bryophytes has been neglect... more Background: Study of phytochemicals and pharmacological properties of bryophytes has been neglected for a long time because of the three main reasons i.e. (i) difficulty in collection in large amount for analysis; (ii) their availablility only in particular season and (iii) their restricted geographic distribution. So, the aim of this work was to propagate Lunularia cruciata under in vitro condition for comparing its pharmacological properties and phytocmecial constituents with naturally grown counterparts. Method: Axenic culture of L. cruciata was established by propagating gemmae under in vitro condition. Appropriate culture conditions, media, and the effect of hormones on growth and development were studied. The phytochemical composition was determined by GC-MS analysis and pharmacological activity was evaluated by assessing the antioxidant and anti-diabetic activities. For the antioxidant activity ABTS + [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)], DPPH • (2,2-diphenyl-1-picrylhydrazyl) and metal chelating assays were done and for evaluation of the in vitro anti-diabetic activity α-glucosidase and α-amylase inhibitory activities were done. Result: Growth of L. cruciata was recorded in half strength MS media. Benzylaminopurine (BAP: 2 mg/L) and 1-Naphtheleneacetic acid (NAA: 0.5 mg/L) were the successful hormonal combination. GC-MS analysis revealed the existence of nine key compounds in both in vitro and naturally grown L. cruciata. Result of antioxidant and anti-diabetic activity showed that in vitro grown L. cruciata has a more or less similar antioxidant and anti-diabetic activities as naturally grown ones. This result confirms the possibility of using in vitro grown plants in place of naturally grown plants for research and clinical purposes.
In vitro antioxidant and antidiabetic activity of oligopeptides derived from different mulberry (Morus alba L.) cultivars
Pharmacognosy Research
International Journal of Pharmacy and Pharmaceutical Sciences
Objective: To evaluate the effect of ultraviolet (UV) radiation on the therapeutical and oxidativ... more Objective: To evaluate the effect of ultraviolet (UV) radiation on the therapeutical and oxidative stress status of fenugreek seedlings during the post-germination phase.Methods: The seeds of Trigonella foenum-graecum L. were subjected to UV radiation of different wavelengths UV 254 nm, UV 365 nm and the combination of 254 nm and 365 nm UV during the course of germination up to 96 h. Free radicals scavenging activity, in vitro antidiabetic activity as well as total phenol (TPC) and flavonoid (TFC) contents were evaluated up to four days of germination stages. Analysis of total activities of superoxide dismutase (SOD), peroxidase, catalase and NADPH oxidase (NOX) was conducted using non-denaturing polyacrylamide gel electrophoresis along with histochemical detection of H2O2 and superoxide radical localisation, lipid peroxidation and plasma membrane integrity was also performed.Results: Overall it was observed that the sprouts exposed to UV 365 nm exhibited a significant enhancement i...
Asian Journal of Pharmaceutical and Clinical Research
Objective: This work was performed to evaluate the effect of priming with exogenous sources of ca... more Objective: This work was performed to evaluate the effect of priming with exogenous sources of calcium ion and nitric oxide on the antidiabeticactivity and the alkaloid contents of fenugreek sprouts along with isolation and identification of trigonelline, a bioactive alkaloid responsible for hypoglycemic property of fenugreek.Methods: The fenugreek seeds were pre-treated with calcium chloride (CC), lanthanum chloride (LC) a calcium channel blocker; ethylene glycol-bis (2-aminoethylether) -N, N, N´, N tetra acetic acid (EG) a calcium chelator; sodium nitroprusside (SNP) and 2-(4-carboxyphenyl) -4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (CP) a nitric oxide scavenger and germinated for 72 hrs. The sprout extracts were evaluated for their in vitro antidiabetic potential by α-amylase and α-glucosidase inhibition along with their trigonelline content. Trigonelline was isolated from fenugreek sprouts and identified by Infrared analysis and nuclear magnetic resonance (NMR) spectroscopy....
Tea Waste Management: A Case Study from West Bengal, India
Indian Journal of Science and Technology, 2016
Changes in Antioxidant Activity of Cyphomandra betacea (Cav.) Sendtn. Fruits During Maturation and Senescence
International Journal of Food Properties, 2011
ABSTRACT Antioxidants are extremely important substances which possess the ability to protect the... more ABSTRACT Antioxidants are extremely important substances which possess the ability to protect the body from damage caused by free radical induced oxidative stress. Antioxidants are derived from dietary sources like fruits, vegetables and beverages. In this study the antioxidant activity of different maturity stages of two varieties of Cyphomandra betacea fruits of Darjeeling were evaluated in vitro. The radical scavenging properties on 2,2 Diphenyl-1-picrylhydrazyl (DPPH), superoxide anion, hydroxyl radical, lipid peroxidation, nitric oxide and reducing power as well as the flavonoids, phenolics, lycopene and total carotene contents of methanolic extracts of the fruits were determined. All fruit extracts, mainly the mature red fruit of Purple-red variety exhibited strong scavenging activity towards all radicals tested due to the presence of relatively high total phenol, flavonoids, lycopene as well as total carotene contents. Our findings suggest that purple red variety of C. betacea fruit is endowed with antioxidant phytochemicals which could provide protection against oxidative stress induced diseases.
Phytochemical Screening and Antioxidant Activities of Two Selected ‘Bihi’ Fruits Used as Vegetables in Darjeeling Himalaya (Accepted)
In vitro detection of antioxidants in different solvent fractions of Ginger (Zingiber officinale Rosc.)
Indian Journal of Plant Physiology, 2009
... 23 Indian J. Plant Physiol., Vol. 14, No. 1, (NS) pp. 23-27 (Jan.-March, 2009) IN VITRO DETEC... more ... 23 Indian J. Plant Physiol., Vol. 14, No. 1, (NS) pp. 23-27 (Jan.-March, 2009) IN VITRO DETECTION OF ANTIOXIDANTS IN DIFFERENT SOLVENT FRACTIONS OF GINGER (ZINGIBER OFFICINALE ROSC.) MALAY BHATTACHARYA, PALASH MANDAL AND ARNAB SEN* ...
Antioxidant Activities of Different Parts of Tree Tomato Fruit (Accepted)
In Vitro Antioxidant Activity of Edible Timbur Fruits of Darjeeling Himalaya
In-Vitro free radical scavenging activities of the leaves of Malva verticillata L
Wheat (Triticum aestivum) peptide (s) mimic gibberellin action and regulate stomatal opening
Indian Journal of Experimental Biology, 2010
Wheat peptides (0.5 to 3 KDa M(r)) mimick hormonal activity like that of gibberellins and forced ... more Wheat peptides (0.5 to 3 KDa M(r)) mimick hormonal activity like that of gibberellins and forced open dark closed stomata. The deionized amphoteric peptides solution after passing through cation and anion exchanger resins was run through Amicon&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s ultrafilters, 10, 3 and 0.5 kDa (M(r)) cut off system. The 3 to 0.5 kDa fraction passed through sephadex LH-20 column and collected in 140 tubes (5 ml in each tube). The two fractions F 9 (91-100 tubes) and F 12 (121-130) were found much active on stomatal opening and a-amylase activity, respectively and were ninhydrin positive. Capillary electrophoresis of F 9 fraction yielded several peptides ranging 1600 to 2200 (M(r) and F 12 fraction showed 1800 - 2800(M(r)). Both the fractions were totally hydrolysed for amino acid analysis by HPLC. Most of the amino acids were present except cystein in both the fractions. The F 9 fraction, (peptide present in 10 microg fresh wt tissue per ml) induced the dark grown closed stomata to open upto 70%. In F 12 fraction, (peptide present in 10 microg fresh wt equivalent tissue per ml) showed alpha-amylase induction which was much higher than GA(3) (10(9) M). The peptide might be present in membrane and bound with GA that activated alpha-amylase m-RNA synthesis. The peptide might act directly on alpha-amylase gene.
![Research paper thumbnail of Diversity of Litsea Lamarck [Lauraceae] in Terai and Duars regions of West Bengal, India](https://attachments.academia-assets.com/68477808/thumbnails/1.jpg)
](There are about 622 species in the genus Litsea Lamarck (Lauraceae), distributed mainly in Austra... more There are about 622 species in the genus Litsea Lamarck (Lauraceae), distributed mainly in Australia, New Zealand, North & South America and Asia. Among those, 45 species of Litsea were reported from India. No comprehensive report is available on this genus from Terai and Duars regions, which are located at the foot of the Eastern Himalaya and located within the Himalaya Hotspot. Present paper reported the occurrence of nine species of Litsea from Terai and Duars belt along with their local names, salient features, exsiccatus, status, flowering and fruiting periods and geographic distribution. Collection of L. assamica Hooker f. from the Jaldapara National Park in Duars forms the new record for the state flora of West Bengal.
Objective: To observe the influence of extraction processes on the bioactivity of Fraxinus florib... more Objective: To observe the influence of extraction processes on the bioactivity of Fraxinus floribunda (FF) bark on the basis
of antioxidant activity and assess the in vivo anti-inflammatory with hepatoprotective activity in the extracts which has
showed better antioxidant potential. Materials and Methods: Antioxidant activity on the basis of phytochemical estimation,
free radical scavenging, and antilipid peroxidation activity. Anti-inflammatory activity was observed through carrageenan-
induced paw edema, and hepatoprotective effect was studied using carbon tetrachloride induced liver damage in Wister
albino rats. Results: Autoclave boiled extract showed highest antioxidant activity in assays of total phenol content (26.98 ±
11.22 mg gallic acid equivalent/g fresh weight tissue), 2,2-diphenyl-1-picryl hydrazyl (0.24 ± 3.65 mg/ml), etc. The same
extract inhibited mice paw edema by 55% which was significant after 2 h (P < 0.05) showing anti-inflammatory activity.
Lowering of serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, anti-lipid peroxidation, and
bilirubin levels showed hepatoprotective activity against standard formulation Liv-52. Conclusion: The bark of FF is a
potential source of antioxidants with anti-inflammatory and hepatoprotective activity. Further research should be done to
explore and validate its bioactivity
Climatic variation of Phenolics (mainly EGCG and ECG) Flavouring index (TF: TR and HPS, TC), PPO ... more Climatic variation of Phenolics (mainly EGCG and ECG) Flavouring index (TF: TR and HPS, TC), PPO activities and anti-oxidative properties in fresh tea shoots to handmade orthodox tea, consisting of one apical bud and two adjoining leaves sampled from TV-1, TV20, TV26, TV29, TV30 (Tocklai Vegetative) clone and Tingamara, Dangri Manipuri and Sundaram B/5/63(Seed jat), Tinali 17, Takda7,8 grown in Terai, Hill and Dooarsregion in North Bengal, were investigated during three harvest season (March, June and December) in 2007, 2008,2009 and 2010. The EGCG, ECG of all clones was lower in cool months of December in four years (average 4.90-2.15, 2.93-0.63). Thereafter, the levels of total Phenolics in terms of EGCG, ECG increased throughout the warmer months from March to September. Antioxidant activity showed similar trends which increased from 1st harvest (March) to 3rd harvest (December). All clones showed nearly 100% antioxidant activity at 2nd and 3rd harvest season which higher than st...
International Journal of Integrative …, 2009
In different parts of plain land and hill area of Darjeeling district, leaves and stems of Drymar... more In different parts of plain land and hill area of Darjeeling district, leaves and stems of Drymaria diandra were evaluated for their phytochemical constituents like total phenol, ortho-dihydric phenol, flavonols, tannins and antioxidant activity against 2,2-diphenyl-2-picrylhydrazyl ( ...
Scientific Reports
Morus alba is one of the most important cultivated crop in Indian sub-continent contributing towa... more Morus alba is one of the most important cultivated crop in Indian sub-continent contributing towards production of silk fibre that carries economic importance worldwide. Post harvest preservation of M. alba leaves is a challenging factor as decrease in concentration of essential metabolites that needed for silk gland development takes place. Decrease in chlorophyll, protein, sugar concentration and increase in accumulation of free radicals and ROS takes place at post harvest stage of preservation, putting negative impact on larval development indicated by high mortality rate. Silver nitrate and nanosilver solution acts as an effective preservative, enhances the activity of enzymatic and non-enzymatic antioxidants thereby reducing the harmful effect of accumulated free radicals and ROS. The effectiveness of nanosilver solution was found to be on the upper site without any significant difference than silver nitrate, as higher retention of primary metabolites like pigments, proteins, a...
Impact of Physical Attributes on Proficient Phytosynthesis of Silver Nanoparticles Using Extract of Fresh Mulberry Leaves: Characterization, Stability and Bioactivity Assessment
Journal of Inorganic and Organometallic Polymers and Materials
Application of green chemistry towards phytosynthesis of nanoparticles has gained attention of en... more Application of green chemistry towards phytosynthesis of nanoparticles has gained attention of environment friendly world due to its simple, rapid, stable, eco-friendly and cost-effective property. But there are limitations to this growing interest, as the dimension and geometry of nanoparticles produced through phytosynthesis often varies greatly. To overcome the problem, current study deals with the assessment of reducing effect of the plant extract at different light intensity, pH and temperatures. Current study reports that the optimal condition for phytosynthesis of silver nanoparticles was neutral pH (pH 7), 25 °C and diffuse light (230 lx). It was observed that rapid synthesis of silver nanoparticles takes place with increase in light intensity, alkalinity and temperature, as revealed by quick appearance of dark brown colouration on addition of extract. But rapid synthesis leads to the formation of large size particles with increase in polydispersity as revealed from TEM analysis and were found to be instable through zeta potential analysis. It was observed that at acidic pH nanosilver formation does not takes place, while at low temperature it takes more time for nanosilver formation, but both conditions were found to be unstable. Nanosilver formed at neutral pH and 25 °C under diffuse light gives the size dimension of 18.11 ± 2.55 and 18.94 ± 5.30 nm with zeta potential of + 36.4 and + 37.4 mV respectively indicating stability, besides that they were found to be stable under long storage condition as revealed by UV–Visible spectrophotometric analysis. Silver nanoparticles formed under optimized condition of pH 7 and at 25 °C were found to be bioactive as it showed potentiality to extend the shelf life of post harvest mulberry leaves by 7 days.
Pharmacognosy Journal
Introduction: Dysmenorrhea is a sort of painful menstrual disorder. Several plants like Allium sa... more Introduction: Dysmenorrhea is a sort of painful menstrual disorder. Several plants like Allium sativum L., Areca catechu L., Zingiber officinale Roscoe, Crinum amoenum Ker Gawl. Ex Roxb, Cuscuta reflexa Roxb. Nymphaea rubra Roxb. Ex Andrews, Piper nigrum L., Citrus limon (L.) Osbeck were used in appropriate ratio to make herbal formulation to cure dysmenorrheal pain by the traditional healers of Rajbanshi community. Objective: By semi-structured questionnaires in the course of scheduled interviews with the local herbal practitioners, four herbal formulations (coded as DYS1, DYS2, DYS3 and DYS4) were recorded. Pharmacognostic characterization was carried out for the authentication of the powder drugs which includes powder microscopy, fluorescence analysis and physicochemical evaluation. The presence of any therapeutic potential in DYS1, DYS2, DYS3 and DYS4 was determined by qualitative evaluation of phytochemicals of various successive solvent extracts based on their polarity. Thin layer chromatography was performed for screening various phyto-active compounds like arbutin, alkaloids, coumarins, cardiac glycoside, etc. Results: Powder microscopy revealed the presence of calcium oxalate crystal, stone cells etc. Various fluorescence colors were exhibited by these herbal drugs under UV after reacting with chemical reagents. Physical analyses values were also obtained in a satisfactory way. TLC and qualitative phytochemical analysis showed the presence of active phytoconstituents like arbutin, alkaloids, coumarins, cardiac glycoside etc. Conclusion: The results support the use of plants as a traditional medicine for the prevention disorders like Dysmenorrhea. Further evaluation is required for determining the efficacy of those herbal drugs and the active phytoconstituents involved in Dysmenorrheal therapy.
Journal of Central European Agriculture
The objective of present study was to evaluate the response of the mung bean seeds of 'Sonali B1'... more The objective of present study was to evaluate the response of the mung bean seeds of 'Sonali B1' variety primed with chitosan in four different concentrations (0, 0.1%, 0.2% and 0.5%) under salinity stress of five different concentrations (i.e., 0, 4, 6, 8 and 12 dS*mm-1) and halotolerance pattern by applying Celite as matrix at three different moisture levels (5%, 10% and 20%). Improved germination percentage, germination index, mean germination time, coefficient of velocity of germination along with root and shoot length was observed comparing with control. Germination stress tolerance index (GSI), plant height stress tolerance index (PHSI) and root length stress tolerance index (RLSI) were used to evaluate the tolerance of the mung bean seeds against salinity stress induced by chitosan. Results of GSI, PHSI, RLSI showing noteworthy inhibitory effect of salinity stress in control set was significantly less pronounced in chitosan treated seedlings. Chitosan can remarkably alleviate the detrimental effect of salinity up to the level of 6 dS*m-1 , beyond which no improvement was noticed. In conclusion present investigation revealed that chitosan is an ideal elicitor for enhancing the speed of germination and seedling invigoration that synchronize with emergence of radicle and salinity stress tolerance.
BMC Complementary and Alternative Medicine
Background: Study of phytochemicals and pharmacological properties of bryophytes has been neglect... more Background: Study of phytochemicals and pharmacological properties of bryophytes has been neglected for a long time because of the three main reasons i.e. (i) difficulty in collection in large amount for analysis; (ii) their availablility only in particular season and (iii) their restricted geographic distribution. So, the aim of this work was to propagate Lunularia cruciata under in vitro condition for comparing its pharmacological properties and phytocmecial constituents with naturally grown counterparts. Method: Axenic culture of L. cruciata was established by propagating gemmae under in vitro condition. Appropriate culture conditions, media, and the effect of hormones on growth and development were studied. The phytochemical composition was determined by GC-MS analysis and pharmacological activity was evaluated by assessing the antioxidant and anti-diabetic activities. For the antioxidant activity ABTS + [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)], DPPH • (2,2-diphenyl-1-picrylhydrazyl) and metal chelating assays were done and for evaluation of the in vitro anti-diabetic activity α-glucosidase and α-amylase inhibitory activities were done. Result: Growth of L. cruciata was recorded in half strength MS media. Benzylaminopurine (BAP: 2 mg/L) and 1-Naphtheleneacetic acid (NAA: 0.5 mg/L) were the successful hormonal combination. GC-MS analysis revealed the existence of nine key compounds in both in vitro and naturally grown L. cruciata. Result of antioxidant and anti-diabetic activity showed that in vitro grown L. cruciata has a more or less similar antioxidant and anti-diabetic activities as naturally grown ones. This result confirms the possibility of using in vitro grown plants in place of naturally grown plants for research and clinical purposes.
In vitro antioxidant and antidiabetic activity of oligopeptides derived from different mulberry (Morus alba L.) cultivars
Pharmacognosy Research
International Journal of Pharmacy and Pharmaceutical Sciences
Objective: To evaluate the effect of ultraviolet (UV) radiation on the therapeutical and oxidativ... more Objective: To evaluate the effect of ultraviolet (UV) radiation on the therapeutical and oxidative stress status of fenugreek seedlings during the post-germination phase.Methods: The seeds of Trigonella foenum-graecum L. were subjected to UV radiation of different wavelengths UV 254 nm, UV 365 nm and the combination of 254 nm and 365 nm UV during the course of germination up to 96 h. Free radicals scavenging activity, in vitro antidiabetic activity as well as total phenol (TPC) and flavonoid (TFC) contents were evaluated up to four days of germination stages. Analysis of total activities of superoxide dismutase (SOD), peroxidase, catalase and NADPH oxidase (NOX) was conducted using non-denaturing polyacrylamide gel electrophoresis along with histochemical detection of H2O2 and superoxide radical localisation, lipid peroxidation and plasma membrane integrity was also performed.Results: Overall it was observed that the sprouts exposed to UV 365 nm exhibited a significant enhancement i...
Asian Journal of Pharmaceutical and Clinical Research
Objective: This work was performed to evaluate the effect of priming with exogenous sources of ca... more Objective: This work was performed to evaluate the effect of priming with exogenous sources of calcium ion and nitric oxide on the antidiabeticactivity and the alkaloid contents of fenugreek sprouts along with isolation and identification of trigonelline, a bioactive alkaloid responsible for hypoglycemic property of fenugreek.Methods: The fenugreek seeds were pre-treated with calcium chloride (CC), lanthanum chloride (LC) a calcium channel blocker; ethylene glycol-bis (2-aminoethylether) -N, N, N´, N tetra acetic acid (EG) a calcium chelator; sodium nitroprusside (SNP) and 2-(4-carboxyphenyl) -4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (CP) a nitric oxide scavenger and germinated for 72 hrs. The sprout extracts were evaluated for their in vitro antidiabetic potential by α-amylase and α-glucosidase inhibition along with their trigonelline content. Trigonelline was isolated from fenugreek sprouts and identified by Infrared analysis and nuclear magnetic resonance (NMR) spectroscopy....
Tea Waste Management: A Case Study from West Bengal, India
Indian Journal of Science and Technology, 2016
Changes in Antioxidant Activity of Cyphomandra betacea (Cav.) Sendtn. Fruits During Maturation and Senescence
International Journal of Food Properties, 2011
ABSTRACT Antioxidants are extremely important substances which possess the ability to protect the... more ABSTRACT Antioxidants are extremely important substances which possess the ability to protect the body from damage caused by free radical induced oxidative stress. Antioxidants are derived from dietary sources like fruits, vegetables and beverages. In this study the antioxidant activity of different maturity stages of two varieties of Cyphomandra betacea fruits of Darjeeling were evaluated in vitro. The radical scavenging properties on 2,2 Diphenyl-1-picrylhydrazyl (DPPH), superoxide anion, hydroxyl radical, lipid peroxidation, nitric oxide and reducing power as well as the flavonoids, phenolics, lycopene and total carotene contents of methanolic extracts of the fruits were determined. All fruit extracts, mainly the mature red fruit of Purple-red variety exhibited strong scavenging activity towards all radicals tested due to the presence of relatively high total phenol, flavonoids, lycopene as well as total carotene contents. Our findings suggest that purple red variety of C. betacea fruit is endowed with antioxidant phytochemicals which could provide protection against oxidative stress induced diseases.
Phytochemical Screening and Antioxidant Activities of Two Selected ‘Bihi’ Fruits Used as Vegetables in Darjeeling Himalaya (Accepted)
In vitro detection of antioxidants in different solvent fractions of Ginger (Zingiber officinale Rosc.)
Indian Journal of Plant Physiology, 2009
... 23 Indian J. Plant Physiol., Vol. 14, No. 1, (NS) pp. 23-27 (Jan.-March, 2009) IN VITRO DETEC... more ... 23 Indian J. Plant Physiol., Vol. 14, No. 1, (NS) pp. 23-27 (Jan.-March, 2009) IN VITRO DETECTION OF ANTIOXIDANTS IN DIFFERENT SOLVENT FRACTIONS OF GINGER (ZINGIBER OFFICINALE ROSC.) MALAY BHATTACHARYA, PALASH MANDAL AND ARNAB SEN* ...
Antioxidant Activities of Different Parts of Tree Tomato Fruit (Accepted)
In Vitro Antioxidant Activity of Edible Timbur Fruits of Darjeeling Himalaya
In-Vitro free radical scavenging activities of the leaves of Malva verticillata L
Wheat (Triticum aestivum) peptide (s) mimic gibberellin action and regulate stomatal opening
Indian Journal of Experimental Biology, 2010
Wheat peptides (0.5 to 3 KDa M(r)) mimick hormonal activity like that of gibberellins and forced ... more Wheat peptides (0.5 to 3 KDa M(r)) mimick hormonal activity like that of gibberellins and forced open dark closed stomata. The deionized amphoteric peptides solution after passing through cation and anion exchanger resins was run through Amicon&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s ultrafilters, 10, 3 and 0.5 kDa (M(r)) cut off system. The 3 to 0.5 kDa fraction passed through sephadex LH-20 column and collected in 140 tubes (5 ml in each tube). The two fractions F 9 (91-100 tubes) and F 12 (121-130) were found much active on stomatal opening and a-amylase activity, respectively and were ninhydrin positive. Capillary electrophoresis of F 9 fraction yielded several peptides ranging 1600 to 2200 (M(r) and F 12 fraction showed 1800 - 2800(M(r)). Both the fractions were totally hydrolysed for amino acid analysis by HPLC. Most of the amino acids were present except cystein in both the fractions. The F 9 fraction, (peptide present in 10 microg fresh wt tissue per ml) induced the dark grown closed stomata to open upto 70%. In F 12 fraction, (peptide present in 10 microg fresh wt equivalent tissue per ml) showed alpha-amylase induction which was much higher than GA(3) (10(9) M). The peptide might be present in membrane and bound with GA that activated alpha-amylase m-RNA synthesis. The peptide might act directly on alpha-amylase gene.
There are about 622 species in the genus Litsea Lamarck (Lauraceae), distributed mainly in Austra... more There are about 622 species in the genus Litsea Lamarck (Lauraceae), distributed mainly in Australia, New Zealand, North & South America and Asia. Among those, 45 species of Litsea were reported from India. No comprehensive report is available on this genus from Terai and Duars regions, which are located at the foot of the Eastern Himalaya and located within the Himalaya Hotspot. Present paper reported the occurrence of nine species of Litsea from Terai and Duars belt along with their local names, salient features, exsiccatus, status, flowering and fruiting periods and geographic distribution. Collection of L. assamica Hooker f. from the Jaldapara National Park in Duars forms the new record for the state flora of West Bengal.
Eight different ferns collected from Mawsynram of the state of Meghalaya in India were evaluated ... more Eight different ferns collected from Mawsynram of the state of Meghalaya in India were evaluated for their in vitro antioxidant activity as well as associated phytochemical contents to explore the natural source of antioxidants. The in vitro antioxidant activity of the methanolic extract of the fern specimens were determined spectrophotometrically following DPPH, ABTS, ferric reducing power and metal chelating methods. Other phytochemical contents like phenol, flavonol and ortho-dihydric phenol were also evaluated following standard methodology. A positive correlation between the antioxidant activities and phytochemical contents was observed. Results demonstrated that out of eight evaluated ferns species, Aleuritopteris flava and Lindsaea odorata showed the best antioxidant activity in comparison to the methanolic extracts of other (Pteris scabristipes, Microlepia rhomboidea, Diplazium esculentum, Asplenium khasianum, Microlepia hallbergii and Adiantum edgeworthii) investigated ferns. The findings suggest that all these ferns are the good source of natural antioxidants and could be used as therapeutic agents in preventing the disorders associated with oxidative stress.