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Papers by PAZ ESPERANZA RODRIGUEZ PARDO

Research paper thumbnail of Vacinação Anti-Rábica Em Bovinos: Comparação De Cinco Esquemas Vacinais

Arquivos do Instituto Biológico

RESUMO A profilaxia antirrábica em bovinos no Brasil é baseada no uso de preparações vacinais de ... more RESUMO A profilaxia antirrábica em bovinos no Brasil é baseada no uso de preparações vacinais de vírus inativado. O Programa Brasileiro de Controle da Raiva Bovina indica o uso de uma dose destas vacinas (1 mL) seguida de um reforço. O objetivo deste trabalho foi avaliar e comparar os níveis de anticorpos induzidos por 5 esquemas alternativos de vacinação antirrábica em bovinos. Para isto, foram instituídos cinco grupos experimentais, denominados A, B, C, D e E, contendo 9 animais cada grupo. Os bovinos receberam, inicialmente, uma dose da vacina (dia 0). As doses de reforço foram aplicadas de acordo com o seguinte protocolo: grupo B (uma dose no dia 30); grupo C (uma dose no dia 180); os grupos D e E receberam duas doses de reforço, nos dias 30 e 60 e 30 e 180, respectivamente; o grupo A não recebeu dose de reforço. Amostras de sangue para obtenção de soro foram colhidas de todos os animais nos dias 0 (controle), 30, 60, 90, 180, 210 e 360 após o início da vacinação. Para aferir os...

Research paper thumbnail of Influência Da Suplementação Com Crômio Na Resposta Imune Humoral Anti-Rábica Em Bovinos

Arquivos do Instituto Biológico, 2006

RESUMO O objetivo do presente trabalho foi avaliar o efeito do crômio em diferentes concentrações... more RESUMO O objetivo do presente trabalho foi avaliar o efeito do crômio em diferentes concentrações na forma de levedura de crômio na resposta imune humoral anti-rábica em bovinos. Utilizaram-se 60 novilhas da raça Nelore (Bos indicus) com idade média de 2 e 5 anos, alimentadas em pastagem de Brachiaria decumbens, com fornecimento da água de bebida e sal mineral ad libidum, divididas aleatoriamente em 4 grupos de 15: grupo controle (Gc) que receberam o sal mineral sem crômio, os demais grupos receberam sal mineral contendo 8,5, 17 e 34 mg de crômio/kg de sal, grupos G8,5, G17 e G34, respectivamente. Todos os bovinos receberam no dia 0 uma dose de vacina comercial anti-rábica inativada. Colheram-se amostras de sangue nos dias 0, 30, 60 e 90 e os títulos de anticorpos anti-rábicos foram determinados por meio do teste de soroneutralização em camundongos. Os resultados obtidos mostram que as novilhas do grupo G17 apresentaram diferenças significativas no título de anticorpos anti-rábicos,...

Research paper thumbnail of Consideraciones actuales sobre el trabajo y su regulación jurídica

Research paper thumbnail of El Estatuto de los trabajadores y las reglas sobre conciliación de la vida laboral y familiar de los trabajadores. Un recorrido a través de veinticinco años

Derecho Social Y Relaciones Laborales Libro Homenaje a Joaquin Salvador Ruiz Melendez Y Francisco Trujillo Villanueva 2006 Isbn 84 611 0316 5 Pags 137 152, 2006

Research paper thumbnail of Construcción europea y protección de la salud (acerca de los residentes comunitarios en la Costa del Sol)

La Constitucion Europea Y Las Relaciones Laborales 2004 Isbn 84 96378 01 2 Pags 477 490, 2004

Research paper thumbnail of Effect of levamisole on the humoral immune response against rabies in cattle

BOVINE rabies, a fatal encephalitis caused by viruses of the genus Lyssavirus , is mainly transmi... more BOVINE rabies, a fatal encephalitis caused by viruses of the genus Lyssavirus , is mainly transmitted by the common vampire bat ( Desmodus rotundus ) ([Achkar and others 2007][1]). There is no effective treatment for rabies; therefore, regular vaccination of at-risk animals is the best control

Research paper thumbnail of Effects of primovaccination and booster vaccination on serum cortisol and humoral immune response in cattle

Advances in Bioscience and Biotechnology, 2013

The objective of this study was to evaluate the effect of one or two doses of the anti-rabies vac... more The objective of this study was to evaluate the effect of one or two doses of the anti-rabies vaccination on the serum concentration of cortisol and the humoral immune response in cattle as well as the correlation between serum cortisol concentrations and the titers of rabies-neutralizing antibodies. Nelore cattle were randomly assigned to one of three groups, which were vaccinated with one dose of rabies vaccine (group GVSR, N = 15), two doses of rabies vaccine (group GVR, N = 15) or were not vaccinated (group Gc, N = 15). A commercial liquid inactivated rabies vaccine was used. The stressors imposed on the cattle were vaccination, corral handling and the presence of people. Blood samples were collected on days 0, 30 and 60 post-vaccination. Serum cortisol concentrations were determined using a solid-phase radioimmunoassay, and rabies antibody titers were determined using a serum neutralization test with BHK 21 cells (RFFIT). Both serum cortisol concentrations and antibody titers increased after the second (booster) vaccination (P < 0.05). In all the groups, the serum cortisol concentrations increased after the cattle were handled in the corral (P < 0.05). No correlation was observed between the serum cortisol concentrations and the antibody titers with any treatment or on any observation day. In conclusion, booster vaccination is indispensable for primovaccinated cattle in achieving high and protective levels of rabies antibodies. Although booster vaccination and frequent cattle handling in corrals are stressors, the response is not strong enough to cause immunosuppression in cattle.

Research paper thumbnail of Suplementação nutricional na produção de anticorpos séricos contra o vírus rábico em ovinos vacinados contra raiva

Semina: Ciências Agrárias, 2014

Esse estudo avaliou o efeito da suplementação do probiótico com ou sem zinco (Zn), adicionado à m... more Esse estudo avaliou o efeito da suplementação do probiótico com ou sem zinco (Zn), adicionado à mistura mineral, na produção de anticorpos séricos contra o vírus rábico em ovinos vacinados com uma única dose de vacina. Quarenta e cinco ovinos machos, mestiços Santa Inês, com idade de 6 meses foram divididos aleatoriamente em 3 grupos (15 ovinos/grupo): Grupo controle (GC) recebeu 10 gramas de suplemento mineral/animal/dia, grupo probiótico (GP) recebeu 10 gramas de suplemento mineral adicionado 4 gramas de probiótico/animal/dia e grupo Probiótico e Zinco (GPZn) recebeu 10 gramas de suplemento mineral adicionado 4 gramas de probiótico e 14,4 mg de sulfato de zinco/ animal/dia adicionado ao probiótico. Os títulos individuais de anticorpos foram determinados por meio da técnica de soroneutralização baseado no Rapid Fluorescent Focus Inhibition Test (RFFIT) e no Fluorescent Inhibition Microtest (FIMT). Não houve diferenças estatísticas significativas entre as médias de concentrações séricas entre os grupos. Conclui-se que a administração de probióticos com ou sem zinco não melhora a resposta imune humoral antirrábica.

Research paper thumbnail of Utilização De Probióticos Sobre O Ganho De Peso Em Bovinos Da Raça Nelore

Colloquium Agrariae, 2013

This study evaluated the effect of probiotic proenzyme ®, added to the mineral mixture in a weigh... more This study evaluated the effect of probiotic proenzyme ®, added to the mineral mixture in a weight gain of cattle in extensive grazing system. We used 40 animals, Nelore bulls (Bos indicus) aged approximately 12 months, randomly divided into 2 groups (20 animals / group): control group (CG) received only mineral mixture and probiotic group (GP) with added probiotic. Calves were weighed on days 0 and 30 from 30 to 90 days and 00 to 90 days. The results showed a significant increase in weight gain in animals only GP in the first 30 days

Research paper thumbnail of Effects of probiotic supplementation on liveweight gain and serum cortisol concentration in cattle

Veterinary Record, 2011

PROBIOTICS are preparations from the culture of live microorganisms. They have been shown to have... more PROBIOTICS are preparations from the culture of live microorganisms. They have been shown to have beneficial effects when used as growth promoters (Ferreira and others 2009) and as immunostimulants (Arenas and others 2009) in calves. The aim of the present study was to evaluate the effect of a probiotic preparation added to the mineral mix given to young cattle with regard to liveweight gain and serum cortisol concentration. The study used 40 male Nelore cattle, approximately 18 months old, fed on Brachiaria brizantha under a rotational grazing system and divided into two experimental groups of 20 animals. Animals in the experimental group (probiotic group) were supplemented with 4.0 g/animal/day of probiotic (Proenzime; EMBRAUPEC), added to the mineral mix given to the cattle. The animals in the control group were supplemented with mineral mix without the addition of the probiotic. During the first 30 days, all cattle remained in their pasture …

Research paper thumbnail of Selenium supplementation and rabies antibody titres in cattle

Veterinary Record, 2008

SIR, — Regular vaccination is the most effective and inexpensive procedure for controlling rabies... more SIR, — Regular vaccination is the most effective and inexpensive procedure for controlling rabies in cattle ([Albas and others 2005][1]). Given that selenium (Se) enhances cattle immunity to infectious diseases ([Carroll and Forsberg 2007][2]), we investigated whether Se enhances the humoral

Research paper thumbnail of Matricaria chamomillaCH12decreases handling stress in Nelore calves

Journal of Veterinary Science, 2006

Research paper thumbnail of Association between handling stress in the corral and rabies antibody titers in selenium-supplemented cattle

Journal of Venomous Animals and Toxins including Tropical Diseases, 2009

This study determined the correlation between serum cortisol levels and rabies antibody titers in... more This study determined the correlation between serum cortisol levels and rabies antibody titers in cattle primo-vaccinated against rabies and supplemented with dietary selenium (Se). Sixty Nelore male calves (10 to 12 months old) received daily and individual dietary supplementation with 0, 3.6, 5.4 and 6.4 mg Se (groups Gc, G 3.6 , G 5.4 and G 6.4 , respectively). The animals were vaccinated against rabies (day 0) and subjected to handling stress in the corral for 120 days. Blood sampling procedures were performed on days 0, 15, 30, 60, 90 and 120. Cortisol levels increased until day 90, but had dropped significantly by day 120 (P < 0.01). Rabies antibody titers on days 30 and 90 were similar among Sesupplemented groups; in the control group, rabies antibodies decreased significantly from day 30 to 60, and 90 to 120. Serum cortisol levels and antibody titers were not correlated in most of the groups or blood sampling days. A positive correlation among these variables was found only in G 6.4 on days 60 (R = 0.513; P = 0.05) and 120 (R = 0.644; P = 0.009). In conclusion, repeated handling in the corral stresses cattle, but without compromising rabies humoral immune response.

Research paper thumbnail of Nuevas técnicas de docencia e investigación en Derecho del Trabajo y Seguridad Social: innovación educativa en el ámbito del Derecho Social (el enfoque didáctico …

… educativa y calidad …, 2006

Localización: Innovación educativa y calidad de la docencia universitaria: proyectos de innovació... more Localización: Innovación educativa y calidad de la docencia universitaria: proyectos de innovación educativa para la mejora de la práctica docente, año 2002/coord. por Francisco José González García, 2006, ISBN 84-9747-130-X, págs. 371-388

Research paper thumbnail of El proceso de Seguridad Social: balance y perspectivas

… Social a la luz de sus …, 2008

Información del artículo El proceso de Seguridad Social: balance y perspectivas.

Research paper thumbnail of Effects of selenium supplementation on serum cortisol in repeatedly handled cattle

Archivos de Zootecnia, 2012

Research paper thumbnail of Probiotic increase the antirabies humoral immune response in bovine

Archivos de Zootecnia, 2009

Research paper thumbnail of Efeito da suplementação com selênio sobre a concentração sérica de creatina kinase em bovinos

Archivos de Zootecnia, 2009

Research paper thumbnail of Evaluation of anti-rabies vaccination and supplementation with probiotic in the humoral immune response in cattle

Abstract: This study evaluated the humoral immune response of a new rabies vaccine developed by t... more Abstract: This study evaluated the humoral immune response of a new rabies vaccine developed by the Instituto Butantan (potency of 3.27 UI/ml) in primovaccinated cattle and the effect of probiotic on this response. Thirty-four 15-month old Nelore cattle were randomly ...

Research paper thumbnail of Vacinação Anti-Rábica Em Bovinos: Comparação De Cinco Esquemas Vacinais

Arquivos do Instituto Biológico

RESUMO A profilaxia antirrábica em bovinos no Brasil é baseada no uso de preparações vacinais de ... more RESUMO A profilaxia antirrábica em bovinos no Brasil é baseada no uso de preparações vacinais de vírus inativado. O Programa Brasileiro de Controle da Raiva Bovina indica o uso de uma dose destas vacinas (1 mL) seguida de um reforço. O objetivo deste trabalho foi avaliar e comparar os níveis de anticorpos induzidos por 5 esquemas alternativos de vacinação antirrábica em bovinos. Para isto, foram instituídos cinco grupos experimentais, denominados A, B, C, D e E, contendo 9 animais cada grupo. Os bovinos receberam, inicialmente, uma dose da vacina (dia 0). As doses de reforço foram aplicadas de acordo com o seguinte protocolo: grupo B (uma dose no dia 30); grupo C (uma dose no dia 180); os grupos D e E receberam duas doses de reforço, nos dias 30 e 60 e 30 e 180, respectivamente; o grupo A não recebeu dose de reforço. Amostras de sangue para obtenção de soro foram colhidas de todos os animais nos dias 0 (controle), 30, 60, 90, 180, 210 e 360 após o início da vacinação. Para aferir os...

Research paper thumbnail of Influência Da Suplementação Com Crômio Na Resposta Imune Humoral Anti-Rábica Em Bovinos

Arquivos do Instituto Biológico, 2006

RESUMO O objetivo do presente trabalho foi avaliar o efeito do crômio em diferentes concentrações... more RESUMO O objetivo do presente trabalho foi avaliar o efeito do crômio em diferentes concentrações na forma de levedura de crômio na resposta imune humoral anti-rábica em bovinos. Utilizaram-se 60 novilhas da raça Nelore (Bos indicus) com idade média de 2 e 5 anos, alimentadas em pastagem de Brachiaria decumbens, com fornecimento da água de bebida e sal mineral ad libidum, divididas aleatoriamente em 4 grupos de 15: grupo controle (Gc) que receberam o sal mineral sem crômio, os demais grupos receberam sal mineral contendo 8,5, 17 e 34 mg de crômio/kg de sal, grupos G8,5, G17 e G34, respectivamente. Todos os bovinos receberam no dia 0 uma dose de vacina comercial anti-rábica inativada. Colheram-se amostras de sangue nos dias 0, 30, 60 e 90 e os títulos de anticorpos anti-rábicos foram determinados por meio do teste de soroneutralização em camundongos. Os resultados obtidos mostram que as novilhas do grupo G17 apresentaram diferenças significativas no título de anticorpos anti-rábicos,...

Research paper thumbnail of Consideraciones actuales sobre el trabajo y su regulación jurídica

Research paper thumbnail of El Estatuto de los trabajadores y las reglas sobre conciliación de la vida laboral y familiar de los trabajadores. Un recorrido a través de veinticinco años

Derecho Social Y Relaciones Laborales Libro Homenaje a Joaquin Salvador Ruiz Melendez Y Francisco Trujillo Villanueva 2006 Isbn 84 611 0316 5 Pags 137 152, 2006

Research paper thumbnail of Construcción europea y protección de la salud (acerca de los residentes comunitarios en la Costa del Sol)

La Constitucion Europea Y Las Relaciones Laborales 2004 Isbn 84 96378 01 2 Pags 477 490, 2004

Research paper thumbnail of Effect of levamisole on the humoral immune response against rabies in cattle

BOVINE rabies, a fatal encephalitis caused by viruses of the genus Lyssavirus , is mainly transmi... more BOVINE rabies, a fatal encephalitis caused by viruses of the genus Lyssavirus , is mainly transmitted by the common vampire bat ( Desmodus rotundus ) ([Achkar and others 2007][1]). There is no effective treatment for rabies; therefore, regular vaccination of at-risk animals is the best control

Research paper thumbnail of Effects of primovaccination and booster vaccination on serum cortisol and humoral immune response in cattle

Advances in Bioscience and Biotechnology, 2013

The objective of this study was to evaluate the effect of one or two doses of the anti-rabies vac... more The objective of this study was to evaluate the effect of one or two doses of the anti-rabies vaccination on the serum concentration of cortisol and the humoral immune response in cattle as well as the correlation between serum cortisol concentrations and the titers of rabies-neutralizing antibodies. Nelore cattle were randomly assigned to one of three groups, which were vaccinated with one dose of rabies vaccine (group GVSR, N = 15), two doses of rabies vaccine (group GVR, N = 15) or were not vaccinated (group Gc, N = 15). A commercial liquid inactivated rabies vaccine was used. The stressors imposed on the cattle were vaccination, corral handling and the presence of people. Blood samples were collected on days 0, 30 and 60 post-vaccination. Serum cortisol concentrations were determined using a solid-phase radioimmunoassay, and rabies antibody titers were determined using a serum neutralization test with BHK 21 cells (RFFIT). Both serum cortisol concentrations and antibody titers increased after the second (booster) vaccination (P < 0.05). In all the groups, the serum cortisol concentrations increased after the cattle were handled in the corral (P < 0.05). No correlation was observed between the serum cortisol concentrations and the antibody titers with any treatment or on any observation day. In conclusion, booster vaccination is indispensable for primovaccinated cattle in achieving high and protective levels of rabies antibodies. Although booster vaccination and frequent cattle handling in corrals are stressors, the response is not strong enough to cause immunosuppression in cattle.

Research paper thumbnail of Suplementação nutricional na produção de anticorpos séricos contra o vírus rábico em ovinos vacinados contra raiva

Semina: Ciências Agrárias, 2014

Esse estudo avaliou o efeito da suplementação do probiótico com ou sem zinco (Zn), adicionado à m... more Esse estudo avaliou o efeito da suplementação do probiótico com ou sem zinco (Zn), adicionado à mistura mineral, na produção de anticorpos séricos contra o vírus rábico em ovinos vacinados com uma única dose de vacina. Quarenta e cinco ovinos machos, mestiços Santa Inês, com idade de 6 meses foram divididos aleatoriamente em 3 grupos (15 ovinos/grupo): Grupo controle (GC) recebeu 10 gramas de suplemento mineral/animal/dia, grupo probiótico (GP) recebeu 10 gramas de suplemento mineral adicionado 4 gramas de probiótico/animal/dia e grupo Probiótico e Zinco (GPZn) recebeu 10 gramas de suplemento mineral adicionado 4 gramas de probiótico e 14,4 mg de sulfato de zinco/ animal/dia adicionado ao probiótico. Os títulos individuais de anticorpos foram determinados por meio da técnica de soroneutralização baseado no Rapid Fluorescent Focus Inhibition Test (RFFIT) e no Fluorescent Inhibition Microtest (FIMT). Não houve diferenças estatísticas significativas entre as médias de concentrações séricas entre os grupos. Conclui-se que a administração de probióticos com ou sem zinco não melhora a resposta imune humoral antirrábica.

Research paper thumbnail of Utilização De Probióticos Sobre O Ganho De Peso Em Bovinos Da Raça Nelore

Colloquium Agrariae, 2013

This study evaluated the effect of probiotic proenzyme ®, added to the mineral mixture in a weigh... more This study evaluated the effect of probiotic proenzyme ®, added to the mineral mixture in a weight gain of cattle in extensive grazing system. We used 40 animals, Nelore bulls (Bos indicus) aged approximately 12 months, randomly divided into 2 groups (20 animals / group): control group (CG) received only mineral mixture and probiotic group (GP) with added probiotic. Calves were weighed on days 0 and 30 from 30 to 90 days and 00 to 90 days. The results showed a significant increase in weight gain in animals only GP in the first 30 days

Research paper thumbnail of Effects of probiotic supplementation on liveweight gain and serum cortisol concentration in cattle

Veterinary Record, 2011

PROBIOTICS are preparations from the culture of live microorganisms. They have been shown to have... more PROBIOTICS are preparations from the culture of live microorganisms. They have been shown to have beneficial effects when used as growth promoters (Ferreira and others 2009) and as immunostimulants (Arenas and others 2009) in calves. The aim of the present study was to evaluate the effect of a probiotic preparation added to the mineral mix given to young cattle with regard to liveweight gain and serum cortisol concentration. The study used 40 male Nelore cattle, approximately 18 months old, fed on Brachiaria brizantha under a rotational grazing system and divided into two experimental groups of 20 animals. Animals in the experimental group (probiotic group) were supplemented with 4.0 g/animal/day of probiotic (Proenzime; EMBRAUPEC), added to the mineral mix given to the cattle. The animals in the control group were supplemented with mineral mix without the addition of the probiotic. During the first 30 days, all cattle remained in their pasture …

Research paper thumbnail of Selenium supplementation and rabies antibody titres in cattle

Veterinary Record, 2008

SIR, — Regular vaccination is the most effective and inexpensive procedure for controlling rabies... more SIR, — Regular vaccination is the most effective and inexpensive procedure for controlling rabies in cattle ([Albas and others 2005][1]). Given that selenium (Se) enhances cattle immunity to infectious diseases ([Carroll and Forsberg 2007][2]), we investigated whether Se enhances the humoral

Research paper thumbnail of Matricaria chamomillaCH12decreases handling stress in Nelore calves

Journal of Veterinary Science, 2006

Research paper thumbnail of Association between handling stress in the corral and rabies antibody titers in selenium-supplemented cattle

Journal of Venomous Animals and Toxins including Tropical Diseases, 2009

This study determined the correlation between serum cortisol levels and rabies antibody titers in... more This study determined the correlation between serum cortisol levels and rabies antibody titers in cattle primo-vaccinated against rabies and supplemented with dietary selenium (Se). Sixty Nelore male calves (10 to 12 months old) received daily and individual dietary supplementation with 0, 3.6, 5.4 and 6.4 mg Se (groups Gc, G 3.6 , G 5.4 and G 6.4 , respectively). The animals were vaccinated against rabies (day 0) and subjected to handling stress in the corral for 120 days. Blood sampling procedures were performed on days 0, 15, 30, 60, 90 and 120. Cortisol levels increased until day 90, but had dropped significantly by day 120 (P < 0.01). Rabies antibody titers on days 30 and 90 were similar among Sesupplemented groups; in the control group, rabies antibodies decreased significantly from day 30 to 60, and 90 to 120. Serum cortisol levels and antibody titers were not correlated in most of the groups or blood sampling days. A positive correlation among these variables was found only in G 6.4 on days 60 (R = 0.513; P = 0.05) and 120 (R = 0.644; P = 0.009). In conclusion, repeated handling in the corral stresses cattle, but without compromising rabies humoral immune response.

Research paper thumbnail of Nuevas técnicas de docencia e investigación en Derecho del Trabajo y Seguridad Social: innovación educativa en el ámbito del Derecho Social (el enfoque didáctico …

… educativa y calidad …, 2006

Localización: Innovación educativa y calidad de la docencia universitaria: proyectos de innovació... more Localización: Innovación educativa y calidad de la docencia universitaria: proyectos de innovación educativa para la mejora de la práctica docente, año 2002/coord. por Francisco José González García, 2006, ISBN 84-9747-130-X, págs. 371-388

Research paper thumbnail of El proceso de Seguridad Social: balance y perspectivas

… Social a la luz de sus …, 2008

Información del artículo El proceso de Seguridad Social: balance y perspectivas.

Research paper thumbnail of Effects of selenium supplementation on serum cortisol in repeatedly handled cattle

Archivos de Zootecnia, 2012

Research paper thumbnail of Probiotic increase the antirabies humoral immune response in bovine

Archivos de Zootecnia, 2009

Research paper thumbnail of Efeito da suplementação com selênio sobre a concentração sérica de creatina kinase em bovinos

Archivos de Zootecnia, 2009

Research paper thumbnail of Evaluation of anti-rabies vaccination and supplementation with probiotic in the humoral immune response in cattle

Abstract: This study evaluated the humoral immune response of a new rabies vaccine developed by t... more Abstract: This study evaluated the humoral immune response of a new rabies vaccine developed by the Instituto Butantan (potency of 3.27 UI/ml) in primovaccinated cattle and the effect of probiotic on this response. Thirty-four 15-month old Nelore cattle were randomly ...