P. Conde - Academia.edu (original) (raw)
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Charité - Universitätsmedizin Berlin / Charité Medical University Berlin
CSIC (Consejo Superior de Investigaciones Científicas-Spanish National Research Council)
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Papers by P. Conde
Experientia, 1982
Experientia 38 (1982), Birkhfiuser Verlag, CH-4010 Basel/Switzerland 241 tion of fixative; perfus... more Experientia 38 (1982), Birkhfiuser Verlag, CH-4010 Basel/Switzerland 241 tion of fixative; perfusion of these small fish was not attempted. The brain was then quickly excised, placed in Rossmans' fixative precooled to -35 ~ and kept at that temperature for 24 h. Brains were then passed through 3 changes of ethanol at 4 ~ each of 1 h duration. Following this, specimens were cleared in xylene and embedded in wax. This method is designated 'experimental' in the table. Sections from all blocks were cut at 7 gin, floated on water (standard method) or alcohol (experimental method) and stained by methods listed in the table. Hydration was avoided wherever possible in 'experimental' methods. A brain was fixed for electron microscopy 17 while the fish was anaesthetized.
Experientia, 1982
Experientia 38 (1982), Birkhfiuser Verlag, CH-4010 Basel/Switzerland 241 tion of fixative; perfus... more Experientia 38 (1982), Birkhfiuser Verlag, CH-4010 Basel/Switzerland 241 tion of fixative; perfusion of these small fish was not attempted. The brain was then quickly excised, placed in Rossmans' fixative precooled to -35 ~ and kept at that temperature for 24 h. Brains were then passed through 3 changes of ethanol at 4 ~ each of 1 h duration. Following this, specimens were cleared in xylene and embedded in wax. This method is designated 'experimental' in the table. Sections from all blocks were cut at 7 gin, floated on water (standard method) or alcohol (experimental method) and stained by methods listed in the table. Hydration was avoided wherever possible in 'experimental' methods. A brain was fixed for electron microscopy 17 while the fish was anaesthetized.