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Papers by Philippe Lebacq
Experience acquise chez des porteurs asymptomatiques. La specificite des reactions positives par ... more Experience acquise chez des porteurs asymptomatiques. La specificite des reactions positives par ELISA est ensuite verifiee par Western-blot. Est traitee principalement la serologie permettant de reconnaitre les sujets infectes par HIV1 et sont abordees les nouvelles difficultes creees par la reconnaissance d'un 2eme type de virus responsable du SIDA, HIV2
Plant Science Letters, 1981
The Sal I restriction patterns of chloroplast (cp) and mitochondrial (rot) DNAs isolated from lea... more The Sal I restriction patterns of chloroplast (cp) and mitochondrial (rot) DNAs isolated from leaves of Brassica nigra, B. oleracea, B. campestris, B. carinata, B. juncea and B. napus were compared. In all cases, the Sal I patterns appeared simpler than those previously obtained from different genera. The cp DNA patterns of B. nigra, B. campestris, B. juncea and B. napus are similar containing only five restriction fragments. The molecular weight of these cp DNAs was estimated to be about 97 × 106 daltons. The small number of well separated mt DNA fragments allows for the first time the precise determination of the molecular weight of a higher plant mt DNA. Values ranging from 108.5 × 106 daltons with B. oleracea mt DNA to 138.5 × l0 s daltons with B. carinata mt DNA have been measured. The mt DNA Sal I patterns although supporting grossly the established taxonomic classification in Brassica, cannot indicate the direction of the original interspecific crosses. It is suggested that mt DNA sequences may have changed rapidly during the course of evolution and through sexual hybridization.
Bulletin de la Société Botanique de France. Actualités Botaniques, 1988
ABSTRACT Les plantes supérieures renferment 3 génomes. Les techniques très puissantes de biologie... more ABSTRACT Les plantes supérieures renferment 3 génomes. Les techniques très puissantes de biologie moléculaire permettent d'en étudier l'organisation moléculaire et l'expression avec une grande précision. Des méthodes d'analyse et de diagnostic, très fines et non destructives, sont disponibles et peuvent désormais être employées en routine.
Annales de biologie clinique, 1990
Nucleic acid probes represent an essential tool for molecular investigation in both basic and app... more Nucleic acid probes represent an essential tool for molecular investigation in both basic and applied research. In this last field they are particularly useful for diagnosis or prognosis and even for therapy. In the diagnosis, the nucleic probes theoretically allow to reach a sensitive level of reliability never obtained before. Nevertheless, the use of nucleic probes in diagnosis routine need to respect a drastic schedule of conditions since: nucleic probes have to be perfectly specific. nucleic probes have to be sensitive and to allow the detection of less than 100 femtogram of nucleic acid target in routine. In some cases this threshold has to be less than 1 femtogram (for instance HIV detection cases). Nucleic probes have to be absolutely reliable without risk to obtain false positives or negatives. Complete hybridization results must be obtained fast (within 2 or 3 hours in most cases). Labelling systems of nucleic acids must be non-isotopic. Numerous labelling systems, isotopi...
Annales de biologie clinique, 1992
Gene mutations responsible for main genetic diseases as Duchenne/Becker muscular dystrophy or cys... more Gene mutations responsible for main genetic diseases as Duchenne/Becker muscular dystrophy or cystic fibrosis, and involved in more important diseases like cancer or cardiac diseases have been identified. Direct DNA tests can now be performed for these disorders. However, despite the knowledge of the exact alteration of the DNA sequence in these diseases, incorporating DNA analysis into large screening programs has been hindered by technical difficulties since each different mutation requires a different probe to be detected. To overcome these problems the polymerase chain reaction technique (PCR) has been proposed. Multiplex PCR procedure is possible and consists of simultaneously amplifying several separate DNA sequences (the upper limit of the number of multiplex reactions that can be executed at one time is not known; eight or nine separate DNA sequences amplified have already been described). Another approach is to directly sequence the gene mutations generally concentrated in ...
Theoretical and Applied Genetics, 1980
Chloroplast (cp) and mitochondrial (mt) DNAs were isolated from four cereal genomes (cultivated w... more Chloroplast (cp) and mitochondrial (mt) DNAs were isolated from four cereal genomes (cultivated wheat, rye, barley and oats) and compared by restriction nuclease analysis. Cleavage of cp and mt DNAs by Sal I, Kpn I, Xho I and EcoR I enzymes indicated that each cereal group contains specific cytoplasmic DNAs. A phylogenetic tree of cereal evolution has been obtained on the basis of cp DNA homologies. It is suggested that wheat and rye diverged after their common ancestor had diverged from the ancestor of barley. This was preceded by the divergence of the common ancestor of wheat, rye and barley and the ancestor of oats. The molecular weight of the different cp DNAs was determined from the Sal I and Kpn I patterns, cp DNAs from wheat, rye, barley and oats appeared to be characterized by a very similar molecular weight of about 80-82.106 d. In the case of the mt DNAs, the great number of restriction fragments obtained with the restriction enzymes used prevented precise comparisons and determination of molecular weights.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1982
Chloroplast (cp) and mitochondrial (mt) compartments of normal (N) and cytoplasmic male sterile (... more Chloroplast (cp) and mitochondrial (mt) compartments of normal (N) and cytoplasmic male sterile (cms) lines of Brassica napus have been characterized and compared on the basis of cp and mt DNA restriction enzyme analysis and in vitro protein synthesis by isolated mitochondria. Cytoplasmic male sterility of B. napus (rape) comes from cms Raphanus sativus (radish) through intergeneric crosses.Cp DNAs isolated from N and cms lines had distinct restriction patterns with Sal I, Kpn I and Sma I enzymes. The size of the two cp DNAs measured from the restriction patterns was found to be identical and of about 95 × 10(6) d. N and cms lines of B. napus were characterized by specific mt DNAs, as shown from Sal I, Kpn I, Pst I and Xho I cleavage patterns. The small number of well-separated restriction fragments obtained with Sal I enabled us to determine precisely mt DNA sizes. The values of 136.5 and 140.3 × 10(6) d, obtained from restriction patterns with N and cms DNAs respectively, are smal...
Available from INIST (FR), Document Supply Service, under shelf-number : AR 16551 / INIST-CNRS - ... more Available from INIST (FR), Document Supply Service, under shelf-number : AR 16551 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueSIGLEMinistere de la Recherche et de la Technologie (MRT), 75 - Paris (France)FRFranc
DEVICE FOR DETECTION OF DNA AND RNA VIRAL GENOMES IN A SAMPLE OF A BIOLOGICAL MEDIUM THAT CAN BE ... more DEVICE FOR DETECTION OF DNA AND RNA VIRAL GENOMES IN A SAMPLE OF A BIOLOGICAL MEDIUM THAT CAN BE CONTAMINATED BY VIRAL PARTICLES. FIRST TREATED THE SAMPLE TO DIGERATE NUCLEIC ACIDS NON VIRAL DNA AND RNA AND PRESERVE VIRAL NUCLEIC ACIDS. ONCE YOU PURIFY THE SAMPLE, FOR EXAMPLE BY PASSING THROUGH A CARTRIDGE 32 CONTAINING A GEL TO RETAIN THE PROTEINS PRESENT IN THE SAMPLE. THE VIRAL NUCLEIC ACIDS ARE THEN EXTRACTED AND DENATURED IN THE PRESENCE OF A MIXTURE OF EXTRACTION IN A RESERVOIR 62. THEN THEY ARE COLLECTED AND THEN DETECTED BY AN APPROPRIATE PROCESS, FOR EXAMPLE BY A COLORIMETRIC PROCESS. This invention makes it possible to achieve, in a particularly simple manner, the polyprospective detection of DNA and RNA viral genomes, especially in biological media such as the serum.
The invention relates to a method for labeling a nucleic acid in a nucleic probe comprising a spe... more The invention relates to a method for labeling a nucleic acid in a nucleic probe comprising a specific nucleic-probe sequence to hybridize with a complementary target nucleic acid sequence, wherein the label of the nucleic acid is carried out by direct chemical bridging with a tracer system by means of a heterobifunctional compound. According to the invention, said labeled nucleic acid is a single-stranded vector, e.g., a M13 phage, comprising a circular single-stranded nucleic sequence. The invention also concerns a reagent kit for the implementation of this process.
Experience acquise chez des porteurs asymptomatiques. La specificite des reactions positives par ... more Experience acquise chez des porteurs asymptomatiques. La specificite des reactions positives par ELISA est ensuite verifiee par Western-blot. Est traitee principalement la serologie permettant de reconnaitre les sujets infectes par HIV1 et sont abordees les nouvelles difficultes creees par la reconnaissance d'un 2eme type de virus responsable du SIDA, HIV2
Plant Science Letters, 1981
The Sal I restriction patterns of chloroplast (cp) and mitochondrial (rot) DNAs isolated from lea... more The Sal I restriction patterns of chloroplast (cp) and mitochondrial (rot) DNAs isolated from leaves of Brassica nigra, B. oleracea, B. campestris, B. carinata, B. juncea and B. napus were compared. In all cases, the Sal I patterns appeared simpler than those previously obtained from different genera. The cp DNA patterns of B. nigra, B. campestris, B. juncea and B. napus are similar containing only five restriction fragments. The molecular weight of these cp DNAs was estimated to be about 97 × 106 daltons. The small number of well separated mt DNA fragments allows for the first time the precise determination of the molecular weight of a higher plant mt DNA. Values ranging from 108.5 × 106 daltons with B. oleracea mt DNA to 138.5 × l0 s daltons with B. carinata mt DNA have been measured. The mt DNA Sal I patterns although supporting grossly the established taxonomic classification in Brassica, cannot indicate the direction of the original interspecific crosses. It is suggested that mt DNA sequences may have changed rapidly during the course of evolution and through sexual hybridization.
Bulletin de la Société Botanique de France. Actualités Botaniques, 1988
ABSTRACT Les plantes supérieures renferment 3 génomes. Les techniques très puissantes de biologie... more ABSTRACT Les plantes supérieures renferment 3 génomes. Les techniques très puissantes de biologie moléculaire permettent d'en étudier l'organisation moléculaire et l'expression avec une grande précision. Des méthodes d'analyse et de diagnostic, très fines et non destructives, sont disponibles et peuvent désormais être employées en routine.
Annales de biologie clinique, 1990
Nucleic acid probes represent an essential tool for molecular investigation in both basic and app... more Nucleic acid probes represent an essential tool for molecular investigation in both basic and applied research. In this last field they are particularly useful for diagnosis or prognosis and even for therapy. In the diagnosis, the nucleic probes theoretically allow to reach a sensitive level of reliability never obtained before. Nevertheless, the use of nucleic probes in diagnosis routine need to respect a drastic schedule of conditions since: nucleic probes have to be perfectly specific. nucleic probes have to be sensitive and to allow the detection of less than 100 femtogram of nucleic acid target in routine. In some cases this threshold has to be less than 1 femtogram (for instance HIV detection cases). Nucleic probes have to be absolutely reliable without risk to obtain false positives or negatives. Complete hybridization results must be obtained fast (within 2 or 3 hours in most cases). Labelling systems of nucleic acids must be non-isotopic. Numerous labelling systems, isotopi...
Annales de biologie clinique, 1992
Gene mutations responsible for main genetic diseases as Duchenne/Becker muscular dystrophy or cys... more Gene mutations responsible for main genetic diseases as Duchenne/Becker muscular dystrophy or cystic fibrosis, and involved in more important diseases like cancer or cardiac diseases have been identified. Direct DNA tests can now be performed for these disorders. However, despite the knowledge of the exact alteration of the DNA sequence in these diseases, incorporating DNA analysis into large screening programs has been hindered by technical difficulties since each different mutation requires a different probe to be detected. To overcome these problems the polymerase chain reaction technique (PCR) has been proposed. Multiplex PCR procedure is possible and consists of simultaneously amplifying several separate DNA sequences (the upper limit of the number of multiplex reactions that can be executed at one time is not known; eight or nine separate DNA sequences amplified have already been described). Another approach is to directly sequence the gene mutations generally concentrated in ...
Theoretical and Applied Genetics, 1980
Chloroplast (cp) and mitochondrial (mt) DNAs were isolated from four cereal genomes (cultivated w... more Chloroplast (cp) and mitochondrial (mt) DNAs were isolated from four cereal genomes (cultivated wheat, rye, barley and oats) and compared by restriction nuclease analysis. Cleavage of cp and mt DNAs by Sal I, Kpn I, Xho I and EcoR I enzymes indicated that each cereal group contains specific cytoplasmic DNAs. A phylogenetic tree of cereal evolution has been obtained on the basis of cp DNA homologies. It is suggested that wheat and rye diverged after their common ancestor had diverged from the ancestor of barley. This was preceded by the divergence of the common ancestor of wheat, rye and barley and the ancestor of oats. The molecular weight of the different cp DNAs was determined from the Sal I and Kpn I patterns, cp DNAs from wheat, rye, barley and oats appeared to be characterized by a very similar molecular weight of about 80-82.106 d. In the case of the mt DNAs, the great number of restriction fragments obtained with the restriction enzymes used prevented precise comparisons and determination of molecular weights.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1982
Chloroplast (cp) and mitochondrial (mt) compartments of normal (N) and cytoplasmic male sterile (... more Chloroplast (cp) and mitochondrial (mt) compartments of normal (N) and cytoplasmic male sterile (cms) lines of Brassica napus have been characterized and compared on the basis of cp and mt DNA restriction enzyme analysis and in vitro protein synthesis by isolated mitochondria. Cytoplasmic male sterility of B. napus (rape) comes from cms Raphanus sativus (radish) through intergeneric crosses.Cp DNAs isolated from N and cms lines had distinct restriction patterns with Sal I, Kpn I and Sma I enzymes. The size of the two cp DNAs measured from the restriction patterns was found to be identical and of about 95 × 10(6) d. N and cms lines of B. napus were characterized by specific mt DNAs, as shown from Sal I, Kpn I, Pst I and Xho I cleavage patterns. The small number of well-separated restriction fragments obtained with Sal I enabled us to determine precisely mt DNA sizes. The values of 136.5 and 140.3 × 10(6) d, obtained from restriction patterns with N and cms DNAs respectively, are smal...
Available from INIST (FR), Document Supply Service, under shelf-number : AR 16551 / INIST-CNRS - ... more Available from INIST (FR), Document Supply Service, under shelf-number : AR 16551 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueSIGLEMinistere de la Recherche et de la Technologie (MRT), 75 - Paris (France)FRFranc
DEVICE FOR DETECTION OF DNA AND RNA VIRAL GENOMES IN A SAMPLE OF A BIOLOGICAL MEDIUM THAT CAN BE ... more DEVICE FOR DETECTION OF DNA AND RNA VIRAL GENOMES IN A SAMPLE OF A BIOLOGICAL MEDIUM THAT CAN BE CONTAMINATED BY VIRAL PARTICLES. FIRST TREATED THE SAMPLE TO DIGERATE NUCLEIC ACIDS NON VIRAL DNA AND RNA AND PRESERVE VIRAL NUCLEIC ACIDS. ONCE YOU PURIFY THE SAMPLE, FOR EXAMPLE BY PASSING THROUGH A CARTRIDGE 32 CONTAINING A GEL TO RETAIN THE PROTEINS PRESENT IN THE SAMPLE. THE VIRAL NUCLEIC ACIDS ARE THEN EXTRACTED AND DENATURED IN THE PRESENCE OF A MIXTURE OF EXTRACTION IN A RESERVOIR 62. THEN THEY ARE COLLECTED AND THEN DETECTED BY AN APPROPRIATE PROCESS, FOR EXAMPLE BY A COLORIMETRIC PROCESS. This invention makes it possible to achieve, in a particularly simple manner, the polyprospective detection of DNA and RNA viral genomes, especially in biological media such as the serum.
The invention relates to a method for labeling a nucleic acid in a nucleic probe comprising a spe... more The invention relates to a method for labeling a nucleic acid in a nucleic probe comprising a specific nucleic-probe sequence to hybridize with a complementary target nucleic acid sequence, wherein the label of the nucleic acid is carried out by direct chemical bridging with a tracer system by means of a heterobifunctional compound. According to the invention, said labeled nucleic acid is a single-stranded vector, e.g., a M13 phage, comprising a circular single-stranded nucleic sequence. The invention also concerns a reagent kit for the implementation of this process.