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Papers by Pablo Angel Sanchez Podlech

Research paper thumbnail of Semicontinuous lactic fermentation of whey byLactobacillus bulgaricus I. Experimental results

Biotechnology Letters, Jul 1, 1990

A Monod-like equation correlates the lactic acid productivity and the volume fraction of inoculum... more A Monod-like equation correlates the lactic acid productivity and the volume fraction of inoculum in semicontinuous fermentation of whey by Lactobacillus bulgaricus. The volume of the inoculum varied from 10% to 80% of the reactor working volume.

Research paper thumbnail of Kinetics of semicontinuous microbial transformation of whey by Lactobacillus bulgaricus varying the initial concentration of yeast autolysate

Journal of Biotechnology, 1993

Semicontinuous tests of microbial transformation of whey by Lactobacillus bulgaricus were carried... more Semicontinuous tests of microbial transformation of whey by Lactobacillus bulgaricus were carried out using yeast autolysate as nutrient. The initial concentration of yeast autolysate varied from 1.0 to 1.6 g 1-1. The medium pH was controlled by the addition of NH4OH solution. The volume of the inoculum varied from 20% to 50% of the bioreactor working volume. A mathematical model is proposed to explain the influence of the volume fraction of inoculum on the bioprocess time and, consequently, on the lactic acid productivity. Semicontinuous bioprocess; Microbial transformation of whey; Microbial process kinetics

Research paper thumbnail of Comparação do crescimento de Saccharomyces boulardii em fermentador por batelada tipo air lift e shaker

Ciência e Tecnologia de Alimentos, 2007

Os fermentadores tipo air lift oferecem vantagens tais como: eficiente homogeneização dos compone... more Os fermentadores tipo air lift oferecem vantagens tais como: eficiente homogeneização dos componentes, baixo cisalhamento e economia de energia, pois o meio é agitado pelo processo de aeração, sem necessidade de agitação mecânica. O objetivo deste trabalho foi analisar a cinética de crescimento de Saccharomyces boulardii neste fermentador, com aeração de 1 e 1,5 vvm (volume de ar por volume de meio, por minuto), comparada com o crescimento em frascos agitados em shaker, visando a futura aplicação deste fermentador, em escala industrial. Os resultados indicaram que houve uma diminuição do pH com o consumo da glicose do meio, a qual foi totalmente consumida até o final da fase exponencial, de 5 e 6 horas para o shaker e o air lift, respectivamente. Após este período houve uma alteração na velocidade de crescimento de S. boulardii, em ambos os equipamentos, indicando uma possível mudança na fonte de carbono utilizada, uma vez que toda a glicose foi consumida após estes períodos. Os valores de velocidades específicas de crescimento foram semelhantes para o shaker e airlift com 1,0 vvm, porém inferiores ao airlift com 1,5 vvm, indicando que neste último reator há possibilidades de se conseguir uma velocidade de produção celular maior, dependendo apenas da eficiência de oxigenação oferecida.

Research paper thumbnail of Morphological changes ofLactobacillus bulgaricus during steady-state continuous fermentation of whey

Biotechnology Letters, 1989

Summary During steady-state continuous fermentation of whey,Lactobacillus bulgaricus cells were o... more Summary During steady-state continuous fermentation of whey,Lactobacillus bulgaricus cells were observed to undergo several morphological changes without affecting the system performance.

Research paper thumbnail of An empirical correlation between the oil drop size distribution in hydrocarbon-water systems, oil concentration, and impeller speed

Biotechnology and Bioengineering, 1976

Research paper thumbnail of Oil drop size distribution in hydrocarbon-water systems

Biotechnology and Bioengineering, 1972

In this work, a radiometric method is used for the determination of the oil drop size distributio... more In this work, a radiometric method is used for the determination of the oil drop size distribution in agitated hydrocarbon-water systems. The influence of the counter position, the oil concentration, and the rotation speed of impeller were studied. An experimental parameter is proposed for the definition of the drop size distribution. It was observed that an unsymmetrical distribution represents the drop size distribution better than the normal distribution law.

Research paper thumbnail of Semicontinuous lactic fermentation of whey by Lactobacillus bulgaricus. II. Mathematical model

Biotechnology Letters, 1990

Research paper thumbnail of Continuous fermentation process for the production of bacterial cellulose film

Research paper thumbnail of Process for obtaining a ccellulosic wet sheet and a membrane, the equipment used to obtain the membrane and the membrane obtained

Research paper thumbnail of Continuous Fermentation Process to Produce Bacterial Cellulosic Sheets

Research paper thumbnail of A Process for Obtaining a Cellulosic Wet Sheet and a Membrane, the Equipment Used to Obtain the Membrane and the Membrane Obtained

Research paper thumbnail of Radiochemical Control of Radio Pharmaceuticals: Thin-Layer Chromatography

Research paper thumbnail of A radiometric method for determination of the oil drop size distribution in hydrocarbon-water systems

Biotechnology and Bioengineering, 1972

... uate b (Fig. 3): log Yo = 2 100 = Yo . 10-a' .*. b = a and we may substitute eq. ... WAL... more ... uate b (Fig. 3): log Yo = 2 100 = Yo . 10-a' .*. b = a and we may substitute eq. ... WALTER BORZANI Biochemical Engineering Laboratory Department of Chemical Engineering Escola Polit6cnica University of S&o Paul0 S&Q Paulo, Brazil PABLO A. SANCHEZ PODLECH ...

Research paper thumbnail of Efeito de inoculante ectomicorrízico produzido por fermentação semi-sólida sobre o crescimento de Eucalyptus dunnii Maiden

Pesquisa …, 2001

Pesq. agropec. bras., Brasília, v. 36, n. 2, p. 307-313, fev. 2001 ... Efeito de inoculante ectom... more Pesq. agropec. bras., Brasília, v. 36, n. 2, p. 307-313, fev. 2001 ... Efeito de inoculante ectomicorrízico produzido por fermentação semi-sólida ... Efeito de inoculante ectomicorrízico produzido por fermentação semi-sólida sobre o crescimento de Eucalyptus dunnii Maiden(1)

Research paper thumbnail of Comparação do crescimento de Saccharomyces boulardii em fermentador por batelada tipo air lift e shaker

Ciencia E Tecnologia De Alimentos, 2007

This work aimed to analyze the kinetics of S. boulardii growth in an air lift fermentor under two... more This work aimed to analyze the kinetics of S. boulardii growth in an air lift fermentor under two aeration conditions: 1 and 1.5 air vvm (air volume per volume of culture medium per minute), in comparison with growth in shaken flasks, with a view to the future application of this fermenter on an industrial scale. Air lift fermentors offer advantages such as efficient homogenization of the components, low shear stress, and energy savings because the medium is stirred by aeration and does not require mechanical stirring. The pH of the medium decreased with the glucose uptake during the exponential phase, probably due to the formation of secondary metabolites. The entire process showed a similar specific growth rate during the exponential phase (up to 5 hours in the case of the shaker and 6 hours in that of the air-lift fermentor), indicating a possible change in the carbon source, since all the glucose was depleted after the aforementioned periods. After 6 hours, the growth rates in t...

Research paper thumbnail of Semicontinuous lactic fermentation of whey byLactobacillus bulgaricus I. Experimental results

Biotechnology Letters, Jul 1, 1990

A Monod-like equation correlates the lactic acid productivity and the volume fraction of inoculum... more A Monod-like equation correlates the lactic acid productivity and the volume fraction of inoculum in semicontinuous fermentation of whey by Lactobacillus bulgaricus. The volume of the inoculum varied from 10% to 80% of the reactor working volume.

Research paper thumbnail of Kinetics of semicontinuous microbial transformation of whey by Lactobacillus bulgaricus varying the initial concentration of yeast autolysate

Journal of Biotechnology, 1993

Semicontinuous tests of microbial transformation of whey by Lactobacillus bulgaricus were carried... more Semicontinuous tests of microbial transformation of whey by Lactobacillus bulgaricus were carried out using yeast autolysate as nutrient. The initial concentration of yeast autolysate varied from 1.0 to 1.6 g 1-1. The medium pH was controlled by the addition of NH4OH solution. The volume of the inoculum varied from 20% to 50% of the bioreactor working volume. A mathematical model is proposed to explain the influence of the volume fraction of inoculum on the bioprocess time and, consequently, on the lactic acid productivity. Semicontinuous bioprocess; Microbial transformation of whey; Microbial process kinetics

Research paper thumbnail of Comparação do crescimento de Saccharomyces boulardii em fermentador por batelada tipo air lift e shaker

Ciência e Tecnologia de Alimentos, 2007

Os fermentadores tipo air lift oferecem vantagens tais como: eficiente homogeneização dos compone... more Os fermentadores tipo air lift oferecem vantagens tais como: eficiente homogeneização dos componentes, baixo cisalhamento e economia de energia, pois o meio é agitado pelo processo de aeração, sem necessidade de agitação mecânica. O objetivo deste trabalho foi analisar a cinética de crescimento de Saccharomyces boulardii neste fermentador, com aeração de 1 e 1,5 vvm (volume de ar por volume de meio, por minuto), comparada com o crescimento em frascos agitados em shaker, visando a futura aplicação deste fermentador, em escala industrial. Os resultados indicaram que houve uma diminuição do pH com o consumo da glicose do meio, a qual foi totalmente consumida até o final da fase exponencial, de 5 e 6 horas para o shaker e o air lift, respectivamente. Após este período houve uma alteração na velocidade de crescimento de S. boulardii, em ambos os equipamentos, indicando uma possível mudança na fonte de carbono utilizada, uma vez que toda a glicose foi consumida após estes períodos. Os valores de velocidades específicas de crescimento foram semelhantes para o shaker e airlift com 1,0 vvm, porém inferiores ao airlift com 1,5 vvm, indicando que neste último reator há possibilidades de se conseguir uma velocidade de produção celular maior, dependendo apenas da eficiência de oxigenação oferecida.

Research paper thumbnail of Morphological changes ofLactobacillus bulgaricus during steady-state continuous fermentation of whey

Biotechnology Letters, 1989

Summary During steady-state continuous fermentation of whey,Lactobacillus bulgaricus cells were o... more Summary During steady-state continuous fermentation of whey,Lactobacillus bulgaricus cells were observed to undergo several morphological changes without affecting the system performance.

Research paper thumbnail of An empirical correlation between the oil drop size distribution in hydrocarbon-water systems, oil concentration, and impeller speed

Biotechnology and Bioengineering, 1976

Research paper thumbnail of Oil drop size distribution in hydrocarbon-water systems

Biotechnology and Bioengineering, 1972

In this work, a radiometric method is used for the determination of the oil drop size distributio... more In this work, a radiometric method is used for the determination of the oil drop size distribution in agitated hydrocarbon-water systems. The influence of the counter position, the oil concentration, and the rotation speed of impeller were studied. An experimental parameter is proposed for the definition of the drop size distribution. It was observed that an unsymmetrical distribution represents the drop size distribution better than the normal distribution law.

Research paper thumbnail of Semicontinuous lactic fermentation of whey by Lactobacillus bulgaricus. II. Mathematical model

Biotechnology Letters, 1990

Research paper thumbnail of Continuous fermentation process for the production of bacterial cellulose film

Research paper thumbnail of Process for obtaining a ccellulosic wet sheet and a membrane, the equipment used to obtain the membrane and the membrane obtained

Research paper thumbnail of Continuous Fermentation Process to Produce Bacterial Cellulosic Sheets

Research paper thumbnail of A Process for Obtaining a Cellulosic Wet Sheet and a Membrane, the Equipment Used to Obtain the Membrane and the Membrane Obtained

Research paper thumbnail of Radiochemical Control of Radio Pharmaceuticals: Thin-Layer Chromatography

Research paper thumbnail of A radiometric method for determination of the oil drop size distribution in hydrocarbon-water systems

Biotechnology and Bioengineering, 1972

... uate b (Fig. 3): log Yo = 2 100 = Yo . 10-a' .*. b = a and we may substitute eq. ... WAL... more ... uate b (Fig. 3): log Yo = 2 100 = Yo . 10-a' .*. b = a and we may substitute eq. ... WALTER BORZANI Biochemical Engineering Laboratory Department of Chemical Engineering Escola Polit6cnica University of S&o Paul0 S&Q Paulo, Brazil PABLO A. SANCHEZ PODLECH ...

Research paper thumbnail of Efeito de inoculante ectomicorrízico produzido por fermentação semi-sólida sobre o crescimento de Eucalyptus dunnii Maiden

Pesquisa …, 2001

Pesq. agropec. bras., Brasília, v. 36, n. 2, p. 307-313, fev. 2001 ... Efeito de inoculante ectom... more Pesq. agropec. bras., Brasília, v. 36, n. 2, p. 307-313, fev. 2001 ... Efeito de inoculante ectomicorrízico produzido por fermentação semi-sólida ... Efeito de inoculante ectomicorrízico produzido por fermentação semi-sólida sobre o crescimento de Eucalyptus dunnii Maiden(1)

Research paper thumbnail of Comparação do crescimento de Saccharomyces boulardii em fermentador por batelada tipo air lift e shaker

Ciencia E Tecnologia De Alimentos, 2007

This work aimed to analyze the kinetics of S. boulardii growth in an air lift fermentor under two... more This work aimed to analyze the kinetics of S. boulardii growth in an air lift fermentor under two aeration conditions: 1 and 1.5 air vvm (air volume per volume of culture medium per minute), in comparison with growth in shaken flasks, with a view to the future application of this fermenter on an industrial scale. Air lift fermentors offer advantages such as efficient homogenization of the components, low shear stress, and energy savings because the medium is stirred by aeration and does not require mechanical stirring. The pH of the medium decreased with the glucose uptake during the exponential phase, probably due to the formation of secondary metabolites. The entire process showed a similar specific growth rate during the exponential phase (up to 5 hours in the case of the shaker and 6 hours in that of the air-lift fermentor), indicating a possible change in the carbon source, since all the glucose was depleted after the aforementioned periods. After 6 hours, the growth rates in t...