Pankaj Shrivastava - Academia.edu (original) (raw)

Papers by Pankaj Shrivastava

99, 2021

The extremely high nature of transmissibility and severity of infection due to the novel Corona v... more The extremely high nature of transmissibility and severity of infection due to the novel Corona virus is a serious threat to mankind. This letter delivers a caution for forensic DNA experts in the era of COVID-19 infection from the Indian perspective. Samples are routinely transported to laboratories without any specific guidelines. Therefore, this is high time to formulate clear guidelines for the handling of biological material, from receiving to processing in the laboratory during such a pandemic

Springer Protocols Handbooks, 2020

International Journal of Legal Medicine, 2021

In order to find out the genetic structure and characterize the forensic features of 23 autosomal... more In order to find out the genetic structure and characterize the forensic features of 23 autosomal STRs in the population of Assam, PowerPlex® Fusion 6C amplification kit was employed to genotype 292 unrelated individuals from Assam, India. A total of 79 different alleles were observed across 23 autosomal STRs with the corresponding frequency of alleles, which ranges from 0.002 to 0.406. Selected 23 loci were observed useful together with the highest value of combined discrimination power (CPD = 1), combined exclusion power (CPE = 0.999999999914), combined paternity index (CPI = 3.04 × 109), and all studied loci combined showing the lowest matching probability which is (CPM = 1.29 × 10−29). Additionally, in comparison with neighboring population, Assam population showed genetic closeness with Indian population. The genetic data of the present study will enrich the existing Indian autosomal DNA database and be useful for forensic and genealogical applications.

Annals of Human Biology, 2021

Forensic Science International: Genetics Supplement Series, 2019

The present investigation assessed the allele frequencies and forensic parameters for the loci in... more The present investigation assessed the allele frequencies and forensic parameters for the loci included in PowerPlex 21 multiplex system (Promega, USA) in 101 unrelated individuals residing in the state of Gujarat, India. The study likewise introduces the first global report on polymorphism on Penta D and Penta E autosomal STR loci from the population of Gujarat, India. The obtained outcomes in the study revealed that the considered STR multiplex system is highly polymorphic and suitable for forensic genetic analysis.

Choice Reviews Online, 1997

... NCJ Number: 174558. Title: Introduction to Forensic DNA Analysis. Author(s): K Inman; N Rudin... more ... NCJ Number: 174558. Title: Introduction to Forensic DNA Analysis. Author(s): K Inman; N Rudin. Sale: CRC Press LLC 2000 Corporate Blvd . ...

Ever since the inception of STR-based forensic DNA analysis for human Identification and DNA prof... more Ever since the inception of STR-based forensic DNA analysis for human Identification and DNA profiling, the approach developed continuously. Most of these developments are pertaining to achieve the best polymorphic loci set for analysis, high inhibitor tolerance capability, increase in sensitivity, reduction in turnaround time, and cost-effectiveness. On the similar line, the SF25TM PCR amplification kit, a 6 fluorescent dye-chemistry based autosomal STR kit was developed, which analyses 25 STR loci viz., FAM fluorescent dye-labelled D3S1358, D13S317, D7S820, D16S539, D1S1656, and Penta E; HEX fluorescent dye-labelled TPOX, TH01, D2S1338, CSF1PO, and Penta D; SUM fluorescent dye-labeled D19S433, vWA, D21S11, D18S51, and D6S1043; LYN fluorescent dye-labelled Amelogenin, D8S1179, D5S818, D12S391, and FGA; PUR fluorescent dye-labelled D22S1045, Y indel; D2S441 and D10S1248, The developmental validation of the SF25 PCR Amplification reagents were carried out in this study to analyze its...

BMC Research Notes, 2021

Objective This study was planned to evaluate the genetic diversity in the admixed and Teli (a Hin... more Objective This study was planned to evaluate the genetic diversity in the admixed and Teli (a Hindu caste) populations of Maharashtra, India using 20 autosomal Short Tandem Repeat (STR) genetic markers. We further investigated the genetic relatedness of the studied populations with other Indian populations. Results The studied populations showed a wide range of observed heterozygosity viz. 0.690 to 0.918 for the admixed population and 0.696 to 0.942 for the Teli population. This might be due to the multi-directional gene flow. The admixed and Teli populations also showed a high degree polymorphism which ranged from 0.652 to 0.903 and 0.644 to 0.902, respectively. Their combined value of matching probability for all the studied loci was 4.29 × 10–25 and 5.01 × 10–24, respectively. The results of Neighbor-Joining tree and Principal Component Analysis showed that the studied populations clustered with the general populations of Jharkhand, UttarPradesh, Rajasthan and Central Indian Stat...

Abstract- Every individual in the world can be, identified at the molecular level on the basis of... more Abstract- Every individual in the world can be, identified at the molecular level on the basis of an extremely high level of polymorphism in the sequence of his or her DNA, which he or she inherits from his or her biological parents and is Identical in every cell of the body. DNA fingerprinting, as this technique of identification is called, can confirm with certainty the parentage of an individual. The application of DNA profiling in the criminal justice system is an important issue in criminal investigators today. The technology is changing rapidly and several new techniques are becoming available. DNA profiling has been described as a powerful breakthrough in forensic science. The Forensic Use of DNA Profiling is a major contribution to a technology which can help not only in including the culprit but also to exclude the innocent. In this article an attempt is made to elaborate the changing scenario of the technology in the recent years as well as to present the some real cases w...

Romanian Journal of Legal Medicine, 2017

The potential of microorganisms is becoming increasingly apparent in forensic investigations as a... more The potential of microorganisms is becoming increasingly apparent in forensic investigations as a consequence of advances in molecular sciences and genomics. After the advent of DNA technology in forensics various attempts have been made towards the betterment of the technology by increasing number of loci, to cope up with PCR inhibitors and a bit with low copy or degraded DNA. Still, the work on presence of microbial flora as the signature flora and their possible role in degradation of body fluids need to be worked out. The bacteria are known degraders of organic molecules, but the bacterial flora of forensic samples and their possible role in degradation of body fluid has not with been studied so far. This paper is an attempt to revisit this issue and to characterize a few bacterial isolates from real forensic case exhibits. This is the first report on isolation and molecular characterization of bacteria from the blood stained forensic case exhibits.

kinship analysis in forensic is based on calculation of respective kinship indices. But calculati... more kinship analysis in forensic is based on calculation of respective kinship indices. But calculation of the same is possible only when the subject under identification has been associated to a particular population whose gene frequency data is available for the particular set of markers used in forensic practices. In case of the mass disasters where a huge number of individuals are to be identified, gathering the population frequency data and calculating the kinship indices can be an intricate progression requiring a lot of time and huge resources. The present study is based on allele matching score values which doesn’t require the use of allele frequency data to establish kinship. This method is based on the allele sharing approach which simply refers to the number of shared alleles (1 or 2) between two individuals; also known as identical by state (IBS) alleles which might have been inherited from a recent common ancestor in which the alleles are identical by descendent (IBD). In c...

Springer Protocols Handbooks, 2020

A natural geographical barrier between Tibetan plateau and south Asian countries, the Himalayan M... more A natural geographical barrier between Tibetan plateau and south Asian countries, the Himalayan Mountain range, serves as a natural barrier between these regions for genetic flow. This has lead to vast genomic divergence among the population residing in Indian Himalayan region..This study was designed with an aim to decipher the genomic diversity and molecular characterization of 21 hypervariable molecular markers in the population of geographical province of Himachal Pradesh in the lower Himalayan region. Randomly selected 401 unrelated individuals native of the lower Himalayan geographical region were included for this study. 21 hypervariable molecular markers included in the PowerPlex® 21 system were amplified and genotyped. A total of 246 alleles and 12.3 (SE 0.927) private alleles per locus were observed. Population differentiation analysis revealed that the studied population showed a genetic affinity with the population of North India, North-west India, Central India, and Utt...

International Journal of Legal Medicine, 2021

Introduction Forensic characterization and genetic evaluation study in the 539 randomly selected ... more Introduction Forensic characterization and genetic evaluation study in the 539 randomly selected unrelated adult healthy individuals belonging to the Central Indian population was undertaken. Methods The study was performed using a multiplex of 27 Y-STRs incoporated in Yfiler™ Plus multiplex kit. Results Out of 539 samples, 6 samples were observed for large deletion and tri-allelic patterns, which were removed from the analysis, and out of 533 samples, a total of 507 haplotypes were found, and out of these haplotypes, 482 unique haplotypes were found in this piece of work. The forensically important parameters, i.e., gene diversity (GD) and discrimination capacity (DC), were found to be 0.669 and 0.951, respectively, for the tested Y STR loci. The genetic data of this study will enrich the Y STR data bank and being used as a potential tool for forensic DNA and various genetic studies.