Pannapa Powthong - Academia.edu (original) (raw)

Papers by Pannapa Powthong

Journal of Agricultural Science and Technology, Dec 1, 2013

The purpose of this research was to study the antimicrobial activities of the endophytic fungi fr... more The purpose of this research was to study the antimicrobial activities of the endophytic fungi from Sesbania grandiflora (L.) Pers. The endophytic fungi were isolated from branches and leaves of Sesbania grandiflora (L.) Pers., and sixty nine isolates were obtained. All isolates were screened for antibacterial and antifungal activities. The indicator organisms were 4 bacteria including Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853, two yeasts, namely, Candida albicans and Cryptococcus neoformans, and 6 molds including Rhizopus spp., Mucor spp., Penicillium spp., Aspergillus spp., Curvularia spp., and Alternaria spp.. It was found that 28 and 16 isolates showed antibacterial activity against gram positive and gram negative bacteria, respectively. Also, 11 and 17 isolates showed antifungal activity against yeasts and hyaline non-septate hyphae, respectively. Besides, 13 and 65 isolates showed antifungal activity against hyaline septate and dematiaceous, respectively. There were 9 isolates that could inhibit bacteria, yeast, and molds. Macroscopic and microscopic examination of the fungal morphology revealed that most of the endophytic fungi (25 isolates) were hyaline septate hyphae. Only 3 isolates were hyaline non-septate hyphae. Fusarium spp. and Acremonium spp. were the predominate species among the isolated endophytic fungi. These results indicated that some endophytic fungi isolated from Sesbania grandiflora (L.) Pers. were potential sources of antimicrobial compounds against the tested bacteria, yeasts, and molds.

Asian Journal of Pharmaceutical and Clinical Research, Sep 1, 2017

Objective: The objective of this investigation was to assess the biological activity of fresh fru... more Objective: The objective of this investigation was to assess the biological activity of fresh fruit juices and freeze dried fruit juices including antioxidant and antibacterial activity. Methods: The fruits used in this study were Schleichera oleosa (Lour.) Oken, Carissa carandas L., and Sandoricum koetjape (Burm.f.) Merr. The fresh juice sample (FJS) was extracted and also prepared for freeze drying sample (FDS) by freeze dryer. The antioxidant capacity of FJS and FDS was assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays and determination for total phenolic contents (TPCs) by Folin-Ciocalteu's reagent. Antimicrobial tests were carried out by agar diffusion methods and evaluated by measuring the zone of inhibition against 10 isolates of pathogenic bacteria. Results: The highest antioxidant activity of FJS and FDS was found in S. oleosa and related to the scavenging effect on DPPH radical of FJS (100% concentration) and FDS (200 mg/ml) was found in the value of 93.05±0.31% and 82.18±0.33%, respectively. TPC of FJS and FDS from S. oleosa at the same concentration as in the DPPH assay was 1,003.53±1.96 ug GAE/300 g of matter and 827.77±23.15 ug GAE/100 ml of matter, respectively. Both FJS and FDS revealed antibacterial activity. The zone of inhibition of FJS was ranging from 8.3±0.6 to 24.0±1.0 mm and FDS values of 7.3±0.5-21.0±0.9 mm. The most potent sample against bacteria was FJS and FDS from S. oleosa which FJSs against Escherichia coli ESBL and FDS against Pseudomonas aeruginosa MDR2. Conclusion: Fruit juices of S. oleosa, C. carandas, and S. koetjape associated with antioxidant activity in the form of both fresh fruit juices and FDS. The result of this study showed that the value of DPPH and TPCs were slightly decreased in FDS compare with fresh juice. Screening of FJS and FDS of C. carandas revealed broad spectrum antibacterial activity. However, FJS of S. oleosa and S. koetjape showed inhibition of growth of bacteria, but few of FDS from these fruits could inhibit limited bacterial isolates. For antibacterial activity against pathogenic bacteria, the samples of fresh and freeze dried exhibited a different degree of activity.

Recent Patents on Food, Nutrition & Agriculture, Dec 31, 2021

Background: Salad vegetables are good sources of dietary fiber and are becoming increasingly popu... more Background: Salad vegetables are good sources of dietary fiber and are becoming increasingly popular among consumers. Therefore, these plants have the potential to be developed as functional foods. Objective: Using an in vitro model, this study investigated the physical properties and intestinal glucose and lipid absorption capacities of dry dietary fiber from vegetables typically consumed in salads (types of lettuce, including red oak, red coral, green oak, butterhead, and cos). Method: Fiber was prepared from each type of lettuce using an enzymatic method and then characterized. Physical properties, including solubility and water-binding, swelling, cation-exchange, and oil-binding capacities, and antihyperglycemic and antihypercholesterolemic effects of fiber were investigated. Results: The hydration capacity of total dietary fiber and insoluble fiber from the majority of sources was significantly different from that of cellulose. Adsorption and diffusion of glucose were directly proportional to incubation time, and the diffusion rate was significantly lower in the treatments containing fiber compared to the cellulose control. Fiber from these vegetables also inhibited amylase and alpha-glucosidase activities. Moreover, fiber from all sources exhibited significantly higher sodium cholate and cholesterol-binding capacity compared to cellulose, and also retarded pancreatic cholesterol esterase activity in a concentration-dependent manner. Conclusion: This study demonstrates that natural dietary fiber from salad vegetables can reduce glucose and lipid absorption and breakdown rates, thus preventing increases in postprandial blood glucose and cholesterol levels, which can be beneficial to human health.

Asian Journal of Pharmaceutical and Clinical Research, Apr 1, 2017

Objective: The objectives of this study were to isolate microorganisms and screen for potential a... more Objective: The objectives of this study were to isolate microorganisms and screen for potential antimicrobial activities from the soil. Methods: In this study, a total of 425 isolates were isolated from 100 soil samples. The preliminary screening for antimicrobial activities of these isolates was performed by modified cross-streak, agar diffusion, and modified microdilution technique against 16 pathogenic bacteria and fungi. Results: In the anti-microbial activity, there were three isolates, namely, 277, 303, and 307 exhibited inhibitory activity against methicillin-resistant Staphylococcus aureus and Salmonella typhimurium respectively. This study also examined the various enzymes producing from soil microorganisms including chitinase, chitosanase, amylase, cellulose, caseinase, gelatinase, esterase, and lipase production of different selective media for 24 and 48 hrs using the direct spot method. The results revealed that 28 isolates could produce various enzymes with strong activity. Most of them produced gelatinase (5.65%) and caseinase (5.18%). There were four isolates that produce broad-spectrum enzyme. In addition, the investigation of selected microorganism identification showed that they can be divided into three groups: Burkholderia spp., Pseudomonas spp., and Rhodococcus spp. Conclusion: This study demonstrated that the microorganisms from soil are capable of producing potential, antibacterial, and bioactive enzymes.

Journal of Food Science and Technology, Feb 5, 2020

Prebiotics are functional foods with health-promoting properties that are used in many developed ... more Prebiotics are functional foods with health-promoting properties that are used in many developed countries. Thailand is one of the countries that produces many plants that should have prebiotic properties. In this study, we investigated the potential prebiotic effects of powders obtained from Saba, Pisang Awak Banana and Silver bluggoe in vitro in accordance with their physical, chemical and microbiological properties. These selected plants were found to demonstrate good water-/oil-binding properties. They contained chlorophyll, beta carotene and lycopene and showed good resistance to stomach and small-intestine enzymes. The selected plants were further used to evaluate prebiotic properties by supplementing as a carbon source in culturing broth for growing probiotic bacteria and pathogenetic bacteria. The increase in the number of probiotic bacteria during fermentation of these selected plants correlated with decreased pH. The growth of four strains of probiotic bacteria seemed to be promoted in MRS broth containing these selected plants, but no significant differences in the number of probiotic bacterial groups were detected in response to difference concentrations of all these selected plants. In addition, we noted that a decrease in the number of all four strains of pathogenic bacteria during fermentation of these selected plants correlated with a decreased pH. Moreover, the antimicrobial activity of selected plant prebiotics supported probiotic substance production to inhibit growth of pathogenic bacteria. In conclusion, we have shown that the addition of selected prebiotic plants, indicating that they should be used as a prebiotic food ingredient, represents a potential alternative to available commercial prebiotics.

Pakistan Journal of Nutrition, Jan 15, 2015

Asian Journal of Pharmaceutical and Clinical Research, Sep 7, 2018

Objectives: The objectives of this study were to investigate the in vitro biological effect of th... more Objectives: The objectives of this study were to investigate the in vitro biological effect of the methanolic crude extract received from edible mushrooms; Flammulina velutipes on anti-inflammatory, antioxidant activity, anti-lipid peroxidation (LPO), antimicrobial activity, and anticancer activity. The scientific result obtained here was possibility of developing this variety as an ingredient in health/medical food purposes as well as a functional food. Methods: In this study, the methanol crude extract of F. velutipes has been performed and in vitro pharmacological properties such as anti-inflammatory, antioxidant activity, anti-LPO, antimicrobial activity, and anticancer activity were evaluated. Results: The results revealed that anti-inflammatory activity of crude extract was very significant with 79.81±0.87% of the dose-dependent manner. Antioxidant and anti-low-density lipoprotein (LDL) peroxidation activity of the extract showed its capacity of free radical scavenging with highlevel antioxidant compounds and lipid preventive effects (2, 2-diphenyl-1-picrylhydrazyl: 79.81±0.01%, total phenol: 991.10±0.01 µg gallic acid equivalents/0.1 g dry matter, H 2 O 2: 93.27±0.05%, LPO: 33.33±0.00%, and LDL: 57±0.03%, respectively). Analysis has also demonstrated that the methanolic extract of F. velutipes provided an antimicrobial activity to ESBL-producing Klebsiella pneumoniae. Moreover, F. velutipes extract showed a growth inhibitory effect in HepG2 cells with the 50% inhibitory concentration (IC 50) value IC 50 =8.25±7.59 µg/ml. Conclusion: This indicated that crude extract from F. velutipes was scientifically proved as a potential source of therapeutic agent and be able to utilize as a functional ingredient for healthy or supplement food.

Journal of medical pharmaceutical and allied sciences, Dec 31, 2022

Antimicrobial activity and safety applications of meso-tetra(4-pyridyl)platinum(II) porphyrin

Journal of Medical Society, 2022

International Journal of Phytomedicine, Jun 13, 2013

A total of 69 endophytic fungi were isolated from Sesbania grandiflora (L.) Pers. Their antimicro... more A total of 69 endophytic fungi were isolated from Sesbania grandiflora (L.) Pers. Their antimicrobial activities were already primary screened by agar diffusion assay and antagonistic activities. The ethyl acetate crude extracts of 28 selected endophytic fungi were further determined for Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) or Minimum Fungicidal Concentration (MFC) against bacteria (Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 278530), yeast (Candida albicans and Cryptococcus neoformans) and mold (Rhizopus spp., Mucor spp., Aspergillus spp., Alternaria spp., Curvularia spp., and Penicillium spp.) by using the standard broth microdilution method and a modified resazurin microtiter plate assay. It was found that 26 in 28 extracts exhibited high anti bacterial activity against B. subtilis with the susceptibility rate at 92.86% than P.aeruginosa (82.14%), S.aureus (42.86%), and E.coli (7.14%) respectively. The MIC and MBC of this group were in the range of 0.49-250µg/mL and 1.95≥250µg/mL, respectively. Seventeen extracts (60.71%) showed antifungal activity against C.neoformans than C.albicans (42.86%) with the MIC and MFC were in the range of 31.25-250µg/mL and >250µg/mL, respectively, whereas 23 extracts (82.14%) showed high antifungal activity against Mucor spp. than Rhizopus spp. (75%), Alternaria spp. (60.71%), Curvularia spp.(57.14%), Penicillium spp. (53.57%) and Aspergillus spp. (3.57%) respectively, with MIC and MFC in the range of 62.50-250µg/mL and >250µg/mL respectively. Study of macroscopic and microscopic examination of fungal morphology revealed that most of endophytic fungi are hyaline septate hyphae but 3 isolates are hyaline non-septate hyphae. Fusarium spp. and Acremonium spp. are the predominate species among selected endophytic fungi. These results of this study strongly suggested that metabolites of endophytic fungi from Sesbania grandiflora (L.) Pers. could be a good potential source of bioactive natural products against some bacteria yeast, and molds.

International Journal of Pharmacy and Pharmaceutical Sciences, Feb 3, 2017

Pectinases are industrially important enzymes. Bacillus spp. isolated from soil sample was used f... more Pectinases are industrially important enzymes. Bacillus spp. isolated from soil sample was used for pectinase production utilizing orange peels as its substrate under submerged fermentation. Enzyme activity Increased with increase in temperature with peak at 30 °C which decreased with further increase in temperature. At pH 7, enzyme activity was optimum which decreased with increase in alkalinity, temperature and incubation time. Maximum activity of the pectinase enzyme was obtained with glucose at pH7 and temperature of 30°C. Supplementary carbon sources viz;-glucose, sucrose , lactose and orange peel were crucial for pectinase activity demonstration at 30°C. Glucose enhanced the highest pectinase activity of 54.92 U/ml. Bacillus spp. demonstrated high pectinase enzyme activity, which is an indication that this organism is of great importance for industrial pectinase enzyme activity demonstration. It is expected that with further strain improvement and substrate modification, greater activity can be achieved.

Plasma medicine, 2016

Atmospheric Dielectric Barrier Discharge (DBD) plasma development is one of the most interesting ... more Atmospheric Dielectric Barrier Discharge (DBD) plasma development is one of the most interesting subjects to our group. Fridman et al. * has concluded that Floating DBD Direct Plasma are more effective in bactericidal property, it contains higher free charged particles than indirect type. DBD plasma sources can provide low temperature or non-thermal (cold) plasmas, and these plasmas can be created with ambient air or with a noble gas, such as helium or argon, as the carrier medium. It can be used with an electrode as a contact or noncontact method to target a tissue. However, the drawback of direct DBD plasma is that it will work only in short gap distances, on the order of a few millimeters. Our group has investigated-PLASMA Cell Modulation: PhotoBioCare, Thailand). The major area has been focused on its bactericidal disinfection property, application in common dermatologic problems, in skin aesthetic facial rejuvenation and chronic wound management, from 2012 to 2016.

Journal of Health Science, 2020

The modern pharmaceutical manufacturing industry in Thailand remains a downstream industry. In 20... more The modern pharmaceutical manufacturing industry in Thailand remains a downstream industry. In 2019, there were 142 GMP certified modern pharmaceutical manufacturing facilities, most of which are manufacturers of finished products containing pharmaceutical chemicals. The manufacturing industry in 2018 was worth 77 billion baht, an increase of 5% from the previous year with a net profit of about 8 %. Although the export of pharmaceutical products has increased, competition is high and the competitive advantages have decreased compared to the potential of import trade. In 2015, the ratio of production value to import value was approximately 35:65. Businesses were more interested in research and development, mostly focusing on the development of generic drugs and new generic drugs in conventional dosage forms. The motivation for production came from the Thai Innovation List policy. Since 2009, documents for drug registration application must comply with the format specified by ASEAN. I...

International Journal of Medical Toxicology & Legal Medicine, 2021

Journal of Medical Society

Pakistan Journal of Nutrition, 2015