Pantaleo Scelza - Academia.edu (original) (raw)

Papers by Pantaleo Scelza

O objetivo do presente trabalho foi avaliar e comparar o efeito antimicrobiano do MTA Dentsply, M... more O objetivo do presente trabalho foi avaliar e comparar o efeito antimicrobiano do MTA Dentsply, MTA Angelus, hidróxido de cálcio e cimento Portland sobre quatro cepas bacterianas: Pseudomonas aeruginosa, Escherichia coli, Bacteroides fragilis, e Enterococcus faecalis. Placas contendo agar Muller-Hinton suplementadas com 5% de sangue de carneiro, hemina e menadiona foram inoculadas com as suspensões bacterianas. Poços foram confeccionados com auxílio de perfuradores e imediatamente preenchidos com os materiais, e incubados a 37ºC por 48 horas em atmosfera de anaerobiose, exceto P. aeruginosa. O diâmetro dos halos de inibição foi medido e os dados analisados usando o teste estatístico ANOVA e o de Tukey com nível de significância de 1%. O MTA Dentsply, MTA Angelus e Cimento Portland inibiram o crescimento da P.aeruginosa. O hidróxido de cálcio foi efetivo contra P. aeruginosa e B. fragillis. Sob atmosfera de anaerobiose, condição que pode impedir a formação de espécies reativas do oxi...

Research, Society and Development, 2021

In cases of injuries in the oral cavity, the mouthwash comes in contact with the underlying gingi... more In cases of injuries in the oral cavity, the mouthwash comes in contact with the underlying gingival connective tissue and should have its cytotoxicity assessed. However, there is no available evidence if cells of elderly donors react differently during in vitro assessments of mouthwashes. This study aimed to compare the cytotoxicity evaluation of two different mouthwash types when assessed with primary gingival fibroblasts from either young and older donors. Primary cells were collected from two elderly patients (mean age 66.5 years old) and two young patients (mean age 27.5 years old). The primary cell culture was produced from gingival fragments and exposed for 24h in Perioxidin® and Oral B®. A control group was exposed to unconditioned culture media, representing 100% of cell survival (negative control), and 200mg/mL solution of latex fragments was used as a positive control due to its well-known toxicity. Both products presented similar dose-dependent cytotoxicity. In the toxi...

The Journal of Contemporary Dental Practice, 2021

Aim: This systematic review aimed to establish the available techniques for fiber post removal, p... more Aim: This systematic review aimed to establish the available techniques for fiber post removal, pointing out where each method stands out evaluating its advantages, and where they fall short indicating the possible harmful effects. Materials and methods: This systematic review was registered in PROSPERO (CRD42020193799). A broad search of the electronic databases PubMed, Web of Science, Scopus, and Virtual Health Library was carried out before April 27, 2020, for in vitro studies about techniques for removing fiber posts luted with resin cements of endodontically treated teeth. The methodological quality of included studies was evaluated based on the critical assessment tool for in vitro studies. Results: Thirteen articles were selected and included after applying the inclusion and exclusion criteria. All included studies received an assessment of methodological quality between high and moderate. Most studies used ultrasonic inserts as a strategy to remove fiber posts, manufactured removal kits and drills were also widely used, only one study evaluated the removal capacity of the Er:YAG laser. Ultrasonic inserts seem to lead to a greater working time for removal of the fiber post, tend to generate significant volume changes in the root structure and increase the temperature on the root canal and clean the root canal walls further. Conclusion: Although the results tend to show greater agility in removing fiber posts with manufactured removal kits and the ultrasonic inserts seem to work better in removing fiber remains and luting agent, there is still no consensus in the literature as to which technique is the best. Clinical Significance: In some cases, clinicians may be faced with the need to remove fiber posts in order to regain access to the root canal due to the need for endodontic retreatment which directly implies the search for the most appropriate removal technique.

iranian endodontic journal, 2020

Introduction: Irregularities and defects on NiTi endodontic instruments originating from the manu... more Introduction: Irregularities and defects on NiTi endodontic instruments originating from the manufacturing process can lead to the structural collapse and fracture of these instruments during treatment. To assess the cause of instrument wear and fracture, as well as increasing fracture incidence, destructive and non-destructive methods have been used for the analysis of surfaces and internal structures of new and used NiTi instruments. The aim of this systematic review was to undertake a detailed analysis of the methods used to evaluate the surface and internal microstructure of endodontic instruments. Methods and Materials: The scientific literature was comprehensively and systematically searched in the MEDLINE (PubMed), Web of Science, Cochrane Library, Scopus, and LILACS/BBO databases for studies published up to June 9, 2019. The eligibility criteria was based on the PICO (Patient, Intervention, Comparison, and Outcome) strategy with the question “What is the best method for stru...

Materials, 2021

This work aimed to investigate the use of Regenerative Endodontic Procedures (REP) on the treatme... more This work aimed to investigate the use of Regenerative Endodontic Procedures (REP) on the treatment of pulp necrosis in mature teeth through systematic review and meta-analysis of evidence on clinical and radiographic parameters before and after REP. A search was performed in different databases on 9 September 2020, including seven clinical studies and randomized controlled trials (RCT). The methodological quality was assessed using Revised Cochrane risk-of-bias (RoB 2) and Before-and-After tools. Meta-analyses were performed to evaluate the success incidences regarding the reduction of periapical lesion and recovery of sensitivity. The certainty of the evidence was assessed using GRADE. Meta-analysis showed a high overall success of 0.95 (0.92, 0.98) I2 = 6%, with high periapical lesion reduction at 12 months (0.93 (0.86, 0.96) I2 = 37%) and by the end of follow-up (0.91 (0.83, 0.96) I2 = 13%). Lower incidences of positive sensitivity response were identified for the electrical (0....

Materials, 2021

Irrigant solutions are used to promote dentin-growth factors (GF) release for regenerative endodo... more Irrigant solutions are used to promote dentin-growth factors (GF) release for regenerative endodontics. This review aimed to evaluate the reports comparing the release of GFs using different root canal irrigant solutions. Eligible studies compared the in vitro GF release in human teeth after the use of at least two distinct solutions. A search was conducted on Pubmed, Scopus, Web of Science, and Lilacs on 11 August 2021. Risk of bias was assessed using SciRAP. Study characteristics and quantitative data were extracted, and meta-analyses were performed for the mean difference (95% confidence interval) of the release of transforming growth factors Beta 1 (TGF-β1) by EDTA compared to other irrigants. Of sixteen eligible studies, eight were included in quantitative analysis. ELISA assays showed higher TGF-β1 release from 10% EDTA compared to 10% citric acid (p < 0.00001). Immunogold assays showed higher levels of TGF-β1 for 17% EDTA (p < 0.00001) compared to 10% citric acid. GRADE...

Archives of Oral Biology, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Revista Brasileira de Geriatria e Gerontologia, 2019

Objectives : To evaluate the functional and physiological structures of the stomatognathic system... more Objectives : To evaluate the functional and physiological structures of the stomatognathic system of the oral cavity of older adults based on self-perception, comparing the same with a professional clinical evaluation, and investigating the difficulties encountered when chewing. Method : An analytical cross-sectional study with a quantitative approach was conducted with a sample of 53 older adults aged 60 to 90 years. A protocol consisting of three questionnaires was used: a sociodemographic evaluation, a self-perception based interview with 19 questions on the chewing of the older adults and a clinical evaluation containing 30 questions covering aspects of the oral cavity tissue. The self-perception and clinical evaluation scores were compared using the Mann-Whitney test and the proportions observed for each item were compared by the binomial test. Results : It was found that the self-perception of older adults did not correspond to the result of the clinical evaluation. While 31 (...

The Journal of Contemporary Dental Practice, 2016

Aim The aim of this study was to evaluate the inflammatory response to MTA Fillapex, AH Plus, and... more Aim The aim of this study was to evaluate the inflammatory response to MTA Fillapex, AH Plus, and Pulp Canal Sealer Extensive Work Time (EWT), in a murine bone defect grafting model. Materials and methods Bilateral mandibular critical defects were produced in 45 Wistar rats with a trephine bur#2 and filled with the endodontic sealers. After 7, 14, and 28 days, the rats were euthanized and their jaws were histologically prepared. Results For the 7-day group, no statistical significance was observed among all studied groups (p > 0.05), and high levels of inflammatory infiltrate were detected. After 14 and 28 days, Pulp Canal Sealer EWT showed statistically lower inflammatory response in comparison to other sealers (p < 0.05) except for the control group (no sealers). Conclusion Pulp Canal Sealer EWT presented the lowest levels of inflammatory response. The critical defect grafting model was an effective method to detect differences among differences on the biological response to...

Engineering Failure Analysis, 2019

The aim of this study was to investigate the methodological potential of a new application of mic... more The aim of this study was to investigate the methodological potential of a new application of micro-computed tomography in providing an external and internal assessment of the structural integrity of an endodontic test file, and its progressive structural deterioration after repeated use in simulated root canals (resin blocks), until fracture. The progressive deterioration of the instrument was observed, and the external and internal defects were described. Radiolucent images suggestive of micro-cavities were observed in the instrument's core prior to its first use, and the formation and subsequent propagation of cracks in an apparently non-linear manner were observed after consecutive uses, until the final collapse of the whole structure. This novel assessment method allowed the visualization the external surface and the core of the same instrument at different time points in the instrumentation process, thus enabling researchers to identify where the cracks started and their progression during the course of instrument use.

Brazilian Dental Journal, 2018

In order to evaluate the volume of dentinal cracks taking into account the age of the dentin and ... more In order to evaluate the volume of dentinal cracks taking into account the age of the dentin and the type of file system used for endodontic procedures, forty freshly extracted single-rooted lower incisive teeth presenting similar root volume, were divided into two groups according to the age of the patient: Group Young (18 - 30 years old) and Group Old (60 years old or more). Each specimen was scanned by microcomputed tomography (micro-CT) in three stages: (i) before any treatment, (ii) after endodontic treatment with Reciproc files (REC), and (iii) after subsequent endodontic retreatment. Each group was subdivided into two subgroups, according to the retreatment technique used: retreatment with REC or with ProTaper Universal Retreatment (PUR) files. For each subgroup, the images were analyzed for differences in the volume of dentinal cracks in the middle and apical thirds of the teeth , according to the dentin age. In both stages (before and after instrumentation), the micro-CT im...

Dental Materials, 2018

Objectives. The increased demand for esthetics and minimally invasive tooth restorations resulted... more Objectives. The increased demand for esthetics and minimally invasive tooth restorations resulted in a rapid development of adhesive dentistry. However, much controversy remains about the safe use and cytotoxic effects of different groups of dental adhesives. The present study performed a systematic review to identify the answer to the following question: are self-etch adhesives more cytotoxic than those employing the etch-and-rinse system? Methods. This systematic review was performed in accordance with the PRISMA statement; a quality assessment for in vitro studies was conducted using the ToxRTool. Specific search strategies were developed and performed in the electronic databases MEDLINE via PubMed, Cochrane Library, Scopus, Web of Science, and LILACS/BBO. After removal of duplicated studies and application of the exclusion criteria, ten eligible articles were selected and submitted to a qualitative descriptive analysis comparing both groups of dental adhesives. Most in vitro test systems employed pulp cells or gingival fibroblasts. Results. The methodologies presented great variability regarding the exposure to the test materials. Only four studies assessed the role of the degree of conversion of the materials in their toxicity, with conflicting results. Significance. While the lack of methodological standardization among the studies still hinders the establishment of a relationship between type of dental adhesive and toxicity, studies employing dentin barrier systems indicate greater cytotoxicity for etch-and-rinse adhesives.

Iranian endodontic journal, 2018

This study evaluated the removal of the filling material during endodontic retreatment considerin... more This study evaluated the removal of the filling material during endodontic retreatment considering the presence of cracks and the dentin age. A total of 20 freshly extracted single-rooted teeth were categorized into the following two groups according to the age of the patients: Group Young (Y; aged 18-30 years) and Group Old (O; aged ≥60 years). Each tooth specimen was scanned by microcomputed tomography (micro-CT) subsequently after endodontic retreatment with the Reciproc instruments (REC). The images were analyzed for differences in the volume of dentin cracks and the presence of the filling material in the middle and apical thirds of the teeth among the groups, according to the dentin age. The micro-CT images showed that after retreatment, there were more cracks in the old root dentin than those in the young root dentin, although the difference was not statistically significant (>0.05). The greatest reduction in the filling material was achieved when the old root dentin with ...

Journal of applied oral science : revista FOB, 2018

Denture adhesives (DA) improve the retention and stability of ill-fitting dentures, especially fo... more Denture adhesives (DA) improve the retention and stability of ill-fitting dentures, especially for older adults. These materials should be biocompatible, i.e., they cannot cause undesired biological responses and be non-cytotoxic to oral tissues. However, in vitro testing of DA biocompatibility employing primary cell culture may possibly be affected by other factors, such as the donor age. To compare the cytotoxicity of three different denture adhesives when assessed in primary gingival fibroblasts from a young donor or from an older donor, as well as the release of the basic fibroblast growth factor (bFGF), and the inflammatory response marker interleukin-6 (IL-6). Gingival fibroblasts isolated from a 30- and a 62-year-old donor were assayed for proliferation (1-7 days) and sensitivity to latex (positive control). Fibroblasts were indirectly exposed to Corega Ultra (cream), Corega powder and Fixodent Original for a 24 h period and assayed by XTT and Crystal Violet tests. The releas...

Brazilian oral research, Jan 28, 2017

The aim of this study was to undertake a qualitative and quantitative assessment of nanoscale alt... more The aim of this study was to undertake a qualitative and quantitative assessment of nanoscale alterations and wear on the surfaces of nickel-titanium (NiTi) endodontic instruments, before and after use, through a high-resolution, noncontact, three-dimensional optical profiler, and to verify the accuracy of the evaluation method. Cutting blade surfaces of two different brands of NiTi endodontic instruments, Reciproc R25 (n = 5) and WaveOne Primary (n = 5), were examined and compared before and after two uses in simulated root canals made in clear resin blocks. The analyses were performed on three-dimensional images which were obtained from surface areas measuring 211 × 211 µm, located 3 mm from their tips. The quantitative evaluation of the samples was conducted before and after the first and second usage, by the recordings of three amplitude parameters. The data were subjected to statistical analysis at a 5% level of significance. The results revealed statistically significant incre...

Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 2017

Objective: To compare the bond strength of three-step and self-etch adhesive systems with and wit... more Objective: To compare the bond strength of three-step and self-etch adhesive systems with and without prior acid etching on bonding of fiberglass posts in root canals. Material and Methods: Experiments were conducted on roots of five upper molars without curvature and sectioned in a cervical-apical direction. With the aid of a highspeed turbine and diamond tips, cylindrical cavities 7 mm in depth and 1.4 mm in diameter were made in the dentine. The roots of the same tooth were used, where the retainers were cemented, thus generating three groups: Group A: Adper Scotchbond Multipurpose Plus (SBMP) + Rely X ARC; Group B: Single Bond Universal (SBU-1) + Rely X ARC, with acid etching prior to adhesive insertion; Group C: Single Bond Universal (SBU-2) + Rely X ARC. The samples were stored in a bacteriological incubator at 37ºC with 100% humidity for 48h and were subsequently cross-sectioned to obtain 3 dentine discs 1mm in thickness. Forty-five specimens were subjected to the push-out test. Results: Through the analysis of variance and Tukey's test, it was found that SBMP showed, with statistical significance (p<0.05), highest bond strength when compared with the SBU-1 and SBU-2 systems. The observed difference in the discs was located in the cervical portion. Conclusion: The three-step adhesive system had higher bond strength with the substrate than the self-etching adhesive only in the disc in the cervical portion; further, statistically, the prior application of phosphoric acid in SBU-1 did not affect its bond strength significantly.

Brazilian dental journal

Bis-acryl resins are used for temporary dental restorations and have shown advantages over other ... more Bis-acryl resins are used for temporary dental restorations and have shown advantages over other materials. The aim of this work was to evaluate the in vitro cytotoxicity of two bis-acryl composite resins (Protemp 4 and Luxatemp Star), obtained at 1, 7 and 40 days after mixing the resin components, using a standardized assay employing human primary cells closely related to oral tissues. Human gingival fibroblast cell cultures were exposed for 24 h to either bis-acryl composite resins, polystyrene beads (negative control) and latex (positive control) extracts obtained after incubation by the different periods, at 37 °C under 5% CO2. Cell viability was evaluated using a multiparametric procedure involving sequential assessment (using the same cells) of mitochondrial activity (XTT assay), membrane integrity (neutral red test) and total cell density (crystal violet dye exclusion test). The cells exposed to the resin extracts showed cell viability indexes exceeding 75% after 24 h. Even w...

Iranian endodontic journal, 2016

The aim of this in vitro study was to evaluate the effect of 10% citric acid and 17% ethylenediam... more The aim of this in vitro study was to evaluate the effect of 10% citric acid and 17% ethylenediaminetetraacetic acid (EDTA) irrigating solutions on the surface morphology of young and old root dentin by determining the number and diameter of dentinal tubules using scanning electron microscopy (SEM). Fifty healthy human teeth collected from young (≤30 years) and old (≥60 years) individuals (n=25) were first prepared with a Largo bur #2 to produce smear layer on the root canal surface. Subsequently, the crowns and the root middle and apical thirds were sectioned and removed, and the cervical thirds were sectioned vertically in the buccal-lingual direction into two equal halves. The obtained samples were then immersed in 2.5% sodium hypochlorite for 30 min and randomly separated into two treatment groups for each age group. In each age group, ten samples were selected as controls and did not receive any type of treatment. The rest of the specimens were then rinsed, dried and treated fo...

The aim of this study was to evaluate comparatively the physical properties of solubility, disint... more The aim of this study was to evaluate comparatively the physical properties of solubility, disintegration and flow of some root canal sealers: SEALAPEX™, PULP CANAL SEALER™, TUBLISEAL™, AH PLUS™, AH 26™, TOP SEAL™, SEALER PLUS™, SEALER 26™ and ENDOFILL™. Method: Methodology used was according ISO 6786 which test the physicochemical properties using five proof specimens for each test. Results: In terms of solubility, the root canal sealers analyzed have been classified in the following decreasing order: ENDOFILL™, SEALAPEX™, PULP CANAL SEALER™, AH 26™, TUBLISEAL™, SEALER PLUS™, AH PLUS™, TOP SEAL™ and SEALER 26™, where a statistically significant difference was found at a 1% level. Between TOP SEAL™ and SEALER™ 26 root canal sealers there was no statistically significant difference. None of the sealers above have shown disintegration. Regarding flow, the root canal sealers in the study have been classified in the following decreasing order: TUBLISEAL™ root canal sealer, followed by ENDOFILL™, SEALAPEX™, PULP CANAL SEALER™, AH PLUS™, TOP SEAL™, AH 26™, SEALER 26™ and SEALER PLUS™, where a statistically significant difference was found at a 1% level. Conclusion: The solubility, only the SEALAPEX™ and ENDOFILL™ have shown a limit above determined by ISO 6786.

Brazilian Oral Research, 2015

The aim of this study was to comparatively evaluate the bending resistance at 45º, the static and... more The aim of this study was to comparatively evaluate the bending resistance at 45º, the static and dynamic cyclic fatigue life, and the fracture type of the WaveOne (Dentsply Maillefer, Ballaigues, Switzerland) 25-08 and Reciproc (VDW, Munich, Germany) 25-08 instruments. A total of 60 nickel-titanium (NiTi) instruments (30 Reciproc and 30 WaveOne) from three different lots, each of which was 25 mm in length, were tested. The bending resistance was evaluated through the results of a cantilever-bending test conducted using a universal testing machine. Static and dynamic cyclic fatigue testing was conducted using a custom-made device. For the static and dynamic tests, a cast Ni-Cr-Mo-Ti alloy metal block with an artificial canal measuring 1.77 mm in diameter and 20.00 mm in total length was used. A scanning electron microscope was used to determine the type of fracture. Statistical analyses were performed on the results. The WaveOne instrument was less flexible than the Reciproc (p < 0.05). The Reciproc instrument showed better resistance in the static and dynamic cyclic fatigue tests (p < 0.05). The transverse cross-section and geometry of the instruments were important factors in their resistance to bending and cyclic fracture. Both of the instruments showed ductile-type fracture characteristics. It can be concluded that the Reciproc 25-08 instrument was more resistant to static and dynamic cyclic fatigue than the WaveOne 25-08 instrument, while the WaveOne 25-08 instrument was less flexible. Bending and resistance to cyclic fracture were influenced by the instruments' geometries and transverse cross-sections. Both of the instruments showed ductile-type fracture characteristics.

O objetivo do presente trabalho foi avaliar e comparar o efeito antimicrobiano do MTA Dentsply, M... more O objetivo do presente trabalho foi avaliar e comparar o efeito antimicrobiano do MTA Dentsply, MTA Angelus, hidróxido de cálcio e cimento Portland sobre quatro cepas bacterianas: Pseudomonas aeruginosa, Escherichia coli, Bacteroides fragilis, e Enterococcus faecalis. Placas contendo agar Muller-Hinton suplementadas com 5% de sangue de carneiro, hemina e menadiona foram inoculadas com as suspensões bacterianas. Poços foram confeccionados com auxílio de perfuradores e imediatamente preenchidos com os materiais, e incubados a 37ºC por 48 horas em atmosfera de anaerobiose, exceto P. aeruginosa. O diâmetro dos halos de inibição foi medido e os dados analisados usando o teste estatístico ANOVA e o de Tukey com nível de significância de 1%. O MTA Dentsply, MTA Angelus e Cimento Portland inibiram o crescimento da P.aeruginosa. O hidróxido de cálcio foi efetivo contra P. aeruginosa e B. fragillis. Sob atmosfera de anaerobiose, condição que pode impedir a formação de espécies reativas do oxi...

Research, Society and Development, 2021

In cases of injuries in the oral cavity, the mouthwash comes in contact with the underlying gingi... more In cases of injuries in the oral cavity, the mouthwash comes in contact with the underlying gingival connective tissue and should have its cytotoxicity assessed. However, there is no available evidence if cells of elderly donors react differently during in vitro assessments of mouthwashes. This study aimed to compare the cytotoxicity evaluation of two different mouthwash types when assessed with primary gingival fibroblasts from either young and older donors. Primary cells were collected from two elderly patients (mean age 66.5 years old) and two young patients (mean age 27.5 years old). The primary cell culture was produced from gingival fragments and exposed for 24h in Perioxidin® and Oral B®. A control group was exposed to unconditioned culture media, representing 100% of cell survival (negative control), and 200mg/mL solution of latex fragments was used as a positive control due to its well-known toxicity. Both products presented similar dose-dependent cytotoxicity. In the toxi...

The Journal of Contemporary Dental Practice, 2021

Aim: This systematic review aimed to establish the available techniques for fiber post removal, p... more Aim: This systematic review aimed to establish the available techniques for fiber post removal, pointing out where each method stands out evaluating its advantages, and where they fall short indicating the possible harmful effects. Materials and methods: This systematic review was registered in PROSPERO (CRD42020193799). A broad search of the electronic databases PubMed, Web of Science, Scopus, and Virtual Health Library was carried out before April 27, 2020, for in vitro studies about techniques for removing fiber posts luted with resin cements of endodontically treated teeth. The methodological quality of included studies was evaluated based on the critical assessment tool for in vitro studies. Results: Thirteen articles were selected and included after applying the inclusion and exclusion criteria. All included studies received an assessment of methodological quality between high and moderate. Most studies used ultrasonic inserts as a strategy to remove fiber posts, manufactured removal kits and drills were also widely used, only one study evaluated the removal capacity of the Er:YAG laser. Ultrasonic inserts seem to lead to a greater working time for removal of the fiber post, tend to generate significant volume changes in the root structure and increase the temperature on the root canal and clean the root canal walls further. Conclusion: Although the results tend to show greater agility in removing fiber posts with manufactured removal kits and the ultrasonic inserts seem to work better in removing fiber remains and luting agent, there is still no consensus in the literature as to which technique is the best. Clinical Significance: In some cases, clinicians may be faced with the need to remove fiber posts in order to regain access to the root canal due to the need for endodontic retreatment which directly implies the search for the most appropriate removal technique.

iranian endodontic journal, 2020

Introduction: Irregularities and defects on NiTi endodontic instruments originating from the manu... more Introduction: Irregularities and defects on NiTi endodontic instruments originating from the manufacturing process can lead to the structural collapse and fracture of these instruments during treatment. To assess the cause of instrument wear and fracture, as well as increasing fracture incidence, destructive and non-destructive methods have been used for the analysis of surfaces and internal structures of new and used NiTi instruments. The aim of this systematic review was to undertake a detailed analysis of the methods used to evaluate the surface and internal microstructure of endodontic instruments. Methods and Materials: The scientific literature was comprehensively and systematically searched in the MEDLINE (PubMed), Web of Science, Cochrane Library, Scopus, and LILACS/BBO databases for studies published up to June 9, 2019. The eligibility criteria was based on the PICO (Patient, Intervention, Comparison, and Outcome) strategy with the question “What is the best method for stru...

Materials, 2021

This work aimed to investigate the use of Regenerative Endodontic Procedures (REP) on the treatme... more This work aimed to investigate the use of Regenerative Endodontic Procedures (REP) on the treatment of pulp necrosis in mature teeth through systematic review and meta-analysis of evidence on clinical and radiographic parameters before and after REP. A search was performed in different databases on 9 September 2020, including seven clinical studies and randomized controlled trials (RCT). The methodological quality was assessed using Revised Cochrane risk-of-bias (RoB 2) and Before-and-After tools. Meta-analyses were performed to evaluate the success incidences regarding the reduction of periapical lesion and recovery of sensitivity. The certainty of the evidence was assessed using GRADE. Meta-analysis showed a high overall success of 0.95 (0.92, 0.98) I2 = 6%, with high periapical lesion reduction at 12 months (0.93 (0.86, 0.96) I2 = 37%) and by the end of follow-up (0.91 (0.83, 0.96) I2 = 13%). Lower incidences of positive sensitivity response were identified for the electrical (0....

Materials, 2021

Irrigant solutions are used to promote dentin-growth factors (GF) release for regenerative endodo... more Irrigant solutions are used to promote dentin-growth factors (GF) release for regenerative endodontics. This review aimed to evaluate the reports comparing the release of GFs using different root canal irrigant solutions. Eligible studies compared the in vitro GF release in human teeth after the use of at least two distinct solutions. A search was conducted on Pubmed, Scopus, Web of Science, and Lilacs on 11 August 2021. Risk of bias was assessed using SciRAP. Study characteristics and quantitative data were extracted, and meta-analyses were performed for the mean difference (95% confidence interval) of the release of transforming growth factors Beta 1 (TGF-β1) by EDTA compared to other irrigants. Of sixteen eligible studies, eight were included in quantitative analysis. ELISA assays showed higher TGF-β1 release from 10% EDTA compared to 10% citric acid (p < 0.00001). Immunogold assays showed higher levels of TGF-β1 for 17% EDTA (p < 0.00001) compared to 10% citric acid. GRADE...

Archives of Oral Biology, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Revista Brasileira de Geriatria e Gerontologia, 2019

Objectives : To evaluate the functional and physiological structures of the stomatognathic system... more Objectives : To evaluate the functional and physiological structures of the stomatognathic system of the oral cavity of older adults based on self-perception, comparing the same with a professional clinical evaluation, and investigating the difficulties encountered when chewing. Method : An analytical cross-sectional study with a quantitative approach was conducted with a sample of 53 older adults aged 60 to 90 years. A protocol consisting of three questionnaires was used: a sociodemographic evaluation, a self-perception based interview with 19 questions on the chewing of the older adults and a clinical evaluation containing 30 questions covering aspects of the oral cavity tissue. The self-perception and clinical evaluation scores were compared using the Mann-Whitney test and the proportions observed for each item were compared by the binomial test. Results : It was found that the self-perception of older adults did not correspond to the result of the clinical evaluation. While 31 (...

The Journal of Contemporary Dental Practice, 2016

Aim The aim of this study was to evaluate the inflammatory response to MTA Fillapex, AH Plus, and... more Aim The aim of this study was to evaluate the inflammatory response to MTA Fillapex, AH Plus, and Pulp Canal Sealer Extensive Work Time (EWT), in a murine bone defect grafting model. Materials and methods Bilateral mandibular critical defects were produced in 45 Wistar rats with a trephine bur#2 and filled with the endodontic sealers. After 7, 14, and 28 days, the rats were euthanized and their jaws were histologically prepared. Results For the 7-day group, no statistical significance was observed among all studied groups (p > 0.05), and high levels of inflammatory infiltrate were detected. After 14 and 28 days, Pulp Canal Sealer EWT showed statistically lower inflammatory response in comparison to other sealers (p < 0.05) except for the control group (no sealers). Conclusion Pulp Canal Sealer EWT presented the lowest levels of inflammatory response. The critical defect grafting model was an effective method to detect differences among differences on the biological response to...

Engineering Failure Analysis, 2019

The aim of this study was to investigate the methodological potential of a new application of mic... more The aim of this study was to investigate the methodological potential of a new application of micro-computed tomography in providing an external and internal assessment of the structural integrity of an endodontic test file, and its progressive structural deterioration after repeated use in simulated root canals (resin blocks), until fracture. The progressive deterioration of the instrument was observed, and the external and internal defects were described. Radiolucent images suggestive of micro-cavities were observed in the instrument's core prior to its first use, and the formation and subsequent propagation of cracks in an apparently non-linear manner were observed after consecutive uses, until the final collapse of the whole structure. This novel assessment method allowed the visualization the external surface and the core of the same instrument at different time points in the instrumentation process, thus enabling researchers to identify where the cracks started and their progression during the course of instrument use.

Brazilian Dental Journal, 2018

In order to evaluate the volume of dentinal cracks taking into account the age of the dentin and ... more In order to evaluate the volume of dentinal cracks taking into account the age of the dentin and the type of file system used for endodontic procedures, forty freshly extracted single-rooted lower incisive teeth presenting similar root volume, were divided into two groups according to the age of the patient: Group Young (18 - 30 years old) and Group Old (60 years old or more). Each specimen was scanned by microcomputed tomography (micro-CT) in three stages: (i) before any treatment, (ii) after endodontic treatment with Reciproc files (REC), and (iii) after subsequent endodontic retreatment. Each group was subdivided into two subgroups, according to the retreatment technique used: retreatment with REC or with ProTaper Universal Retreatment (PUR) files. For each subgroup, the images were analyzed for differences in the volume of dentinal cracks in the middle and apical thirds of the teeth , according to the dentin age. In both stages (before and after instrumentation), the micro-CT im...

Dental Materials, 2018

Objectives. The increased demand for esthetics and minimally invasive tooth restorations resulted... more Objectives. The increased demand for esthetics and minimally invasive tooth restorations resulted in a rapid development of adhesive dentistry. However, much controversy remains about the safe use and cytotoxic effects of different groups of dental adhesives. The present study performed a systematic review to identify the answer to the following question: are self-etch adhesives more cytotoxic than those employing the etch-and-rinse system? Methods. This systematic review was performed in accordance with the PRISMA statement; a quality assessment for in vitro studies was conducted using the ToxRTool. Specific search strategies were developed and performed in the electronic databases MEDLINE via PubMed, Cochrane Library, Scopus, Web of Science, and LILACS/BBO. After removal of duplicated studies and application of the exclusion criteria, ten eligible articles were selected and submitted to a qualitative descriptive analysis comparing both groups of dental adhesives. Most in vitro test systems employed pulp cells or gingival fibroblasts. Results. The methodologies presented great variability regarding the exposure to the test materials. Only four studies assessed the role of the degree of conversion of the materials in their toxicity, with conflicting results. Significance. While the lack of methodological standardization among the studies still hinders the establishment of a relationship between type of dental adhesive and toxicity, studies employing dentin barrier systems indicate greater cytotoxicity for etch-and-rinse adhesives.

Iranian endodontic journal, 2018

This study evaluated the removal of the filling material during endodontic retreatment considerin... more This study evaluated the removal of the filling material during endodontic retreatment considering the presence of cracks and the dentin age. A total of 20 freshly extracted single-rooted teeth were categorized into the following two groups according to the age of the patients: Group Young (Y; aged 18-30 years) and Group Old (O; aged ≥60 years). Each tooth specimen was scanned by microcomputed tomography (micro-CT) subsequently after endodontic retreatment with the Reciproc instruments (REC). The images were analyzed for differences in the volume of dentin cracks and the presence of the filling material in the middle and apical thirds of the teeth among the groups, according to the dentin age. The micro-CT images showed that after retreatment, there were more cracks in the old root dentin than those in the young root dentin, although the difference was not statistically significant (>0.05). The greatest reduction in the filling material was achieved when the old root dentin with ...

Journal of applied oral science : revista FOB, 2018

Denture adhesives (DA) improve the retention and stability of ill-fitting dentures, especially fo... more Denture adhesives (DA) improve the retention and stability of ill-fitting dentures, especially for older adults. These materials should be biocompatible, i.e., they cannot cause undesired biological responses and be non-cytotoxic to oral tissues. However, in vitro testing of DA biocompatibility employing primary cell culture may possibly be affected by other factors, such as the donor age. To compare the cytotoxicity of three different denture adhesives when assessed in primary gingival fibroblasts from a young donor or from an older donor, as well as the release of the basic fibroblast growth factor (bFGF), and the inflammatory response marker interleukin-6 (IL-6). Gingival fibroblasts isolated from a 30- and a 62-year-old donor were assayed for proliferation (1-7 days) and sensitivity to latex (positive control). Fibroblasts were indirectly exposed to Corega Ultra (cream), Corega powder and Fixodent Original for a 24 h period and assayed by XTT and Crystal Violet tests. The releas...

Brazilian oral research, Jan 28, 2017

The aim of this study was to undertake a qualitative and quantitative assessment of nanoscale alt... more The aim of this study was to undertake a qualitative and quantitative assessment of nanoscale alterations and wear on the surfaces of nickel-titanium (NiTi) endodontic instruments, before and after use, through a high-resolution, noncontact, three-dimensional optical profiler, and to verify the accuracy of the evaluation method. Cutting blade surfaces of two different brands of NiTi endodontic instruments, Reciproc R25 (n = 5) and WaveOne Primary (n = 5), were examined and compared before and after two uses in simulated root canals made in clear resin blocks. The analyses were performed on three-dimensional images which were obtained from surface areas measuring 211 × 211 µm, located 3 mm from their tips. The quantitative evaluation of the samples was conducted before and after the first and second usage, by the recordings of three amplitude parameters. The data were subjected to statistical analysis at a 5% level of significance. The results revealed statistically significant incre...

Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 2017

Objective: To compare the bond strength of three-step and self-etch adhesive systems with and wit... more Objective: To compare the bond strength of three-step and self-etch adhesive systems with and without prior acid etching on bonding of fiberglass posts in root canals. Material and Methods: Experiments were conducted on roots of five upper molars without curvature and sectioned in a cervical-apical direction. With the aid of a highspeed turbine and diamond tips, cylindrical cavities 7 mm in depth and 1.4 mm in diameter were made in the dentine. The roots of the same tooth were used, where the retainers were cemented, thus generating three groups: Group A: Adper Scotchbond Multipurpose Plus (SBMP) + Rely X ARC; Group B: Single Bond Universal (SBU-1) + Rely X ARC, with acid etching prior to adhesive insertion; Group C: Single Bond Universal (SBU-2) + Rely X ARC. The samples were stored in a bacteriological incubator at 37ºC with 100% humidity for 48h and were subsequently cross-sectioned to obtain 3 dentine discs 1mm in thickness. Forty-five specimens were subjected to the push-out test. Results: Through the analysis of variance and Tukey's test, it was found that SBMP showed, with statistical significance (p<0.05), highest bond strength when compared with the SBU-1 and SBU-2 systems. The observed difference in the discs was located in the cervical portion. Conclusion: The three-step adhesive system had higher bond strength with the substrate than the self-etching adhesive only in the disc in the cervical portion; further, statistically, the prior application of phosphoric acid in SBU-1 did not affect its bond strength significantly.

Brazilian dental journal

Bis-acryl resins are used for temporary dental restorations and have shown advantages over other ... more Bis-acryl resins are used for temporary dental restorations and have shown advantages over other materials. The aim of this work was to evaluate the in vitro cytotoxicity of two bis-acryl composite resins (Protemp 4 and Luxatemp Star), obtained at 1, 7 and 40 days after mixing the resin components, using a standardized assay employing human primary cells closely related to oral tissues. Human gingival fibroblast cell cultures were exposed for 24 h to either bis-acryl composite resins, polystyrene beads (negative control) and latex (positive control) extracts obtained after incubation by the different periods, at 37 °C under 5% CO2. Cell viability was evaluated using a multiparametric procedure involving sequential assessment (using the same cells) of mitochondrial activity (XTT assay), membrane integrity (neutral red test) and total cell density (crystal violet dye exclusion test). The cells exposed to the resin extracts showed cell viability indexes exceeding 75% after 24 h. Even w...

Iranian endodontic journal, 2016

The aim of this in vitro study was to evaluate the effect of 10% citric acid and 17% ethylenediam... more The aim of this in vitro study was to evaluate the effect of 10% citric acid and 17% ethylenediaminetetraacetic acid (EDTA) irrigating solutions on the surface morphology of young and old root dentin by determining the number and diameter of dentinal tubules using scanning electron microscopy (SEM). Fifty healthy human teeth collected from young (≤30 years) and old (≥60 years) individuals (n=25) were first prepared with a Largo bur #2 to produce smear layer on the root canal surface. Subsequently, the crowns and the root middle and apical thirds were sectioned and removed, and the cervical thirds were sectioned vertically in the buccal-lingual direction into two equal halves. The obtained samples were then immersed in 2.5% sodium hypochlorite for 30 min and randomly separated into two treatment groups for each age group. In each age group, ten samples were selected as controls and did not receive any type of treatment. The rest of the specimens were then rinsed, dried and treated fo...

The aim of this study was to evaluate comparatively the physical properties of solubility, disint... more The aim of this study was to evaluate comparatively the physical properties of solubility, disintegration and flow of some root canal sealers: SEALAPEX™, PULP CANAL SEALER™, TUBLISEAL™, AH PLUS™, AH 26™, TOP SEAL™, SEALER PLUS™, SEALER 26™ and ENDOFILL™. Method: Methodology used was according ISO 6786 which test the physicochemical properties using five proof specimens for each test. Results: In terms of solubility, the root canal sealers analyzed have been classified in the following decreasing order: ENDOFILL™, SEALAPEX™, PULP CANAL SEALER™, AH 26™, TUBLISEAL™, SEALER PLUS™, AH PLUS™, TOP SEAL™ and SEALER 26™, where a statistically significant difference was found at a 1% level. Between TOP SEAL™ and SEALER™ 26 root canal sealers there was no statistically significant difference. None of the sealers above have shown disintegration. Regarding flow, the root canal sealers in the study have been classified in the following decreasing order: TUBLISEAL™ root canal sealer, followed by ENDOFILL™, SEALAPEX™, PULP CANAL SEALER™, AH PLUS™, TOP SEAL™, AH 26™, SEALER 26™ and SEALER PLUS™, where a statistically significant difference was found at a 1% level. Conclusion: The solubility, only the SEALAPEX™ and ENDOFILL™ have shown a limit above determined by ISO 6786.

Brazilian Oral Research, 2015

The aim of this study was to comparatively evaluate the bending resistance at 45º, the static and... more The aim of this study was to comparatively evaluate the bending resistance at 45º, the static and dynamic cyclic fatigue life, and the fracture type of the WaveOne (Dentsply Maillefer, Ballaigues, Switzerland) 25-08 and Reciproc (VDW, Munich, Germany) 25-08 instruments. A total of 60 nickel-titanium (NiTi) instruments (30 Reciproc and 30 WaveOne) from three different lots, each of which was 25 mm in length, were tested. The bending resistance was evaluated through the results of a cantilever-bending test conducted using a universal testing machine. Static and dynamic cyclic fatigue testing was conducted using a custom-made device. For the static and dynamic tests, a cast Ni-Cr-Mo-Ti alloy metal block with an artificial canal measuring 1.77 mm in diameter and 20.00 mm in total length was used. A scanning electron microscope was used to determine the type of fracture. Statistical analyses were performed on the results. The WaveOne instrument was less flexible than the Reciproc (p < 0.05). The Reciproc instrument showed better resistance in the static and dynamic cyclic fatigue tests (p < 0.05). The transverse cross-section and geometry of the instruments were important factors in their resistance to bending and cyclic fracture. Both of the instruments showed ductile-type fracture characteristics. It can be concluded that the Reciproc 25-08 instrument was more resistant to static and dynamic cyclic fatigue than the WaveOne 25-08 instrument, while the WaveOne 25-08 instrument was less flexible. Bending and resistance to cyclic fracture were influenced by the instruments' geometries and transverse cross-sections. Both of the instruments showed ductile-type fracture characteristics.