Paolo Buracco - Academia.edu (original) (raw)

Papers by Paolo Buracco

Animals, 2021

Thymoma is a tumor rarely reported in dogs and should be differentiated from mediastinal lymphoma... more Thymoma is a tumor rarely reported in dogs and should be differentiated from mediastinal lymphoma. Clinical signs may have a late onset, and thymoma is often diagnosed when symptoms related to the space-occupying effect or paraneoplastic syndromes occur. CT and fine-needle aspirates or core biopsies are helpful in differential diagnosis, but flow cytometry may improve the pre-operative diagnostic ability. Concurrent paraneoplastic syndromes such as myasthenia gravis and hypercalcemia have been reported; however, their role as prognostic factors is not well determined. Surgical excision is the treatment of choice; adjuvant radiotherapy and/or chemotherapy may prolong survival in cases of incomplete excision or when a thymic carcinoma is diagnosed. Local recurrence and metastasis are infrequently reported; therefore, a long survival time is expected if the tumor is completely excised or if adjuvant therapy is undertaken. This article reports the authors’ experience with 28 dogs affect...

VII CONGRESSO NAZIONALE SOCIETÀ ITALIANA DI CHIRURGIA VETERINARIA (S.I.C.V.), 2000

Veterinary Surgical Oncology, 2022

Frontiers in Veterinary Science, 2022

In veterinary oncology, canine melanoma is still a fatal disease for which innovative and long-la... more In veterinary oncology, canine melanoma is still a fatal disease for which innovative and long-lasting curative treatments are urgently required. Considering the similarities between canine and human melanoma and the clinical revolution that immunotherapy has instigated in the treatment of human melanoma patients, special attention must be paid to advancements in tumor immunology research in the veterinary field. Herein, we aim to discuss the most relevant knowledge on the immune landscape of canine melanoma and the most promising immunotherapeutic approaches under investigation. Particular attention will be dedicated to anti-cancer vaccination, and, especially, to the encouraging clinical results that we have obtained with DNA vaccines directed against chondroitin sulfate proteoglycan 4 (CSPG4), which is an appealing tumor-associated antigen with a key oncogenic role in both canine and human melanoma. In parallel with advances in therapeutic options, progress in the identification ...

Veterinary and Comparative Orthopaedics and Traumatology, 2000

Six dogs with osteosarcoma of the radius were treated by local resection and limb salvage using b... more Six dogs with osteosarcoma of the radius were treated by local resection and limb salvage using bone transport osteogenesis. One case is described in detail. Although the initial learning curve is steep for this technique, strict adherence to oncologic surgical technique and the principles of Ilizarov can lead to a successful outcome. Bone transport shows promise as an alternative to cortical allografts for reconstruction of large bone defects after tumor resection in the canine radius.

Veterinary Pathology, 2005

Abdominal ultrasound examination in an 11-year-old, intact, female Labrador dog with hepatic dise... more Abdominal ultrasound examination in an 11-year-old, intact, female Labrador dog with hepatic disease revealed a nodular swelling of the left adrenal gland. Hyperadrenocorticism was suspected, but endocrine tests were negative. At the owner's request, an adrenalectomy was performed. Grossly, a nodular mass protruded from the external surface of the left adrenal gland and in cut section was hemorrhagic and effaced the cortical and medullary regions. Histologic examination revealed a cortical neoplasm with medullary involvement. The mass was composed of well-differentiated adipose cells, megakaryocytes, hematopoietic cells, and macrophages containing hemosiderin deposits. A diagnosis of cortical adrenal myelolipoma was made.

Journal of Comparative Pathology, 2012

Journal of Comparative Pathology, 2014

Archives on Veterinary Science and Technology, 2017

Background: Several studies show that anesthesia for primary cancer surgery might influence cance... more Background: Several studies show that anesthesia for primary cancer surgery might influence cancer recurrence regulating specific gene expression like the Neuroepithelial Transforming (NET) 1 protein. This gene has been associated with malignant behaviors and represents a novel prognostic marker in human epithelial cancers. The present study investigates the in vitro effects of a clinically available propofol formulation on NET1 expression in canine mammary tumor cells, as a potential translational model. Methods: Two canine mammary tumor cell lines, primary (CIPp) and metastatic (CIPm), were incubated with propofol (1-10 μg ml-1). Cells were lysate and RNA isolated at pre-established time points. A quantitative PCR was performed to evaluate NET1 gene expression and resulting delta cycle thresholds compared. Results: Baseline NET1 gene expression was lower in CIPm compared with CIPp. Both propofol concentrations increased NET1 mRNA levels in CIPp after 6 hours. In CIPm the higher propofol concentration caused a reduction in gene expression after 6 hours. Propofol decreased gene expression in both cell lines and only in CIPp after 12 and 24 hours, respectively. No differences were found in CIPm after 48 hours. The higher concentration of propofol increased gene expression in CIPp after 48 hours. Conclusions: Metastatic cells showed a lower basal NET1 expression and were less responsive to treatments compared to primary tumor cells. Propofol effectively influenced NET1 gene expression without a clear dose dependency. Most treatment time-points showed a decreased NET1 gene expression, although increases were also observed.

The Veterinary Journal, 2017

Combination toceranib and lomustine shows frequent high grade toxicities when used for treatment ... more Combination toceranib and lomustine shows frequent high grade toxicities when used for treatment of non-resectable or recurrent mast cell tumours in dogs: A European multicentre study (2010),

XII Congresso Nazionale S.I.Di.L.V., 2010

Veterinary and Comparative Oncology, 2021

Studies regarding the neoplastic infiltration of the skin overlying canine subcutaneous soft tiss... more Studies regarding the neoplastic infiltration of the skin overlying canine subcutaneous soft tissue sarcoma (sSTS) are lacking. In case of the absence of tumour infiltration, there would be the possibility of leaving this unaffected skin in place, thus simplifying surgery. The aim of the study was to investigate whether the skin overlying sSTSs is infiltrated by neoplastic cells. Dogs with sSTSs treated surgically were prospectively enrolled. After excision, the skin was dissected from the tumour along the natural surgical plane of cleavage and histologically evaluated. Twenty-nine dogs with an sSTS were included (22 grade I, 6 grade II and 1 grade III). The STS-overlying skin was not tumour-infiltrated in 14/29 cases (48.3%). A higher frequency of infiltration was observed in higher grade sSTSs (grades II and III, 100%; P = 0.006); nevertheless, 8/22 grade I STSs (36%) also showed cutaneous infiltration. This infiltration involved the dermis of the skin directly in contact with the tumour (multifocal in 11 and diffuse in 4 cases). Although the cutaneous tumour infiltration is less frequent in grade I sSTSs and a wide excision may still be the safest treatment for any sSTS for a greater possibility of local control, this study opens the possibility to a less aggressive cutaneous excision, but still with a local curative intent, as only the skin directly in contact with the sSTS has been proven to be tumour-infiltrated. Additional studies are warranted to confirm that excision of only this skin may guarantee a complete local control, especially in lower-grade sSTSs. This article is protected by copyright. All rights reserved.