Parisa Mohamadynejad - Academia.edu (original) (raw)

Papers by Parisa Mohamadynejad

Research paper thumbnail of Comparative Effects of Ginseng Extract and Green Tea with Pomeol Bruler on C-Reactive Protein and Liver Enzymes of Rats Fed a High-Fat Diet

DOAJ (DOAJ: Directory of Open Access Journals), Jul 1, 2022

Research paper thumbnail of Types of Cell Death from a Molecular Perspective

Biology

The former conventional belief was that cell death resulted from either apoptosis or necrosis; ho... more The former conventional belief was that cell death resulted from either apoptosis or necrosis; however, in recent years, different pathways through which a cell can undergo cell death have been discovered. Various types of cell death are distinguished by specific morphological alterations in the cell’s structure, coupled with numerous biological activation processes. Various diseases, such as cancers, can occur due to the accumulation of damaged cells in the body caused by the dysregulation and failure of cell death. Thus, comprehending these cell death pathways is crucial for formulating effective therapeutic strategies. We focused on providing a comprehensive overview of the existing literature pertaining to various forms of cell death, encompassing apoptosis, anoikis, pyroptosis, NETosis, ferroptosis, autophagy, entosis, methuosis, paraptosis, mitoptosis, parthanatos, necroptosis, and necrosis.

Research paper thumbnail of Identification of lncRNA PCAT19 as potential novel biomarker for colorectal cancer

Research paper thumbnail of Differential expression of KCNQ1 and ATP4A genes according to the sex and age in the stomach

Nucleosides, Nucleotides & Nucleic Acids

Research paper thumbnail of Association of Val660Leu, progesterone receptor polymorphic variant, with susceptibility to RRMS disease

Research paper thumbnail of Significant Association of rs77493513 Polymorphism in 3'-UTR of the NRG1 Gene with the Risk of Multiple Sclerosis Disease

Metabolic Brain Disease

Multiple sclerosis (MS) is a chronic inflammatory and autoimmune disease characterized by demyeli... more Multiple sclerosis (MS) is a chronic inflammatory and autoimmune disease characterized by demyelination of the central nervous system (CNS). Neuregulin 1 (NRG1) is a signaling protein that plays an important role in a variety of biological processes, including potentiate oligodendrocyte differentiation and myelination in the CNS, immune response regulation, and inflammation. Single nucleotide polymorphism (SNP) rs77493513 is located in the untranslated region of the 3' mRNA (3'-UTR) of the NRG1 gene, which is predicted to be the binding site of several microRNAs and may play an important role in post-transcriptional regulation. Study aimed to investigate the association of SNP rs77493513 in the NRG1 gene with the risk of MS disease. In this study, genomic DNA was extracted from whole blood samples of 182 patients with relapsing-remitting multiple sclerosis (RRMS) and 198 controls. Different genotypes of rs77493513 polymorphism were determined using RFLP-PCR technique. Statistical analysis was performed using SPSS 21.0 software and by t, χ2 and logistic regression tests. Our data showed that genotypes AC (OR=3.63, CI= 1.93-6.81, p<0.001) and CC (OR=7.90, CI= 4.13-15.11, p<0.001) significantly increased the risk of MS disease and C allele is risk allele. Also, AC (OR=0.16, CI= 0.04-0.63, p= 0.009) and CC (OR=0.14, CI= 0.03-0.53, p=0.04) genotypes significantly decrease the age of onset of the disease. The results show that allele C of rs77493513 polymorphism in the NRG1 gene can be a risk factor for MS.

Research paper thumbnail of Splicing in Cancer

Molecular Mechanisms in Cancer

Defects in splicing, especially alternative splicing have been frequently found in cancers. Mutat... more Defects in splicing, especially alternative splicing have been frequently found in cancers. Mutations in the splicing regulatory elements of important genes involved in cancers or the genes encoding regulatory splicing machinery could play a key role in carcinogenesis. Alterations in regulator factors in splicing have emerged as a new class of oncoproteins and tumor suppressor genes. Understanding the molecular mechanism of how defects in splicing and in particular alternative splicing are involved in carcinogenesis, could lead to new strategies to cancer therapy. Here, we review the molecular mechanism of splicing and regulatory factors involved in alternative splicing, as well as the aberrant splicing that affects cancer hallmarks. Finally, we summarize new approaches in cancer therapy based on splicing.

Research paper thumbnail of Significant Association of DNASE1 Variable Number Tandem Repeats and Single Nucleotide Polymorphisms With Gastric Cancer

British Journal of Biomedical Science

Introduction: Defects in the apoptotic process are among the most important events involved in ca... more Introduction: Defects in the apoptotic process are among the most important events involved in carcinogenesis, and defects in DNASE1, as one of the apoptotic machinery components, plays a role in various types of cancer. Previous studies have indicated significant differences in the DNASE1 polymorphisms in different populations. We hypothesized an association of two polymorphic sites in the exon 8 and the intron 4 of the DNASE1 gene with the risk of gastric cancer.Materials and Methods: The study was carried out on 120 gastric cancer patients and 120 age and sex adjusted controls using PCR and RFLP-PCR.Results: The genotype GG (rs1053874) in exon 8 of DNASE1 (odds ratio [95% confidence interval]) 4.65 [2.10–10.29], p < 0.001) and genotype 2/3 of variable number tandem repeat (VNTR) in the intron 4 (3.75 [1.56–9.01], p = 0.003) are both linked to gastric cancer.Conclusion: We propose that both polymorphic sites have a part to play in gastric cancer, and so may be useful diagnosis ...

Research paper thumbnail of Altered expression of Lnc-OC1 and SIRT1 genes in colorectal cancer tissue

Razi Journal of Medical Sciences, Jan 10, 2021

Research paper thumbnail of Association study of polymorphism in Thrombomodulin gene (rs1042579) with cardiovascular disease

Acta Bio Medica : Atenei Parmensis, 2022

Background and aim: Thrombomodulin (THBD) gene plays an important role in activation and control ... more Background and aim: Thrombomodulin (THBD) gene plays an important role in activation and control of protein C. Regulation protein C levels as an important risk factor for cardiovascular disease. Mutations in this gene can affect thrombomodulin levels. In this study, we aimed to investigate the role of rs1042579 SNP within THBD gene in patients with cardiovascular disease. Methods: The samples of this case-control study consisted of 105 Iranian patients with cardiovascular disease and 95 controls who were free of CVD events enrolled from March 2017 to December 2018 in this study. Demographic data, medical history, and para-clinical were measured. Genotyping was carried out using ARMS-PCR technique and Sanger sequencing was used. Twenty percent of the samples were sequenced to confirm the result of the genotyping. Results: Data analysis revealed that the rs1042579 within THBD gene was associated with a risk of cardiovascular disease. Molecular assay confirmed that TT genotype is a fac...

Research paper thumbnail of Increased risk of gastric cancer in the CC genotype -31C/G polymorphism in Survivin gene promoter

مجله دانشگاه علوم پزشکی گرگان, 2017

Background and Objective: Gastric cancer is the most common cancers worldwide. The survivin gene ... more Background and Objective: Gastric cancer is the most common cancers worldwide. The survivin gene which encodes an apoptosis protein inhibitor plays an important role in maintenance and integrity of the gastric mucosa. The gene is necessary for the normal physiologic function of the stomach, but its expression increases in gastric cancer. Regarding with the role of polymorphisms of the promoter region in genes expression, this study was done to determine the association of single- nucleotide polymorphism (rs9904341) -31C/G in promoter survivin gene with risk of gastric cancers. Methods: In this case-control study, 101 patients with gastric cancer and 101 matched age and gender healthy subjects as the control were examined by PCR-RFLP technique. Results: Genotype CC was significantly increased the risk of gastric cancer up to 2.4 folds (95% CI=1.03–5.61, P<0.04) and allele C, as risk allele, significantly increased the risk of gastric cancer up to 1.5 folds (95% CI=1.02–2.30, P<...

Research paper thumbnail of The association of rs10573247 polymorphism in 3ˈ-UTR of HMGA2 gene with risk of gastric cancer

New Cellular and Molecular Biotechnology Journal, 2019

Aim and Background: The HMGA2 protein is one of the transcription factors that regulates the evol... more Aim and Background: The HMGA2 protein is one of the transcription factors that regulates the evolution and differentiation of the cells. Studies have shown that changes in the expression of the HMGA2 gene can play an important role in preventing tumor progression and its metastasis. In this study, we investigated the association between rs10573247 polymorphism in the 3-UTR region of HMGA2 gene and the risk of gastric cancer. Material and Methods: Genomic DNA from blood sample of 100 patients with gastric cancer and 100 healthy individuals were extracted A part of the HMGA2 gene, including rs10573247 polymorphism, was amplified by PCR technique. After, the PCR product was treated with EaR1 enzyme and the genotype of each individual was determined. Finally, the risk of gastric cancer was assessed with the statistical tests of χ2 and logistic regression. Results: The statistical analysis of allelic and genotypic association of rs10573247 polymorphism with the risk of gastric cancer sho...

Research paper thumbnail of Poster Presentations: P-40: Association Assessment Between Promoter Polymorphism of MUC6 Gene and Susceptibility to Peptic Ulcer Disease

Research paper thumbnail of Cathepsin D gene Expression in Stomach: Its Association with Age, Sex, and Menopausal status

Journal of Fasa University of Medical Sciences, 2016

Research paper thumbnail of Assessment of association of A9194G polymorphism in the exon 4 eneg survivin with the gastric cancer risk

New Cellular and Molecular Biotechnology Journal, 2017

Research paper thumbnail of Increased Risk of Breast Cancer by Reducing the Number of Rhob Gene Promoter VNTR Repeats

Urmia medical journal, 2016

Research paper thumbnail of Increased Risk of Gastric Cancer with VNTR Polymorphism of SIRT3 Gene

Journal of Mazandaran University of Medical Sciences, 2017

Background and purpose: Gastric cancer is the most common cancer associated with high mortality w... more Background and purpose: Gastric cancer is the most common cancer associated with high mortality worldwide. One of the genes that is down-regulated in gastric cancer, is the SIRT3 that encodes the histone deacetylase enzyme. There is a variable number tandem repeat (VNTR) polymorphism in the intron 5 of SIRT3 gene and evidence shows that expression of SIRT3 gene increases by increase in the number of repeats. According to the deregulation of SIRT3 gene expression in gastric cancer and the effect of intron 5 VNTR polymorphism in the transcription, we investigated the association between intron 5 VNTR polymorphism of SIRT3 gene and the risk of developing gastric cancer. Materials and methods: A case-control study was performed in 116 patients with gastric cancer (attending Isfahan Omid Hospital, Iran) and healthy controls (n= 116). After DNA extraction, all samples were genotyped using PCR and electrophoresis techniques and the results were analyzed applying logistic regression and Chi...

Research paper thumbnail of Association of STin2 VNTR Polymorphism in the SLC6A4 Gene with Increased Risk of MS Disease

Alborz University Medical Journal, 2019

Research paper thumbnail of Evaluating the Immune Response of Recombinant H1N1 Hemagglutinin with MF59 Adjuvant in Animal Model as a Novel Alternative to the Influenza Vaccine

Iranian Journal of Allergy, Asthma and Immunology, 2020

The H1N1 influenza virus is known as a serious pandemic threat across the globe. Vaccination is o... more The H1N1 influenza virus is known as a serious pandemic threat across the globe. Vaccination is one of the most effective methods of protection against this virus and the way to reduce the seasonal pandemic risk. The commercial vaccine does not adequately respond to pandemic strains. This study examines the potential function of formulated H1N1 hemagglutinin with MF59 adjuvant against A/PR/8/34 (H1N1). To this end, a recombinant hemagglutinin (rHA) gene of influenza A virus was designed and expressed in SF9 cell by the Baculovirus expression system. Four groups of mice were immunized by rHA in combination with MF59, Alum adjuvant, and virus split only. The immunized mice subsequently used for the humoral immune assay and the results compared with untreated mice (negative group). Besides, both treated and control mice groups were challenged with mouse-adapted influenza virus A/PR/8/34(H1N1) through the intranasal drop. Bodyweight, survival, temperature variation, and the medical con...

Research paper thumbnail of A genetic variant in the flanking region of miR-182 could decrease the susceptibility to the breast cancer risk in the iranian population

Nucleosides, Nucleotides & Nucleic Acids, 2020

Research paper thumbnail of Comparative Effects of Ginseng Extract and Green Tea with Pomeol Bruler on C-Reactive Protein and Liver Enzymes of Rats Fed a High-Fat Diet

DOAJ (DOAJ: Directory of Open Access Journals), Jul 1, 2022

Research paper thumbnail of Types of Cell Death from a Molecular Perspective

Biology

The former conventional belief was that cell death resulted from either apoptosis or necrosis; ho... more The former conventional belief was that cell death resulted from either apoptosis or necrosis; however, in recent years, different pathways through which a cell can undergo cell death have been discovered. Various types of cell death are distinguished by specific morphological alterations in the cell’s structure, coupled with numerous biological activation processes. Various diseases, such as cancers, can occur due to the accumulation of damaged cells in the body caused by the dysregulation and failure of cell death. Thus, comprehending these cell death pathways is crucial for formulating effective therapeutic strategies. We focused on providing a comprehensive overview of the existing literature pertaining to various forms of cell death, encompassing apoptosis, anoikis, pyroptosis, NETosis, ferroptosis, autophagy, entosis, methuosis, paraptosis, mitoptosis, parthanatos, necroptosis, and necrosis.

Research paper thumbnail of Identification of lncRNA PCAT19 as potential novel biomarker for colorectal cancer

Research paper thumbnail of Differential expression of KCNQ1 and ATP4A genes according to the sex and age in the stomach

Nucleosides, Nucleotides & Nucleic Acids

Research paper thumbnail of Association of Val660Leu, progesterone receptor polymorphic variant, with susceptibility to RRMS disease

Research paper thumbnail of Significant Association of rs77493513 Polymorphism in 3'-UTR of the NRG1 Gene with the Risk of Multiple Sclerosis Disease

Metabolic Brain Disease

Multiple sclerosis (MS) is a chronic inflammatory and autoimmune disease characterized by demyeli... more Multiple sclerosis (MS) is a chronic inflammatory and autoimmune disease characterized by demyelination of the central nervous system (CNS). Neuregulin 1 (NRG1) is a signaling protein that plays an important role in a variety of biological processes, including potentiate oligodendrocyte differentiation and myelination in the CNS, immune response regulation, and inflammation. Single nucleotide polymorphism (SNP) rs77493513 is located in the untranslated region of the 3' mRNA (3'-UTR) of the NRG1 gene, which is predicted to be the binding site of several microRNAs and may play an important role in post-transcriptional regulation. Study aimed to investigate the association of SNP rs77493513 in the NRG1 gene with the risk of MS disease. In this study, genomic DNA was extracted from whole blood samples of 182 patients with relapsing-remitting multiple sclerosis (RRMS) and 198 controls. Different genotypes of rs77493513 polymorphism were determined using RFLP-PCR technique. Statistical analysis was performed using SPSS 21.0 software and by t, χ2 and logistic regression tests. Our data showed that genotypes AC (OR=3.63, CI= 1.93-6.81, p<0.001) and CC (OR=7.90, CI= 4.13-15.11, p<0.001) significantly increased the risk of MS disease and C allele is risk allele. Also, AC (OR=0.16, CI= 0.04-0.63, p= 0.009) and CC (OR=0.14, CI= 0.03-0.53, p=0.04) genotypes significantly decrease the age of onset of the disease. The results show that allele C of rs77493513 polymorphism in the NRG1 gene can be a risk factor for MS.

Research paper thumbnail of Splicing in Cancer

Molecular Mechanisms in Cancer

Defects in splicing, especially alternative splicing have been frequently found in cancers. Mutat... more Defects in splicing, especially alternative splicing have been frequently found in cancers. Mutations in the splicing regulatory elements of important genes involved in cancers or the genes encoding regulatory splicing machinery could play a key role in carcinogenesis. Alterations in regulator factors in splicing have emerged as a new class of oncoproteins and tumor suppressor genes. Understanding the molecular mechanism of how defects in splicing and in particular alternative splicing are involved in carcinogenesis, could lead to new strategies to cancer therapy. Here, we review the molecular mechanism of splicing and regulatory factors involved in alternative splicing, as well as the aberrant splicing that affects cancer hallmarks. Finally, we summarize new approaches in cancer therapy based on splicing.

Research paper thumbnail of Significant Association of DNASE1 Variable Number Tandem Repeats and Single Nucleotide Polymorphisms With Gastric Cancer

British Journal of Biomedical Science

Introduction: Defects in the apoptotic process are among the most important events involved in ca... more Introduction: Defects in the apoptotic process are among the most important events involved in carcinogenesis, and defects in DNASE1, as one of the apoptotic machinery components, plays a role in various types of cancer. Previous studies have indicated significant differences in the DNASE1 polymorphisms in different populations. We hypothesized an association of two polymorphic sites in the exon 8 and the intron 4 of the DNASE1 gene with the risk of gastric cancer.Materials and Methods: The study was carried out on 120 gastric cancer patients and 120 age and sex adjusted controls using PCR and RFLP-PCR.Results: The genotype GG (rs1053874) in exon 8 of DNASE1 (odds ratio [95% confidence interval]) 4.65 [2.10–10.29], p < 0.001) and genotype 2/3 of variable number tandem repeat (VNTR) in the intron 4 (3.75 [1.56–9.01], p = 0.003) are both linked to gastric cancer.Conclusion: We propose that both polymorphic sites have a part to play in gastric cancer, and so may be useful diagnosis ...

Research paper thumbnail of Altered expression of Lnc-OC1 and SIRT1 genes in colorectal cancer tissue

Razi Journal of Medical Sciences, Jan 10, 2021

Research paper thumbnail of Association study of polymorphism in Thrombomodulin gene (rs1042579) with cardiovascular disease

Acta Bio Medica : Atenei Parmensis, 2022

Background and aim: Thrombomodulin (THBD) gene plays an important role in activation and control ... more Background and aim: Thrombomodulin (THBD) gene plays an important role in activation and control of protein C. Regulation protein C levels as an important risk factor for cardiovascular disease. Mutations in this gene can affect thrombomodulin levels. In this study, we aimed to investigate the role of rs1042579 SNP within THBD gene in patients with cardiovascular disease. Methods: The samples of this case-control study consisted of 105 Iranian patients with cardiovascular disease and 95 controls who were free of CVD events enrolled from March 2017 to December 2018 in this study. Demographic data, medical history, and para-clinical were measured. Genotyping was carried out using ARMS-PCR technique and Sanger sequencing was used. Twenty percent of the samples were sequenced to confirm the result of the genotyping. Results: Data analysis revealed that the rs1042579 within THBD gene was associated with a risk of cardiovascular disease. Molecular assay confirmed that TT genotype is a fac...

Research paper thumbnail of Increased risk of gastric cancer in the CC genotype -31C/G polymorphism in Survivin gene promoter

مجله دانشگاه علوم پزشکی گرگان, 2017

Background and Objective: Gastric cancer is the most common cancers worldwide. The survivin gene ... more Background and Objective: Gastric cancer is the most common cancers worldwide. The survivin gene which encodes an apoptosis protein inhibitor plays an important role in maintenance and integrity of the gastric mucosa. The gene is necessary for the normal physiologic function of the stomach, but its expression increases in gastric cancer. Regarding with the role of polymorphisms of the promoter region in genes expression, this study was done to determine the association of single- nucleotide polymorphism (rs9904341) -31C/G in promoter survivin gene with risk of gastric cancers. Methods: In this case-control study, 101 patients with gastric cancer and 101 matched age and gender healthy subjects as the control were examined by PCR-RFLP technique. Results: Genotype CC was significantly increased the risk of gastric cancer up to 2.4 folds (95% CI=1.03–5.61, P<0.04) and allele C, as risk allele, significantly increased the risk of gastric cancer up to 1.5 folds (95% CI=1.02–2.30, P<...

Research paper thumbnail of The association of rs10573247 polymorphism in 3ˈ-UTR of HMGA2 gene with risk of gastric cancer

New Cellular and Molecular Biotechnology Journal, 2019

Aim and Background: The HMGA2 protein is one of the transcription factors that regulates the evol... more Aim and Background: The HMGA2 protein is one of the transcription factors that regulates the evolution and differentiation of the cells. Studies have shown that changes in the expression of the HMGA2 gene can play an important role in preventing tumor progression and its metastasis. In this study, we investigated the association between rs10573247 polymorphism in the 3-UTR region of HMGA2 gene and the risk of gastric cancer. Material and Methods: Genomic DNA from blood sample of 100 patients with gastric cancer and 100 healthy individuals were extracted A part of the HMGA2 gene, including rs10573247 polymorphism, was amplified by PCR technique. After, the PCR product was treated with EaR1 enzyme and the genotype of each individual was determined. Finally, the risk of gastric cancer was assessed with the statistical tests of χ2 and logistic regression. Results: The statistical analysis of allelic and genotypic association of rs10573247 polymorphism with the risk of gastric cancer sho...

Research paper thumbnail of Poster Presentations: P-40: Association Assessment Between Promoter Polymorphism of MUC6 Gene and Susceptibility to Peptic Ulcer Disease

Research paper thumbnail of Cathepsin D gene Expression in Stomach: Its Association with Age, Sex, and Menopausal status

Journal of Fasa University of Medical Sciences, 2016

Research paper thumbnail of Assessment of association of A9194G polymorphism in the exon 4 eneg survivin with the gastric cancer risk

New Cellular and Molecular Biotechnology Journal, 2017

Research paper thumbnail of Increased Risk of Breast Cancer by Reducing the Number of Rhob Gene Promoter VNTR Repeats

Urmia medical journal, 2016

Research paper thumbnail of Increased Risk of Gastric Cancer with VNTR Polymorphism of SIRT3 Gene

Journal of Mazandaran University of Medical Sciences, 2017

Background and purpose: Gastric cancer is the most common cancer associated with high mortality w... more Background and purpose: Gastric cancer is the most common cancer associated with high mortality worldwide. One of the genes that is down-regulated in gastric cancer, is the SIRT3 that encodes the histone deacetylase enzyme. There is a variable number tandem repeat (VNTR) polymorphism in the intron 5 of SIRT3 gene and evidence shows that expression of SIRT3 gene increases by increase in the number of repeats. According to the deregulation of SIRT3 gene expression in gastric cancer and the effect of intron 5 VNTR polymorphism in the transcription, we investigated the association between intron 5 VNTR polymorphism of SIRT3 gene and the risk of developing gastric cancer. Materials and methods: A case-control study was performed in 116 patients with gastric cancer (attending Isfahan Omid Hospital, Iran) and healthy controls (n= 116). After DNA extraction, all samples were genotyped using PCR and electrophoresis techniques and the results were analyzed applying logistic regression and Chi...

Research paper thumbnail of Association of STin2 VNTR Polymorphism in the SLC6A4 Gene with Increased Risk of MS Disease

Alborz University Medical Journal, 2019

Research paper thumbnail of Evaluating the Immune Response of Recombinant H1N1 Hemagglutinin with MF59 Adjuvant in Animal Model as a Novel Alternative to the Influenza Vaccine

Iranian Journal of Allergy, Asthma and Immunology, 2020

The H1N1 influenza virus is known as a serious pandemic threat across the globe. Vaccination is o... more The H1N1 influenza virus is known as a serious pandemic threat across the globe. Vaccination is one of the most effective methods of protection against this virus and the way to reduce the seasonal pandemic risk. The commercial vaccine does not adequately respond to pandemic strains. This study examines the potential function of formulated H1N1 hemagglutinin with MF59 adjuvant against A/PR/8/34 (H1N1). To this end, a recombinant hemagglutinin (rHA) gene of influenza A virus was designed and expressed in SF9 cell by the Baculovirus expression system. Four groups of mice were immunized by rHA in combination with MF59, Alum adjuvant, and virus split only. The immunized mice subsequently used for the humoral immune assay and the results compared with untreated mice (negative group). Besides, both treated and control mice groups were challenged with mouse-adapted influenza virus A/PR/8/34(H1N1) through the intranasal drop. Bodyweight, survival, temperature variation, and the medical con...

Research paper thumbnail of A genetic variant in the flanking region of miR-182 could decrease the susceptibility to the breast cancer risk in the iranian population

Nucleosides, Nucleotides & Nucleic Acids, 2020