Patric A Clapshaw - Academia.edu (original) (raw)

Patric A Clapshaw

Director of Solomon park research laboratories which specializes in the production of human serum and plasma calibrator and controls and stores research materials in ultra low freezers

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Papers by Patric A Clapshaw

Research paper thumbnail of Role of Normalization in the Elimination of Abundant Myelin Sequences in Spinal Cord cDNA Libraries Produced by Suppression Subtractive Hybridization

Cellular and Molecular Neurobiology, May 9, 2009

Research paper thumbnail of Add Health Wave IV Documentation: Lipids

Research paper thumbnail of Add Health Wave IV Documentation Report

Research paper thumbnail of An updated protocol based on CLSI document C37 for preparation of off-the-clot serum from individual units for use alone or to prepare commutable pooled serum reference materials

Clinical Chemistry and Laboratory Medicine (CCLM)

Manufacturers of in vitro diagnostic medical devices, clinical laboratories, research laboratorie... more Manufacturers of in vitro diagnostic medical devices, clinical laboratories, research laboratories and calibration laboratories require commutable reference materials that can be used in the calibration hierarchies of medical laboratory measurement procedures used for human specimens to establish metrological traceability to higher order reference systems. Commutable materials are also useful in external quality assessment surveys. In order to achieve these goals, matrix-based reference materials with long-term stability, appropriate measurand concentrations and commutability with individual human specimens are required. The Clinical and Laboratory Standards Institute (CLSI) guideline C37-A (now archived) provided guidance to prepare commutable pooled serum reference materials for use in the calibration hierarchies of cholesterol measurement procedures. Experience using the C37-A guideline has identified a number of technical enhancements as well as applications to measurands other ...

Research paper thumbnail of Availability of plasma with target values for certain lipids

Research paper thumbnail of Zero-bias cholesterol and triglyceride proficiency survey

Research paper thumbnail of Development of oligodendrocytes and Schwann cells studied with a monoclonal antibody against galactocerebroside

Proceedings of the National Academy of Sciences, 1982

Research paper thumbnail of Characterization of 2':3'-Cyclic Nucleotide 3'-Phosphodiesterase: Rapid Isolation, Native Enzyme Analysis, Identification of a Serum-Soluble Activity, and Kinetics

Journal of Neurochemistry, 1981

Research paper thumbnail of Effects of Detergents, Proteins, and Lipids on 2':3'-Cyclic-Nucleotide 3'-Phosphodiesterase Activity

Journal of Neurochemistry, 1980

Research paper thumbnail of Intracellular Localization of 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase in a Neuronal Cell Line as Examined by Immunofluorescence and Cell Fractionation

Journal of Neurochemistry, 1981

Research paper thumbnail of Two molecular forms of the isolated brain enzyme 2′,3′-cyclic nucleotide 3′-phosphodiesterase

Research paper thumbnail of Role of Normalization in the Elimination of Abundant Myelin Sequences in Spinal Cord cDNA Libraries Produced by Suppression Subtractive Hybridization

Cellular and Molecular Neurobiology, 2009

Research paper thumbnail of Murine Spinal Cord Transcriptome Analysis Following Reduction of Prevalent Myelin cDNA Sequences

Cellular and Molecular Neurobiology, 2009

Research paper thumbnail of Spinal Cord Transcriptome Analysis Using Suppression Subtractive Hybridization and Mirror Orientation Selection

Cellular and Molecular Neurobiology, 2006

Comparison of cDNA libraries derived from the spinal cord with those derived from the visual cort... more Comparison of cDNA libraries derived from the spinal cord with those derived from the visual cortex by means of forward and reverse subtractive hybridization resulted in the cataloguing of 60 genes differentially expressed in the spinal cord. 1. The differentially expressed genes represent a mixture of novel and known sequences with known and unknown protein products. 2. The possibility that the subtraction process was simply overwhelmed by background sequences was significantly reduced by several observations including comparisons between suppression subtractive hybridization (SSH) and mirror orientation selection (MOS). 3. Nearly half of all genes up-regulated in the spinal cord are of myelin origin. 4. Twenty-five percent of all up-regulated clones in the spinal cord versus the visual cortex are for proteolipid protein. 5. Ten percent of all up-regulated clones in spinal cord versus visual cortex are for ferretin heavy chain, which is known to be produced in oligodendroglial cells in the CNS. 6. Two of the up-regulated sequences, proteolipid protein and N-myc down-regulated gene 4, are identified with genes known to directly affect neuron survival. 7. Two of the up-regulated genes, ferritin and transferrin, are indirectly associated with apoptosis through their ability to sequester iron and reduce free radical formation.

Research paper thumbnail of Simultaneous Measurement of 2':3' Cyclic-Nucleotide 3' Phosphodiesterase and RNase Activities in Sera and Spinal Fluids of Multiple Sclerosis Patients

Journal of Neurochemistry, 1984

The enzymes 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and RNa... more The enzymes 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and RNase were simultaneously measured in the sera and CSF of multiple sclerosis (MS) and non-MS patients. No evidence of increased activity for these enzymes could be found regardless of pathology in either fluid source. Discrepancies between the present results and those from two previous studies that reported significant increases in CNPase activity in the CSF of patients with MS were carefully analyzed. It was concluded that the apparent increased CNPase activity correlated with MS in both previous studies was most probably the result of methodological and computational difficulties.

Research paper thumbnail of Role of Normalization in the Elimination of Abundant Myelin Sequences in Spinal Cord cDNA Libraries Produced by Suppression Subtractive Hybridization

Cellular and Molecular Neurobiology, May 9, 2009

Research paper thumbnail of Add Health Wave IV Documentation: Lipids

Research paper thumbnail of Add Health Wave IV Documentation Report

Research paper thumbnail of An updated protocol based on CLSI document C37 for preparation of off-the-clot serum from individual units for use alone or to prepare commutable pooled serum reference materials

Clinical Chemistry and Laboratory Medicine (CCLM)

Manufacturers of in vitro diagnostic medical devices, clinical laboratories, research laboratorie... more Manufacturers of in vitro diagnostic medical devices, clinical laboratories, research laboratories and calibration laboratories require commutable reference materials that can be used in the calibration hierarchies of medical laboratory measurement procedures used for human specimens to establish metrological traceability to higher order reference systems. Commutable materials are also useful in external quality assessment surveys. In order to achieve these goals, matrix-based reference materials with long-term stability, appropriate measurand concentrations and commutability with individual human specimens are required. The Clinical and Laboratory Standards Institute (CLSI) guideline C37-A (now archived) provided guidance to prepare commutable pooled serum reference materials for use in the calibration hierarchies of cholesterol measurement procedures. Experience using the C37-A guideline has identified a number of technical enhancements as well as applications to measurands other ...

Research paper thumbnail of Availability of plasma with target values for certain lipids

Research paper thumbnail of Zero-bias cholesterol and triglyceride proficiency survey

Research paper thumbnail of Development of oligodendrocytes and Schwann cells studied with a monoclonal antibody against galactocerebroside

Proceedings of the National Academy of Sciences, 1982

Research paper thumbnail of Characterization of 2':3'-Cyclic Nucleotide 3'-Phosphodiesterase: Rapid Isolation, Native Enzyme Analysis, Identification of a Serum-Soluble Activity, and Kinetics

Journal of Neurochemistry, 1981

Research paper thumbnail of Effects of Detergents, Proteins, and Lipids on 2':3'-Cyclic-Nucleotide 3'-Phosphodiesterase Activity

Journal of Neurochemistry, 1980

Research paper thumbnail of Intracellular Localization of 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase in a Neuronal Cell Line as Examined by Immunofluorescence and Cell Fractionation

Journal of Neurochemistry, 1981

Research paper thumbnail of Two molecular forms of the isolated brain enzyme 2′,3′-cyclic nucleotide 3′-phosphodiesterase

Research paper thumbnail of Role of Normalization in the Elimination of Abundant Myelin Sequences in Spinal Cord cDNA Libraries Produced by Suppression Subtractive Hybridization

Cellular and Molecular Neurobiology, 2009

Research paper thumbnail of Murine Spinal Cord Transcriptome Analysis Following Reduction of Prevalent Myelin cDNA Sequences

Cellular and Molecular Neurobiology, 2009

Research paper thumbnail of Spinal Cord Transcriptome Analysis Using Suppression Subtractive Hybridization and Mirror Orientation Selection

Cellular and Molecular Neurobiology, 2006

Comparison of cDNA libraries derived from the spinal cord with those derived from the visual cort... more Comparison of cDNA libraries derived from the spinal cord with those derived from the visual cortex by means of forward and reverse subtractive hybridization resulted in the cataloguing of 60 genes differentially expressed in the spinal cord. 1. The differentially expressed genes represent a mixture of novel and known sequences with known and unknown protein products. 2. The possibility that the subtraction process was simply overwhelmed by background sequences was significantly reduced by several observations including comparisons between suppression subtractive hybridization (SSH) and mirror orientation selection (MOS). 3. Nearly half of all genes up-regulated in the spinal cord are of myelin origin. 4. Twenty-five percent of all up-regulated clones in the spinal cord versus the visual cortex are for proteolipid protein. 5. Ten percent of all up-regulated clones in spinal cord versus visual cortex are for ferretin heavy chain, which is known to be produced in oligodendroglial cells in the CNS. 6. Two of the up-regulated sequences, proteolipid protein and N-myc down-regulated gene 4, are identified with genes known to directly affect neuron survival. 7. Two of the up-regulated genes, ferritin and transferrin, are indirectly associated with apoptosis through their ability to sequester iron and reduce free radical formation.

Research paper thumbnail of Simultaneous Measurement of 2':3' Cyclic-Nucleotide 3' Phosphodiesterase and RNase Activities in Sera and Spinal Fluids of Multiple Sclerosis Patients

Journal of Neurochemistry, 1984

The enzymes 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and RNa... more The enzymes 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and RNase were simultaneously measured in the sera and CSF of multiple sclerosis (MS) and non-MS patients. No evidence of increased activity for these enzymes could be found regardless of pathology in either fluid source. Discrepancies between the present results and those from two previous studies that reported significant increases in CNPase activity in the CSF of patients with MS were carefully analyzed. It was concluded that the apparent increased CNPase activity correlated with MS in both previous studies was most probably the result of methodological and computational difficulties.

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