Patrick Billard - Academia.edu (original) (raw)
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Papers by Patrick Billard
Environmental science & technology, Jan 19, 2014
Bioavailability of trace metals is a key parameter for assessment of toxicity on living organisms... more Bioavailability of trace metals is a key parameter for assessment of toxicity on living organisms. Proper evaluation of metal bioavailability requires monitoring the various interfacial processes that control metal partitioning dynamics at the biointerface, which includes metal transport from solution to cell membrane, adsorption at the biosurface, internalization and possible excretion. In this work, a methodology is proposed to quantitatively describe the dynamics of Cd(II) uptake by Pseudomonas putida. The analysis is based on the kinetic measurement of Cd(II) depletion from bulk solution at various initial cell concentrations using electroanalytical probes. On the basis of a recent formalism on dynamics of metal uptake by complex biointerphases, cell concentration-dependent depletion timescale and plateau value reached by metal concentration at long exposure times (>3 hrs) are successfully rationalized in terms of limiting metal uptake flux, rate of excretion and metal affini...
Environmental science & technology, Jan 19, 2014
Bioavailability of trace metals is a key parameter for assessment of toxicity on living organisms... more Bioavailability of trace metals is a key parameter for assessment of toxicity on living organisms. Proper evaluation of metal bioavailability requires monitoring the various interfacial processes that control metal partitioning dynamics at the biointerface, which includes metal transport from solution to cell membrane, adsorption at the biosurface, internalization and possible excretion. In this work, a methodology is proposed to quantitatively describe the dynamics of Cd(II) uptake by Pseudomonas putida. The analysis is based on the kinetic measurement of Cd(II) depletion from bulk solution at various initial cell concentrations using electroanalytical probes. On the basis of a recent formalism on dynamics of metal uptake by complex biointerphases, cell concentration-dependent depletion timescale and plateau value reached by metal concentration at long exposure times (>3 hrs) are successfully rationalized in terms of limiting metal uptake flux, rate of excretion and metal affini...