Paula Coble - Academia.edu (original) (raw)

Papers by Paula Coble

Research paper thumbnail of Vertical distribution of dissolved and particulate fluorescence in the Black Sea

Deep Sea Research Part A. Oceanographic Research Papers, 1991

Research paper thumbnail of The Nature and Distribution of Fluorescent Dissolved Organic Matter in the Black Sea and the Cariaco Trench

Black Sea Oceanography, 1991

Depth profiles of some of the chemical components of dissolved fluorescence in the Black Sea and ... more Depth profiles of some of the chemical components of dissolved fluorescence in the Black Sea and the Cariaco Trench were measured using ion-pairing high performance liquid chromatography. Both individual compounds and unresolvable complex material were observed in the fraction retained by C18-bonded silica adsorbent. Identifiable individual compounds included riboflavin, lumiflavin, lumichrome, FAD, and FMN. Maxima in riboflavin concentrations occurred in

Research paper thumbnail of Improving Ocean Education by Implementing the U.S. Ocean Action Plan's Directives

Marine Technology Society Journal, 2005

Research paper thumbnail of Monsoons boost biological productivity in Arabian Sea

Research paper thumbnail of Examining CDOM fluorescence variability using principal component analysis: seasonal and regional modeling of three-dimensional fluorescence in the Gulf of Mexico

Marine Chemistry, 2004

Alterations in colored dissolved organic matter (CDOM) characteristics were examined for an exten... more Alterations in colored dissolved organic matter (CDOM) characteristics were examined for an extensive fluorescence data set generated by excitation emission matrix spectroscopy (EEMS). Principal component analysis (PCA) was used to assess changes in fluorescence bandwidth and wavelength shifts of entire peak regions, enhancing previous CDOM characterization that was based on changes observed at a few excitation and emission wavelength pairs.

Research paper thumbnail of Characterization of Colored Dissolved Organic Matter Using High-Energy Laser Fragmentation

Environmental Science & Technology, 2000

Research paper thumbnail of Characterization of marine and terrestrial DOM in seawater using excitation-emission matrix spectroscopy

Marine Chemistry, 1996

High-resolution fluorescence spectroscopy was used to characterize dissolved organic matter (DOM)... more High-resolution fluorescence spectroscopy was used to characterize dissolved organic matter (DOM) in concentrated and unconcentrated water samples from a wide variety of freshwater, coastal and marine environments. Several types of fluorescent signals were observed, including humic-like, tyrosine-like, and tryptophan-like. Humic-like fluorescence consisted of two peaks, one stimulated by UV excitation (peak A) and one by visible excitation (peak C). For

Research paper thumbnail of Multispectral in situ measurements of organic matter and chlorophyll fluorescence in seawater: Documenting the intrusion of the Mississippi River plume in the West Florida Shelf

Limnology and Oceanography, 2001

Page 1. 1836 Notes , AND A. BURD. 1998. Seasonal size spectra of transparent exopolymeric particl... more Page 1. 1836 Notes , AND A. BURD. 1998. Seasonal size spectra of transparent exopolymeric particles (TEP) in a coastal sea and comparison with those predicted using coagulation theory. Mar. Ecol. Prog. Ser. 163: 63-76. , AND T. KI0RBOE. 1996. ...

Research paper thumbnail of Colored Dissolved Organic Matter in the Coastal Ocean: An Optical Tool for Coastal Zone Environmental Assessment and Management

Oceanography, 2004

... CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 0601153N 6. AUTHOR(S) Paula Coble... more ... CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 0601153N 6. AUTHOR(S) Paula Coble, Chuanmin Hu, Richard W. Gould, Jr., Grace Chang, and A. Michelle Wood 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. ...

Research paper thumbnail of Verification of mid-ocean ballast water exchange using naturally occurring coastal tracers

Marine Pollution Bulletin, 2004

We examined methods for verifying whether or not ships have performed mid-ocean ballast water exc... more We examined methods for verifying whether or not ships have performed mid-ocean ballast water exchange (BWE) on four commercial vessels operating in the Pacific and Atlantic Oceans. During BWE, a ship replaces the coastal water in its ballast tanks with water drawn from the open ocean, which is considered to harbor fewer organisms capable of establishing in coastal environments. We measured concentrations of several naturally occurring chemical tracers (salinity, six trace elements, colored dissolved organic matter fluorescence and radium isotopes) along ocean transects and in ballast tanks subjected to varying degrees of BWE (0-99%). Many coastal tracers showed significant concentration changes due to BWE, and our ability to detect differences between exchanged and unexchanged ballast tanks was greatest under multivariate analysis. An expanded dataset, which includes additional geographic regions, is now needed to test the generality of our results.

Research paper thumbnail of Optical properties of colored dissolved organic matter in the Northern Gulf of Mexico

Research paper thumbnail of Examining CDOM fluorescence variability using principal component analysis: seasonal and regional modeling of three-dimensional fluorescence in the Gulf of Mexico

Marine Chemistry, 2004

Alterations in colored dissolved organic matter (CDOM) characteristics were examined for an exten... more Alterations in colored dissolved organic matter (CDOM) characteristics were examined for an extensive fluorescence data set generated by excitation emission matrix spectroscopy (EEMS). Principal component analysis (PCA) was used to assess changes in ...

Research paper thumbnail of Chromophoric dissolved organic matter (CDOM) source characterization in the Louisiana Bight

Marine Chemistry, 2004

Chromophoric dissolved organic matter (CDOM) in the Mississippi plume region may have several dis... more Chromophoric dissolved organic matter (CDOM) in the Mississippi plume region may have several distinct sources: riverine (terrestrial soils), wetland (terrestrial plants), biological production (phytoplankton, zooplankton, microbial), and sediments. Complex mixing, ...

Research paper thumbnail of CDOM in the ocean: transformation processes and their effects on optical properties

Research paper thumbnail of Analysis of the optical properties of the Orinoco River plume by absorption and fluorescence spectroscopy

Research paper thumbnail of On the dispersal of riverine colored dissolved organic matter over the West Florida Shelf

Limnology and Oceanography, 2000

Research paper thumbnail of Influence of extreme storm events on West Florida Shelf CDOM distributions

Journal of Geophysical Research, 2009

Research paper thumbnail of Fluorescence detection of proteins and amino acids in capillary electrophoresis using a post-column sheath flow reactor

Journal of Chromatography A, 1998

A simple laser-induced fluorescence detection method for proteins and amino acids in capillary el... more A simple laser-induced fluorescence detection method for proteins and amino acids in capillary electrophoresis is reported. A sheath flow cell is utilized as a post-column reactor for fluorescence derivatization of proteins and amino acids by addition of o-phthaldialdehyde-2-mercaptoethanol to the sheath fluid. With the use of a 50 microns I.D. capillary, the limits of detection for carbonic anhydrase are 0.73 nM or 1.8 amol which represents a five- and two-fold improvement, respectively, over the best results previously reported for post-column detection. In addition, separation efficiencies up to 8.07 x 10(5) are achieved and the detector response is linear over three-orders of magnitude. These results demonstrate that mixing is adequate and the reaction kinetics are rapid enough to provide sensitive detection with this approach. Also, because this post-column derivatization scheme requires no instrumental changes to a typical sheath flow cell detector, the system can be used for detection of pre-column labeled analytes and for native fluorescence detection.

Research paper thumbnail of Monsoons boost biological productivity in arabian sea

Eos, Transactions American Geophysical Union, 1998

Monsoons over the Arabian Sea---the oceanic basin that separates the Arabian peninsula from the I... more Monsoons over the Arabian Sea---the oceanic basin that separates the Arabian peninsula from the Indian subcontinent---follow seasonal cycles, reversing directions twice a year, in summer and winter. Rather than spreading across the expanse of the sea, the southwest (summer) monsoon is often concentrated into a jet over the central Arabian Sea. Evidence suggests that variations in wind stress force substantial

Research paper thumbnail of Dispersal of the Suwannee River plume over the West Florida shelf: Simulation and observation of the optical and biochemical consequences of a flushing event

Geophysical Research Letters, 2003

Research paper thumbnail of Vertical distribution of dissolved and particulate fluorescence in the Black Sea

Deep Sea Research Part A. Oceanographic Research Papers, 1991

Research paper thumbnail of The Nature and Distribution of Fluorescent Dissolved Organic Matter in the Black Sea and the Cariaco Trench

Black Sea Oceanography, 1991

Depth profiles of some of the chemical components of dissolved fluorescence in the Black Sea and ... more Depth profiles of some of the chemical components of dissolved fluorescence in the Black Sea and the Cariaco Trench were measured using ion-pairing high performance liquid chromatography. Both individual compounds and unresolvable complex material were observed in the fraction retained by C18-bonded silica adsorbent. Identifiable individual compounds included riboflavin, lumiflavin, lumichrome, FAD, and FMN. Maxima in riboflavin concentrations occurred in

Research paper thumbnail of Improving Ocean Education by Implementing the U.S. Ocean Action Plan's Directives

Marine Technology Society Journal, 2005

Research paper thumbnail of Monsoons boost biological productivity in Arabian Sea

Research paper thumbnail of Examining CDOM fluorescence variability using principal component analysis: seasonal and regional modeling of three-dimensional fluorescence in the Gulf of Mexico

Marine Chemistry, 2004

Alterations in colored dissolved organic matter (CDOM) characteristics were examined for an exten... more Alterations in colored dissolved organic matter (CDOM) characteristics were examined for an extensive fluorescence data set generated by excitation emission matrix spectroscopy (EEMS). Principal component analysis (PCA) was used to assess changes in fluorescence bandwidth and wavelength shifts of entire peak regions, enhancing previous CDOM characterization that was based on changes observed at a few excitation and emission wavelength pairs.

Research paper thumbnail of Characterization of Colored Dissolved Organic Matter Using High-Energy Laser Fragmentation

Environmental Science & Technology, 2000

Research paper thumbnail of Characterization of marine and terrestrial DOM in seawater using excitation-emission matrix spectroscopy

Marine Chemistry, 1996

High-resolution fluorescence spectroscopy was used to characterize dissolved organic matter (DOM)... more High-resolution fluorescence spectroscopy was used to characterize dissolved organic matter (DOM) in concentrated and unconcentrated water samples from a wide variety of freshwater, coastal and marine environments. Several types of fluorescent signals were observed, including humic-like, tyrosine-like, and tryptophan-like. Humic-like fluorescence consisted of two peaks, one stimulated by UV excitation (peak A) and one by visible excitation (peak C). For

Research paper thumbnail of Multispectral in situ measurements of organic matter and chlorophyll fluorescence in seawater: Documenting the intrusion of the Mississippi River plume in the West Florida Shelf

Limnology and Oceanography, 2001

Page 1. 1836 Notes , AND A. BURD. 1998. Seasonal size spectra of transparent exopolymeric particl... more Page 1. 1836 Notes , AND A. BURD. 1998. Seasonal size spectra of transparent exopolymeric particles (TEP) in a coastal sea and comparison with those predicted using coagulation theory. Mar. Ecol. Prog. Ser. 163: 63-76. , AND T. KI0RBOE. 1996. ...

Research paper thumbnail of Colored Dissolved Organic Matter in the Coastal Ocean: An Optical Tool for Coastal Zone Environmental Assessment and Management

Oceanography, 2004

... CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 0601153N 6. AUTHOR(S) Paula Coble... more ... CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 0601153N 6. AUTHOR(S) Paula Coble, Chuanmin Hu, Richard W. Gould, Jr., Grace Chang, and A. Michelle Wood 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. ...

Research paper thumbnail of Verification of mid-ocean ballast water exchange using naturally occurring coastal tracers

Marine Pollution Bulletin, 2004

We examined methods for verifying whether or not ships have performed mid-ocean ballast water exc... more We examined methods for verifying whether or not ships have performed mid-ocean ballast water exchange (BWE) on four commercial vessels operating in the Pacific and Atlantic Oceans. During BWE, a ship replaces the coastal water in its ballast tanks with water drawn from the open ocean, which is considered to harbor fewer organisms capable of establishing in coastal environments. We measured concentrations of several naturally occurring chemical tracers (salinity, six trace elements, colored dissolved organic matter fluorescence and radium isotopes) along ocean transects and in ballast tanks subjected to varying degrees of BWE (0-99%). Many coastal tracers showed significant concentration changes due to BWE, and our ability to detect differences between exchanged and unexchanged ballast tanks was greatest under multivariate analysis. An expanded dataset, which includes additional geographic regions, is now needed to test the generality of our results.

Research paper thumbnail of Optical properties of colored dissolved organic matter in the Northern Gulf of Mexico

Research paper thumbnail of Examining CDOM fluorescence variability using principal component analysis: seasonal and regional modeling of three-dimensional fluorescence in the Gulf of Mexico

Marine Chemistry, 2004

Alterations in colored dissolved organic matter (CDOM) characteristics were examined for an exten... more Alterations in colored dissolved organic matter (CDOM) characteristics were examined for an extensive fluorescence data set generated by excitation emission matrix spectroscopy (EEMS). Principal component analysis (PCA) was used to assess changes in ...

Research paper thumbnail of Chromophoric dissolved organic matter (CDOM) source characterization in the Louisiana Bight

Marine Chemistry, 2004

Chromophoric dissolved organic matter (CDOM) in the Mississippi plume region may have several dis... more Chromophoric dissolved organic matter (CDOM) in the Mississippi plume region may have several distinct sources: riverine (terrestrial soils), wetland (terrestrial plants), biological production (phytoplankton, zooplankton, microbial), and sediments. Complex mixing, ...

Research paper thumbnail of CDOM in the ocean: transformation processes and their effects on optical properties

Research paper thumbnail of Analysis of the optical properties of the Orinoco River plume by absorption and fluorescence spectroscopy

Research paper thumbnail of On the dispersal of riverine colored dissolved organic matter over the West Florida Shelf

Limnology and Oceanography, 2000

Research paper thumbnail of Influence of extreme storm events on West Florida Shelf CDOM distributions

Journal of Geophysical Research, 2009

Research paper thumbnail of Fluorescence detection of proteins and amino acids in capillary electrophoresis using a post-column sheath flow reactor

Journal of Chromatography A, 1998

A simple laser-induced fluorescence detection method for proteins and amino acids in capillary el... more A simple laser-induced fluorescence detection method for proteins and amino acids in capillary electrophoresis is reported. A sheath flow cell is utilized as a post-column reactor for fluorescence derivatization of proteins and amino acids by addition of o-phthaldialdehyde-2-mercaptoethanol to the sheath fluid. With the use of a 50 microns I.D. capillary, the limits of detection for carbonic anhydrase are 0.73 nM or 1.8 amol which represents a five- and two-fold improvement, respectively, over the best results previously reported for post-column detection. In addition, separation efficiencies up to 8.07 x 10(5) are achieved and the detector response is linear over three-orders of magnitude. These results demonstrate that mixing is adequate and the reaction kinetics are rapid enough to provide sensitive detection with this approach. Also, because this post-column derivatization scheme requires no instrumental changes to a typical sheath flow cell detector, the system can be used for detection of pre-column labeled analytes and for native fluorescence detection.

Research paper thumbnail of Monsoons boost biological productivity in arabian sea

Eos, Transactions American Geophysical Union, 1998

Monsoons over the Arabian Sea---the oceanic basin that separates the Arabian peninsula from the I... more Monsoons over the Arabian Sea---the oceanic basin that separates the Arabian peninsula from the Indian subcontinent---follow seasonal cycles, reversing directions twice a year, in summer and winter. Rather than spreading across the expanse of the sea, the southwest (summer) monsoon is often concentrated into a jet over the central Arabian Sea. Evidence suggests that variations in wind stress force substantial

Research paper thumbnail of Dispersal of the Suwannee River plume over the West Florida shelf: Simulation and observation of the optical and biochemical consequences of a flushing event

Geophysical Research Letters, 2003