Paula Ruani - Academia.edu (original) (raw)

Papers by Paula Ruani

Research paper thumbnail of ATIVIDADE BIOLÓGICA DA Salacia impressifolia NA REGULAÇÃO DA GLICEMIA

Editora Reflexão Acadêmica eBooks, Nov 3, 2022

Research paper thumbnail of Myricitrin exhibits antidepressant-like effects and reduces IL-6 hippocampal levels in the chronic mild stress model

Behavioural Brain Research

Research paper thumbnail of The Symbiotic Fungus Leucoagaricus gongylophorus (Möller) Singer (Agaricales, Agaricaceae) as a Target Organism to Control Leaf-Cutting Ants

Insects, 2022

Atta and Acromyrmex are the main genera of leaf-cutting ants present in North and South America, ... more Atta and Acromyrmex are the main genera of leaf-cutting ants present in North and South America, causing extensive damage to agroforestry. Control of the ants requires high handling costs with few effective methods available to decrease the losses. The symbiosis between the leaf-cutting ants and the fungus Leucoagaricus gongylophorus is essential for ant nest survival. Therefore, L. gongylophorus may be a key target in controlling leaf-cutting ants, since its reduction may cause an imbalance in the symbiosis necessary to maintain the nest. Among the options for natural fungal control, plant species are considered important sources of compounds belonging to several classes of natural products that show potential as antifungal agents. This review also presents studies that establish that the antagonist fungi from the Escovopsis and Trichoderma genera effectively reduce the development of L. gongylophorus. The development of nanostructured delivery systems, which have shown advantages ...

Research paper thumbnail of Multiple-Injection Capillary Zone Electrophoresis as a Fast Strategy to Determine Antinitrosating Capacity of Commercial Teas

Journal of the Brazilian Chemical Society, 2018

Research paper thumbnail of Phytochemical and chemotaxonomic study of Polygala altomontana (Polygalaceae)

Biochemical Systematics and Ecology, 2018

In this study, 7 compounds were isolated from a hydroalcoholic extract of Polygala altomontana: a... more In this study, 7 compounds were isolated from a hydroalcoholic extract of Polygala altomontana: aurapten (1), a-spinasterol (2) dihydrostyryl-2-pyrone (3) and styryl-2-pyrones (4e7). The styryl-2-pyrone (5) is new in the literature and although the others are known compounds this is the first time they have been isolated from the species P. altomontana. This is the second species of the genus Polygala to present the accumulation of dihydrostyryl-2-pyrones and styryl-2-pyrones. The chemotaxonomic significance of this data for the Polygala genus was summarized.

Research paper thumbnail of A Rational Procedure to Increase Selectivity in the Determination of an Elicitor as Salicylic Acid in Polygala Extracts Using Capillary Electrophoresis

Journal of the Brazilian Chemical Society, 2021

Considering the economic potential of secondary metabolites, researches have been focusing severa... more Considering the economic potential of secondary metabolites, researches have been focusing several strategies in order to explore and enhance the accumulation of these metabolites in plant matrices in a broad context. One of those strategies is the development of suitable methodologies for the determination of some elicitors, such as salicylic acid (SA). Since literature presents only a few analytical methods for the quantification of SA in plants, this study developed, validated and evaluated these metabolite contents in Polygala species using capillary zone electrophoresis with ultraviolet-visible detection. The background electrolyte (BGE) consisted of 15 mmol L−1 of hydroxymethyl-aminomethane and 30 mmol L−1 of 2-hydroxy-isobutyric acid, pH = 3.9. The BGE was chosen using a rational procedure to increase selectivity and reduce migration time. The method presented rapid performance, migration time less than 0.76 min for salicylic and phthalic acid (internal standard), precision r...

Research paper thumbnail of Dihydrostyryl-2-pyrone as a chemical marker of three non-xanthone-producing Polygala species (Polygalaceae)

Biochemical Systematics and Ecology, 2020

Research paper thumbnail of A new xanthone as a chemical marker of four Polygala species (Polygalaceae)

Biochemical Systematics and Ecology, 2018

Research paper thumbnail of Pharmacological evidence favouring the traditional use of the root bark of Condalia buxifolia Reissek in the relief of pain and inflammation in mice

Journal of Ethnopharmacology, 2015

The Condalia buxifolia root bark infusion is used in traditional medicine in Brazil as antipyreti... more The Condalia buxifolia root bark infusion is used in traditional medicine in Brazil as antipyretic, anti-inflammatory and against dysentery. This study was designed to investigate whether the methanolic extract of the root bark of Condalia buxifolia (MECb) exhibits antinociceptive and anti-inflammatory effects in mice. Furthermore, also was investigated the involvement of glutamatergic and opioidergic system in the antinociceptive effect induced by MECb. The antinociceptive and anti-inflammatory effects of intra-gastric gavage (i.g.) administered MECb (10-300mg/kg) were evaluated in mice subjected to chemical (formalin, acetic-acid, glutamate) or thermal (hot plate) models of pain. The involvement of opioid system in the antinociceptive effect of the MECb was investigated in formalin test. Furthermore, a nonspecific effect of MECb was evaluated by measuring locomotor activity and exploratory behavior in open field test. Finally, was performed a phytochemical analysis of MECb. The phytochemical analysis of MECb was performed through HPLC analysis showing that the alkaloid Condaline-A is the main constituent. The intragastric administration of MECb (100-300mg/kg) significantly inhibited the nociception caused by acetic acid (48±2%), inflammatory phase (49±3%) and paw edema (32±6) caused by formalin, and MECb (100mg/kg, i.g.) also inhibited nociception caused by glutamate (41±7%). In addition, MECb (100-300mg/kg, i.g.) increased the paw withdrawal latency in hot-plate test, without affecting the locomotor activity and exploratory behavior in open field test. Finally, the antinociceptive effects of MECb (100mg/kg, i.g.) were significantly reversed by naloxone (1mg/kg, i.p.) in the formalin test. These data show, for the first time, that MECb has significant antinociceptive and anti-inflammatory effects, which appear to be related to the inhibition of the glutamatergic system and the activation of opioid mechanism, besides present central effects. These results support the use of Condalia buxifolia in traditional medicine and demonstrate that this plant has therapeutic potential for the development of phytomedicines with antinociceptive and anti-inflammatory properties.

Research paper thumbnail of Further antinociceptive effects of myricitrin in chemical models of overt nociception in mice

Neuroscience Letters, 2011

Research paper thumbnail of PAMPA Permeability, Acetylcholinesterase Inhibition and Antioxidant Activity of Pyranoisoflavones fromPolygala molluginifolia(Polygalaceae)

Journal of the Brazilian Chemical Society, 2013

Research paper thumbnail of P.2.c.005 Myricitrin, a PKC inhibitor, exerts antimanic-like effects in animal models

European Neuropsychopharmacology, 2013

S357 the SWN-K, for each point increase on the HAM-D. This strong inversely correlation is mainta... more S357 the SWN-K, for each point increase on the HAM-D. This strong inversely correlation is maintained between each subscales of the SWN-K and the HAM-D. There is a modest direct correlation (0.30 r 0.70, p < 0.01) between the total score on the YMRS with the total score on the SWN-K. Both variables are linearly related and this relationship direct, increasing an average of 0.94 points in the mental function subscale, for each point increase in the YMRS. In each subscale of the SWN-K is detected this relationship with the YMRS, and in all of them the relation is moderated and direct. Conclusions: According to previous studies, our results suggest that affective status is associated with subjective well-being in bipolar patients and this relationship is particular significant for depressive symptom. In contrast to previous studies, we found a modest but direct correlation between YMRS score and SWN-K, both in total score as in each subscale. It is likely that we could see changes in this relationship during follow-up, as Altamura et al. detected. Further investigations with larger sample sizes are necessary to assess subjective well-being in patients with acute mania in order to determine whether SWN-K is a good tool for it.

Research paper thumbnail of Mechanism of Action of Nutraceuticals on Intestine to Ameliorate Glucose Homeostasis: Follow-up Studies by an In Situ Approach

Journal of Cell Science & Therapy, 2014

Research paper thumbnail of ATIVIDADE BIOLÓGICA DA Salacia impressifolia NA REGULAÇÃO DA GLICEMIA

Editora Reflexão Acadêmica eBooks, Nov 3, 2022

Research paper thumbnail of Myricitrin exhibits antidepressant-like effects and reduces IL-6 hippocampal levels in the chronic mild stress model

Behavioural Brain Research

Research paper thumbnail of The Symbiotic Fungus Leucoagaricus gongylophorus (Möller) Singer (Agaricales, Agaricaceae) as a Target Organism to Control Leaf-Cutting Ants

Insects, 2022

Atta and Acromyrmex are the main genera of leaf-cutting ants present in North and South America, ... more Atta and Acromyrmex are the main genera of leaf-cutting ants present in North and South America, causing extensive damage to agroforestry. Control of the ants requires high handling costs with few effective methods available to decrease the losses. The symbiosis between the leaf-cutting ants and the fungus Leucoagaricus gongylophorus is essential for ant nest survival. Therefore, L. gongylophorus may be a key target in controlling leaf-cutting ants, since its reduction may cause an imbalance in the symbiosis necessary to maintain the nest. Among the options for natural fungal control, plant species are considered important sources of compounds belonging to several classes of natural products that show potential as antifungal agents. This review also presents studies that establish that the antagonist fungi from the Escovopsis and Trichoderma genera effectively reduce the development of L. gongylophorus. The development of nanostructured delivery systems, which have shown advantages ...

Research paper thumbnail of Multiple-Injection Capillary Zone Electrophoresis as a Fast Strategy to Determine Antinitrosating Capacity of Commercial Teas

Journal of the Brazilian Chemical Society, 2018

Research paper thumbnail of Phytochemical and chemotaxonomic study of Polygala altomontana (Polygalaceae)

Biochemical Systematics and Ecology, 2018

In this study, 7 compounds were isolated from a hydroalcoholic extract of Polygala altomontana: a... more In this study, 7 compounds were isolated from a hydroalcoholic extract of Polygala altomontana: aurapten (1), a-spinasterol (2) dihydrostyryl-2-pyrone (3) and styryl-2-pyrones (4e7). The styryl-2-pyrone (5) is new in the literature and although the others are known compounds this is the first time they have been isolated from the species P. altomontana. This is the second species of the genus Polygala to present the accumulation of dihydrostyryl-2-pyrones and styryl-2-pyrones. The chemotaxonomic significance of this data for the Polygala genus was summarized.

Research paper thumbnail of A Rational Procedure to Increase Selectivity in the Determination of an Elicitor as Salicylic Acid in Polygala Extracts Using Capillary Electrophoresis

Journal of the Brazilian Chemical Society, 2021

Considering the economic potential of secondary metabolites, researches have been focusing severa... more Considering the economic potential of secondary metabolites, researches have been focusing several strategies in order to explore and enhance the accumulation of these metabolites in plant matrices in a broad context. One of those strategies is the development of suitable methodologies for the determination of some elicitors, such as salicylic acid (SA). Since literature presents only a few analytical methods for the quantification of SA in plants, this study developed, validated and evaluated these metabolite contents in Polygala species using capillary zone electrophoresis with ultraviolet-visible detection. The background electrolyte (BGE) consisted of 15 mmol L−1 of hydroxymethyl-aminomethane and 30 mmol L−1 of 2-hydroxy-isobutyric acid, pH = 3.9. The BGE was chosen using a rational procedure to increase selectivity and reduce migration time. The method presented rapid performance, migration time less than 0.76 min for salicylic and phthalic acid (internal standard), precision r...

Research paper thumbnail of Dihydrostyryl-2-pyrone as a chemical marker of three non-xanthone-producing Polygala species (Polygalaceae)

Biochemical Systematics and Ecology, 2020

Research paper thumbnail of A new xanthone as a chemical marker of four Polygala species (Polygalaceae)

Biochemical Systematics and Ecology, 2018

Research paper thumbnail of Pharmacological evidence favouring the traditional use of the root bark of Condalia buxifolia Reissek in the relief of pain and inflammation in mice

Journal of Ethnopharmacology, 2015

The Condalia buxifolia root bark infusion is used in traditional medicine in Brazil as antipyreti... more The Condalia buxifolia root bark infusion is used in traditional medicine in Brazil as antipyretic, anti-inflammatory and against dysentery. This study was designed to investigate whether the methanolic extract of the root bark of Condalia buxifolia (MECb) exhibits antinociceptive and anti-inflammatory effects in mice. Furthermore, also was investigated the involvement of glutamatergic and opioidergic system in the antinociceptive effect induced by MECb. The antinociceptive and anti-inflammatory effects of intra-gastric gavage (i.g.) administered MECb (10-300mg/kg) were evaluated in mice subjected to chemical (formalin, acetic-acid, glutamate) or thermal (hot plate) models of pain. The involvement of opioid system in the antinociceptive effect of the MECb was investigated in formalin test. Furthermore, a nonspecific effect of MECb was evaluated by measuring locomotor activity and exploratory behavior in open field test. Finally, was performed a phytochemical analysis of MECb. The phytochemical analysis of MECb was performed through HPLC analysis showing that the alkaloid Condaline-A is the main constituent. The intragastric administration of MECb (100-300mg/kg) significantly inhibited the nociception caused by acetic acid (48±2%), inflammatory phase (49±3%) and paw edema (32±6) caused by formalin, and MECb (100mg/kg, i.g.) also inhibited nociception caused by glutamate (41±7%). In addition, MECb (100-300mg/kg, i.g.) increased the paw withdrawal latency in hot-plate test, without affecting the locomotor activity and exploratory behavior in open field test. Finally, the antinociceptive effects of MECb (100mg/kg, i.g.) were significantly reversed by naloxone (1mg/kg, i.p.) in the formalin test. These data show, for the first time, that MECb has significant antinociceptive and anti-inflammatory effects, which appear to be related to the inhibition of the glutamatergic system and the activation of opioid mechanism, besides present central effects. These results support the use of Condalia buxifolia in traditional medicine and demonstrate that this plant has therapeutic potential for the development of phytomedicines with antinociceptive and anti-inflammatory properties.

Research paper thumbnail of Further antinociceptive effects of myricitrin in chemical models of overt nociception in mice

Neuroscience Letters, 2011

Research paper thumbnail of PAMPA Permeability, Acetylcholinesterase Inhibition and Antioxidant Activity of Pyranoisoflavones fromPolygala molluginifolia(Polygalaceae)

Journal of the Brazilian Chemical Society, 2013

Research paper thumbnail of P.2.c.005 Myricitrin, a PKC inhibitor, exerts antimanic-like effects in animal models

European Neuropsychopharmacology, 2013

S357 the SWN-K, for each point increase on the HAM-D. This strong inversely correlation is mainta... more S357 the SWN-K, for each point increase on the HAM-D. This strong inversely correlation is maintained between each subscales of the SWN-K and the HAM-D. There is a modest direct correlation (0.30 r 0.70, p < 0.01) between the total score on the YMRS with the total score on the SWN-K. Both variables are linearly related and this relationship direct, increasing an average of 0.94 points in the mental function subscale, for each point increase in the YMRS. In each subscale of the SWN-K is detected this relationship with the YMRS, and in all of them the relation is moderated and direct. Conclusions: According to previous studies, our results suggest that affective status is associated with subjective well-being in bipolar patients and this relationship is particular significant for depressive symptom. In contrast to previous studies, we found a modest but direct correlation between YMRS score and SWN-K, both in total score as in each subscale. It is likely that we could see changes in this relationship during follow-up, as Altamura et al. detected. Further investigations with larger sample sizes are necessary to assess subjective well-being in patients with acute mania in order to determine whether SWN-K is a good tool for it.

Research paper thumbnail of Mechanism of Action of Nutraceuticals on Intestine to Ameliorate Glucose Homeostasis: Follow-up Studies by an In Situ Approach

Journal of Cell Science & Therapy, 2014