Paulo Cesar Ossani - Academia.edu (original) (raw)

Papers by Paulo Cesar Ossani

Animal reproduction, 2017

In bulls of the Nellore breed (Bos taurus indicus) it was observed four sperm cell subpopulations... more In bulls of the Nellore breed (Bos taurus indicus) it was observed four sperm cell subpopulations exhibiting asymmetrical distribution (Santos, et al. 2016. 18th International Congress on Animal Reproduction. Abstract book, Le Vinci in Tours, France, PW 913. p. 331-332). In this work we have investigated the relationship among these clusters and the fertility of semen samples, using in vitro fertilization rates. Nine straws were thawed in a water bath at 37° C for 30 seconds and fixed in buffered formal saline. Two hundred images of normal cells per animal were acquired by a CCD camera attached to a microscope and computer. The images were processed using tools of the software Image J, generating data on the morphometry of the sperm head. Multivariate analysis was performed using the Ward hierarchical clustering technique with Euclidean distance and determining the optimal number of groups by analysis of the level of distance and similarity behavior. Later, the k-means method was applied in the partition of each subpopulation. The Tukey test (P <0.05) was used to the validation of groups, in order to check the difference among variables averages. Until now, semen samples of nine animals were used for in vitro fertilization of matured oocytes from slaughterhouse ovaries. Twenty hours after fertilization, the potential zygotes were fixed in 4% paraformaldehyde, stained with Hoechst 33342 (5μg / ml) and pronuclear formation rate was evaluated by epifluorescence microscopy in order to determine the fertilization rate. Three replicates per animal were performed. The correlation among the proportion of sperm in each subpopulation and pronuclear formation rate was calculated. Statistical analysis was performed using the softwares Minitab 17.1.0 and R version 3.3.1, MVar.pt package version 1.9.4. Four different subpopulations were determined for all the evaluated bulls which differ in relation to the head area, with SP1 composed by the smaller cells (X = 966.00 ± 66.80 pix2 ), SP2 (X= 981.84 ± 69.27 pix2 ) and SP3 (X=1007.99 ± 82.91 pix2 ) by intermediate cells and SP4 composed by the larger head area sperm (X= 1075.51 ± 49.03 pix2 ). Six bulls out of nine animals evaluated showed a predominance of SP4 (X= 37.0% ± 5.6), two did not show this predominance (X=23.25% ± 10.25) and one animal presented homogeneous clusters distribution and no significant difference in the head area. For correlation analysis, in addition to the animal which did not differ by head area, a second animal was excluded because it was considered an outlier showing a high pronuclear formation rate (68.52%). A significant and negative correlation (P <0.05; r = -0.91) was found between the relative numbers of SP4 cells and pronuclear formation rate. These results showed that higher proportion of larger head area sperm cells in Bos taurus indicus ejaculates decreases semen in vitro fertility. It is possible that the presence and asymmetry of different morphometric sperm cell clusters in the bull could be determined by histones and the degree of nuclear protamination during spermiogenesis and epididymal transit, and also that cells with larger area have a lower degree of nuclear organization and lower fertility in relation to the other subpopulations. Currently a new approach is being conducted in order to investigate these possibilities. (CEUA/UFLA/Protocol#073/15).

Resumo: O café tem posição de destaque no cenário socioeconômico brasileiro, pois, além de ser re... more Resumo: O café tem posição de destaque no cenário socioeconômico brasileiro, pois, além de ser relevante na geração empregos e em toda economia, direta ou indiretamente, também é importante nas relações sociais. O estudo do comportamento dos consumidores é uma ferramenta importante para a identificação das motivações nos processos de compra do café nos diferentes segmentos desse mercado. Entretanto, ao considerar grupos de variáveis qualitativas em uma abordagem multivariada, as técnicas estatísticas usuais são limitadas ou inviáveis para descrever adequadamente o perfil sensorial dos consumidores. Este trabalho foi realizado com o objetivo de utilizar a técnica de Análise Múltiplos Fatores, no estudo do comportamento dos consumidores de café em uma pesquisa de opinião pública aplicada a um grupo de consumidores localizados na cidade de Varginha, MG. Foram usados seis assertivas (Likert de cinco pontos) relacionadas às percepções dos consumidores em relação à qualidade do café. Os resultados demonstram similaridade moderada nas respostas dadas pelos indivíduos nos subconjuntos das variáveis (marca e qualidade) e (preferência e preço), e baixa similaridade nos subconjuntos das variáveis (quantidade de xícaras de café consumidas), (torrefadora e produtor) e (região e produção). Concluiu-se que o uso desta técnica é viável, por permitir discriminar os comportamentos dos consumidores de cafés. Palavras-Chave: perfil sensorial, pesquisa de opinião, técnica MFA.

Revista da Universidade Vale do Rio Verde, 2017

In considering the study between groups of variables using a multivariate approach, the usual tec... more In considering the study between groups of variables using a multivariate approach, the usual techniques are either limited or unviable to describe how distinct these groups are. The multiple factor analysis technique (MFA) enables a statistical analysis among groups, whether they are quantitative, categorical, frequency or even mixed. The core of the technique is a factor analysis applied to the set of variables in which each group of variables is balanced, leading to a representation of the observations and variables. Thus, this package carries out the multiple factor analysis technique, generating easy results of being surveyed, as in a PCA (Principal Components Analysis), in addition to statistical plots of easy understanding. It possesses also some other functions for multivariate analysis, which are used by the MFA technique.

Bioscience Journal, Mar 17, 2020

The biotechnological interest in genus Physalis has increased in the last decades, however, there... more The biotechnological interest in genus Physalis has increased in the last decades, however, there are still few micropropagation studies of this genus. The objective of this study was to evaluate P. angulata photoautotrophic and photomixotrophic micropropagation with gas exchange under seven light spectra and five concentrations of sucrose. Lighting were yellow, blue, white, red, green, red + blue LEDs and natural light filtered by mesh. Sucrose concentrations were 0, 7.5, 15, 22.5 and 30 g.L-1. Phytotechnical, anatomical features and photopigment contents were evaluated through stem and root segment length, leaf number, leaf area, chlorophyll a and b contents, carotenoids, adaxial epidermis, palisadic and spongy parenchyma and abaxial epidermis. The data were compared by Scott-Knott's mean test and principal components analysis using the R software. Comparing the variables within lighting types, it was observed that only the screen treatment, screen-filtered natural illumination, obtained assessment in all variables. Comparing the levels of sucrose, it was observed that the treatment 15 g.L-1 sucrose obtained the highest number of averages with maximum evaluation. It was concluded that the natural light filtered by screen with 50% of shading allowed the photoautotrophic micropropagation of P. angulata. Better development results were observed in photomixotrophic micropropagation with 15 g.L-1 of sucrose.

Advances in biochemistry and biotechnology, 2019

Sperm morphometric subpopulations and hetero morphism in Bos taurus indicus bull semen and their ... more Sperm morphometric subpopulations and hetero morphism in Bos taurus indicus bull semen and their influence on in vitro fertilization were investigated. Area, center of mass and width obtained on 200 digitized cells/animal (n=9) were used to determine the subpopulations. The analysis of Ward's grouping with Euclidean distance was used to determine the subpopulations, and number of subpopulations was defined according to the behavior of distance level and similarity. Fertility was evaluated by Pro-Nuclei Formation Rate (PFR) after In Vitro Fertilization (IVF). Sperm hetero morphism in one hundred cells considered to be morphologically normal was assessed by Harmonic Fourier Descriptors and Principal Component Analysis (PCA). Four subpopulations (SP1 to SP4) were determined, exhibiting asymmetrical distribution. SP4 was composed by 43.0% of the cells that showed the greatest area (39.3 ± 1.8 µm 2), while SP1 (35.3±2.4 µm 2), SP2 (35.9 ± 2.5 µm 2), and SP3 (36.9 ± 3.0 µm 2) presented similar distribution (21.0%; 17.5%; 18.5%; respectively). A negative correlation (r=-0.86; P=0.01) was observed between the SP4 and PFR. Hetero morphism rate did not influence PFR. In conclusion, semen of Bos taurus indicus bulls exhibited morphological heterogeneous structure and cells exhibiting large head area presented lower capacity of IVF.

Brazilian Journal of Food Technology

The use of unconventional edible plants in human food has grown more widespread in recent years, ... more The use of unconventional edible plants in human food has grown more widespread in recent years, driven mainly by gourmet dishes. Evidence of the food properties of these species is still scarce. Aiming to obtain more in-depth knowledge of dahlia plants, this study aimed to evaluate the nutritional, bioactive, and antinutritional properties of the edible parts of purple dahlia (tuberous root and flower) to elucidate their value and safety as foods. The percent composition, caloric value, pH, Total Soluble Solids (TSS), Total Titratable Acidity (TTA), ratio of TSS to TTA, anthocyanins, carotenoids, vitamin C, total phenolics, tannins, nitrates, and minerals of the roots and flowers were analyzed. The inulin content in the root and the color of the flower were also evaluated. The results were reported as mean and standard deviation, and exploratory factor analysis was performed. Both parts of the dahlia had constituents that contribute to a good diet at concentrations like those in co...

Industrial Crops and Products

Resumo: Com o intuito de desenvolver cultivares de morangueiro adaptadas as condicoes edafoclimat... more Resumo: Com o intuito de desenvolver cultivares de morangueiro adaptadas as condicoes edafoclimaticas brasileiras sao avaliados e selecionados genitores que apresentem caracteristicas geneticas promissoras. Durante a selecao de materiais algumas estrategias tem sido adotadas visando o sucesso do programa de melhoramento genetico, a exemplo, o uso de ferramentas estatisticas como as tecnicas multivariadas de analise de dados. Objetivou-se com esse trabalho avaliar o nivel de similaridade entre genotipos de morangueiro com o proposito de diagnosticar possiveis agrupamentos em relacao as variaveis analisadas, utilizando as tecnicas de analises multivariadas. Foram estudados 103 hibridos experimentais desenvolvidos pelo programa de melhoramento genetico da Universidade Federal de Lavras (UFLA) e sete cultivares comerciais importadas, avaliando as caracteristicas de producao e as variaveis fisico-quimicas do fruto de cada genotipo, utilizando-se a analise de multiplos fatores (MFA) e de ...

Journal of Seed Science, 2018

The accelerated aging test is recognized as an efficient method for evaluating the vigor of seed ... more The accelerated aging test is recognized as an efficient method for evaluating the vigor of seed lots and for estimating their storage potential. Thus, this work aimed to evaluate the association between artificial aging and natural storage of coffee seeds, through the correlation factor analysis. Seeds of four cultivars of Coffea arabica L. (Catuaí Amarelo, Arara, Catiguá, and Mundo Novo) and one of Coffea canephora Pierre (Apoatã) were used. Part of the newly-harvested seeds were aged in a growth chamber under controlled temperature and relative humidity conditions (42 ºC and 100% RH) for periods of 0, 4, 6, 8, and 10 days. The other part of the seeds was stored in tri-wall paper packaging for a period of 2, 4, and 6 months in a non-climate-controlled environment. Artificial aging allows predictions on the storage potential of coffee seeds, although the artificial aging periods depend on the cultivars.

Os diferentes indices de mercado sao criados na tentativa de apresentar uma tendencia para a medi... more Os diferentes indices de mercado sao criados na tentativa de apresentar uma tendencia para a media de negociacoes. O processo de indexacao muitas vezes resulta na valorizacao das empresas incluidas ou desvalorizacao das excluidas. Hipoteses que explicam a ocorrencia desses eventos remetem a negociacao de fundos de indice ou mesmo a criacao de um interesse diferenciado pela divulgacao das novas composicoes. Desta forma, o objetivo e avaliar os impactos da alteracao da composicao da carteira teorica do Indice Bovespa – IBOVESPA, divulgada entre abril e maio de 2015. Buscou-se a observacao de alguma intervencao significativa associada aos momentos da mudanca desta carteira, relacionados as duas previas e ao anuncio da efetiva alteracao. Para mensuracao do efeito indice foi utilizada a analise de intervencao. Foram definidos eventos associados a mudanca. As series foram corrigidas para eliminar tendencia e sazonalidade. A amostra foi composta de uma inclusao e tres exclusoes. Observaram...

Ciência Animal Brasileira

The objective of this study was to characterize calvings with low and high difficulty based on th... more The objective of this study was to characterize calvings with low and high difficulty based on the productive and reproductive performance of dairy cows. Calvings were grouped in no calving assistance, calving with low assistance, and calving with high assistance. The original data set comprised 1,902 calving records obtained from a large dairy farm in Southeast Brazil. Factor analysis was applied using the SAS® Studio 3.8 statistical program through the factor procedure, considering the Multivariate Analysis category. Milk fat (0.92–0.79) and total solids (0.91–0.80) were strongly correlated with Factor 1. Calving interval (0.87– 0.68) and the number of AI (artificial inseminations) per conception (0.87–0.71) showed high correlations with Factor 2. Milk yield (0.84–0.76) and accumulated milk yield (0.84–0.77) were strongly correlated with Factor 3. Based on the results, we conclude that the three calving scenarios were characterized by well-defined and independent factors. Cows whi...

Ciência Animal Brasileira

Resumo O objetivo deste estudo foi caracterizar os partos com leve ou severa dificuldade e difere... more Resumo O objetivo deste estudo foi caracterizar os partos com leve ou severa dificuldade e diferenciá-los com base no desempenho produtivo e reprodutivo de vacas leiteiras. Os partos foram agrupados em partos sem assistência, partos com baixa assistência e partos com elevada assistência. O banco de dados original continha 1902 registro de partos que foram obtidos de uma grande fazenda comercial localizada no Sudeste do Brasil. A análise fatorial foi aplicada através do programa estatístico SAS® Studio 3.8 por meio de procedimento fatorial, considerando a categoria de análise multivariada. Os teores de gordura do leite (0,92– 0,79) e de sólidos totais (0,91–0,80) foram altamente correlacionados com o fator 1. Intervalo entre partos (0,87–0,68) e número de IA (inseminações artificiais) por concepção (0,87–0,71) apresentaram alta correlação com o fator 2. Produção de leite (0,84–0,76) e produção acumulada de leite (0,84–0,77) foram altamente correlacionados com o fator 3. Baseados nos ...

Horticultura Brasileira

The tomato is one of the most economically, socially and nutritionally important vegetables, pres... more The tomato is one of the most economically, socially and nutritionally important vegetables, presenting high added value. The culture allows high profit for the producers, but it is a high risk activity due to, specially, the sensibility of some varieties to climate conditions as well as multiple plagues and diseases that attack the culture. Therefore, the search for new hybrids with more tolerance to biotic and abiotic factors is extremely important to attend to the demand of the culture’s productive chain. The objective of this work was to demonstrate the importance of multivariate and univariate techniques in evaluating the level of similarity among hybrids of the tomato plant of the salad group with the purpose of diagnosing possible groups in relation to the variables analyzed. A total of 23 hybrids of tomato from the salad group were evaluated; of those, 9 were commercial hybrids and 14 were experimental hybrids. Throughout the conduction of the experiment, the productive char...

Revista Agroecossistemas, 2020

A carência de conhecimento no que diz respeito ao ciclo da araruta, pode acarretar em prejuízos n... more A carência de conhecimento no que diz respeito ao ciclo da araruta, pode acarretar em prejuízos na produção, pois a colheita antecipada pode ocasionar perdas por não atingir o nível máximo de acúmulo de matéria seca nos rizomas e a colheita tardia aumenta o índice de podridão dos rizomas, diminuindo a qualidade do mesmo. Objetivou-se com este trabalho, determinar o efeito da época de colheita na produtividade e na qualidade química de amido de araruta. Foram utilizados rizomas de araruta, colhidos em cinco épocas diferentes (253, 273, 295, 314 e 336 dias após o plantio). No campo o experimento foi instalado em setembro de 2016 a agosto de 2017 no município de Lavras-MG em delineamento em blocos casualizados, com três repetições. No laboratório os experimentos foram realizados em delineamento inteiramente casualizados com três repetições, foi avaliado o rendimento da extração, composição centesimal, quantidade de amido purificado e análise de microscopia eletrônica. Os efeitos quanti...

Research, Society and Development

Specialty coffees have a big importance in the economic scenario, and its sensory quality is appr... more Specialty coffees have a big importance in the economic scenario, and its sensory quality is appreciated by the productive sector and by the market. Researches have been constantly carried out in the search for better blends in order to add value and differentiate prices according to the product quality. To accomplish that, new methodologies must be explored, taking into consideration factors that might differentiate the particularities of each consumer and/or product. Thus, this article suggests the use of the machine learning technique in the construction of supervised classification and identification models. In a sensory evaluation test for consumer acceptance using four classes of specialty coffees, applied to four groups of trained and untrained consumers, features such as flavor, body, sweetness and general grade were evaluated. The use of machine learning is viable because it allows the classification and identification of specialty coffees produced in different altitudes an...

Pesquisa Agropecuária Brasileira

The objective of this work was to carry out the morphological characterization of strawberry frui... more The objective of this work was to carry out the morphological characterization of strawberry fruit of different genotypes, as well as to verify their postharvest conservation. Six commercial cultivars (Albion, Aromas, Dover, Festival, Pircinque, and San Andreas) and nine experimental clones (MDA01, MDA19, MDA22, MDA23, MCA89, MCA94, MFA443, MOGSC468, and MFA443PR) were evaluated. Fruit were produced on semi-hydroponic gutters in protected cultivation and classified according to size, shape, color, brightness, achene position, pulp color, “heart” color, fruit cavity, and sepal position. For the postharvest analysis of fruit, the loss of mass and the appearance of fruit during ten days of storage were determined. The evaluated genotypes show a high similarity to each other regarding morphological characterization. MCA89 and 'Pircinque' are the most divergent genotypes in relation to the others. There is an association between the characteristics large cavity, reniform shape, a...

Research, Society and Development, 2021

Specialty coffees have a big importance in the economic scenario, and its sensory quality is appr... more Specialty coffees have a big importance in the economic scenario, and its sensory quality is appreciated by the productive sector and by the market. Researches have been constantly carried out in the search for better blends in order to add value and differentiate prices according to the product quality. To accomplish that, new methodologies must be explored, taking into consideration factors that might differentiate the particularities of each consumer and/or product. Thus, this article suggests the use of the machine learning technique in the construction of supervised classification and identification models. In a sensory evaluation test for consumer acceptance using four classes of specialty coffees, applied to four groups of trained and untrained consumers, features such as flavor, body, sweetness and general grade were evaluated. The use of machine learning is viable because it allows the classification and identification of specialty coffees produced in different altitudes an...

A carencia de conhecimento no que diz respeito ao ciclo da araruta, pode acarretar em prejuizos n... more A carencia de conhecimento no que diz respeito ao ciclo da araruta, pode acarretar em prejuizos na producao, pois a colheita antecipada pode ocasionar perdas por nao atingir o nivel maximo de acumulo de materia seca nos rizomas e a colheita tardia aumenta o indice de podridao dos rizomas, diminuindo a qualidade do mesmo. Objetivou-se com este trabalho, determinar o efeito da epoca de colheita na produtividade e na qualidade quimica de amido de araruta. Foram utilizados rizomas de araruta, colhidos em cinco epocas diferentes (253, 273, 295, 314 e 336 dias apos o plantio). No campo o experimento foi instalado em setembro de 2016 a agosto de 2017 no municipio de Lavras-MG em delineamento em blocos casualizados, com tres repeticoes. No laboratorio os experimentos foram realizados em delineamento inteiramente casualizados com tres repeticoes, foi avaliado o rendimento da extracao, composicao centesimal, quantidade de amido purificado e analise de microscopia eletronica. Os efeitos quanti...

Animal reproduction, 2017

In bulls of the Nellore breed (Bos taurus indicus) it was observed four sperm cell subpopulations... more In bulls of the Nellore breed (Bos taurus indicus) it was observed four sperm cell subpopulations exhibiting asymmetrical distribution (Santos, et al. 2016. 18th International Congress on Animal Reproduction. Abstract book, Le Vinci in Tours, France, PW 913. p. 331-332). In this work we have investigated the relationship among these clusters and the fertility of semen samples, using in vitro fertilization rates. Nine straws were thawed in a water bath at 37° C for 30 seconds and fixed in buffered formal saline. Two hundred images of normal cells per animal were acquired by a CCD camera attached to a microscope and computer. The images were processed using tools of the software Image J, generating data on the morphometry of the sperm head. Multivariate analysis was performed using the Ward hierarchical clustering technique with Euclidean distance and determining the optimal number of groups by analysis of the level of distance and similarity behavior. Later, the k-means method was applied in the partition of each subpopulation. The Tukey test (P <0.05) was used to the validation of groups, in order to check the difference among variables averages. Until now, semen samples of nine animals were used for in vitro fertilization of matured oocytes from slaughterhouse ovaries. Twenty hours after fertilization, the potential zygotes were fixed in 4% paraformaldehyde, stained with Hoechst 33342 (5μg / ml) and pronuclear formation rate was evaluated by epifluorescence microscopy in order to determine the fertilization rate. Three replicates per animal were performed. The correlation among the proportion of sperm in each subpopulation and pronuclear formation rate was calculated. Statistical analysis was performed using the softwares Minitab 17.1.0 and R version 3.3.1, MVar.pt package version 1.9.4. Four different subpopulations were determined for all the evaluated bulls which differ in relation to the head area, with SP1 composed by the smaller cells (X = 966.00 ± 66.80 pix2 ), SP2 (X= 981.84 ± 69.27 pix2 ) and SP3 (X=1007.99 ± 82.91 pix2 ) by intermediate cells and SP4 composed by the larger head area sperm (X= 1075.51 ± 49.03 pix2 ). Six bulls out of nine animals evaluated showed a predominance of SP4 (X= 37.0% ± 5.6), two did not show this predominance (X=23.25% ± 10.25) and one animal presented homogeneous clusters distribution and no significant difference in the head area. For correlation analysis, in addition to the animal which did not differ by head area, a second animal was excluded because it was considered an outlier showing a high pronuclear formation rate (68.52%). A significant and negative correlation (P <0.05; r = -0.91) was found between the relative numbers of SP4 cells and pronuclear formation rate. These results showed that higher proportion of larger head area sperm cells in Bos taurus indicus ejaculates decreases semen in vitro fertility. It is possible that the presence and asymmetry of different morphometric sperm cell clusters in the bull could be determined by histones and the degree of nuclear protamination during spermiogenesis and epididymal transit, and also that cells with larger area have a lower degree of nuclear organization and lower fertility in relation to the other subpopulations. Currently a new approach is being conducted in order to investigate these possibilities. (CEUA/UFLA/Protocol#073/15).

Resumo: O café tem posição de destaque no cenário socioeconômico brasileiro, pois, além de ser re... more Resumo: O café tem posição de destaque no cenário socioeconômico brasileiro, pois, além de ser relevante na geração empregos e em toda economia, direta ou indiretamente, também é importante nas relações sociais. O estudo do comportamento dos consumidores é uma ferramenta importante para a identificação das motivações nos processos de compra do café nos diferentes segmentos desse mercado. Entretanto, ao considerar grupos de variáveis qualitativas em uma abordagem multivariada, as técnicas estatísticas usuais são limitadas ou inviáveis para descrever adequadamente o perfil sensorial dos consumidores. Este trabalho foi realizado com o objetivo de utilizar a técnica de Análise Múltiplos Fatores, no estudo do comportamento dos consumidores de café em uma pesquisa de opinião pública aplicada a um grupo de consumidores localizados na cidade de Varginha, MG. Foram usados seis assertivas (Likert de cinco pontos) relacionadas às percepções dos consumidores em relação à qualidade do café. Os resultados demonstram similaridade moderada nas respostas dadas pelos indivíduos nos subconjuntos das variáveis (marca e qualidade) e (preferência e preço), e baixa similaridade nos subconjuntos das variáveis (quantidade de xícaras de café consumidas), (torrefadora e produtor) e (região e produção). Concluiu-se que o uso desta técnica é viável, por permitir discriminar os comportamentos dos consumidores de cafés. Palavras-Chave: perfil sensorial, pesquisa de opinião, técnica MFA.

Revista da Universidade Vale do Rio Verde, 2017

In considering the study between groups of variables using a multivariate approach, the usual tec... more In considering the study between groups of variables using a multivariate approach, the usual techniques are either limited or unviable to describe how distinct these groups are. The multiple factor analysis technique (MFA) enables a statistical analysis among groups, whether they are quantitative, categorical, frequency or even mixed. The core of the technique is a factor analysis applied to the set of variables in which each group of variables is balanced, leading to a representation of the observations and variables. Thus, this package carries out the multiple factor analysis technique, generating easy results of being surveyed, as in a PCA (Principal Components Analysis), in addition to statistical plots of easy understanding. It possesses also some other functions for multivariate analysis, which are used by the MFA technique.

Bioscience Journal, Mar 17, 2020

The biotechnological interest in genus Physalis has increased in the last decades, however, there... more The biotechnological interest in genus Physalis has increased in the last decades, however, there are still few micropropagation studies of this genus. The objective of this study was to evaluate P. angulata photoautotrophic and photomixotrophic micropropagation with gas exchange under seven light spectra and five concentrations of sucrose. Lighting were yellow, blue, white, red, green, red + blue LEDs and natural light filtered by mesh. Sucrose concentrations were 0, 7.5, 15, 22.5 and 30 g.L-1. Phytotechnical, anatomical features and photopigment contents were evaluated through stem and root segment length, leaf number, leaf area, chlorophyll a and b contents, carotenoids, adaxial epidermis, palisadic and spongy parenchyma and abaxial epidermis. The data were compared by Scott-Knott's mean test and principal components analysis using the R software. Comparing the variables within lighting types, it was observed that only the screen treatment, screen-filtered natural illumination, obtained assessment in all variables. Comparing the levels of sucrose, it was observed that the treatment 15 g.L-1 sucrose obtained the highest number of averages with maximum evaluation. It was concluded that the natural light filtered by screen with 50% of shading allowed the photoautotrophic micropropagation of P. angulata. Better development results were observed in photomixotrophic micropropagation with 15 g.L-1 of sucrose.

Advances in biochemistry and biotechnology, 2019

Sperm morphometric subpopulations and hetero morphism in Bos taurus indicus bull semen and their ... more Sperm morphometric subpopulations and hetero morphism in Bos taurus indicus bull semen and their influence on in vitro fertilization were investigated. Area, center of mass and width obtained on 200 digitized cells/animal (n=9) were used to determine the subpopulations. The analysis of Ward's grouping with Euclidean distance was used to determine the subpopulations, and number of subpopulations was defined according to the behavior of distance level and similarity. Fertility was evaluated by Pro-Nuclei Formation Rate (PFR) after In Vitro Fertilization (IVF). Sperm hetero morphism in one hundred cells considered to be morphologically normal was assessed by Harmonic Fourier Descriptors and Principal Component Analysis (PCA). Four subpopulations (SP1 to SP4) were determined, exhibiting asymmetrical distribution. SP4 was composed by 43.0% of the cells that showed the greatest area (39.3 ± 1.8 µm 2), while SP1 (35.3±2.4 µm 2), SP2 (35.9 ± 2.5 µm 2), and SP3 (36.9 ± 3.0 µm 2) presented similar distribution (21.0%; 17.5%; 18.5%; respectively). A negative correlation (r=-0.86; P=0.01) was observed between the SP4 and PFR. Hetero morphism rate did not influence PFR. In conclusion, semen of Bos taurus indicus bulls exhibited morphological heterogeneous structure and cells exhibiting large head area presented lower capacity of IVF.

Brazilian Journal of Food Technology

The use of unconventional edible plants in human food has grown more widespread in recent years, ... more The use of unconventional edible plants in human food has grown more widespread in recent years, driven mainly by gourmet dishes. Evidence of the food properties of these species is still scarce. Aiming to obtain more in-depth knowledge of dahlia plants, this study aimed to evaluate the nutritional, bioactive, and antinutritional properties of the edible parts of purple dahlia (tuberous root and flower) to elucidate their value and safety as foods. The percent composition, caloric value, pH, Total Soluble Solids (TSS), Total Titratable Acidity (TTA), ratio of TSS to TTA, anthocyanins, carotenoids, vitamin C, total phenolics, tannins, nitrates, and minerals of the roots and flowers were analyzed. The inulin content in the root and the color of the flower were also evaluated. The results were reported as mean and standard deviation, and exploratory factor analysis was performed. Both parts of the dahlia had constituents that contribute to a good diet at concentrations like those in co...

Industrial Crops and Products

Resumo: Com o intuito de desenvolver cultivares de morangueiro adaptadas as condicoes edafoclimat... more Resumo: Com o intuito de desenvolver cultivares de morangueiro adaptadas as condicoes edafoclimaticas brasileiras sao avaliados e selecionados genitores que apresentem caracteristicas geneticas promissoras. Durante a selecao de materiais algumas estrategias tem sido adotadas visando o sucesso do programa de melhoramento genetico, a exemplo, o uso de ferramentas estatisticas como as tecnicas multivariadas de analise de dados. Objetivou-se com esse trabalho avaliar o nivel de similaridade entre genotipos de morangueiro com o proposito de diagnosticar possiveis agrupamentos em relacao as variaveis analisadas, utilizando as tecnicas de analises multivariadas. Foram estudados 103 hibridos experimentais desenvolvidos pelo programa de melhoramento genetico da Universidade Federal de Lavras (UFLA) e sete cultivares comerciais importadas, avaliando as caracteristicas de producao e as variaveis fisico-quimicas do fruto de cada genotipo, utilizando-se a analise de multiplos fatores (MFA) e de ...

Journal of Seed Science, 2018

The accelerated aging test is recognized as an efficient method for evaluating the vigor of seed ... more The accelerated aging test is recognized as an efficient method for evaluating the vigor of seed lots and for estimating their storage potential. Thus, this work aimed to evaluate the association between artificial aging and natural storage of coffee seeds, through the correlation factor analysis. Seeds of four cultivars of Coffea arabica L. (Catuaí Amarelo, Arara, Catiguá, and Mundo Novo) and one of Coffea canephora Pierre (Apoatã) were used. Part of the newly-harvested seeds were aged in a growth chamber under controlled temperature and relative humidity conditions (42 ºC and 100% RH) for periods of 0, 4, 6, 8, and 10 days. The other part of the seeds was stored in tri-wall paper packaging for a period of 2, 4, and 6 months in a non-climate-controlled environment. Artificial aging allows predictions on the storage potential of coffee seeds, although the artificial aging periods depend on the cultivars.

Os diferentes indices de mercado sao criados na tentativa de apresentar uma tendencia para a medi... more Os diferentes indices de mercado sao criados na tentativa de apresentar uma tendencia para a media de negociacoes. O processo de indexacao muitas vezes resulta na valorizacao das empresas incluidas ou desvalorizacao das excluidas. Hipoteses que explicam a ocorrencia desses eventos remetem a negociacao de fundos de indice ou mesmo a criacao de um interesse diferenciado pela divulgacao das novas composicoes. Desta forma, o objetivo e avaliar os impactos da alteracao da composicao da carteira teorica do Indice Bovespa – IBOVESPA, divulgada entre abril e maio de 2015. Buscou-se a observacao de alguma intervencao significativa associada aos momentos da mudanca desta carteira, relacionados as duas previas e ao anuncio da efetiva alteracao. Para mensuracao do efeito indice foi utilizada a analise de intervencao. Foram definidos eventos associados a mudanca. As series foram corrigidas para eliminar tendencia e sazonalidade. A amostra foi composta de uma inclusao e tres exclusoes. Observaram...

Ciência Animal Brasileira

The objective of this study was to characterize calvings with low and high difficulty based on th... more The objective of this study was to characterize calvings with low and high difficulty based on the productive and reproductive performance of dairy cows. Calvings were grouped in no calving assistance, calving with low assistance, and calving with high assistance. The original data set comprised 1,902 calving records obtained from a large dairy farm in Southeast Brazil. Factor analysis was applied using the SAS® Studio 3.8 statistical program through the factor procedure, considering the Multivariate Analysis category. Milk fat (0.92–0.79) and total solids (0.91–0.80) were strongly correlated with Factor 1. Calving interval (0.87– 0.68) and the number of AI (artificial inseminations) per conception (0.87–0.71) showed high correlations with Factor 2. Milk yield (0.84–0.76) and accumulated milk yield (0.84–0.77) were strongly correlated with Factor 3. Based on the results, we conclude that the three calving scenarios were characterized by well-defined and independent factors. Cows whi...

Ciência Animal Brasileira

Resumo O objetivo deste estudo foi caracterizar os partos com leve ou severa dificuldade e difere... more Resumo O objetivo deste estudo foi caracterizar os partos com leve ou severa dificuldade e diferenciá-los com base no desempenho produtivo e reprodutivo de vacas leiteiras. Os partos foram agrupados em partos sem assistência, partos com baixa assistência e partos com elevada assistência. O banco de dados original continha 1902 registro de partos que foram obtidos de uma grande fazenda comercial localizada no Sudeste do Brasil. A análise fatorial foi aplicada através do programa estatístico SAS® Studio 3.8 por meio de procedimento fatorial, considerando a categoria de análise multivariada. Os teores de gordura do leite (0,92– 0,79) e de sólidos totais (0,91–0,80) foram altamente correlacionados com o fator 1. Intervalo entre partos (0,87–0,68) e número de IA (inseminações artificiais) por concepção (0,87–0,71) apresentaram alta correlação com o fator 2. Produção de leite (0,84–0,76) e produção acumulada de leite (0,84–0,77) foram altamente correlacionados com o fator 3. Baseados nos ...

Horticultura Brasileira

The tomato is one of the most economically, socially and nutritionally important vegetables, pres... more The tomato is one of the most economically, socially and nutritionally important vegetables, presenting high added value. The culture allows high profit for the producers, but it is a high risk activity due to, specially, the sensibility of some varieties to climate conditions as well as multiple plagues and diseases that attack the culture. Therefore, the search for new hybrids with more tolerance to biotic and abiotic factors is extremely important to attend to the demand of the culture’s productive chain. The objective of this work was to demonstrate the importance of multivariate and univariate techniques in evaluating the level of similarity among hybrids of the tomato plant of the salad group with the purpose of diagnosing possible groups in relation to the variables analyzed. A total of 23 hybrids of tomato from the salad group were evaluated; of those, 9 were commercial hybrids and 14 were experimental hybrids. Throughout the conduction of the experiment, the productive char...

Revista Agroecossistemas, 2020

A carência de conhecimento no que diz respeito ao ciclo da araruta, pode acarretar em prejuízos n... more A carência de conhecimento no que diz respeito ao ciclo da araruta, pode acarretar em prejuízos na produção, pois a colheita antecipada pode ocasionar perdas por não atingir o nível máximo de acúmulo de matéria seca nos rizomas e a colheita tardia aumenta o índice de podridão dos rizomas, diminuindo a qualidade do mesmo. Objetivou-se com este trabalho, determinar o efeito da época de colheita na produtividade e na qualidade química de amido de araruta. Foram utilizados rizomas de araruta, colhidos em cinco épocas diferentes (253, 273, 295, 314 e 336 dias após o plantio). No campo o experimento foi instalado em setembro de 2016 a agosto de 2017 no município de Lavras-MG em delineamento em blocos casualizados, com três repetições. No laboratório os experimentos foram realizados em delineamento inteiramente casualizados com três repetições, foi avaliado o rendimento da extração, composição centesimal, quantidade de amido purificado e análise de microscopia eletrônica. Os efeitos quanti...

Research, Society and Development

Specialty coffees have a big importance in the economic scenario, and its sensory quality is appr... more Specialty coffees have a big importance in the economic scenario, and its sensory quality is appreciated by the productive sector and by the market. Researches have been constantly carried out in the search for better blends in order to add value and differentiate prices according to the product quality. To accomplish that, new methodologies must be explored, taking into consideration factors that might differentiate the particularities of each consumer and/or product. Thus, this article suggests the use of the machine learning technique in the construction of supervised classification and identification models. In a sensory evaluation test for consumer acceptance using four classes of specialty coffees, applied to four groups of trained and untrained consumers, features such as flavor, body, sweetness and general grade were evaluated. The use of machine learning is viable because it allows the classification and identification of specialty coffees produced in different altitudes an...

Pesquisa Agropecuária Brasileira

The objective of this work was to carry out the morphological characterization of strawberry frui... more The objective of this work was to carry out the morphological characterization of strawberry fruit of different genotypes, as well as to verify their postharvest conservation. Six commercial cultivars (Albion, Aromas, Dover, Festival, Pircinque, and San Andreas) and nine experimental clones (MDA01, MDA19, MDA22, MDA23, MCA89, MCA94, MFA443, MOGSC468, and MFA443PR) were evaluated. Fruit were produced on semi-hydroponic gutters in protected cultivation and classified according to size, shape, color, brightness, achene position, pulp color, “heart” color, fruit cavity, and sepal position. For the postharvest analysis of fruit, the loss of mass and the appearance of fruit during ten days of storage were determined. The evaluated genotypes show a high similarity to each other regarding morphological characterization. MCA89 and 'Pircinque' are the most divergent genotypes in relation to the others. There is an association between the characteristics large cavity, reniform shape, a...

Research, Society and Development, 2021

Specialty coffees have a big importance in the economic scenario, and its sensory quality is appr... more Specialty coffees have a big importance in the economic scenario, and its sensory quality is appreciated by the productive sector and by the market. Researches have been constantly carried out in the search for better blends in order to add value and differentiate prices according to the product quality. To accomplish that, new methodologies must be explored, taking into consideration factors that might differentiate the particularities of each consumer and/or product. Thus, this article suggests the use of the machine learning technique in the construction of supervised classification and identification models. In a sensory evaluation test for consumer acceptance using four classes of specialty coffees, applied to four groups of trained and untrained consumers, features such as flavor, body, sweetness and general grade were evaluated. The use of machine learning is viable because it allows the classification and identification of specialty coffees produced in different altitudes an...

A carencia de conhecimento no que diz respeito ao ciclo da araruta, pode acarretar em prejuizos n... more A carencia de conhecimento no que diz respeito ao ciclo da araruta, pode acarretar em prejuizos na producao, pois a colheita antecipada pode ocasionar perdas por nao atingir o nivel maximo de acumulo de materia seca nos rizomas e a colheita tardia aumenta o indice de podridao dos rizomas, diminuindo a qualidade do mesmo. Objetivou-se com este trabalho, determinar o efeito da epoca de colheita na produtividade e na qualidade quimica de amido de araruta. Foram utilizados rizomas de araruta, colhidos em cinco epocas diferentes (253, 273, 295, 314 e 336 dias apos o plantio). No campo o experimento foi instalado em setembro de 2016 a agosto de 2017 no municipio de Lavras-MG em delineamento em blocos casualizados, com tres repeticoes. No laboratorio os experimentos foram realizados em delineamento inteiramente casualizados com tres repeticoes, foi avaliado o rendimento da extracao, composicao centesimal, quantidade de amido purificado e analise de microscopia eletronica. Os efeitos quanti...