Paulo Ciarlini - Academia.edu (original) (raw)
Papers by Paulo Ciarlini
Ars Veterinaria, 2008
It was evaluated the influence of sex and age in proteinogram from newborn and young ostriches ra... more It was evaluated the influence of sex and age in proteinogram from newborn and young ostriches raised round Aracatuba, Sao Paulo. The total protein and its fractions in plasma samples were determined from 45 ostriches divided into three groups: 1-|15 days, 15-|30 days and 30-|70 days old. The total protein was measured using refractometric method and fractions were determined by electrophoresis on cellulose acetate membranes. There were not significant differences between males and females in values obtained for total protein in plasma (PPT), albumin, a , b , g -globulins. In ostriches until 15 days old, the PPT and fractions were higher (p<0,05) than those of ostriches with more 30 days old. The PPT and g -globulin of youger ostriches were higher (p<0,05) than those intermediate age. The results showed the metabolism of proteins in ostriches suffer significant changes in the first 70 days of age. KEY-WORDS : Ratite. Electrophoresis. Total protein in plasma. Age. Sex.
Ciencia Rural, Jul 24, 2009
Metabolismo oxidativo de heterófilos de avestruzes jovens.
Ciencia Rural, Jul 24, 2009
Metabolismo oxidativo de heterófilos de avestruzes jovens.
Revista Brasileira De Parasitologia Veterinaria, 2000
Revista Brasileira De Parasitologia Veterinaria, 2000
Research in Veterinary Science, May 1, 2021
Oxidative stress (OS) has been strongly associated with postprandial lipemia (PPL) in humans, and... more Oxidative stress (OS) has been strongly associated with postprandial lipemia (PPL) in humans, and still requires further investigation in dogs. However, since lipemia interferes with spectrophotometric determinations such as those used to assess OS, the present study investigated the effect of PPL on OS parameters of healthy dogs. Twenty dogs had lipemic postprandial samples compared to the average of two non-lipemic moments. Subsequently, PPL was simulated in vitro using a commercial lipid emulsion and twelve pools of non-lipemic serum of these dogs were used to simulate the minimum, median and maximum concentrations of triglycerides obtained during the lipemic state. Serum OS parameters were assessed using the antioxidants uric acid, albumin and total bilirubin; total antioxidant capacity (TAC); total oxidant capacity (TOC); and lipid peroxidation. In vivo PPL caused an increase in albumin, TAC-CUPRAC, TAC-FRAP, uric acid (p < 0.0001), TOC (p = 0.0012) and total bilirubin (p = 0.0245); reduction of TAC-ABTS (p = 0.0008); and did not alter the lipid peroxidation (p = 0.8983). In vitro, levels of albumin increased at the three lipemic concentrations (p < 0.0001), uric acid increased in the median and maximum levels (p < 0.0001), and total bilirubin concentration increased only at the maximum lipemic level (p = 0.0012). All lipemic levels tested increased TAC-ABTS (p = 0.0011) and TAC-FRAP (p < 0.0001). TAC-CUPRAC (p = 0.5002), TOC (p = 0.5938) and lipid peroxidation (p = 0.4235) were not affected by in vitro lipemia. In conclusion, both the in vivo postprandial state and in vitro simulated lipemia affect oxidative stress markers in dogs depending on the oxidative stress marker, and thus the postprandial state and/or lipemic samples should be avoided.
Research in Veterinary Science, May 1, 2021
Oxidative stress (OS) has been strongly associated with postprandial lipemia (PPL) in humans, and... more Oxidative stress (OS) has been strongly associated with postprandial lipemia (PPL) in humans, and still requires further investigation in dogs. However, since lipemia interferes with spectrophotometric determinations such as those used to assess OS, the present study investigated the effect of PPL on OS parameters of healthy dogs. Twenty dogs had lipemic postprandial samples compared to the average of two non-lipemic moments. Subsequently, PPL was simulated in vitro using a commercial lipid emulsion and twelve pools of non-lipemic serum of these dogs were used to simulate the minimum, median and maximum concentrations of triglycerides obtained during the lipemic state. Serum OS parameters were assessed using the antioxidants uric acid, albumin and total bilirubin; total antioxidant capacity (TAC); total oxidant capacity (TOC); and lipid peroxidation. In vivo PPL caused an increase in albumin, TAC-CUPRAC, TAC-FRAP, uric acid (p < 0.0001), TOC (p = 0.0012) and total bilirubin (p = 0.0245); reduction of TAC-ABTS (p = 0.0008); and did not alter the lipid peroxidation (p = 0.8983). In vitro, levels of albumin increased at the three lipemic concentrations (p < 0.0001), uric acid increased in the median and maximum levels (p < 0.0001), and total bilirubin concentration increased only at the maximum lipemic level (p = 0.0012). All lipemic levels tested increased TAC-ABTS (p = 0.0011) and TAC-FRAP (p < 0.0001). TAC-CUPRAC (p = 0.5002), TOC (p = 0.5938) and lipid peroxidation (p = 0.4235) were not affected by in vitro lipemia. In conclusion, both the in vivo postprandial state and in vitro simulated lipemia affect oxidative stress markers in dogs depending on the oxidative stress marker, and thus the postprandial state and/or lipemic samples should be avoided.
Foco, May 5, 2023
Obesity is a chronic disease that affects more than half of the canine population, predisposing t... more Obesity is a chronic disease that affects more than half of the canine population, predisposing to metabolic changes such as oxidative stress, which has already been associated with comorbidities. The aim of this study was to perform a systematic review and meta-analysis to identify whether systemic oxidative stress occurs in obese dogs. A systematic review of observational and experimental studies on oxidative stress in obese dogs was performed with no restriction on publication date and language. A total of 154 publications were located in four databases Pubmed (n=52), Web of Science (n=53), Gale (n=37) e Scopus (n=9)). After excluding duplicates and applying the selection criteria, all selected articles were blindly classified by reviewers according to their level of evidence. Using the Review Manager program (version 5.4), the degree of bias was calculated, and a meta-analysis of the randomized studies was performed. Most of the analyzed studies were classified as having a moderate level of evidence (2B and 3B), low methodological quality (JADAD = 0), high degree of bias and considerable heterogeneity (I² > 90%). The increase in lipid peroxidation (TBARS) and plasma leptin observed in observational studies was confirmed in randomized experimental studies and in the meta-analysis. Based on this systematic review, we conclude that there is a lack of studies on systemic oxidative stress in obese dogs and that it is necessary to expand research with a higher level of evidence and less bias. In our meta-analysis, among all markers, only the increase in TBARS and plasma leptin confirm systemic oxidative stress in obese dogs.
Foco, May 5, 2023
Obesity is a chronic disease that affects more than half of the canine population, predisposing t... more Obesity is a chronic disease that affects more than half of the canine population, predisposing to metabolic changes such as oxidative stress, which has already been associated with comorbidities. The aim of this study was to perform a systematic review and meta-analysis to identify whether systemic oxidative stress occurs in obese dogs. A systematic review of observational and experimental studies on oxidative stress in obese dogs was performed with no restriction on publication date and language. A total of 154 publications were located in four databases Pubmed (n=52), Web of Science (n=53), Gale (n=37) e Scopus (n=9)). After excluding duplicates and applying the selection criteria, all selected articles were blindly classified by reviewers according to their level of evidence. Using the Review Manager program (version 5.4), the degree of bias was calculated, and a meta-analysis of the randomized studies was performed. Most of the analyzed studies were classified as having a moderate level of evidence (2B and 3B), low methodological quality (JADAD = 0), high degree of bias and considerable heterogeneity (I² > 90%). The increase in lipid peroxidation (TBARS) and plasma leptin observed in observational studies was confirmed in randomized experimental studies and in the meta-analysis. Based on this systematic review, we conclude that there is a lack of studies on systemic oxidative stress in obese dogs and that it is necessary to expand research with a higher level of evidence and less bias. In our meta-analysis, among all markers, only the increase in TBARS and plasma leptin confirm systemic oxidative stress in obese dogs.
Eleven healthy capybara (Hydrochoerus hydrochaeris) from the Experimental Farm Lageado-FMVZ-UNESP... more Eleven healthy capybara (Hydrochoerus hydrochaeris) from the Experimental Farm Lageado-FMVZ-UNESP-Botucatu, SP-Brazil, were used in order to determine the hematological values, under Midazolan and Ketamine anesthesia. This study included the determination of normal eritrogram, leucogram, total plasmatic protein and fibrinogen values. The mean values for males and females were red cell 3.62 and 3.71x10 6 /µl, hemoglobin 15.0 and 15.4g/dl, PCV 48 and 49%, white cell 4630 and 5200/µl, total plasmatic protein 6.3 and 6.4mg/dl and fibrinogen 240 and 316.6mg/dl, respectively. Diference among sex was not found.
Eleven healthy capybara (Hydrochoerus hydrochaeris) from the Experimental Farm Lageado-FMVZ-UNESP... more Eleven healthy capybara (Hydrochoerus hydrochaeris) from the Experimental Farm Lageado-FMVZ-UNESP-Botucatu, SP-Brazil, were used in order to determine the hematological values, under Midazolan and Ketamine anesthesia. This study included the determination of normal eritrogram, leucogram, total plasmatic protein and fibrinogen values. The mean values for males and females were red cell 3.62 and 3.71x10 6 /µl, hemoglobin 15.0 and 15.4g/dl, PCV 48 and 49%, white cell 4630 and 5200/µl, total plasmatic protein 6.3 and 6.4mg/dl and fibrinogen 240 and 316.6mg/dl, respectively. Diference among sex was not found.
Brazilian Journal of Veterinary Research and Animal Science, Oct 18, 2003
Trabalhos 189 foram utilizadas amostras fecais de 96 animais divididos em 3 grupos: um grupo de 4... more Trabalhos 189 foram utilizadas amostras fecais de 96 animais divididos em 3 grupos: um grupo de 45 gatos jovens com até 10 meses de idade; outro, de 39 gatos adultos de 11 meses até 8 anos; por último, 12 gatos senis acima de 9 anos de idade. Todas as amostras foram submetidas a quatro técnicas coproparasitológicos: Wills, Faust, Hoffman e Safranina-azul de metileno. Foi encontrado um total de 52 animais positivos, sendo 26 gatos jovens, 20, adultos e 6 senis. No caso dos filhotes, os parasitas mais encontrados, em ordem decrescente, foram:
Brazilian Journal of Veterinary Research and Animal Science, Oct 18, 2003
Trabalhos 189 foram utilizadas amostras fecais de 96 animais divididos em 3 grupos: um grupo de 4... more Trabalhos 189 foram utilizadas amostras fecais de 96 animais divididos em 3 grupos: um grupo de 45 gatos jovens com até 10 meses de idade; outro, de 39 gatos adultos de 11 meses até 8 anos; por último, 12 gatos senis acima de 9 anos de idade. Todas as amostras foram submetidas a quatro técnicas coproparasitológicos: Wills, Faust, Hoffman e Safranina-azul de metileno. Foi encontrado um total de 52 animais positivos, sendo 26 gatos jovens, 20, adultos e 6 senis. No caso dos filhotes, os parasitas mais encontrados, em ordem decrescente, foram:
Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP, Jul 1, 2001
Transferência de imunidade passiva colostral e a morbidade e mortalidade bezerros neonatos Morbid... more Transferência de imunidade passiva colostral e a morbidade e mortalidade bezerros neonatos Morbidity and mortality of neonate calves related to immunity transfer by colostrum
Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP, Jul 1, 2001
Transferência de imunidade passiva colostral e a morbidade e mortalidade bezerros neonatos Morbid... more Transferência de imunidade passiva colostral e a morbidade e mortalidade bezerros neonatos Morbidity and mortality of neonate calves related to immunity transfer by colostrum
Ars Veterinaria, 2008
It was evaluated the influence of sex and age in proteinogram from newborn and young ostriches ra... more It was evaluated the influence of sex and age in proteinogram from newborn and young ostriches raised round Aracatuba, Sao Paulo. The total protein and its fractions in plasma samples were determined from 45 ostriches divided into three groups: 1-|15 days, 15-|30 days and 30-|70 days old. The total protein was measured using refractometric method and fractions were determined by electrophoresis on cellulose acetate membranes. There were not significant differences between males and females in values obtained for total protein in plasma (PPT), albumin, a , b , g -globulins. In ostriches until 15 days old, the PPT and fractions were higher (p<0,05) than those of ostriches with more 30 days old. The PPT and g -globulin of youger ostriches were higher (p<0,05) than those intermediate age. The results showed the metabolism of proteins in ostriches suffer significant changes in the first 70 days of age. KEY-WORDS : Ratite. Electrophoresis. Total protein in plasma. Age. Sex.
Ciencia Rural, Jul 24, 2009
Metabolismo oxidativo de heterófilos de avestruzes jovens.
Ciencia Rural, Jul 24, 2009
Metabolismo oxidativo de heterófilos de avestruzes jovens.
Revista Brasileira De Parasitologia Veterinaria, 2000
Revista Brasileira De Parasitologia Veterinaria, 2000
Research in Veterinary Science, May 1, 2021
Oxidative stress (OS) has been strongly associated with postprandial lipemia (PPL) in humans, and... more Oxidative stress (OS) has been strongly associated with postprandial lipemia (PPL) in humans, and still requires further investigation in dogs. However, since lipemia interferes with spectrophotometric determinations such as those used to assess OS, the present study investigated the effect of PPL on OS parameters of healthy dogs. Twenty dogs had lipemic postprandial samples compared to the average of two non-lipemic moments. Subsequently, PPL was simulated in vitro using a commercial lipid emulsion and twelve pools of non-lipemic serum of these dogs were used to simulate the minimum, median and maximum concentrations of triglycerides obtained during the lipemic state. Serum OS parameters were assessed using the antioxidants uric acid, albumin and total bilirubin; total antioxidant capacity (TAC); total oxidant capacity (TOC); and lipid peroxidation. In vivo PPL caused an increase in albumin, TAC-CUPRAC, TAC-FRAP, uric acid (p < 0.0001), TOC (p = 0.0012) and total bilirubin (p = 0.0245); reduction of TAC-ABTS (p = 0.0008); and did not alter the lipid peroxidation (p = 0.8983). In vitro, levels of albumin increased at the three lipemic concentrations (p < 0.0001), uric acid increased in the median and maximum levels (p < 0.0001), and total bilirubin concentration increased only at the maximum lipemic level (p = 0.0012). All lipemic levels tested increased TAC-ABTS (p = 0.0011) and TAC-FRAP (p < 0.0001). TAC-CUPRAC (p = 0.5002), TOC (p = 0.5938) and lipid peroxidation (p = 0.4235) were not affected by in vitro lipemia. In conclusion, both the in vivo postprandial state and in vitro simulated lipemia affect oxidative stress markers in dogs depending on the oxidative stress marker, and thus the postprandial state and/or lipemic samples should be avoided.
Research in Veterinary Science, May 1, 2021
Oxidative stress (OS) has been strongly associated with postprandial lipemia (PPL) in humans, and... more Oxidative stress (OS) has been strongly associated with postprandial lipemia (PPL) in humans, and still requires further investigation in dogs. However, since lipemia interferes with spectrophotometric determinations such as those used to assess OS, the present study investigated the effect of PPL on OS parameters of healthy dogs. Twenty dogs had lipemic postprandial samples compared to the average of two non-lipemic moments. Subsequently, PPL was simulated in vitro using a commercial lipid emulsion and twelve pools of non-lipemic serum of these dogs were used to simulate the minimum, median and maximum concentrations of triglycerides obtained during the lipemic state. Serum OS parameters were assessed using the antioxidants uric acid, albumin and total bilirubin; total antioxidant capacity (TAC); total oxidant capacity (TOC); and lipid peroxidation. In vivo PPL caused an increase in albumin, TAC-CUPRAC, TAC-FRAP, uric acid (p < 0.0001), TOC (p = 0.0012) and total bilirubin (p = 0.0245); reduction of TAC-ABTS (p = 0.0008); and did not alter the lipid peroxidation (p = 0.8983). In vitro, levels of albumin increased at the three lipemic concentrations (p < 0.0001), uric acid increased in the median and maximum levels (p < 0.0001), and total bilirubin concentration increased only at the maximum lipemic level (p = 0.0012). All lipemic levels tested increased TAC-ABTS (p = 0.0011) and TAC-FRAP (p < 0.0001). TAC-CUPRAC (p = 0.5002), TOC (p = 0.5938) and lipid peroxidation (p = 0.4235) were not affected by in vitro lipemia. In conclusion, both the in vivo postprandial state and in vitro simulated lipemia affect oxidative stress markers in dogs depending on the oxidative stress marker, and thus the postprandial state and/or lipemic samples should be avoided.
Foco, May 5, 2023
Obesity is a chronic disease that affects more than half of the canine population, predisposing t... more Obesity is a chronic disease that affects more than half of the canine population, predisposing to metabolic changes such as oxidative stress, which has already been associated with comorbidities. The aim of this study was to perform a systematic review and meta-analysis to identify whether systemic oxidative stress occurs in obese dogs. A systematic review of observational and experimental studies on oxidative stress in obese dogs was performed with no restriction on publication date and language. A total of 154 publications were located in four databases Pubmed (n=52), Web of Science (n=53), Gale (n=37) e Scopus (n=9)). After excluding duplicates and applying the selection criteria, all selected articles were blindly classified by reviewers according to their level of evidence. Using the Review Manager program (version 5.4), the degree of bias was calculated, and a meta-analysis of the randomized studies was performed. Most of the analyzed studies were classified as having a moderate level of evidence (2B and 3B), low methodological quality (JADAD = 0), high degree of bias and considerable heterogeneity (I² > 90%). The increase in lipid peroxidation (TBARS) and plasma leptin observed in observational studies was confirmed in randomized experimental studies and in the meta-analysis. Based on this systematic review, we conclude that there is a lack of studies on systemic oxidative stress in obese dogs and that it is necessary to expand research with a higher level of evidence and less bias. In our meta-analysis, among all markers, only the increase in TBARS and plasma leptin confirm systemic oxidative stress in obese dogs.
Foco, May 5, 2023
Obesity is a chronic disease that affects more than half of the canine population, predisposing t... more Obesity is a chronic disease that affects more than half of the canine population, predisposing to metabolic changes such as oxidative stress, which has already been associated with comorbidities. The aim of this study was to perform a systematic review and meta-analysis to identify whether systemic oxidative stress occurs in obese dogs. A systematic review of observational and experimental studies on oxidative stress in obese dogs was performed with no restriction on publication date and language. A total of 154 publications were located in four databases Pubmed (n=52), Web of Science (n=53), Gale (n=37) e Scopus (n=9)). After excluding duplicates and applying the selection criteria, all selected articles were blindly classified by reviewers according to their level of evidence. Using the Review Manager program (version 5.4), the degree of bias was calculated, and a meta-analysis of the randomized studies was performed. Most of the analyzed studies were classified as having a moderate level of evidence (2B and 3B), low methodological quality (JADAD = 0), high degree of bias and considerable heterogeneity (I² > 90%). The increase in lipid peroxidation (TBARS) and plasma leptin observed in observational studies was confirmed in randomized experimental studies and in the meta-analysis. Based on this systematic review, we conclude that there is a lack of studies on systemic oxidative stress in obese dogs and that it is necessary to expand research with a higher level of evidence and less bias. In our meta-analysis, among all markers, only the increase in TBARS and plasma leptin confirm systemic oxidative stress in obese dogs.
Eleven healthy capybara (Hydrochoerus hydrochaeris) from the Experimental Farm Lageado-FMVZ-UNESP... more Eleven healthy capybara (Hydrochoerus hydrochaeris) from the Experimental Farm Lageado-FMVZ-UNESP-Botucatu, SP-Brazil, were used in order to determine the hematological values, under Midazolan and Ketamine anesthesia. This study included the determination of normal eritrogram, leucogram, total plasmatic protein and fibrinogen values. The mean values for males and females were red cell 3.62 and 3.71x10 6 /µl, hemoglobin 15.0 and 15.4g/dl, PCV 48 and 49%, white cell 4630 and 5200/µl, total plasmatic protein 6.3 and 6.4mg/dl and fibrinogen 240 and 316.6mg/dl, respectively. Diference among sex was not found.
Eleven healthy capybara (Hydrochoerus hydrochaeris) from the Experimental Farm Lageado-FMVZ-UNESP... more Eleven healthy capybara (Hydrochoerus hydrochaeris) from the Experimental Farm Lageado-FMVZ-UNESP-Botucatu, SP-Brazil, were used in order to determine the hematological values, under Midazolan and Ketamine anesthesia. This study included the determination of normal eritrogram, leucogram, total plasmatic protein and fibrinogen values. The mean values for males and females were red cell 3.62 and 3.71x10 6 /µl, hemoglobin 15.0 and 15.4g/dl, PCV 48 and 49%, white cell 4630 and 5200/µl, total plasmatic protein 6.3 and 6.4mg/dl and fibrinogen 240 and 316.6mg/dl, respectively. Diference among sex was not found.
Brazilian Journal of Veterinary Research and Animal Science, Oct 18, 2003
Trabalhos 189 foram utilizadas amostras fecais de 96 animais divididos em 3 grupos: um grupo de 4... more Trabalhos 189 foram utilizadas amostras fecais de 96 animais divididos em 3 grupos: um grupo de 45 gatos jovens com até 10 meses de idade; outro, de 39 gatos adultos de 11 meses até 8 anos; por último, 12 gatos senis acima de 9 anos de idade. Todas as amostras foram submetidas a quatro técnicas coproparasitológicos: Wills, Faust, Hoffman e Safranina-azul de metileno. Foi encontrado um total de 52 animais positivos, sendo 26 gatos jovens, 20, adultos e 6 senis. No caso dos filhotes, os parasitas mais encontrados, em ordem decrescente, foram:
Brazilian Journal of Veterinary Research and Animal Science, Oct 18, 2003
Trabalhos 189 foram utilizadas amostras fecais de 96 animais divididos em 3 grupos: um grupo de 4... more Trabalhos 189 foram utilizadas amostras fecais de 96 animais divididos em 3 grupos: um grupo de 45 gatos jovens com até 10 meses de idade; outro, de 39 gatos adultos de 11 meses até 8 anos; por último, 12 gatos senis acima de 9 anos de idade. Todas as amostras foram submetidas a quatro técnicas coproparasitológicos: Wills, Faust, Hoffman e Safranina-azul de metileno. Foi encontrado um total de 52 animais positivos, sendo 26 gatos jovens, 20, adultos e 6 senis. No caso dos filhotes, os parasitas mais encontrados, em ordem decrescente, foram:
Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP, Jul 1, 2001
Transferência de imunidade passiva colostral e a morbidade e mortalidade bezerros neonatos Morbid... more Transferência de imunidade passiva colostral e a morbidade e mortalidade bezerros neonatos Morbidity and mortality of neonate calves related to immunity transfer by colostrum
Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP, Jul 1, 2001
Transferência de imunidade passiva colostral e a morbidade e mortalidade bezerros neonatos Morbid... more Transferência de imunidade passiva colostral e a morbidade e mortalidade bezerros neonatos Morbidity and mortality of neonate calves related to immunity transfer by colostrum