Pedro Sebastián Pereyra Robles - Academia.edu (original) (raw)
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Papers by Pedro Sebastián Pereyra Robles
The International Journal of Developmental Biology, 2005
The study of flower development has experienced great advances over the last 15 years. The most i... more The study of flower development has experienced great advances over the last 15 years. The most important landmark was the proposal of the ABC model in which three different functions of overlapping activities account for the development of the four rings of organs of the eudicot flower. Most interestingly, during recent years this simple and elegant model has been broadly accepted and is applicable to a wide range of plant species. However, recent advances in the characterization of protein interactions and the discovery of the SEPALLATA genes that are required for proper floral organ development have led to a revision of the ABC model. The largely accepted floral quartet model, which includes the new SEPALLATA function, postulates that the development of a specific floral organ is achieved by the formation of a single complex of different MADS-box proteins. The ultimate fate of the flower is to become a fruit, ensuring dispersal of the seeds and therefore survival of the species. The Arabidopsis fruit is a silique or pod. Only in the last five years important advances have been made in establishing the differentiation of the tissues required for the opening of the fruit: the valve margins and dehiscence zone. Classical genetic analyses and molecular biology approaches have pointed to the involvement of the transcription factors SHP, ALC and IND in the formation of these tissues and of FUL and RPL in repressing this identity in the bordering tissues, valves and replum, respectively.
Current Biology, 2004
The SEP4 Gene of Arabidopsis thaliana Functions in Floral Organ and Meristem Identity triple muta... more The SEP4 Gene of Arabidopsis thaliana Functions in Floral Organ and Meristem Identity triple mutant, all flower organs are converted into sepals despite the fact that the onset of B and C gene expression is not affected in the triple mutant [3, 4]. The conver
The International Journal of Developmental Biology, 2009
Large-scale exploratory approaches to understanding gene function laid the foundations for the "-... more Large-scale exploratory approaches to understanding gene function laid the foundations for the "-omics era". Based on modern technologies for the structural and functional characterization of genomes, these curiosity-driven approaches allow systematic accumulation of vast amounts of data, enabling subsequent hypothesis-driven research. Some years before the dawn of genomics, exploratory approaches were already furthering our understanding of gene function in the form of saturation mutagenesis experiments aimed at the identification of all genes that mutate to a given phenotype. Forward genetic approaches, conducted on experimental organisms such as Drosophila melanogaster and Caenorhabditis elegans, have led to the isolation of mutants affected in specific developmental processes, whose cellular and molecular characterization has unraveled the underlying genetic mechanisms of animal development. To shed light on the making of plant leaves, in 1993 we initiated an attempt to identify as many viable and fertile mutants with abnormal leaf morphology as possible, using the Arabidopsis thaliana model organism. We identified 25 fast-neutron-and 153 ethyl-methane sulfonate-induced mutations, which fell into eight and 94 complementation groups, respectively. We also studied 115 publicly available mutant lines isolated by previous authors, which fell into 37 complementation groups. Although we did not reach saturation of the Arabidopsis thaliana genome, the broad spectrum of leaf morphological alterations identified is facilitating the dissection of specific leaf developmental processes. In a complementary approach, we also analyzed leaf architecture in natural accessions and two populations of recombinant inbred lines. Using a high-throughput gene mapping method, we have already cloned 25 of the genes identified by mutation, in some cases in collaboration with other groups. The products of these genes participate in various developmental processes, such as polar cell expansion, transduction of hormonal signals, gene regulation, plastid biogenesis, and chromatin remodeling, among others. The range of phenotypes and processes identified reveal the complexity of leaf ontogeny and will help explain the diversity of leaf morphology in nature.
Genetics, 2000
In an attempt to identify genes involved in the control of leaf morphogenesis, we have studied 13... more In an attempt to identify genes involved in the control of leaf morphogenesis, we have studied 13 Arabidopsis thaliana mutants with curled, involute leaves, a phenotype herein referred to as Incurvata (Icu), which were isolated by G. Röbbelen and belong to the Arabidopsis ...
The International Journal of Developmental Biology, 2005
The study of flower development has experienced great advances over the last 15 years. The most i... more The study of flower development has experienced great advances over the last 15 years. The most important landmark was the proposal of the ABC model in which three different functions of overlapping activities account for the development of the four rings of organs of the eudicot flower. Most interestingly, during recent years this simple and elegant model has been broadly accepted and is applicable to a wide range of plant species. However, recent advances in the characterization of protein interactions and the discovery of the SEPALLATA genes that are required for proper floral organ development have led to a revision of the ABC model. The largely accepted floral quartet model, which includes the new SEPALLATA function, postulates that the development of a specific floral organ is achieved by the formation of a single complex of different MADS-box proteins. The ultimate fate of the flower is to become a fruit, ensuring dispersal of the seeds and therefore survival of the species. The Arabidopsis fruit is a silique or pod. Only in the last five years important advances have been made in establishing the differentiation of the tissues required for the opening of the fruit: the valve margins and dehiscence zone. Classical genetic analyses and molecular biology approaches have pointed to the involvement of the transcription factors SHP, ALC and IND in the formation of these tissues and of FUL and RPL in repressing this identity in the bordering tissues, valves and replum, respectively.
Current Biology, 2004
The SEP4 Gene of Arabidopsis thaliana Functions in Floral Organ and Meristem Identity triple muta... more The SEP4 Gene of Arabidopsis thaliana Functions in Floral Organ and Meristem Identity triple mutant, all flower organs are converted into sepals despite the fact that the onset of B and C gene expression is not affected in the triple mutant [3, 4]. The conver
The International Journal of Developmental Biology, 2009
Large-scale exploratory approaches to understanding gene function laid the foundations for the "-... more Large-scale exploratory approaches to understanding gene function laid the foundations for the "-omics era". Based on modern technologies for the structural and functional characterization of genomes, these curiosity-driven approaches allow systematic accumulation of vast amounts of data, enabling subsequent hypothesis-driven research. Some years before the dawn of genomics, exploratory approaches were already furthering our understanding of gene function in the form of saturation mutagenesis experiments aimed at the identification of all genes that mutate to a given phenotype. Forward genetic approaches, conducted on experimental organisms such as Drosophila melanogaster and Caenorhabditis elegans, have led to the isolation of mutants affected in specific developmental processes, whose cellular and molecular characterization has unraveled the underlying genetic mechanisms of animal development. To shed light on the making of plant leaves, in 1993 we initiated an attempt to identify as many viable and fertile mutants with abnormal leaf morphology as possible, using the Arabidopsis thaliana model organism. We identified 25 fast-neutron-and 153 ethyl-methane sulfonate-induced mutations, which fell into eight and 94 complementation groups, respectively. We also studied 115 publicly available mutant lines isolated by previous authors, which fell into 37 complementation groups. Although we did not reach saturation of the Arabidopsis thaliana genome, the broad spectrum of leaf morphological alterations identified is facilitating the dissection of specific leaf developmental processes. In a complementary approach, we also analyzed leaf architecture in natural accessions and two populations of recombinant inbred lines. Using a high-throughput gene mapping method, we have already cloned 25 of the genes identified by mutation, in some cases in collaboration with other groups. The products of these genes participate in various developmental processes, such as polar cell expansion, transduction of hormonal signals, gene regulation, plastid biogenesis, and chromatin remodeling, among others. The range of phenotypes and processes identified reveal the complexity of leaf ontogeny and will help explain the diversity of leaf morphology in nature.
Genetics, 2000
In an attempt to identify genes involved in the control of leaf morphogenesis, we have studied 13... more In an attempt to identify genes involved in the control of leaf morphogenesis, we have studied 13 Arabidopsis thaliana mutants with curled, involute leaves, a phenotype herein referred to as Incurvata (Icu), which were isolated by G. Röbbelen and belong to the Arabidopsis ...