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Papers by Peter Kasa
Cell and Tissue Research, 1990
Experientia, 1969
ABSTRACT
Brain Research, Aug 3, 2001
The axonal transport of presenilin-1 was investigated in a spinal cord-sciatic nerve-neuromuscula... more The axonal transport of presenilin-1 was investigated in a spinal cord-sciatic nerve-neuromuscular junction model system in the rat. The technique of unilateral sciatic nerve ligation, using double ligatures, was combined with immunohistochemical staining and Western blotting to examine the axonal transport of the protein. Immunohistochemical studies involving the use of polyclonal antibodies for either the N-terminal or the C-terminal domain of presenilin-1 furnished evidence that both fragments may be present not only in the neuronal cell bodies, but also in the motoric and sensory axons and the motoric axon terminals at the neuromuscular junctions. After double ligation of the sciatic nerve for 6, 12 or 24 h, progressive immunostaining of presenilin-1 occurred above the upper ligature and to a lesser extent below the lower ligature. Double staining of the sciatic nerve for presenilin-1 and for amyloid precursor protein revealed overlapping immunoreactivity. Western blotting confirmed the accumulation of the |20-kDa C-terminal and |25-kDa N-terminal fragments and the full-length 45-kDa holoprotein of presenilin-1 both above and below the ligature. It is concluded that besides the larger amounts of C-terminal and N-terminal fragments, a smaller quantity of intact presenilin-1 may be present and conveyed bidirectionally in the sciatic nerve of the rat. These results lend further support to the suggestion that presenilin-1 may leave the trans-Golgi network and be found in the axons and axon terminals of the various neurons.
CITATIONS 2 READS 18 5 authors, including:
Histochemistry Cell Biol, 1968
Acta Biologica Hungarica, 2002
Journal of Neurochemistry
Journal of Neurochemistry
It is assumed that the amyloid-beta peptide (A ABSTRACT β) contributes to the neurodegen- eration... more It is assumed that the amyloid-beta peptide (A ABSTRACT β) contributes to the neurodegen- eration in Alzheimer's disease (AD). Activation of an apoptotic pathway may play a key role in this process. The apoptotic signal may be driven by caspases. The presynaptic Aβ protein may be an activator of caspase-3 and could initiate a series of cascade events, which results in neurofi brillary degeneration in a postsynaptic cell. We report here that the axonic Aβ in the AD brain may be associated with caspase-3 activation. Our data suggest that caspase-3 in fact has a signifi cant role in the widespread neuronal cell death that occurs in AD brain. A subset of pyramidal cells in hippocampus area CA1 demonstrated widespread accumulation of tau- protein. Individual postsynaptic neurons contained intracellular activated caspase-3 and were co-localized with neurofi brillary tangles. The results presented here support the suggestion that caspase-3 activation may lead to the neuronal cell death...
Neurochemistry International
Neurochemistry International
Advances in Behavioral Biology, 2002
... Henrietta Papp and Magdolna Pakaski, Alzheimer's Disease Research Centre, Department of ... more ... Henrietta Papp and Magdolna Pakaski, Alzheimer's Disease Research Centre, Department of Psychiatry. ... This work was supported by OTKA (T022683, T030339 and T032458), ETT (T-11/011/2000) and a Szechenyi Professorship to PK REFERENCES 1. DM Kovacs, H J ...
Cell and Tissue Research, 1990
Experientia, 1969
ABSTRACT
Brain Research, Aug 3, 2001
The axonal transport of presenilin-1 was investigated in a spinal cord-sciatic nerve-neuromuscula... more The axonal transport of presenilin-1 was investigated in a spinal cord-sciatic nerve-neuromuscular junction model system in the rat. The technique of unilateral sciatic nerve ligation, using double ligatures, was combined with immunohistochemical staining and Western blotting to examine the axonal transport of the protein. Immunohistochemical studies involving the use of polyclonal antibodies for either the N-terminal or the C-terminal domain of presenilin-1 furnished evidence that both fragments may be present not only in the neuronal cell bodies, but also in the motoric and sensory axons and the motoric axon terminals at the neuromuscular junctions. After double ligation of the sciatic nerve for 6, 12 or 24 h, progressive immunostaining of presenilin-1 occurred above the upper ligature and to a lesser extent below the lower ligature. Double staining of the sciatic nerve for presenilin-1 and for amyloid precursor protein revealed overlapping immunoreactivity. Western blotting confirmed the accumulation of the |20-kDa C-terminal and |25-kDa N-terminal fragments and the full-length 45-kDa holoprotein of presenilin-1 both above and below the ligature. It is concluded that besides the larger amounts of C-terminal and N-terminal fragments, a smaller quantity of intact presenilin-1 may be present and conveyed bidirectionally in the sciatic nerve of the rat. These results lend further support to the suggestion that presenilin-1 may leave the trans-Golgi network and be found in the axons and axon terminals of the various neurons.
CITATIONS 2 READS 18 5 authors, including:
Histochemistry Cell Biol, 1968
Acta Biologica Hungarica, 2002
Journal of Neurochemistry
Journal of Neurochemistry
It is assumed that the amyloid-beta peptide (A ABSTRACT β) contributes to the neurodegen- eration... more It is assumed that the amyloid-beta peptide (A ABSTRACT β) contributes to the neurodegen- eration in Alzheimer's disease (AD). Activation of an apoptotic pathway may play a key role in this process. The apoptotic signal may be driven by caspases. The presynaptic Aβ protein may be an activator of caspase-3 and could initiate a series of cascade events, which results in neurofi brillary degeneration in a postsynaptic cell. We report here that the axonic Aβ in the AD brain may be associated with caspase-3 activation. Our data suggest that caspase-3 in fact has a signifi cant role in the widespread neuronal cell death that occurs in AD brain. A subset of pyramidal cells in hippocampus area CA1 demonstrated widespread accumulation of tau- protein. Individual postsynaptic neurons contained intracellular activated caspase-3 and were co-localized with neurofi brillary tangles. The results presented here support the suggestion that caspase-3 activation may lead to the neuronal cell death...
Neurochemistry International
Neurochemistry International
Advances in Behavioral Biology, 2002
... Henrietta Papp and Magdolna Pakaski, Alzheimer's Disease Research Centre, Department of ... more ... Henrietta Papp and Magdolna Pakaski, Alzheimer's Disease Research Centre, Department of Psychiatry. ... This work was supported by OTKA (T022683, T030339 and T032458), ETT (T-11/011/2000) and a Szechenyi Professorship to PK REFERENCES 1. DM Kovacs, H J ...