Peter Massanyi - Academia.edu (original) (raw)
Papers by Peter Massanyi
Veterinarni Medicina, Oct 15, 2022
Currently, in animal nutrition, the replacement of synthetic substances with natural ones was exp... more Currently, in animal nutrition, the replacement of synthetic substances with natural ones was expected to improve animal health. The aim of the present study was to evaluate the effects of a dietary brown seaweed and plant polyphenol mixture in adult male rabbits on the haematological profile and antioxidant markers. Twentyfour adult male rabbits were divided into three experimental groups receiving a control diet (C) or diets supplemented with 0.3% (T1) and 0.6% (T2) of a feed additive containing brown seaweed (Laminaria spp.) and plant extracts of seaweed origin. The trial lasted for 90 days. A lower potassium concentration was observed at 30 days in the T2 group, compared with the T1 and C groups. An increase in the antioxidant status was observed (P < 0.05) from day 60 of the trial in the rabbits fed diets with an algae-polyphenolic supplement (T1 and T2 groups). Concluding, the diet supplementation of brown seaweed and polyphenol stimulates the antioxidant status of the blood, however, it does not affect the haematological profile.
Biological Trace Element Research, Jul 3, 2018
The monitoring of various elements in the cow milk is important in the agricultural sector. The g... more The monitoring of various elements in the cow milk is important in the agricultural sector. The goal of this study was to determine the concentrations of calcium (Ca), cadmium (Cd), copper (Cu), iron (Fe), mercury (Hg), potassium (K), magnesium (Mg), sodium (Na), nickel (Ni), lead (Pb), and zinc (Zn) in the milk samples coming from different origins (local region in Nitra, nationwide sample represents the common Slovak brands of milk and common brands of milk from Czech Republic widely available in Slovakia). The samples were analyzed using atomic absorption spectrometry. A high level of variability in the Cu, Fe, Na, Ni, and Zn contents was observed. Magnesium was comparatively high in the milk sample from Slovakia (273.23 ± 16.32 μg/mL) against sample from Nitra (230.91 ± 9.48 μg/mL) and Czech Republic (202.70 ± 10.83 μg/mL). Potassium was significantly higher in milk from Nitra region (3301.98 ± 95.66) against SK sample (2925.16 ± 75.74 μg/mL). There were no significant differences in other elements among the three regions. The provisional tolerable weekly intake (PTWI) contribution suggested low dietary exposure to observed toxic metals (Cd, Hg, and Pb) in milk samples. Margin of exposure (MOE) evaluation denoted that even higher consumption of milk poses no high cardiovascular and nephrotoxicity threat. However, Cd and Pb are known for their cumulative effect and the monitoring of these elements in milk is strongly required.
Folia Biologica
The aim of this in vitro study was to examine the dose-dependent effects of iron as a potential e... more The aim of this in vitro study was to examine the dose-dependent effects of iron as a potential endocrine disruptor in relation to the release of sexual steroid hormones by a human adrenocortical carcinoma (NCI-H295R) cell line. The cells were exposed to different concentrations (3.90, 62.50, 250, 500, 1000 μM) of FeSO4.7H2O and compared with the control group (culture medium without FeSO4.7H2O). Cell viability was measured by the metabolic activity assay. Quantification of sexual steroid production was performed by enzyme-linked immunosorbent assay. Following 48 h culture of the cells in the presence of FeSO4.7H2O, significantly (P < 0.001) increased production of progesterone was observed at the lowest concentration (3.90 μM) of FeSO4.7H2O, whereas the lowest release of progesterone by NCIH295R cells was noted after addition of 1000 μM of FeSO4.7H2O, which did not elicit cytotoxic action (P > 0.05). Testosterone production was substantially increased at the concentrations ≤ ...
The purpose of this study was to monitor concentration of selected biological and risk elements i... more The purpose of this study was to monitor concentration of selected biological and risk elements in testes and later epididymal spermatozoa motility of wild boars (Sus scrofa scrofa) as well as their association. Wild boars were hunted in region Žuhračka - Levice branch plant, Slovak Republic. Testes were surgically removed post-mortem and were transported to the laboratory. Spermatozoa were obtained by dissecting the distal portion of the cauda epididymis and were analysed by Computer assisted semen analyzer (CASA) system. Concentration of elements were measured by inductively-coupled plasma optical emission spectrometry (ICP-OES) and by cold vapor absorption spectrometer (MA-3 Solo Mercury Analyzer). Total spermatozoa motility was at the level of 44.29% and progressive motility was 18.47%. Concentration of elements in testes was in following order: K > Na > Mg > Ca > Fe > Zn > Al > Cu > Se > Mn > As > Cr > Pb > Mo > Sr > Ni > Ba > ...
Toxics
Various studies have shown that the reproductive organs are highly sensitive to toxic elements fo... more Various studies have shown that the reproductive organs are highly sensitive to toxic elements found in the environment. Due to technological progress, the use of nanoparticles has become more common nowadays. Nanoparticles are used for drug delivery because their dimensions allow them to circulate throughout the body and enter directly into the cell. Antimicrobial properties are increasingly used in the manufacture of medical devices, textiles, food packaging, cosmetics, and other consumer products. Nanoparticles provide several benefits, but aspects related to their effects on living organisms and the environment are not well known. This review summarizes current in vivo, and in vitro animal studies focused on the evaluation of toxicity of selected metal nanoparticles (Ag, ZnO, TiO2) on male and female reproductive health. It can be concluded that higher concentrations of metal nanoparticles in the male reproductive system can cause a decrease in spermatozoa motility, viability an...
Journal of microbiology, biotechnology and food sciences
The objective of the present study was to investigate semen quality of infertile smokers and non-... more The objective of the present study was to investigate semen quality of infertile smokers and non-smokers including Benzo[a]Pyrene-7,8-diol-9,10 epoxide (BPDE)-DNA adduct formation and acrosome status of sperm cells. Semen quality parameters were analyzed according to 2010 World Health Organization criteria in 122 non-smokers and 125 cigarette smokers attending tertiary health care centre in Barak Valley of Assam, India followed by BPDE-DNA adduct formation and acrosome status test. A significant decline was found in sperm concentration, progressive motility, morphology in smokers (p
The Journal of Microbiology, Biotechnology and Food Sciences, Jan 6, 2021
The aim of this study was to determine the effect of dried strawberry leaves inclusion on selecte... more The aim of this study was to determine the effect of dried strawberry leaves inclusion on selected haematological parameters of rabbits: white blood cell, red blood cell, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, platelets, mean platelet volume, red cell distribution width, lymphocytes, mid cells total count, granulocytes, haematocrit, platelet distribution width. Thirtyrabbits of broiler line Californian were used in this experiment. The animals were divided into the four groups, four animals in each one (control group C and experimental groups E1, E2 and E3). Animals were fed ad libitum using KKV1 feeding mixture (FM) with or without strawberry leave inclusion as follows: group E1 received feed mixture with concentration of 0.5 % strawberry leaves group E2 1.0 % and group E3 1.5 %. Bloodwas collectedinto tubes and the parameters were analysed using Abacus Junior Vet. Statistical analyse showed a significant change (P <0.05) in mean platelet volume between group C and E1 and between group C and E2. Increase of this parameter was detected in all groups in comparison with the control group. Other haematological parameters were without significant differences (P>0.05). Strawberry leaves as a source of antioxidants altered haematological parameters according to our expectations. This study presents only partial results of continuing experiment.
Journal of Environmental Science and Health, Part A, 2021
Abstract The purpose of the present study was to evaluate the effects of aflatoxin B1 (AFB1) and ... more Abstract The purpose of the present study was to evaluate the effects of aflatoxin B1 (AFB1) and benzo[a]pyrene (BaP) on the heart muscle of chicken embryos of the broiler line Ross 308. The benzo[a]pyrene in the organic oil solution was injected in ovo on the 6th day of the incubation in doses of: 0.1, 0.5, and 1 mg/kg weight of eggs; the aflatoxin B1 in the organic oil solution was injected in ovo on the 6th day of the incubation into the yolk in doses of 80, 120 and 240 ng/kg weight of eggs. Multiple biochemical and hepatic parameters have been observed, including sodium, potassium, chloride, cholesterol, uric acid, total proteins, aminotransferase aspartate, and aminotransferase alanine. A low dose of AFB1 and BaP administered in ovo during early embryonic development had a significant impact on chicken embryonic development, as demonstrated by alterations in biochemical, mineral, and hepatic parameters.
The Journal of Microbiology, Biotechnology and Food Sciences, 2017
Acrylamide monomer causes neurotoxicity, male reproductive toxicity and endocrine – related tumor... more Acrylamide monomer causes neurotoxicity, male reproductive toxicity and endocrine – related tumors in rodents. Acrylamide was found in food, heat-processed potato products, coffee, bread and has been classified as probable human carcinogen by IARC. Daily intake from foodstuff was estimated in the range of 0.3-0.8 mg/kg bw per day (Seal et al., 2008). Glutathione peroxidase catalyzes the reduction hydrogen peroxide by glutathione. Extracellular superoxide dismutase catalyzes the dismutation of superoxide radical to hydrogen peroxide and oxygen. The activity of superoxide dismutase and glutathione peroxidase has been examined. The research was conducted on SWISS mice 8 week old, weight 26 g. Decapitation was carried out after 48, 72 and 192 hours after acrylamide injection. Animals received two doses of acrylamide 20 mg/kg and 40 mg/kg with intraperitoneal injection three times a week. Control group received physiological saline 3 times a week. The activity of superoxide dismutase was...
Universal Journal of Agricultural Research, 2014
In this study distribution of nickel as a risk factor of environment in testis and its effects on... more In this study distribution of nickel as a risk factor of environment in testis and its effects on the testicular structure in experimental animals and effect on spermatozoa was analyzed. In this study the effect of Ni on the testicular structure after an experimental intraperitoneal (i.p.) administration, concentration of nickel in semen of different animal species and the effect of in vitro spermatozoa incubation with nickel on the spermatozoa motility and membrane changes is reported. Our findings clearly suggest a negative effect of nickel on the structure as well as on the function of seminiferous epithelium. In experimental groups with nickel a significant (p 250 μM Ni/ml) cause decrease of spermatozoa motility in vitro.
Slovak Journal of Animal Science, 2014
In the present study the effect of collection regime on rabbit semen development was analyzed. Ei... more In the present study the effect of collection regime on rabbit semen development was analyzed. Eight seminal traits, such as overall ejaculate volume (VOL), spermatozoa concentration (CON), motility (MOT), progressive motility (PRO), percentage of progressive motility (% PRO), curvilinear velocity (VCL), beat-cross frequency (BCF) and amplitude of lateral head displacement (ALH) were analyzed. As the number of collections increased, more bucks were able to ejaculate. During collections the semen volume gradually increased from 0.4 ml to 1.0 ml with the average of 0.68 ± 0.34 ml. The average of MOT was 55.55 ± 26.63 % and the average of PRO reached 40.43 ± 27.35 %. The other assessed parameters were CON - 0.547 ± 0.165 x 10 9 ml -1 ; ALH - 4.18 ± 1.18 µm; BCF - 27.42 ± 7.12 Hz; VCL - 104.04 ± 37.00 µm.s -1 �� A positive correlation between ejaculate volume and the number of collections was found. MOT and PRO significantly correlated with the number of collections. In addition, positi...
The aim of this study was to observe the effect of age on selected biochemical parameters of hors... more The aim of this study was to observe the effect of age on selected biochemical parameters of horses' blood. Horses were divided in four age categories (5, 6, 7 and 9 years old). Blood was collected from 31 horses (Furioso breed). Assessment of biochemical parameters was realized by Randox RX Monza analyzer. Significant differences were observed between different groups in total proteins. Concentrations of calcium, phosphorus, sodium, potassium and chlorides correlate with the age of the animals. Similarly, liver enzyme activities correspond to age groups. In the other monitored biochemical parameters any significant differences between the groups were found. The achieved results confirm the connection between age of horses and values of biochemical parameters.
Slovak Journal of Animal Science, 2009
concentrations of cooper, zinc, iron, manganese, cadmium, lead and nickel in the semen of rabbits... more concentrations of cooper, zinc, iron, manganese, cadmium, lead and nickel in the semen of rabbits (n=27) in relation to spermato zoa quality were evaluated. samples were analyzed on an atomic absorption spectrophotometer (Unicam solar 939).
Spermatozoa - Facts and Perspectives, 2018
Oxidative stress (OS) is a condition caused by an imbalance between reactive oxygen species (ROS)... more Oxidative stress (OS) is a condition caused by an imbalance between reactive oxygen species (ROS) overgeneration and decreased antioxidant defense mechanisms in the cell. OS has become a prominent factor in male reproductive dysfunction as ROS cause damage to sperm DNA, lipids and proteins, alterations to critical sperm structures and signaling pathways, leading to a decreased sperm activity and fertilizing capacity. At the same time, small amounts of ROS play vital roles in events leading to sperm maturation and acquisition of functional activity, which is why a proper oxidative balance is of paramount importance for a proper male fertility. Understanding the physiological and pathological roles of ROS in male reproduction has become an essential pillar of modern andrology; however, numerous questions related to the controversial behavior of ROS in male reproductive cells and tissues still remain unanswered. This chapter aims to summarize current evidence available on the relationships between free radicals, antioxidants and male reproduction and to trigger more scientific interest, particularly with respect to the design of efficient strategies to diagnose or treat male sub-or infertility associated with OS.
Physiological Research, 2019
The target of this study was to evaluate the effect of extract of the European mistletoe – Viscum... more The target of this study was to evaluate the effect of extract of the European mistletoe – Viscum album quercus L. on spermatozoa motility and viability in vitro. The CASA system was used to determine the spermatozoa motility parameters at different time intervals (0, 1, 2 and 3 h) and spermatozoa viability was determined in five different doses of Viscum album quercus L [10 (QA), 6.6 (QB), 3.3 (QC), 2.5 (QD) and 2 (QE) mg/ml]. Results in experimental groups detected a significant deterioration on rabbit spermatozoa after 1, 2 and 3 hours, compared to the control. The initial total spermatozoa motility showed increased value for all doses of Viscum album quercus in comparison to control. After in vitro culture a dose–dependent decrease (QA: reduction of 69.7 %, QB: reduction of 40.9 %) was found. For the progressive spermatozoa most significant decrease (86.8 % for QA vs. 48.5 % for QB) was detected compared to the control after 3 hours of culture. Spermatozoa viability (MTT test) w...
Physiological Research, 2020
Nickel is a ubiquitous environmental pollutant, which has various effects on reproductive endocri... more Nickel is a ubiquitous environmental pollutant, which has various effects on reproductive endocrinology. In this study, human adrenocortical carcinoma (NCI-H295R) cell line was used as an in vitro biological model to study the effect of nickel chloride (NiCl2) on the viability and steroidogenesis. The cells were exposed to different concentrations (3.90; 7.80; 15.60; 31.20; 62.50; 125; 250 and 500 μM) of NiCl2 and compared with control group (culture medium without NiCl2). The cell viability was measured by the metabolic activity assay. Production of sexual steroid hormones was quantified by enzyme linked immunosorbent assay. Following 48 h culture of the cells in the presence of NiCl2 a dose-dependent depletion of progesterone release was observed even at the lower concentrations. In fact, lower levels of progesterone were detected in groups with higher doses (≥125 μM) of NiCl2 (P<0.01), which also elicited cytotoxic action. A more prominent decrease in testosterone production (...
Veterinarni Medicina, Oct 15, 2022
Currently, in animal nutrition, the replacement of synthetic substances with natural ones was exp... more Currently, in animal nutrition, the replacement of synthetic substances with natural ones was expected to improve animal health. The aim of the present study was to evaluate the effects of a dietary brown seaweed and plant polyphenol mixture in adult male rabbits on the haematological profile and antioxidant markers. Twentyfour adult male rabbits were divided into three experimental groups receiving a control diet (C) or diets supplemented with 0.3% (T1) and 0.6% (T2) of a feed additive containing brown seaweed (Laminaria spp.) and plant extracts of seaweed origin. The trial lasted for 90 days. A lower potassium concentration was observed at 30 days in the T2 group, compared with the T1 and C groups. An increase in the antioxidant status was observed (P < 0.05) from day 60 of the trial in the rabbits fed diets with an algae-polyphenolic supplement (T1 and T2 groups). Concluding, the diet supplementation of brown seaweed and polyphenol stimulates the antioxidant status of the blood, however, it does not affect the haematological profile.
Biological Trace Element Research, Jul 3, 2018
The monitoring of various elements in the cow milk is important in the agricultural sector. The g... more The monitoring of various elements in the cow milk is important in the agricultural sector. The goal of this study was to determine the concentrations of calcium (Ca), cadmium (Cd), copper (Cu), iron (Fe), mercury (Hg), potassium (K), magnesium (Mg), sodium (Na), nickel (Ni), lead (Pb), and zinc (Zn) in the milk samples coming from different origins (local region in Nitra, nationwide sample represents the common Slovak brands of milk and common brands of milk from Czech Republic widely available in Slovakia). The samples were analyzed using atomic absorption spectrometry. A high level of variability in the Cu, Fe, Na, Ni, and Zn contents was observed. Magnesium was comparatively high in the milk sample from Slovakia (273.23 ± 16.32 μg/mL) against sample from Nitra (230.91 ± 9.48 μg/mL) and Czech Republic (202.70 ± 10.83 μg/mL). Potassium was significantly higher in milk from Nitra region (3301.98 ± 95.66) against SK sample (2925.16 ± 75.74 μg/mL). There were no significant differences in other elements among the three regions. The provisional tolerable weekly intake (PTWI) contribution suggested low dietary exposure to observed toxic metals (Cd, Hg, and Pb) in milk samples. Margin of exposure (MOE) evaluation denoted that even higher consumption of milk poses no high cardiovascular and nephrotoxicity threat. However, Cd and Pb are known for their cumulative effect and the monitoring of these elements in milk is strongly required.
Folia Biologica
The aim of this in vitro study was to examine the dose-dependent effects of iron as a potential e... more The aim of this in vitro study was to examine the dose-dependent effects of iron as a potential endocrine disruptor in relation to the release of sexual steroid hormones by a human adrenocortical carcinoma (NCI-H295R) cell line. The cells were exposed to different concentrations (3.90, 62.50, 250, 500, 1000 μM) of FeSO4.7H2O and compared with the control group (culture medium without FeSO4.7H2O). Cell viability was measured by the metabolic activity assay. Quantification of sexual steroid production was performed by enzyme-linked immunosorbent assay. Following 48 h culture of the cells in the presence of FeSO4.7H2O, significantly (P < 0.001) increased production of progesterone was observed at the lowest concentration (3.90 μM) of FeSO4.7H2O, whereas the lowest release of progesterone by NCIH295R cells was noted after addition of 1000 μM of FeSO4.7H2O, which did not elicit cytotoxic action (P > 0.05). Testosterone production was substantially increased at the concentrations ≤ ...
The purpose of this study was to monitor concentration of selected biological and risk elements i... more The purpose of this study was to monitor concentration of selected biological and risk elements in testes and later epididymal spermatozoa motility of wild boars (Sus scrofa scrofa) as well as their association. Wild boars were hunted in region Žuhračka - Levice branch plant, Slovak Republic. Testes were surgically removed post-mortem and were transported to the laboratory. Spermatozoa were obtained by dissecting the distal portion of the cauda epididymis and were analysed by Computer assisted semen analyzer (CASA) system. Concentration of elements were measured by inductively-coupled plasma optical emission spectrometry (ICP-OES) and by cold vapor absorption spectrometer (MA-3 Solo Mercury Analyzer). Total spermatozoa motility was at the level of 44.29% and progressive motility was 18.47%. Concentration of elements in testes was in following order: K > Na > Mg > Ca > Fe > Zn > Al > Cu > Se > Mn > As > Cr > Pb > Mo > Sr > Ni > Ba > ...
Toxics
Various studies have shown that the reproductive organs are highly sensitive to toxic elements fo... more Various studies have shown that the reproductive organs are highly sensitive to toxic elements found in the environment. Due to technological progress, the use of nanoparticles has become more common nowadays. Nanoparticles are used for drug delivery because their dimensions allow them to circulate throughout the body and enter directly into the cell. Antimicrobial properties are increasingly used in the manufacture of medical devices, textiles, food packaging, cosmetics, and other consumer products. Nanoparticles provide several benefits, but aspects related to their effects on living organisms and the environment are not well known. This review summarizes current in vivo, and in vitro animal studies focused on the evaluation of toxicity of selected metal nanoparticles (Ag, ZnO, TiO2) on male and female reproductive health. It can be concluded that higher concentrations of metal nanoparticles in the male reproductive system can cause a decrease in spermatozoa motility, viability an...
Journal of microbiology, biotechnology and food sciences
The objective of the present study was to investigate semen quality of infertile smokers and non-... more The objective of the present study was to investigate semen quality of infertile smokers and non-smokers including Benzo[a]Pyrene-7,8-diol-9,10 epoxide (BPDE)-DNA adduct formation and acrosome status of sperm cells. Semen quality parameters were analyzed according to 2010 World Health Organization criteria in 122 non-smokers and 125 cigarette smokers attending tertiary health care centre in Barak Valley of Assam, India followed by BPDE-DNA adduct formation and acrosome status test. A significant decline was found in sperm concentration, progressive motility, morphology in smokers (p
The Journal of Microbiology, Biotechnology and Food Sciences, Jan 6, 2021
The aim of this study was to determine the effect of dried strawberry leaves inclusion on selecte... more The aim of this study was to determine the effect of dried strawberry leaves inclusion on selected haematological parameters of rabbits: white blood cell, red blood cell, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, platelets, mean platelet volume, red cell distribution width, lymphocytes, mid cells total count, granulocytes, haematocrit, platelet distribution width. Thirtyrabbits of broiler line Californian were used in this experiment. The animals were divided into the four groups, four animals in each one (control group C and experimental groups E1, E2 and E3). Animals were fed ad libitum using KKV1 feeding mixture (FM) with or without strawberry leave inclusion as follows: group E1 received feed mixture with concentration of 0.5 % strawberry leaves group E2 1.0 % and group E3 1.5 %. Bloodwas collectedinto tubes and the parameters were analysed using Abacus Junior Vet. Statistical analyse showed a significant change (P <0.05) in mean platelet volume between group C and E1 and between group C and E2. Increase of this parameter was detected in all groups in comparison with the control group. Other haematological parameters were without significant differences (P>0.05). Strawberry leaves as a source of antioxidants altered haematological parameters according to our expectations. This study presents only partial results of continuing experiment.
Journal of Environmental Science and Health, Part A, 2021
Abstract The purpose of the present study was to evaluate the effects of aflatoxin B1 (AFB1) and ... more Abstract The purpose of the present study was to evaluate the effects of aflatoxin B1 (AFB1) and benzo[a]pyrene (BaP) on the heart muscle of chicken embryos of the broiler line Ross 308. The benzo[a]pyrene in the organic oil solution was injected in ovo on the 6th day of the incubation in doses of: 0.1, 0.5, and 1 mg/kg weight of eggs; the aflatoxin B1 in the organic oil solution was injected in ovo on the 6th day of the incubation into the yolk in doses of 80, 120 and 240 ng/kg weight of eggs. Multiple biochemical and hepatic parameters have been observed, including sodium, potassium, chloride, cholesterol, uric acid, total proteins, aminotransferase aspartate, and aminotransferase alanine. A low dose of AFB1 and BaP administered in ovo during early embryonic development had a significant impact on chicken embryonic development, as demonstrated by alterations in biochemical, mineral, and hepatic parameters.
The Journal of Microbiology, Biotechnology and Food Sciences, 2017
Acrylamide monomer causes neurotoxicity, male reproductive toxicity and endocrine – related tumor... more Acrylamide monomer causes neurotoxicity, male reproductive toxicity and endocrine – related tumors in rodents. Acrylamide was found in food, heat-processed potato products, coffee, bread and has been classified as probable human carcinogen by IARC. Daily intake from foodstuff was estimated in the range of 0.3-0.8 mg/kg bw per day (Seal et al., 2008). Glutathione peroxidase catalyzes the reduction hydrogen peroxide by glutathione. Extracellular superoxide dismutase catalyzes the dismutation of superoxide radical to hydrogen peroxide and oxygen. The activity of superoxide dismutase and glutathione peroxidase has been examined. The research was conducted on SWISS mice 8 week old, weight 26 g. Decapitation was carried out after 48, 72 and 192 hours after acrylamide injection. Animals received two doses of acrylamide 20 mg/kg and 40 mg/kg with intraperitoneal injection three times a week. Control group received physiological saline 3 times a week. The activity of superoxide dismutase was...
Universal Journal of Agricultural Research, 2014
In this study distribution of nickel as a risk factor of environment in testis and its effects on... more In this study distribution of nickel as a risk factor of environment in testis and its effects on the testicular structure in experimental animals and effect on spermatozoa was analyzed. In this study the effect of Ni on the testicular structure after an experimental intraperitoneal (i.p.) administration, concentration of nickel in semen of different animal species and the effect of in vitro spermatozoa incubation with nickel on the spermatozoa motility and membrane changes is reported. Our findings clearly suggest a negative effect of nickel on the structure as well as on the function of seminiferous epithelium. In experimental groups with nickel a significant (p 250 μM Ni/ml) cause decrease of spermatozoa motility in vitro.
Slovak Journal of Animal Science, 2014
In the present study the effect of collection regime on rabbit semen development was analyzed. Ei... more In the present study the effect of collection regime on rabbit semen development was analyzed. Eight seminal traits, such as overall ejaculate volume (VOL), spermatozoa concentration (CON), motility (MOT), progressive motility (PRO), percentage of progressive motility (% PRO), curvilinear velocity (VCL), beat-cross frequency (BCF) and amplitude of lateral head displacement (ALH) were analyzed. As the number of collections increased, more bucks were able to ejaculate. During collections the semen volume gradually increased from 0.4 ml to 1.0 ml with the average of 0.68 ± 0.34 ml. The average of MOT was 55.55 ± 26.63 % and the average of PRO reached 40.43 ± 27.35 %. The other assessed parameters were CON - 0.547 ± 0.165 x 10 9 ml -1 ; ALH - 4.18 ± 1.18 µm; BCF - 27.42 ± 7.12 Hz; VCL - 104.04 ± 37.00 µm.s -1 �� A positive correlation between ejaculate volume and the number of collections was found. MOT and PRO significantly correlated with the number of collections. In addition, positi...
The aim of this study was to observe the effect of age on selected biochemical parameters of hors... more The aim of this study was to observe the effect of age on selected biochemical parameters of horses' blood. Horses were divided in four age categories (5, 6, 7 and 9 years old). Blood was collected from 31 horses (Furioso breed). Assessment of biochemical parameters was realized by Randox RX Monza analyzer. Significant differences were observed between different groups in total proteins. Concentrations of calcium, phosphorus, sodium, potassium and chlorides correlate with the age of the animals. Similarly, liver enzyme activities correspond to age groups. In the other monitored biochemical parameters any significant differences between the groups were found. The achieved results confirm the connection between age of horses and values of biochemical parameters.
Slovak Journal of Animal Science, 2009
concentrations of cooper, zinc, iron, manganese, cadmium, lead and nickel in the semen of rabbits... more concentrations of cooper, zinc, iron, manganese, cadmium, lead and nickel in the semen of rabbits (n=27) in relation to spermato zoa quality were evaluated. samples were analyzed on an atomic absorption spectrophotometer (Unicam solar 939).
Spermatozoa - Facts and Perspectives, 2018
Oxidative stress (OS) is a condition caused by an imbalance between reactive oxygen species (ROS)... more Oxidative stress (OS) is a condition caused by an imbalance between reactive oxygen species (ROS) overgeneration and decreased antioxidant defense mechanisms in the cell. OS has become a prominent factor in male reproductive dysfunction as ROS cause damage to sperm DNA, lipids and proteins, alterations to critical sperm structures and signaling pathways, leading to a decreased sperm activity and fertilizing capacity. At the same time, small amounts of ROS play vital roles in events leading to sperm maturation and acquisition of functional activity, which is why a proper oxidative balance is of paramount importance for a proper male fertility. Understanding the physiological and pathological roles of ROS in male reproduction has become an essential pillar of modern andrology; however, numerous questions related to the controversial behavior of ROS in male reproductive cells and tissues still remain unanswered. This chapter aims to summarize current evidence available on the relationships between free radicals, antioxidants and male reproduction and to trigger more scientific interest, particularly with respect to the design of efficient strategies to diagnose or treat male sub-or infertility associated with OS.
Physiological Research, 2019
The target of this study was to evaluate the effect of extract of the European mistletoe – Viscum... more The target of this study was to evaluate the effect of extract of the European mistletoe – Viscum album quercus L. on spermatozoa motility and viability in vitro. The CASA system was used to determine the spermatozoa motility parameters at different time intervals (0, 1, 2 and 3 h) and spermatozoa viability was determined in five different doses of Viscum album quercus L [10 (QA), 6.6 (QB), 3.3 (QC), 2.5 (QD) and 2 (QE) mg/ml]. Results in experimental groups detected a significant deterioration on rabbit spermatozoa after 1, 2 and 3 hours, compared to the control. The initial total spermatozoa motility showed increased value for all doses of Viscum album quercus in comparison to control. After in vitro culture a dose–dependent decrease (QA: reduction of 69.7 %, QB: reduction of 40.9 %) was found. For the progressive spermatozoa most significant decrease (86.8 % for QA vs. 48.5 % for QB) was detected compared to the control after 3 hours of culture. Spermatozoa viability (MTT test) w...
Physiological Research, 2020
Nickel is a ubiquitous environmental pollutant, which has various effects on reproductive endocri... more Nickel is a ubiquitous environmental pollutant, which has various effects on reproductive endocrinology. In this study, human adrenocortical carcinoma (NCI-H295R) cell line was used as an in vitro biological model to study the effect of nickel chloride (NiCl2) on the viability and steroidogenesis. The cells were exposed to different concentrations (3.90; 7.80; 15.60; 31.20; 62.50; 125; 250 and 500 μM) of NiCl2 and compared with control group (culture medium without NiCl2). The cell viability was measured by the metabolic activity assay. Production of sexual steroid hormones was quantified by enzyme linked immunosorbent assay. Following 48 h culture of the cells in the presence of NiCl2 a dose-dependent depletion of progesterone release was observed even at the lower concentrations. In fact, lower levels of progesterone were detected in groups with higher doses (≥125 μM) of NiCl2 (P<0.01), which also elicited cytotoxic action. A more prominent decrease in testosterone production (...