Phan Thoa - Academia.edu (original) (raw)

Papers by Phan Thoa

Research paper thumbnail of BIM application for analyzing impacts of construction parameters on energy use and energy costs in buildings

Journal of Science and Technology in Civil Engineering (STCE) - NUCE

Energy-saving solutions in buildings in Vietnam are a great concern for sustainable development n... more Energy-saving solutions in buildings in Vietnam are a great concern for sustainable development nowadays. However, energy-efficient and cost-saving designs based on the integration of Building Information Modeling (BIM) and building energy analysis are still limited. This study aims to provide energy-efficient and cost-saving designs for buildings by performing the cloud-based building energy simulation. Particularly, the analysis of the impact of changing building parameters on energy consumption and energy cost was performed in this study. Considered building parameters includes building orientation, wall construction, window-to-wall ratio (WWR), lighting efficiency, daylighting and occupancy controls, and, heating ventilation, and air conditioning system. The findings of the study can facilitate building designers, building owners or investors can obtain the best solution for designing the buildings. The first contribution of the study, to provide an in-depth analysis of the impa...

Research paper thumbnail of Frequent Chlamydophila pneumoniae infection in vertically HIV-infected children in Vietnam

Journal of Reproductive Immunology, 2014

s for the 29th Annual Congress of Japanese Society / Journal of Reproductive Immunology 106 (2014... more s for the 29th Annual Congress of Japanese Society / Journal of Reproductive Immunology 106 (2014) 1–20 17 LPS stimulation induced a significant increase of transcript levels of matrix metalloproteinase (MMP)-1, MMP-3, and MMP-9, while P4 treatment significantly attenuated LPSinduced increase of transcript levels of these MMPs. Conclusions: Our preliminary results suggest that P4 might prevent spontaneous preterm labor by inhibiting cervical remodeling, particularly through the attenuation of ECM degradation by inhibiting MMPs production. http://dx.doi.org/10.1016/j.jri.2014.09.040 Analysis of miRNA–mRNA expression profiles in decidual natural killer cells during early pregnancy D. Zhao1,∗, B. Than Naing1, K. Inada2, T. Shima2, S. Saito2, T. Takeshita3, T. Takizawa1 1 Department of Molecular Medicine & Anatomy, Nippon Medical School, Tokyo, Japan 2 Department of Obstetrics and Gynecology, University of Toyama, Toyama, Japan 3 Department of Obstetrics and Gynecology, Nippon Medical School, Tokyo, Japan Objective: Decidual natural killer (dNK) cells play an important role in the maintenance of early pregnancy. However, the expression profiles and functions of miRNAs within dNK cells remain to be clarified.We investigated the miRNA–mRNAexpressionprofiles in dNK cells during early pregnancy. Methods:Decidual tissue and peripheral blood samples from patients who gave informed consent were aseptically obtained after legal abortions (at 6–7 weeks of gestation, n=3). We compared miRNA-mRNA expression patterns between dNK and peripheral NK (pNK) cells. The expression levels of miRNAs were examined by real-time PCR using a TaqMan microRNA Assay, and gene expression profiling was conducted using an Agilent microarray. Networks, functions, and pathways analyses were generated using Ingenuity Pathway Analysis (IPA). Results and conclusions: Among the 750 miRNAs examined, 199 miRNAs were significantly up-regulated in dNK cells compared with pNK cells. We detected some placenta-specific miRNAs derived from the chromosome 19 microRNA cluster in dNK cells. Among the 34,127 transcripts examined, 534 genes were significantly downregulated in dNK cells compared with pNK cells; the down-regulated gene expression profile exhibited low cytotoxicity in dNK cells. Some of the up-regulated miRNAs in dNK cells were the miRNAs potentially targeting the above cytotoxicity-associated genes. These data suggest that dNKcells have auniquemiRNA-mRNA interaction network for the maintenance of early pregnancy. http://dx.doi.org/10.1016/j.jri.2014.09.041 Frequent Chlamydophila pneumoniae infection in vertically HIV-infected children in Vietnam Q.D. Trinh ∗, N.T. Pham, N.T. Le Nguyen, B.Q. Lam, K.T. Le Phan, K.H. Truong, T.Q. Le, H.T. Nguyen, T.C. Tang, Y. Izumi, S. Komine-Aizawa, H. Ushijima, S. Hayakawa Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Japan Objective: Chlamydophila pneumoniae (C. pneumoniae) is an intracellular pathogen and often cause fatal respiratory symptoms in HIV/AIDS patients. However, its frequency in children with vertical HIV infection is so far unknown. Methods: A total of 89 blood samples collected from HIV-infected infants and children from provinces of southern Vietnam who were hospitalized at Children’s Hospital 1, Ho Chi Minh City, during the 1-year period fromOctober 2004 to September 2005 were submitted to serological screening for IgG, IgA, and IgM antibodies against C. pneumoniae. The presence of this microorganism was also evaluated by PCR. Age matched control enrolled HIV negative 91 children with other infectious disorders. Results: The results showed that 64% of the samples were positive for anti-C. pneumoniae IgG, 31.5% were positive for IgA, and 3.4% were positive for IgM. The highest prevalences of IgG and IgA positivity, 75% and 66.7%, respectively, were noted in the 1to 2-year-old age group. In control patients, IgG and IgA positivitywere 7.7 and 4.4% respectively. No patients showed positive PCR results. Conclusions: Our study revealed a high seroprevalence of C. pneumoniae in Vietnamese infants and children with HIV/AIDS by vertical transmission. http://dx.doi.org/10.1016/j.jri.2014.09.042 Porphyromonas gingivalis inhibits trophoblast invasion N. Hirohata ∗, S. Komine-Aizawa, S. Hayakawa Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Japan Objective: Periodontitis has been regarded as a risk factor of pregnancy complications including preterm birth, intrauterine growth retardation and preeclampsia. However protective roles of dental intervention during pregnancyon fetal/maternal outcomes are controversial. In this study, we investigated in vitro effects of a periodontal bacteria Porphyromonas gingivalis on cultured trophoblasts and mucosal natural killer cells to mimic in vivo phenomena at the feto-maternal interface. Methods: 2×109 of P. gingivalis (ATCC33277)were cultured at 37…

Research paper thumbnail of Subtyping and env C2/V3 Sequence Analysis of HIV-1 Isolated from HIV-Infected Children Hospitalized in Children Hospital 1, Vietnam during 2004-2005

Journal of Tropical Pediatrics, 2009

A molecular epidemiological study was conducted on 104 HIV-1 strains isolated from HIV-infected c... more A molecular epidemiological study was conducted on 104 HIV-1 strains isolated from HIV-infected children hospitalized in Children Hospital 1 in Ho Chi Minh City, Vietnam during 2004-2005. Genetic subtyping based on env C2/V3 sequences revealed that CRF01-AE was the sole circulating recombinant form found in this study. Sequence analysis of the V3 loop showed that GPGQ tetramer was the most common V3 loop core motif identified in the HIV-1 strains studied (89.5%). The findings raise great concern about HIV-infected children in Vietnam and provide up-to-date molecular epidemiological information of HIV-1 circulating in Vietnam during the study period.

Research paper thumbnail of Seroprevalence of Chlamydophila pneumoniae in HIV-infected children in Vietnam

Journal of Infection and Chemotherapy, 2013

Analysis of rearrangement of HBV integration in patients with HCC Shunichi MATSUOKA et. al 1 Deve... more Analysis of rearrangement of HBV integration in patients with HCC Shunichi MATSUOKA et. al 1 Development of anti-cancer therapy using non-thermal atmospheric pressure plasma Masayoshi SOMA et. al 9 Establishment of new therapy which targets mast cells in rheumatoid arthritis and chronic urticaria Yoshimichi OKAYAMA et. al 15

Research paper thumbnail of Short Communication: Drug Resistance Mutations in the HIV Type 1 Protease and Reverse Transcriptase Genes in Antiretroviral-Naive Vietnamese Children

AIDS Research and Human Retroviruses, 2012

Anti-HIV drugs have recently become available for the treatment of children infected with HIV in ... more Anti-HIV drugs have recently become available for the treatment of children infected with HIV in Vietnam; however, the genetic background of HIV-1 drug resistance in antiretroviral-naive children has yet to be studied. Of the 104 HIV-1 CRF01-AE subtype strains that were previously isolated from antiretroviral-naive children from the provinces of southern Vietnam and hospitalized in Children Hospital 1 in Ho Chi Minh City from 2004 to 2005, 79 strains were used for amplification and sequence analyses of the protease and reverse transcriptase (RT) genes. Minor mutations were found in the protease gene, including L10I, I13V, G16E, M36I, D60E, I62V, I64V, L63P, H69K, V82I, and I93L. Of these mutations, M36I and H69K were detected in all of the strains that were studied. However, all of the amino acid changes in the protease gene were considered to be polymorphisms. In the RT gene, three major mutations were detected in six strains: the V75M mutation in one strain, the Y181C mutation in two strains, and the M184I mutation in three strains. The prevalence of primary or transmitted HIV drug resistance to all of the drugs and drug classes that were evaluated in this study was 7.6%. These findings provide a useful background for antiretroviral therapy in Vietnam and contribute reference data for the surveillance of HIV drug resistance around the world. This study suggests that the prevalence of HIVDR in Vietnam may have recently increased. The monitoring of HIV drug resistance in Vietnam is necessary.

Research paper thumbnail of BIM application for analyzing impacts of construction parameters on energy use and energy costs in buildings

Journal of Science and Technology in Civil Engineering (STCE) - NUCE

Energy-saving solutions in buildings in Vietnam are a great concern for sustainable development n... more Energy-saving solutions in buildings in Vietnam are a great concern for sustainable development nowadays. However, energy-efficient and cost-saving designs based on the integration of Building Information Modeling (BIM) and building energy analysis are still limited. This study aims to provide energy-efficient and cost-saving designs for buildings by performing the cloud-based building energy simulation. Particularly, the analysis of the impact of changing building parameters on energy consumption and energy cost was performed in this study. Considered building parameters includes building orientation, wall construction, window-to-wall ratio (WWR), lighting efficiency, daylighting and occupancy controls, and, heating ventilation, and air conditioning system. The findings of the study can facilitate building designers, building owners or investors can obtain the best solution for designing the buildings. The first contribution of the study, to provide an in-depth analysis of the impa...

Research paper thumbnail of Frequent Chlamydophila pneumoniae infection in vertically HIV-infected children in Vietnam

Journal of Reproductive Immunology, 2014

s for the 29th Annual Congress of Japanese Society / Journal of Reproductive Immunology 106 (2014... more s for the 29th Annual Congress of Japanese Society / Journal of Reproductive Immunology 106 (2014) 1–20 17 LPS stimulation induced a significant increase of transcript levels of matrix metalloproteinase (MMP)-1, MMP-3, and MMP-9, while P4 treatment significantly attenuated LPSinduced increase of transcript levels of these MMPs. Conclusions: Our preliminary results suggest that P4 might prevent spontaneous preterm labor by inhibiting cervical remodeling, particularly through the attenuation of ECM degradation by inhibiting MMPs production. http://dx.doi.org/10.1016/j.jri.2014.09.040 Analysis of miRNA–mRNA expression profiles in decidual natural killer cells during early pregnancy D. Zhao1,∗, B. Than Naing1, K. Inada2, T. Shima2, S. Saito2, T. Takeshita3, T. Takizawa1 1 Department of Molecular Medicine & Anatomy, Nippon Medical School, Tokyo, Japan 2 Department of Obstetrics and Gynecology, University of Toyama, Toyama, Japan 3 Department of Obstetrics and Gynecology, Nippon Medical School, Tokyo, Japan Objective: Decidual natural killer (dNK) cells play an important role in the maintenance of early pregnancy. However, the expression profiles and functions of miRNAs within dNK cells remain to be clarified.We investigated the miRNA–mRNAexpressionprofiles in dNK cells during early pregnancy. Methods:Decidual tissue and peripheral blood samples from patients who gave informed consent were aseptically obtained after legal abortions (at 6–7 weeks of gestation, n=3). We compared miRNA-mRNA expression patterns between dNK and peripheral NK (pNK) cells. The expression levels of miRNAs were examined by real-time PCR using a TaqMan microRNA Assay, and gene expression profiling was conducted using an Agilent microarray. Networks, functions, and pathways analyses were generated using Ingenuity Pathway Analysis (IPA). Results and conclusions: Among the 750 miRNAs examined, 199 miRNAs were significantly up-regulated in dNK cells compared with pNK cells. We detected some placenta-specific miRNAs derived from the chromosome 19 microRNA cluster in dNK cells. Among the 34,127 transcripts examined, 534 genes were significantly downregulated in dNK cells compared with pNK cells; the down-regulated gene expression profile exhibited low cytotoxicity in dNK cells. Some of the up-regulated miRNAs in dNK cells were the miRNAs potentially targeting the above cytotoxicity-associated genes. These data suggest that dNKcells have auniquemiRNA-mRNA interaction network for the maintenance of early pregnancy. http://dx.doi.org/10.1016/j.jri.2014.09.041 Frequent Chlamydophila pneumoniae infection in vertically HIV-infected children in Vietnam Q.D. Trinh ∗, N.T. Pham, N.T. Le Nguyen, B.Q. Lam, K.T. Le Phan, K.H. Truong, T.Q. Le, H.T. Nguyen, T.C. Tang, Y. Izumi, S. Komine-Aizawa, H. Ushijima, S. Hayakawa Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Japan Objective: Chlamydophila pneumoniae (C. pneumoniae) is an intracellular pathogen and often cause fatal respiratory symptoms in HIV/AIDS patients. However, its frequency in children with vertical HIV infection is so far unknown. Methods: A total of 89 blood samples collected from HIV-infected infants and children from provinces of southern Vietnam who were hospitalized at Children’s Hospital 1, Ho Chi Minh City, during the 1-year period fromOctober 2004 to September 2005 were submitted to serological screening for IgG, IgA, and IgM antibodies against C. pneumoniae. The presence of this microorganism was also evaluated by PCR. Age matched control enrolled HIV negative 91 children with other infectious disorders. Results: The results showed that 64% of the samples were positive for anti-C. pneumoniae IgG, 31.5% were positive for IgA, and 3.4% were positive for IgM. The highest prevalences of IgG and IgA positivity, 75% and 66.7%, respectively, were noted in the 1to 2-year-old age group. In control patients, IgG and IgA positivitywere 7.7 and 4.4% respectively. No patients showed positive PCR results. Conclusions: Our study revealed a high seroprevalence of C. pneumoniae in Vietnamese infants and children with HIV/AIDS by vertical transmission. http://dx.doi.org/10.1016/j.jri.2014.09.042 Porphyromonas gingivalis inhibits trophoblast invasion N. Hirohata ∗, S. Komine-Aizawa, S. Hayakawa Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Japan Objective: Periodontitis has been regarded as a risk factor of pregnancy complications including preterm birth, intrauterine growth retardation and preeclampsia. However protective roles of dental intervention during pregnancyon fetal/maternal outcomes are controversial. In this study, we investigated in vitro effects of a periodontal bacteria Porphyromonas gingivalis on cultured trophoblasts and mucosal natural killer cells to mimic in vivo phenomena at the feto-maternal interface. Methods: 2×109 of P. gingivalis (ATCC33277)were cultured at 37…

Research paper thumbnail of Subtyping and env C2/V3 Sequence Analysis of HIV-1 Isolated from HIV-Infected Children Hospitalized in Children Hospital 1, Vietnam during 2004-2005

Journal of Tropical Pediatrics, 2009

A molecular epidemiological study was conducted on 104 HIV-1 strains isolated from HIV-infected c... more A molecular epidemiological study was conducted on 104 HIV-1 strains isolated from HIV-infected children hospitalized in Children Hospital 1 in Ho Chi Minh City, Vietnam during 2004-2005. Genetic subtyping based on env C2/V3 sequences revealed that CRF01-AE was the sole circulating recombinant form found in this study. Sequence analysis of the V3 loop showed that GPGQ tetramer was the most common V3 loop core motif identified in the HIV-1 strains studied (89.5%). The findings raise great concern about HIV-infected children in Vietnam and provide up-to-date molecular epidemiological information of HIV-1 circulating in Vietnam during the study period.

Research paper thumbnail of Seroprevalence of Chlamydophila pneumoniae in HIV-infected children in Vietnam

Journal of Infection and Chemotherapy, 2013

Analysis of rearrangement of HBV integration in patients with HCC Shunichi MATSUOKA et. al 1 Deve... more Analysis of rearrangement of HBV integration in patients with HCC Shunichi MATSUOKA et. al 1 Development of anti-cancer therapy using non-thermal atmospheric pressure plasma Masayoshi SOMA et. al 9 Establishment of new therapy which targets mast cells in rheumatoid arthritis and chronic urticaria Yoshimichi OKAYAMA et. al 15

Research paper thumbnail of Short Communication: Drug Resistance Mutations in the HIV Type 1 Protease and Reverse Transcriptase Genes in Antiretroviral-Naive Vietnamese Children

AIDS Research and Human Retroviruses, 2012

Anti-HIV drugs have recently become available for the treatment of children infected with HIV in ... more Anti-HIV drugs have recently become available for the treatment of children infected with HIV in Vietnam; however, the genetic background of HIV-1 drug resistance in antiretroviral-naive children has yet to be studied. Of the 104 HIV-1 CRF01-AE subtype strains that were previously isolated from antiretroviral-naive children from the provinces of southern Vietnam and hospitalized in Children Hospital 1 in Ho Chi Minh City from 2004 to 2005, 79 strains were used for amplification and sequence analyses of the protease and reverse transcriptase (RT) genes. Minor mutations were found in the protease gene, including L10I, I13V, G16E, M36I, D60E, I62V, I64V, L63P, H69K, V82I, and I93L. Of these mutations, M36I and H69K were detected in all of the strains that were studied. However, all of the amino acid changes in the protease gene were considered to be polymorphisms. In the RT gene, three major mutations were detected in six strains: the V75M mutation in one strain, the Y181C mutation in two strains, and the M184I mutation in three strains. The prevalence of primary or transmitted HIV drug resistance to all of the drugs and drug classes that were evaluated in this study was 7.6%. These findings provide a useful background for antiretroviral therapy in Vietnam and contribute reference data for the surveillance of HIV drug resistance around the world. This study suggests that the prevalence of HIVDR in Vietnam may have recently increased. The monitoring of HIV drug resistance in Vietnam is necessary.