Pie Huda - Academia.edu (original) (raw)
Uploads
Papers by Pie Huda
PLOS ONE, 2015
Cu radiolabelled nanodiscs based on the 11 α-helix MSP1E3D1 protein and 1-palmitoyl-2-oleoyl-sn-g... more Cu radiolabelled nanodiscs based on the 11 α-helix MSP1E3D1 protein and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine lipids were, for the first time, followed in vivo by positron emission tomography for evaluating the biodistribution of nanodiscs. A cancer tumor bearing mouse model was used for the investigations, and it was found that the approximately 13 nm nanodiscs, due to their size, permeate deeply into cancer tissue. This makes them promising candidates for both drug delivery purposes and as advanced imaging agents. For the radiolabelling, a simple approach for 64 Cu radiolabelling of proteins via a chelating agent, DOTA, was developed. The reaction was performed at sufficiently mild conditions to be compatible with labelling of the protein part of a lipid-protein particle while fully conserving the particle structure including the amphipathic protein fold. Fig 1. Schematics of nanodisc radiolabelling. A: chemical reaction of DOTA-NHS ester-conjugation to MSP lysines and further radiolabelling with 64 Cu 2+ . B: symbol explanation for C. C 1: MD simulation of ordinary nanodisc[6] (lipids in blue, MSP in red). 2: nanodisc assembled with DOTA conjugated MSP. 3: 64 Cu radiolabelled nanodisc.
PLOS ONE, 2015
Cu radiolabelled nanodiscs based on the 11 α-helix MSP1E3D1 protein and 1-palmitoyl-2-oleoyl-sn-g... more Cu radiolabelled nanodiscs based on the 11 α-helix MSP1E3D1 protein and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine lipids were, for the first time, followed in vivo by positron emission tomography for evaluating the biodistribution of nanodiscs. A cancer tumor bearing mouse model was used for the investigations, and it was found that the approximately 13 nm nanodiscs, due to their size, permeate deeply into cancer tissue. This makes them promising candidates for both drug delivery purposes and as advanced imaging agents. For the radiolabelling, a simple approach for 64 Cu radiolabelling of proteins via a chelating agent, DOTA, was developed. The reaction was performed at sufficiently mild conditions to be compatible with labelling of the protein part of a lipid-protein particle while fully conserving the particle structure including the amphipathic protein fold. Fig 1. Schematics of nanodisc radiolabelling. A: chemical reaction of DOTA-NHS ester-conjugation to MSP lysines and further radiolabelling with 64 Cu 2+ . B: symbol explanation for C. C 1: MD simulation of ordinary nanodisc[6] (lipids in blue, MSP in red). 2: nanodisc assembled with DOTA conjugated MSP. 3: 64 Cu radiolabelled nanodisc.