Pierre Bilodeau - Academia.edu (original) (raw)
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Papers by Pierre Bilodeau
In 1986, it was shown that tobacco plants and sunflower calluses could express recombinant human ... more In 1986, it was shown that tobacco plants and sunflower calluses could express recombinant human growth hormone as a fusion protein [1]. Since then, a diverse range of plant systems has been used for the production of pharmaceuticals [2, 3]. We have developed a production system based on the leaves of alfalfa (Medicago
Plant-Made Pharmaceuticals and Technical Proteins, 2004
Proceedings of the National Academy of Sciences, 1999
We have cloned two genes, FIS1 and FIS2 , that control both fertilization independent seed develo... more We have cloned two genes, FIS1 and FIS2 , that control both fertilization independent seed development and postpollination embryo development in Arabidopsis . These genes confer female gametophytic phenotypes. FIS2 encodes a protein with a C 2 H 2 zinc-finger motif and three putative nuclear localization signals, indicating that it is likely to be a transcription factor. FIS1 encodes a protein with homology to the Drosophila Polycomb group gene Enhancer-of-zeste and is identical to the recently described Arabidopsis gene MEDEA . FIS1 is a protein with a number of putative functional domains, including the SET domain present in Enhancer-of-zeste-related proteins. Comparison of the position of the lesions in the fis1 and medea mutant alleles indicates that fis1 is a null allele producing a truncated polypeptide lacking all the protein domains whereas the deduced protein from medea lacks only the SET domain. We present a model of the role of FIS1 and FIS2 gene products in seed developm...
Plant, Cell and Environment, 1999
Proceedings of the National Academy of Sciences, 2000
The promoters of MEA ( FIS1 ) , FIS2, and FIE ( FIS3 ), genes that repress seed development in th... more The promoters of MEA ( FIS1 ) , FIS2, and FIE ( FIS3 ), genes that repress seed development in the absence of pollination, were fused to β-glucuronidase ( GUS ) to study their activity pattern. The FIS2 ∷ GUS product is found in the embryo sac, in each of the polar cell nuclei, and in the central cell nucleus. After pollination, the maternally derived FIS2 ∷ GUS protein occurs in the nuclei of the cenocytic endosperm. Before cellularization of the endosperm, activity is terminated in the micropylar and central nuclei of the endosperm and subsequently in the nuclei of the chalazal cyst. MEA ∷ GUS has a pattern of activity similar to that of FIS2 ∷ GUS , but FIE ∷ GUS protein is found in many tissues, including the prepollination embryo sac, and in embryo and endosperm postpollination. The similarity in mutant phenotypes; the activity of FIE , MEA, and FIS2 in the same cells in the embryo sac; and the fact that MEA and FIE proteins interact in a yeast two-hybrid system suggest that th...
Journal of Pest Science
Early intervention, effective management, and regulations are essential to mitigate the potential... more Early intervention, effective management, and regulations are essential to mitigate the potential negative impacts of invasive forest insects. Biosurveillance provides the necessary knowledge to inform management, and regulatory practices. Genomic approaches can contribute valuable information to this process. Unfortunately, adoption and incorporation of genomic tools into biosurveillance frameworks is not straightforward. To realize the full potential of genomic knowledge, researchers must work together with end users to ensure full adoption, standardization, validation, and interpretation of genomic results. Keywords Biological invasion · Invasive · HTS · Regulation · Policy · Surveillance Key message • We bring an end user perspective to the application of genomic tools for the management of invasive species. • We highlight the benefit of genomics to invasive species management, as well as the potential barriers to its adoption , and policy considerations for implementing genom-ics within a management and regulatory framework. • We present Biosurveillance of Alien Forest Enemies (bioSAFE) as a model for how to engage with the end user community at all stages of project development; from inception to adoption.
range of plant systems has been used for the production of pharmaceuticals (2, 3). We have develo... more range of plant systems has been used for the production of pharmaceuticals (2, 3). We have developed a production system based on the leaves of alfalfa (Medicago sativa L.), a choice made originally because of the plant's many favorable agronomic characteristics. Alfalfa is a perennial plant, so vegetative growth can be maintained for many years. For molecular farming, this characteristic,
Seminars in Cell & Developmental Biology, 1996
Plant Cell Reports, 1994
Cruciferin is the major seed storage protein in Brassica napus. As much as 1.9 kbp of the BnC1 cr... more Cruciferin is the major seed storage protein in Brassica napus. As much as 1.9 kbp of the BnC1 cruciferin gene promoter have been sequenced and analyzed. Promoter fragments with 5' deletions from -2500 to -v202 were fused with the ß-glucuronidase reporter gene and used for Nicotiana tabacum transformation. ß-glucuronidase could be specifically expressed in transgenic tobacco seeds under the control of the BnC1 promoter and regulatory elements were found to be dispersed over 1903 bp. An almost 5-fold increase in ß-glucuronidase expression was obtained when the promoter length was increased from -379 to -498, and another 10-fold increase was observed when sequences between -1266 and -1903 were added. Histochemical analysis shows that the region between -844 and -1266 directs the expression of the chimeric gene specifically to the root apical meristem.
Cruciferin is the major seed storage protein in Brassica napus. As much as 1.9 kbp of the BnC1 cr... more Cruciferin is the major seed storage protein in Brassica napus. As much as 1.9 kbp of the BnC1 cruciferin gene promoter have been sequenced and analyzed. Promoter fragments with 5' deletions from -2500 to -v202 were fused with the ß-glucuronidase reporter gene and used for Nicotiana tabacum transformation. ß-glucuronidase could be specifically expressed in transgenic tobacco seeds under the control of the BnC1 promoter and regulatory elements were found to be dispersed over 1903 bp. An almost 5-fold increase in ß-glucuronidase expression was obtained when the promoter length was increased from -379 to -498, and another 10-fold increase was observed when sequences between -1266 and -1903 were added. Histochemical analysis shows that the region between -844 and -1266 directs the expression of the chimeric gene specifically to the root apical meristem.
In 1986, it was shown that tobacco plants and sunflower calluses could express recombinant human ... more In 1986, it was shown that tobacco plants and sunflower calluses could express recombinant human growth hormone as a fusion protein [1]. Since then, a diverse range of plant systems has been used for the production of pharmaceuticals [2, 3]. We have developed a production system based on the leaves of alfalfa (Medicago
Plant-Made Pharmaceuticals and Technical Proteins, 2004
Proceedings of the National Academy of Sciences, 1999
We have cloned two genes, FIS1 and FIS2 , that control both fertilization independent seed develo... more We have cloned two genes, FIS1 and FIS2 , that control both fertilization independent seed development and postpollination embryo development in Arabidopsis . These genes confer female gametophytic phenotypes. FIS2 encodes a protein with a C 2 H 2 zinc-finger motif and three putative nuclear localization signals, indicating that it is likely to be a transcription factor. FIS1 encodes a protein with homology to the Drosophila Polycomb group gene Enhancer-of-zeste and is identical to the recently described Arabidopsis gene MEDEA . FIS1 is a protein with a number of putative functional domains, including the SET domain present in Enhancer-of-zeste-related proteins. Comparison of the position of the lesions in the fis1 and medea mutant alleles indicates that fis1 is a null allele producing a truncated polypeptide lacking all the protein domains whereas the deduced protein from medea lacks only the SET domain. We present a model of the role of FIS1 and FIS2 gene products in seed developm...
Plant, Cell and Environment, 1999
Proceedings of the National Academy of Sciences, 2000
The promoters of MEA ( FIS1 ) , FIS2, and FIE ( FIS3 ), genes that repress seed development in th... more The promoters of MEA ( FIS1 ) , FIS2, and FIE ( FIS3 ), genes that repress seed development in the absence of pollination, were fused to β-glucuronidase ( GUS ) to study their activity pattern. The FIS2 ∷ GUS product is found in the embryo sac, in each of the polar cell nuclei, and in the central cell nucleus. After pollination, the maternally derived FIS2 ∷ GUS protein occurs in the nuclei of the cenocytic endosperm. Before cellularization of the endosperm, activity is terminated in the micropylar and central nuclei of the endosperm and subsequently in the nuclei of the chalazal cyst. MEA ∷ GUS has a pattern of activity similar to that of FIS2 ∷ GUS , but FIE ∷ GUS protein is found in many tissues, including the prepollination embryo sac, and in embryo and endosperm postpollination. The similarity in mutant phenotypes; the activity of FIE , MEA, and FIS2 in the same cells in the embryo sac; and the fact that MEA and FIE proteins interact in a yeast two-hybrid system suggest that th...
Journal of Pest Science
Early intervention, effective management, and regulations are essential to mitigate the potential... more Early intervention, effective management, and regulations are essential to mitigate the potential negative impacts of invasive forest insects. Biosurveillance provides the necessary knowledge to inform management, and regulatory practices. Genomic approaches can contribute valuable information to this process. Unfortunately, adoption and incorporation of genomic tools into biosurveillance frameworks is not straightforward. To realize the full potential of genomic knowledge, researchers must work together with end users to ensure full adoption, standardization, validation, and interpretation of genomic results. Keywords Biological invasion · Invasive · HTS · Regulation · Policy · Surveillance Key message • We bring an end user perspective to the application of genomic tools for the management of invasive species. • We highlight the benefit of genomics to invasive species management, as well as the potential barriers to its adoption , and policy considerations for implementing genom-ics within a management and regulatory framework. • We present Biosurveillance of Alien Forest Enemies (bioSAFE) as a model for how to engage with the end user community at all stages of project development; from inception to adoption.
range of plant systems has been used for the production of pharmaceuticals (2, 3). We have develo... more range of plant systems has been used for the production of pharmaceuticals (2, 3). We have developed a production system based on the leaves of alfalfa (Medicago sativa L.), a choice made originally because of the plant's many favorable agronomic characteristics. Alfalfa is a perennial plant, so vegetative growth can be maintained for many years. For molecular farming, this characteristic,
Seminars in Cell & Developmental Biology, 1996
Plant Cell Reports, 1994
Cruciferin is the major seed storage protein in Brassica napus. As much as 1.9 kbp of the BnC1 cr... more Cruciferin is the major seed storage protein in Brassica napus. As much as 1.9 kbp of the BnC1 cruciferin gene promoter have been sequenced and analyzed. Promoter fragments with 5' deletions from -2500 to -v202 were fused with the ß-glucuronidase reporter gene and used for Nicotiana tabacum transformation. ß-glucuronidase could be specifically expressed in transgenic tobacco seeds under the control of the BnC1 promoter and regulatory elements were found to be dispersed over 1903 bp. An almost 5-fold increase in ß-glucuronidase expression was obtained when the promoter length was increased from -379 to -498, and another 10-fold increase was observed when sequences between -1266 and -1903 were added. Histochemical analysis shows that the region between -844 and -1266 directs the expression of the chimeric gene specifically to the root apical meristem.
Cruciferin is the major seed storage protein in Brassica napus. As much as 1.9 kbp of the BnC1 cr... more Cruciferin is the major seed storage protein in Brassica napus. As much as 1.9 kbp of the BnC1 cruciferin gene promoter have been sequenced and analyzed. Promoter fragments with 5' deletions from -2500 to -v202 were fused with the ß-glucuronidase reporter gene and used for Nicotiana tabacum transformation. ß-glucuronidase could be specifically expressed in transgenic tobacco seeds under the control of the BnC1 promoter and regulatory elements were found to be dispersed over 1903 bp. An almost 5-fold increase in ß-glucuronidase expression was obtained when the promoter length was increased from -379 to -498, and another 10-fold increase was observed when sequences between -1266 and -1903 were added. Histochemical analysis shows that the region between -844 and -1266 directs the expression of the chimeric gene specifically to the root apical meristem.