Pooja Bhardwaj - Academia.edu (original) (raw)

Papers by Pooja Bhardwaj

International Journal of Molecular Sciences

The early management, diagnosis, and treatment of emerging and re-emerging infections and the ris... more The early management, diagnosis, and treatment of emerging and re-emerging infections and the rising burden of non-communicable diseases (NCDs) are necessary. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas system has recently acquired popularity as a diagnostic tool due to its ability to target specific genes. It uses Cas enzymes and a guide RNA (gRNA) to cleave target DNA or RNA. The discovery of collateral cleavage in CRISPR-Cas effectors such as Cas12a and Cas13a was intensively repurposed for the development of instrument-free, sensitive, precise and rapid point-of-care diagnostics. CRISPR/Cas demonstrated proficiency in detecting non-nucleic acid targets including protein, analyte, and hormones other than nucleic acid. CRISPR/Cas effectors can provide multiple detections simultaneously. The present review highlights the technical challenges of integrating CRISPR/Cas technology into the onsite assessment of clinical and other specimens, along with cur...

Indian Journal of Chemical Technology

Advance inorganic fly ashmetakaolin-phosphatic geopolymer has been developed via greener approach... more Advance inorganic fly ashmetakaolin-phosphatic geopolymer has been developed via greener approach and deposited on mild steel substrate using spin-coating method, to improve the substrate-matrix interaction. The present research investigates the effect of incorporation of metakaolin and phosphate to geopolymer matrix to assess the performance of coating and optimization of spin coating parameters are performed as well to achieve maximum adhesion strength. XRD, FTIR, and SEM studies coupled with energy dispersive spectroscopy (EDX) have been carried out for determination of mineralogical phases, bonding interactions, substrate-matrix interface, and microstructural and chemical analysis.Results indicated that spin coating method produced well-ordered thin geopolymeric coating (thickness 13-20 micrometres), therefore spin coating deposition is the simplest way to obtain thin coatings of visibly thick materials. Maximum adhesion strength of material with substrate is 2.5M.Pa. which is obtained at optimized water to precursor ratio 0.5 at 500rpm spinning speed. It is concluded that new inorganic phases (originated from mutual contribution of phosphate, metakaolin and fly ash) like sodium iron aluminium phosphate, aluminium phosphate and sodium iron phosphate were responsible for good adhesion of material to the substrate and then related properties. The developed and optimized method can be used to produce homogeneous coating of heterogeneous geopolymer material on low carbon steel to indorse its advanced applications.

Rasayan Journal of Chemistry

The present work focuses on the growth of ZnO films vertically on indium tin oxide substrate usin... more The present work focuses on the growth of ZnO films vertically on indium tin oxide substrate using a wet chemical method. The morphological and structural study of the prepared films confirmed the formation of one-dimensional growth with a polycrystalline single-phase hexagonal wurtzite structure. The computed texture coefficient indicates the c-axis growth of the film. The optical spectra study exhibited a strong and sharp characteristics absorption band. The numerical derivative method and the Tauc plot method for the different transition values have been used to compare energy bandgap calculations.

Plant Disease Research, 2018

IJCT Vol.25(5) [September 2018], Sep 1, 2018

468-474In the present studies, three different compositions of geopolymeric coating material have... more 468-474In the present studies, three different compositions of geopolymeric coating material have been developed utilizing fly ash, red mud, alkali activators and sodium dihydrogen phosphate. TiO2 and Fe2O3 nanoparticles are added to the developed coating material compositions as additives. These compositions are applied to mild steel plates by spray coating technique. Further, adhesive strength, water stability and anti-corrosion behavior of geopolymeric coated mild steel plates are determined. Developed geopolymeric coating material compositions are characterized for minerology, bonding behavior and morphology by XRD, FTIR and SEM respectively. Results revealed that among all the three coating material compositions, composition containing nano TiO2 exhibited best performance in terms of adhesive strength [3.1 MPa at 7th day], water resistance and corrosion resistance characteristics in 3.5 weight % sodium chloride solution by electrochemical measurements

Clustered, Regularly Interspaced Short Palindromic Repeats and their associated Cas pro-teins (CR... more Clustered, Regularly Interspaced Short Palindromic Repeats and their associated Cas pro-teins (CRISPR-Cas) provide prokaryotes with a mechanism for defense against mobile genetic elements (MGEs). A CRISPR locus is a molecular memory of MGE encounters. It contains an array of short sequences, called spacers, that generally have sequence identity to MGEs. Three different CRISPR loci have been identified among strains of the opportunis-tic pathogen Enterococcus faecalis. CRISPR1 and CRISPR3 are associated with the cas genes necessary for blocking MGEs, but these loci are present in only a subset of E. faecalis strains. The orphan CRISPR2 lacks cas genes and is ubiquitous in E. faecalis, although its spacer content varies from strain to strain. Because CRISPR2 is a variable locus occurring in all E. faecalis, comparative analysis of CRISPR2 sequences may provide information about the clonality of E. faecalis strains. We examined CRISPR2 sequences from 228 E. faecalis genomes in relation...

B-cell exihibit B cell receptor or BCR, a transmembrane protein located on its surface. BCR compr... more B-cell exihibit B cell receptor or BCR, a transmembrane protein located on its surface. BCR comprises of two transmembrane proteins Igα and Igβ. Igβ (CD79B) is encoded by B29 gene. Leukaemic B-cells express low toundetectable Igs, Igβ (CD79B) proteins. Altered and aberrated B29 gene affect expression of Igβ/CD79B protein. One of the main factor of B29 gene alteration is mutation in their coding sequence. We analyzed the quantitative expression of B29 gene in leukaemic samples. Primers for B cell expression was designed using PrimerExpress 3.0 software. Designed primers were used to analyze the expression of B29 gene in different samples through ABI prism 7000 SDS system. Relative quantification of B29 gene in samples was done by 2^-ΔΔCt method. There was under expression of B29 gene in 100% of leukaemic samples. Though reason for lower gene expression in leukaemic samples was not detected yet there are number of papers reporting mutations and methylation as main cause of B29 gen alt...

Epidemiology and Infection, 2021

We report a familial cluster of 24 individuals infected with severe acute respiratory syndrome-co... more We report a familial cluster of 24 individuals infected with severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). The index case had a travel history and spent 24 days in the house before being tested and was asymptomatic. Physical overcrowding in the house provided a favourable environment for intra-cluster infection transmission. Restriction of movement of family members due to countrywide lockdown limited the spread in community. Among the infected, only four individuals developed symptoms. The complete genome sequences of SARS-CoV-2 was retrieved using next-generation sequencing from eight clinical samples which demonstrated a 99.99% similarity with reference to Wuhan strain and the phylogenetic analysis demonstrated a distinct cluster, lying in the B.6.6 pangolin lineage.

Environmental Quality Management, 2021

international journal of engineering trends and technology, 2014

B-cell exihibit B cell receptor or BCR, a transmembrane protein located on its surface. BCR compr... more B-cell exihibit B cell receptor or BCR, a transmembrane protein located on its surface. BCR comprises of two transmembrane proteins Igα and Igβ. Igβ (CD79B) is encoded by B29 gene. Leukaemic Bcells express low toundetectable Igs, Igβ (CD79B) proteins. Altered and aberrated B29 gene affect expression of Igβ/CD79B protein. One of the main factor of B29 gene alteration is mutation in their coding sequence. We analyzed the quantitative expression of B29 gene in leukaemic samples. Primers for B cell expression was designed using PrimerExpress 3.0 software. Designed primers were used to analyze the expression of B29 gene in different samples through ABI prism 7000 SDS system. Relative quantification of B29 gene in samples was done by 2^-ΔΔCt method. There was under expression of B29 gene in 100% of leukaemic samples. Though reason for lower gene expression in leukaemic samples was not detected yet there are number of papers reporting mutations and methylation as main cause of B29 gen alte...

Journal of the Practice of Cardiovascular Sciences, 2020

Objective: The coronavirus disease 2019 (COVID-19) also known as COVID-19 or severe acute respira... more Objective: The coronavirus disease 2019 (COVID-19) also known as COVID-19 or severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a viral infection caused by severe acute respiratory syndrome coronavirus 2, emerged first in Wuhan, China, and spread all over the world. The World Health Organization on January 30, 2020, officially declared the COVID-19 epidemic as a public health emergency of international concern. There is speculation as to the origin of the infection and the objective of this narrative review is to scan the available literature to create a cohesive review. Methodology: This review article is based on a literature search of PubMed, Google, and Google Scholar using the keywords “ Corona virus,” “COVID-19,” “COVID” “Origins,” “Review,” and “classification.” Articles providing new information were selected. Results: The coronavirus which has caused a pandemic has a genomic structure which closely resembles that of a bat coronavirus. The region that binds angi...

New AIV strain continue to emerge because of genetic instability of influenza virus and lack of p... more New AIV strain continue to emerge because of genetic instability of influenza virus and lack of proof reading and repair mechanism makes AIV genome easily prone to error that occur during replication. This study provide an update of H5N1 being an escape mutant which escaped from a immunized bird in aimmunization experiment conducted on birds with virus strain A/Duck/Tripura/103597/2008(H5N1). This article provide an update of H5N1 virus as it was evolving, creating the potential for new strain that is efficiently transmitted from birds to birds, birds to human or human to human remains highly lethal. The emergence of escape mutant may be either because of antigenic drift or genetic shift which results in H5N1 variants.

Indian Journal of Microbiology, 2020

Pesticide accumulation in agricultural soils is an environmental concern, often addressed through... more Pesticide accumulation in agricultural soils is an environmental concern, often addressed through distinct bioremediation strategies. This study has tried to analyze various soil bioremediation options viz., biostimulation, bioaugmentation, and natural attenuation in terms of efficiency and the response of autochthonous microbial flora by using atrazine as a model contaminant. Soil mesocosms were established with 100 kg of soil simulating the field conditions. The soil previously exposed to the herbicide was used for the bioaugmentation strategy undertaken in this study. We have tried to analyze how the microbial community responds to a foreign compound, both in terms of taxonomic and functional capacities? To answer this, we have analyzed metagenome of the mesocosms at a time point when 90% atrazine was degraded. Bioaugmentation for bioremediation proved to be efficient with a DT90 value of 15.48 ± 0.79 days, in comparison to the natural attenuation where the DT90 value was observed to be 41.20 ± 1.95 days. Metagenomic analysis revealed the abundance of orders Erysipelotrichales, Selemonadales, Clostridiales, and Thermoanaerobacterales exclusively in SBS mesocosm. Besides Pseudomonas, bacterial genera such as Achromobacter, Xanthomonas, Stenotrophomonas, and Cupriavidus have emerged as the dominant members in various bioremediation strategies tested in this study. Inclusive results suggest that inherent microbial flora adjust their community and metabolic machinery upon exposure to the pollutant. The site under pollutant stress showed efficient microbial communities to bio-remediate the newly polluted terrestrial ecologies in relatively less time and by economic means.

Current Drug Delivery, 2021

Objective: The objective of the current research work was to prepare chewable tablets having Acac... more Objective: The objective of the current research work was to prepare chewable tablets having Acacia catechu extract useful for mouth ulcers using a 32 factorial design. Methods: Acacia catechu heartwood extract was prepared using a reported method with some modifications. The extract was characterized using TLC against the catechin marker. Then, drug-excipient interaction studies were carried out. The mixture of drug and excipients was evaluated for pre-compression parameters. With the application of 32 factorial design, chewable tablets were prepared using direct compression technique. Prepared tablets were evaluated for post-compression parameters. Results: In vitrodrug release study of the developed formulations was investigated both in intact and crushed form of tablets. Based on in vitroperformance, the best formulations were selected (F6, F7 & F8 from intact and F1, F5 & F9 from the crushed group) and subjected to various kinetic models and evaluated for chewing difficulty ind...

Journal of the Practice of Cardiovascular Sciences, 2020

The world is in the midst of a corona virus disease (COVID-19) pandemic. We review a case of COVI... more The world is in the midst of a corona virus disease (COVID-19) pandemic. We review a case of COVID-19 myocarditis which was successfully managed and reported and also comment on the connection of COVID-19 and the heart.

Materials Today: Proceedings, 2020

Palladium nanoparticles (Pd NPs) with well-defined shape and controllable size were synthesized b... more Palladium nanoparticles (Pd NPs) with well-defined shape and controllable size were synthesized by Polyol strategy and the shape effect on hydrogen sensing at room temperature (RT) and beyond RT was detected. The evolution of shape in Pd NPs was dependent on the amount of stabilizer [Polyvinylpyrrolidone (PVP)] used during synthesis. Further, the dominant morphological facet in a particular shape determined whether the material will be suitable for room temperature or high temperature applications. The particle shape and facet hierarchy were analyzed using High Resolution Transmission Electron Microscopy (HRTEM) images, and Selected Area Electron Diffraction (SAED) patterns respectively. The hydrogen sensor studies revealed good response characteristics and the response parameters varied with the change in shape/facet characteristics of Pd nanoparticles. Also the variation of the hydrogen response with the increase in operating temperature was different for different shapes.

Indian Phytopathology, 2019

Apple stem grooving virus (ASGV) belonging to the genus Capillovirus under family Betaflexivirida... more Apple stem grooving virus (ASGV) belonging to the genus Capillovirus under family Betaflexiviridae is one of the widely distributed latent viruses mainly on pome fruit trees. It infects apple causing considerable economic losses that is a threat to the apple industry. To study the occurrence and incidence of ASGV disease, sensitive antisera based diagnostic tool has been developed, which would be helpful in large scale indexing, certification and quarantine programmes. Coat protein gene of ASGV was cloned into pET-32a(+) and pHIS-Parallel expression vectors. Expression characteristics of the proteins expressed from both the systems were compared and taken for raising the antisera. Sensitivity and specificity of the antiserum against virus infection was compared by Double Antibody Sandwich (DAS-ELISA). It was observed that the antisera raised from the protein expressed from pHIS-Parallel expression system (smaller ~ 3 kDa His tag) was more sensitive as compared to the antisera raised from the protein expressed from pET-32a(+) expression system (~ 18 kDa His tag) and the commercially available antisera. The antisera could also be successfully used for western blotting of infected samples and in DAS-ELISA. Thus, this study presents the first report on production of polyclonal antiserum against recombinant coat protein of ASGV from India, for reliable detection of the virus.

International Journal of Molecular Sciences

The early management, diagnosis, and treatment of emerging and re-emerging infections and the ris... more The early management, diagnosis, and treatment of emerging and re-emerging infections and the rising burden of non-communicable diseases (NCDs) are necessary. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas system has recently acquired popularity as a diagnostic tool due to its ability to target specific genes. It uses Cas enzymes and a guide RNA (gRNA) to cleave target DNA or RNA. The discovery of collateral cleavage in CRISPR-Cas effectors such as Cas12a and Cas13a was intensively repurposed for the development of instrument-free, sensitive, precise and rapid point-of-care diagnostics. CRISPR/Cas demonstrated proficiency in detecting non-nucleic acid targets including protein, analyte, and hormones other than nucleic acid. CRISPR/Cas effectors can provide multiple detections simultaneously. The present review highlights the technical challenges of integrating CRISPR/Cas technology into the onsite assessment of clinical and other specimens, along with cur...

Indian Journal of Chemical Technology

Advance inorganic fly ashmetakaolin-phosphatic geopolymer has been developed via greener approach... more Advance inorganic fly ashmetakaolin-phosphatic geopolymer has been developed via greener approach and deposited on mild steel substrate using spin-coating method, to improve the substrate-matrix interaction. The present research investigates the effect of incorporation of metakaolin and phosphate to geopolymer matrix to assess the performance of coating and optimization of spin coating parameters are performed as well to achieve maximum adhesion strength. XRD, FTIR, and SEM studies coupled with energy dispersive spectroscopy (EDX) have been carried out for determination of mineralogical phases, bonding interactions, substrate-matrix interface, and microstructural and chemical analysis.Results indicated that spin coating method produced well-ordered thin geopolymeric coating (thickness 13-20 micrometres), therefore spin coating deposition is the simplest way to obtain thin coatings of visibly thick materials. Maximum adhesion strength of material with substrate is 2.5M.Pa. which is obtained at optimized water to precursor ratio 0.5 at 500rpm spinning speed. It is concluded that new inorganic phases (originated from mutual contribution of phosphate, metakaolin and fly ash) like sodium iron aluminium phosphate, aluminium phosphate and sodium iron phosphate were responsible for good adhesion of material to the substrate and then related properties. The developed and optimized method can be used to produce homogeneous coating of heterogeneous geopolymer material on low carbon steel to indorse its advanced applications.

Rasayan Journal of Chemistry

The present work focuses on the growth of ZnO films vertically on indium tin oxide substrate usin... more The present work focuses on the growth of ZnO films vertically on indium tin oxide substrate using a wet chemical method. The morphological and structural study of the prepared films confirmed the formation of one-dimensional growth with a polycrystalline single-phase hexagonal wurtzite structure. The computed texture coefficient indicates the c-axis growth of the film. The optical spectra study exhibited a strong and sharp characteristics absorption band. The numerical derivative method and the Tauc plot method for the different transition values have been used to compare energy bandgap calculations.

Plant Disease Research, 2018

IJCT Vol.25(5) [September 2018], Sep 1, 2018

468-474In the present studies, three different compositions of geopolymeric coating material have... more 468-474In the present studies, three different compositions of geopolymeric coating material have been developed utilizing fly ash, red mud, alkali activators and sodium dihydrogen phosphate. TiO2 and Fe2O3 nanoparticles are added to the developed coating material compositions as additives. These compositions are applied to mild steel plates by spray coating technique. Further, adhesive strength, water stability and anti-corrosion behavior of geopolymeric coated mild steel plates are determined. Developed geopolymeric coating material compositions are characterized for minerology, bonding behavior and morphology by XRD, FTIR and SEM respectively. Results revealed that among all the three coating material compositions, composition containing nano TiO2 exhibited best performance in terms of adhesive strength [3.1 MPa at 7th day], water resistance and corrosion resistance characteristics in 3.5 weight % sodium chloride solution by electrochemical measurements

Clustered, Regularly Interspaced Short Palindromic Repeats and their associated Cas pro-teins (CR... more Clustered, Regularly Interspaced Short Palindromic Repeats and their associated Cas pro-teins (CRISPR-Cas) provide prokaryotes with a mechanism for defense against mobile genetic elements (MGEs). A CRISPR locus is a molecular memory of MGE encounters. It contains an array of short sequences, called spacers, that generally have sequence identity to MGEs. Three different CRISPR loci have been identified among strains of the opportunis-tic pathogen Enterococcus faecalis. CRISPR1 and CRISPR3 are associated with the cas genes necessary for blocking MGEs, but these loci are present in only a subset of E. faecalis strains. The orphan CRISPR2 lacks cas genes and is ubiquitous in E. faecalis, although its spacer content varies from strain to strain. Because CRISPR2 is a variable locus occurring in all E. faecalis, comparative analysis of CRISPR2 sequences may provide information about the clonality of E. faecalis strains. We examined CRISPR2 sequences from 228 E. faecalis genomes in relation...

B-cell exihibit B cell receptor or BCR, a transmembrane protein located on its surface. BCR compr... more B-cell exihibit B cell receptor or BCR, a transmembrane protein located on its surface. BCR comprises of two transmembrane proteins Igα and Igβ. Igβ (CD79B) is encoded by B29 gene. Leukaemic B-cells express low toundetectable Igs, Igβ (CD79B) proteins. Altered and aberrated B29 gene affect expression of Igβ/CD79B protein. One of the main factor of B29 gene alteration is mutation in their coding sequence. We analyzed the quantitative expression of B29 gene in leukaemic samples. Primers for B cell expression was designed using PrimerExpress 3.0 software. Designed primers were used to analyze the expression of B29 gene in different samples through ABI prism 7000 SDS system. Relative quantification of B29 gene in samples was done by 2^-ΔΔCt method. There was under expression of B29 gene in 100% of leukaemic samples. Though reason for lower gene expression in leukaemic samples was not detected yet there are number of papers reporting mutations and methylation as main cause of B29 gen alt...

Epidemiology and Infection, 2021

We report a familial cluster of 24 individuals infected with severe acute respiratory syndrome-co... more We report a familial cluster of 24 individuals infected with severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). The index case had a travel history and spent 24 days in the house before being tested and was asymptomatic. Physical overcrowding in the house provided a favourable environment for intra-cluster infection transmission. Restriction of movement of family members due to countrywide lockdown limited the spread in community. Among the infected, only four individuals developed symptoms. The complete genome sequences of SARS-CoV-2 was retrieved using next-generation sequencing from eight clinical samples which demonstrated a 99.99% similarity with reference to Wuhan strain and the phylogenetic analysis demonstrated a distinct cluster, lying in the B.6.6 pangolin lineage.

Environmental Quality Management, 2021

international journal of engineering trends and technology, 2014

B-cell exihibit B cell receptor or BCR, a transmembrane protein located on its surface. BCR compr... more B-cell exihibit B cell receptor or BCR, a transmembrane protein located on its surface. BCR comprises of two transmembrane proteins Igα and Igβ. Igβ (CD79B) is encoded by B29 gene. Leukaemic Bcells express low toundetectable Igs, Igβ (CD79B) proteins. Altered and aberrated B29 gene affect expression of Igβ/CD79B protein. One of the main factor of B29 gene alteration is mutation in their coding sequence. We analyzed the quantitative expression of B29 gene in leukaemic samples. Primers for B cell expression was designed using PrimerExpress 3.0 software. Designed primers were used to analyze the expression of B29 gene in different samples through ABI prism 7000 SDS system. Relative quantification of B29 gene in samples was done by 2^-ΔΔCt method. There was under expression of B29 gene in 100% of leukaemic samples. Though reason for lower gene expression in leukaemic samples was not detected yet there are number of papers reporting mutations and methylation as main cause of B29 gen alte...

Journal of the Practice of Cardiovascular Sciences, 2020

Objective: The coronavirus disease 2019 (COVID-19) also known as COVID-19 or severe acute respira... more Objective: The coronavirus disease 2019 (COVID-19) also known as COVID-19 or severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a viral infection caused by severe acute respiratory syndrome coronavirus 2, emerged first in Wuhan, China, and spread all over the world. The World Health Organization on January 30, 2020, officially declared the COVID-19 epidemic as a public health emergency of international concern. There is speculation as to the origin of the infection and the objective of this narrative review is to scan the available literature to create a cohesive review. Methodology: This review article is based on a literature search of PubMed, Google, and Google Scholar using the keywords “ Corona virus,” “COVID-19,” “COVID” “Origins,” “Review,” and “classification.” Articles providing new information were selected. Results: The coronavirus which has caused a pandemic has a genomic structure which closely resembles that of a bat coronavirus. The region that binds angi...

New AIV strain continue to emerge because of genetic instability of influenza virus and lack of p... more New AIV strain continue to emerge because of genetic instability of influenza virus and lack of proof reading and repair mechanism makes AIV genome easily prone to error that occur during replication. This study provide an update of H5N1 being an escape mutant which escaped from a immunized bird in aimmunization experiment conducted on birds with virus strain A/Duck/Tripura/103597/2008(H5N1). This article provide an update of H5N1 virus as it was evolving, creating the potential for new strain that is efficiently transmitted from birds to birds, birds to human or human to human remains highly lethal. The emergence of escape mutant may be either because of antigenic drift or genetic shift which results in H5N1 variants.

Indian Journal of Microbiology, 2020

Pesticide accumulation in agricultural soils is an environmental concern, often addressed through... more Pesticide accumulation in agricultural soils is an environmental concern, often addressed through distinct bioremediation strategies. This study has tried to analyze various soil bioremediation options viz., biostimulation, bioaugmentation, and natural attenuation in terms of efficiency and the response of autochthonous microbial flora by using atrazine as a model contaminant. Soil mesocosms were established with 100 kg of soil simulating the field conditions. The soil previously exposed to the herbicide was used for the bioaugmentation strategy undertaken in this study. We have tried to analyze how the microbial community responds to a foreign compound, both in terms of taxonomic and functional capacities? To answer this, we have analyzed metagenome of the mesocosms at a time point when 90% atrazine was degraded. Bioaugmentation for bioremediation proved to be efficient with a DT90 value of 15.48 ± 0.79 days, in comparison to the natural attenuation where the DT90 value was observed to be 41.20 ± 1.95 days. Metagenomic analysis revealed the abundance of orders Erysipelotrichales, Selemonadales, Clostridiales, and Thermoanaerobacterales exclusively in SBS mesocosm. Besides Pseudomonas, bacterial genera such as Achromobacter, Xanthomonas, Stenotrophomonas, and Cupriavidus have emerged as the dominant members in various bioremediation strategies tested in this study. Inclusive results suggest that inherent microbial flora adjust their community and metabolic machinery upon exposure to the pollutant. The site under pollutant stress showed efficient microbial communities to bio-remediate the newly polluted terrestrial ecologies in relatively less time and by economic means.

Current Drug Delivery, 2021

Objective: The objective of the current research work was to prepare chewable tablets having Acac... more Objective: The objective of the current research work was to prepare chewable tablets having Acacia catechu extract useful for mouth ulcers using a 32 factorial design. Methods: Acacia catechu heartwood extract was prepared using a reported method with some modifications. The extract was characterized using TLC against the catechin marker. Then, drug-excipient interaction studies were carried out. The mixture of drug and excipients was evaluated for pre-compression parameters. With the application of 32 factorial design, chewable tablets were prepared using direct compression technique. Prepared tablets were evaluated for post-compression parameters. Results: In vitrodrug release study of the developed formulations was investigated both in intact and crushed form of tablets. Based on in vitroperformance, the best formulations were selected (F6, F7 & F8 from intact and F1, F5 & F9 from the crushed group) and subjected to various kinetic models and evaluated for chewing difficulty ind...

Journal of the Practice of Cardiovascular Sciences, 2020

The world is in the midst of a corona virus disease (COVID-19) pandemic. We review a case of COVI... more The world is in the midst of a corona virus disease (COVID-19) pandemic. We review a case of COVID-19 myocarditis which was successfully managed and reported and also comment on the connection of COVID-19 and the heart.

Materials Today: Proceedings, 2020

Palladium nanoparticles (Pd NPs) with well-defined shape and controllable size were synthesized b... more Palladium nanoparticles (Pd NPs) with well-defined shape and controllable size were synthesized by Polyol strategy and the shape effect on hydrogen sensing at room temperature (RT) and beyond RT was detected. The evolution of shape in Pd NPs was dependent on the amount of stabilizer [Polyvinylpyrrolidone (PVP)] used during synthesis. Further, the dominant morphological facet in a particular shape determined whether the material will be suitable for room temperature or high temperature applications. The particle shape and facet hierarchy were analyzed using High Resolution Transmission Electron Microscopy (HRTEM) images, and Selected Area Electron Diffraction (SAED) patterns respectively. The hydrogen sensor studies revealed good response characteristics and the response parameters varied with the change in shape/facet characteristics of Pd nanoparticles. Also the variation of the hydrogen response with the increase in operating temperature was different for different shapes.

Indian Phytopathology, 2019

Apple stem grooving virus (ASGV) belonging to the genus Capillovirus under family Betaflexivirida... more Apple stem grooving virus (ASGV) belonging to the genus Capillovirus under family Betaflexiviridae is one of the widely distributed latent viruses mainly on pome fruit trees. It infects apple causing considerable economic losses that is a threat to the apple industry. To study the occurrence and incidence of ASGV disease, sensitive antisera based diagnostic tool has been developed, which would be helpful in large scale indexing, certification and quarantine programmes. Coat protein gene of ASGV was cloned into pET-32a(+) and pHIS-Parallel expression vectors. Expression characteristics of the proteins expressed from both the systems were compared and taken for raising the antisera. Sensitivity and specificity of the antiserum against virus infection was compared by Double Antibody Sandwich (DAS-ELISA). It was observed that the antisera raised from the protein expressed from pHIS-Parallel expression system (smaller ~ 3 kDa His tag) was more sensitive as compared to the antisera raised from the protein expressed from pET-32a(+) expression system (~ 18 kDa His tag) and the commercially available antisera. The antisera could also be successfully used for western blotting of infected samples and in DAS-ELISA. Thus, this study presents the first report on production of polyclonal antiserum against recombinant coat protein of ASGV from India, for reliable detection of the virus.