Prabal Ghosh - Academia.edu (original) (raw)

Papers by Prabal Ghosh

The Indian Journal of Animal Sciences

Blood samples were collected from six Ghungroo sows on the day of farrowing, day 7, day 15, day 3... more Blood samples were collected from six Ghungroo sows on the day of farrowing, day 7, day 15, day 30, day 45 and day 60 post farrowing to investigate the alterations in the physio-biochemical, endocrine and in vitro activity of immune effector cells in Ghungroo sow during different days of lactation cycle. The hematological parameters were evaluated by standard hematological procedure. Blood biochemical and endocrine profiles were evaluated by commercially available kits. In vitro phagocytic activity and lymphocyte proliferation response were evaluated by colorimetric NBT and MTT assay respectively. Haemoglobin, packed cell volume (PCV) and total erythrocyte counts (TEC) increased significantly during the early lactation periods from the day of farrowing. The lymphocyte numbers decreased significantly from the day of farrowing to the 1st week of lactation cycle. Blood glucose and cholesterol level decreased significantly from the day of farrowing till first week of lactation cycle. Th...

Veterinary World

The RFamide-related peptides (RFRPs) are the group of neuropeptides synthesized predominantly fro... more The RFamide-related peptides (RFRPs) are the group of neuropeptides synthesized predominantly from the hypothalamus that negatively affects the hypothalamo-hypophyseal-gonadal (hypothalamic–pituitary–gonadal [HPG]) axis. These peptides are first identified in quail brains and emerged as the mammalian orthologs of avian gonadotropin inhibitory hormones. The RFRP-3 neurons in the hypothalamus are present in several mammalian species. The action of RFRP-3 is mediated through a G-protein-coupled receptor called OT7T022. The predominant role of RFRP-3 is the inhibition of HPG axis with several other effects such as the regulation of metabolic activity, stress regulation, controlling of non-sexual motivated behavior, and sexual photoperiodicity in concert with other neuropeptides such as kisspeptin, neuropeptide-Y (NPY), pro-opiomelanocortin, orexin, and melanin. RFamide peptides synthesized in the granulosa cells, interstitial cells, and seminiferous tubule regulate steroidogenesis and g...

The Journal of Agricultural Education and Extension, 2021

This paper explored the impact of prolonged lockdown caused by coronavirus disease (COVID)-19 pan... more This paper explored the impact of prolonged lockdown caused by coronavirus disease (COVID)-19 pandemic on the academic aspects of veterinary students of India and its relationship with their socio-...

Biological Rhythm Research, 2019

Blood samples (5 mL/animal) were collected from male (n = 50) and female (n = 50) black Bengal go... more Blood samples (5 mL/animal) were collected from male (n = 50) and female (n = 50) black Bengal goat kids to evaluate the alterations in plasma Na, K, Ca and Fe during pre-pubertal growth phase. The plasma sodium concentration significantly (P < 0.01) decreased with the advancement of age in both male and female kids. Plasma K did not differ significantly between age group and sex. There was a significant (P < 0.01) increase in plasma calcium level. There was no significant alteration in iron levels during different days of pre-pubertal period in the kids of both sexes. Baseline data of plasma Na, K, Ca and Fe levels in black Bengal kids of either sexes during different days of pre-pubertal growth stage generated in this study will be required to calculate mineral requirements in goats during growth phase. It can be recommended that during ration formulation for goat kids in early growth stage, mineral levels do not need to be adjusted based on sex, as sex had no influence over...

Materials Today Communications, 2021

Abstract Musculoskeletal disorders and bone deficiencies have been established among the most imp... more Abstract Musculoskeletal disorders and bone deficiencies have been established among the most important human health conditions that exist today. Because of the numerous and complex functions of bone, there are many disorders, such as osteoarthritis, rheumatoid arthritis, osteoporosis and bone cancer that arise with time. Conventional therapy alone does not always yield satisfactory results and the treatment protocol is difficult, time consuming and expensive. Systemic therapy has the disadvantages of the need for long-term treatment due to being unable to create a high local concentration and the resultant risk of toxicity in addition to problems of drug transport and cost. As a result, intensive research activity on targeted local delivery of drugs/growth factors has been initiated. Local drug delivery is potentially an advantage over standard oral/intravenous therapy/other modalities. In the present review, we have highlighted various drugs/growth factors/molecules and their potential local delivery systems for effective management of bone disorders.

International Journal of Livestock Research, 2020

Veterinarski arhiv, 2020

Expression profile of growth hormone receptor (GHR), insulin like growth factor receptor (IGFR) a... more Expression profile of growth hormone receptor (GHR), insulin like growth factor receptor (IGFR) and leptin genes, with associated blood bio-chemicals and endocrines profiles in black Bengal kids (Capra hircus) during different stages of pre-pubertal growth. vet. arhiv 90, 243-254, 2020. aBSTRaCT The aim of this study was to evaluate the expression profile of growth hormone receptor (GHR), insulin like growth factor receptor (IGFR) and leptin genes, together with some blood biochemical (glucose, aspartate aminotransferase, AST; alanine aminotransferase, ALT) and endocrine (growth hormone and cortisol) parameters. For this purpose, blood samples were collected from 25 male and 25 female kids of the Black Bengal goat (Capra hircus), at days 15, 30, 60, 90, 120 and 150. Male kids gained more body weight compared to females. The glucose and cortisol levels significantly (P≤0.01) decreased with the advancement of age. AST, ALT, and growth hormone increased significantly (P≤0.01). In both sexes, plasma glucose level was negatively (P≤0.01) correlated with AST. In males both cortisol and GH levels were positively (P≤0.05) correlated with plasma glucose levels. In female kids only GH showed a positive (P≤0.05) correlation with glucose levels. Female kids had greater expression of the GH receptor, IGFR mRNA and Leptin mRNA on day 30 (P≤0.05) and day 150 (P≤0.01). GHR was only positively (P≤0.05) correlated with IGFR and LEP in female kids. On the basis of the results obtained from our research, we are able to conclude that the lower growth rate in female kids expressed more leptin, which stimulated the somatotrophic axis for the expression of GHR and IGFR during the prepubertal growth stage.

Biological Rhythm Research, 2018

The study was carried out to investigate hemato-biochemical profile and blood metabolites during ... more The study was carried out to investigate hemato-biochemical profile and blood metabolites during different periods of pre-pubertal growth in summer-and winter-born black Bengal kids of either sexes. The body weight was 8.07 ± 0.21 kg on 180 day with significantly (P < 0.01) higher growth in male kids. The winter born kids exhibited significantly (P < 0.01) higher body weight, packed cell volume (PCV), hemoglobin (Hb), total leukocyte counts (TLC), glucose and cholesterol and significantly (P < 0.01) lower non-esterified fatty acids (NEFA) and α-amino nitrogen (AAN). Both PCV and Hb were lowest on day 15 and then increased significantly (P ≤ 0.01) and reached maximum value at 90 days (PCV) and 180 days (Hb). TEC increased significantly (P ≤ 0.01) from day 15 to 180 days and reached its maximum value at 180 days age. Both glucose and total cholesterol were maximum at day 15 and significantly (P ≤ 0.01) decreased with the advancement of age and found lowest was at day 150. But, total protein was lowest at day 15 and increased significantly (P ≤ 0.01) up to day 150. NEFA and AAN were lowest on day 15 and increased significantly (P ≤ 0.01) with the advancement of age and reached peak value on 180 days.

Veterinary world, 2017

The present investigation was conducted to study the association between udder morphology and in ... more The present investigation was conducted to study the association between udder morphology and in vitro activity of milk leukocytes in high yielding crossbred cows. A total of 48 healthy high yielding crossbred cows were selected for the study. The udder configuration and teat/udder morphology were recorded before milking. Milk samples (100 ml/cow) were collected aseptically. Milk somatic cell counts (SCC) and milk differential leukocyte counts were performed microscopically. Milk leukocytes (viz., neutrophils, lymphocytes, and macrophages) were isolated from milk samples by density gradient centrifugation. Phagocytic index (PI) of milk neutrophils and macrophages were evaluated by colorimetric nitro blue tetrazolium assay. Lymphocytes proliferation response was estimated by MTT assay and expressed as stimulation index. There was a significant (p<0.01) positive correlation between milk SCC with mid teat diameter, teat base diameter and significant (p<0.05) negative correlation ...

Biological Rhythm Research, 2016

Abstract The role of lactogenic hormones (prolactin, growth hormone, cortisol and thyroid hormone... more Abstract The role of lactogenic hormones (prolactin, growth hormone, cortisol and thyroid hormone) on lactation yield in Mithun cows as well as their rhythmicity throughout the lactation cycle were studied in Mizoram (n = 4) and Nagaland (n = 7) strain of mithun (Bos frontalis). Blood samples were collected from all the animals from the day of calving to the complete dry off at an interval of 15 days. All the hormones were estimated in the serum by commercially available ELISA kits. Plasma level of cortisol (μg/dl), growth hormone (GH, in ng/ml), prolactin (PRL, in μIU/ml), triiodothyronine (T3, in nmol/μl) and thyroxin (T4, in ng/ml) were 20.84 ± 0.29, 28.08 ± 0.56, 9.87 ± 0.20, 27.82 ± 0.56 and 51.33 ± 0.48, respectively, in mithun irrespective of strains during the lactation period. Levels of all the hormones varied significantly (p ≤ 0.01) during different days of lactation cycle but, there was no significant difference among strain. Levels of PRL, GH, cortisol and T3 were significantly (p < 0.01) higher around calving and declined sharply. The hormones remained in almost steady state during mid-lactation and declined during late lactation. All the hormones stated above were positively correlated with lactational yield thus their role on lactogenesis and galactopoiesis was established.

Veterinary World, 2015

The present study has been conducted to evaluate the variation in electrocardiogram (ECG) paramet... more The present study has been conducted to evaluate the variation in electrocardiogram (ECG) parameters among different trained breeds of dogs (viz. Labrador, German Shepherd, and Golden Retriever) used for security reasons. Materials and Methods: The ECG was recorded by single channel ECG at a paper speed of 25 mm/s and calibration of 10 mm=1 mV. The recordings were taken from all the standard bipolar limb leads (Lead-I, II, and III) and unipolar augmented limb leads (Lead-aVR, aVL, and aVF). Results: Heart rate was found to be highest in Labrador and lowest in German Shepherd. P-wave duration was maximum in Golden Retriever breed and lowest in Labrador. Maximum amplitude of P-wave was found in Labrador followed by German Shepherd and Golden Retriever. There was significantly (p<0.05) higher values of PR interval in German Shepherd compared to other breeds. The variation in QRS duration, ST segment duration, T-wave duration, and T-wave amplitude was found to be non-significant among breeds. Inverted T-waves were most common in Golden Retriever and German Shepherd, whereas positive T-waves were found in Labrador. There was significant (p<0.05) variation in mean electrical axis of QRS complex among different breeds and it ranges from +60° to +80°. Conclusion: The present study provides the reference values for different ECG parameters to monitor the cardiac health status among Labrador, German Shepherd, and Golden Retriever breeds.

October-2020

Aim: The present investigation was intended to generate some basic data on electrocardiography (E... more Aim: The present investigation was intended to generate some basic data on electrocardiography (ECG) parameters in different breeds and their alterations in respect to sex and age. Materials and Methods: The present investigation was carried out on 239 owned dogs of 11 different breeds presented to the Institute Veterinary Clinic during 2018-2019. The animals, irrespective of breed and sex were grouped on the basis of their age groups. Recordings of ECG were performed by a single-channel ECG machine (CardiartR, BPL, India) at 25 mm/s paper speed and 10 mm=1 mV calibration keeping the animals on the right lateral recumbence without any anesthesia. Heart rate, along with the amplitude and duration of different waves and complexes was measured. Results: Heart rate did not vary significantly among breeds, sex, and different age groups. The highest heart rate has been reported in Doberman and the lowest in Beagle. The heart rate was lowest at the age group of 6 months-2.5 years and highe...

The DNA barcoding of Felidae (cat family, using mitochondrial Cytochrome oxidase subunit-I (COI) ... more The DNA barcoding of Felidae (cat family, using mitochondrial Cytochrome oxidase subunit-I (COI) region opens a new vista for molecular identification of different life forms on our planet and caters a boon for cataloguing of taxa. In the present study, an attempt is made to develop a set of universal primer (forward COI-Fl and reverse COI-R1) for PCR amplification of COI gene for higher mammals and validate by successful amplification and subsequent sequencing of the gene in Royal Bengal Tiger (Panthera tigris) and domestic cat (Felts sylvestris catus). The sequenced data are submitted in GenBank (Accession No FJ185309 and FJ185310) as DNA barcode of the species. The designed primers opened a new hope in mitigating a long standing problem of species identification due to poaching of wild animals, adulteration of meat and animal products and purity testing as it can amplify wide range of barcode sequences of higher mammals. The obtained sequences along with other relevant sequences ...

Poultry biodiversity widely prevails over the world, especially in Indian subcontinent as domesti... more Poultry biodiversity widely prevails over the world, especially in Indian subcontinent as domestic indigenous fowl. Cross species hybridization of different Gallus species confers ambiguity in proper classification. Molecular approach using DNA barcoding to identify species boundaries is successfully employed to detect hybridization signal of nondescript indigenous fowls. In the present study DNA barcoding region of cytochrome oxidase I (COI) gene collected from geographically distant region of five agroclimatic zones of eastern India is amplified using PCR, sequenced and subsequently deposited in GenBank. Related sequences were retrieved from global databases for phylogenic analysis using bioinformatics. The present study provides for the first time the DNA barcodes of domestic fowls from eastern India and established that the domestic fowls are grouped within the group Gallus gallus. Additional COI sequences within the same and related genus collected from different countries prop...