Renata Falchete do Prado - Academia.edu (original) (raw)

Papers by Renata Falchete do Prado

Brazilian dental science, 2023

Objective: to evaluate the differentiation and gene expression of transcripts related to osteogen... more Objective: to evaluate the differentiation and gene expression of transcripts related to osteogenesis in a primary culture of Mesenchymal Stem Cells (MSCs) derived from rat femurs submitted to radiotherapy and the installation of pure titanium implants. Material and Methods: fifty-four rats received titanium implants in both femurs and were divided into three groups: Control: implant surgery (C); Implant + immediate irradiation (IrI), and Implant + late irradiation (IrL). Euthanasia occurred 3, 14, and 49 days after surgery. The bone marrow MSCs from the femurs were isolated and cultivated. The cell viability, total protein content, alkaline phosphatase (ALP) activity, and the formation of mineralization nodules and cellular genotoxicity were analyzed. The gene expression of Alkaline Phosphatase (phoA), Collagen 1 (COL1), Runt-related transcription factor 2 (RUNX2), Osterix (OSX), Osteopontin (OPN), Integrin β 1 (ITGB 1), Bone Sialoprotein (BSP), Osteonectin (SPARC), Osteocalcin (Bglap), Transforming Growth Factor β-type (TGF-β), Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF), Interleukin-6 (IL-6), Apolipoprotein E (APOE) and Prostaglandin E 2 synthase (PGE 2) were evaluated by qRT-PCR. Results: ionizing radiation suppresses the gene expression of essential transcripts for bone regeneration, as well as cellular viability, as observed in the IrI and IrL groups. Conclusion: although this can lead to the loss of osseointegration and failure of the implant, the MSCs showed more activity at 49 days than at 3 and 14 days.

Brazilian Oral Research, 2004

Medicina Oral Patologia Oral Y Cirugia Bucal, Jun 1, 2006

Brazilian Dental Science, 2012

Preventive measures in Dentistry and the modernization of the orthodontics appliances led adult p... more Preventive measures in Dentistry and the modernization of the orthodontics appliances led adult patients to seek orthodontic treatment to improve their appearance and social acceptance, as well as function and oral health. Adult patients present some peculiarities such as periodontal disease and tooth loss which complicates the mechanotherapy, requiring a multidisciplinary team and a more complex mechanics. Thus, the aim of this study was to verify the proportion of young and adult patients who started orthodontic treatment in the period from 2000 to 2010, as well as the total percentage, and to correlate male and female values. The sample of 3,272 patients was divided into three groups according to the age: up to 20 (group 1), from 20 to 40 (group 2) and above 40 years-old (group 3), according to gender. It was found that most patients were young (group 1: 71.85%). However, the number of adults from 20 to 40 years-old represents a considerable share of 26.52%. Above 40 years-old, 1...

Medicina oral, patología oral y cirugía bucal, 2006

Pleomorphic adenomas are the most frequent type of epithelial salivary gland neoplasms, and their... more Pleomorphic adenomas are the most frequent type of epithelial salivary gland neoplasms, and their malignant counterpart, the carcinoma in pleomorphic adenomas, is much less common. Beta-catenin is a cell adhesion molecule associated with the invasion and metastasis of carcinomas of the head and neck, esophagus. The objective of this study was to detect the expression of beta-catenin in pleomorphic adenomas, carcinomas in pleomorphic adenomas and normal salivary glands to discuss its role in the development of these two lesions. The expression of beta-catenin (BD Transduction Laboratories) was analyzed by immunohistochemistry in formalin-fixed, paraffin embedded specimens by the avidin-biotin-peroxidase complex method in 16 pleomorphic adenomas (12 from minor salivary glands), 3 carcinomas in pleomorphic adenomas (all from palate) and 10 normal salivary glands as control group (5 from major and 5 from minor salivary glands). All cases of glands, adenomas and carcinomas in pleomorphic...

Journal of Applied Oral Science, 2014

The aim of this paper was to evaluate the repair of onlay autogenous bone and Methods: Eighty fem... more The aim of this paper was to evaluate the repair of onlay autogenous bone and Methods: Eighty female rats were randomly divided into two groups: ovariectomized (OVX) and with a simulation of the surgical procedure (SHAM). Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM). Animals were euthanized on days 0, 7, 21, 45, and 60. The specimens were subjected to immunohistochemistry for bone sialoprotein (BSP), osteonectin (ONC), and osteocalcin (OCC). Results: All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense. At the 45-day, the bone graft showed direct bonding to the recipient bed in all specimens. The ONC and OCC showed more expressed in granulation tissue, in

Rev Bras Patol Oral, Dec 1, 2003

Brazilian Dental Science, 2021

Objective: To evaluate the efficacy of different fluoride varnishes on white spot lesions (WSL) r... more Objective: To evaluate the efficacy of different fluoride varnishes on white spot lesions (WSL) remineralization. Material and Methods: Polished bovine enamel specimens were obtained (n = 60) and had their initial surface Knoop microhardness (SMH) determined. WSL were created and the SMH was measured again. Then, specimens were allocated into six groups: C – Control (without varnish); BF – Bifluorid 12 (6% NaF + 6% CaF2); DP – Duraphat (5% NaF); PF – Profluorid (5% NaF); FP - Fluor Protector (0.2% NaF + 0.9% difluorsilane); CW - Clinpro White Varnish (5% NaF + 5% TCP). After varnishes application, specimens were immersed in artificial saliva for 24 h. Then, pH-cycling was performed for 8 days and SMH was measured. Data were analyzed by one-way ANOVA and Tukey’s test. Results: Non-significant differences were observed among the groups at baseline (p = 0.187) and after WSL formation (p = 0.999). After treatments, significant differences were observed among the groups (p = 0.001). Mean...

Research, Society and Development, 2021

The aim of this study was to investigate the effect of previous exposure to CsA on the expression... more The aim of this study was to investigate the effect of previous exposure to CsA on the expression of RANK and bone loss in experimental periodontitis induced in rats. Forty rats were divided into four groups as follows: Control (CON); Cyclosporine A (CsA), with daily doses of 10 mg/Kg of CsA; Ligature (LIG), with a ligature around the upper right second molar on day 30; and Ligature + CsA (CsAL). Sixty days later, the animals were sacrificed and samples removed for histomorphometric and immunohistochemical analysis of bone resorption, bone density and RANK expression. There was no significant influence of bone resorption on the application of CsA. LIG Group (735.88 ± 121.82) had significantly higher bone resorption (p = 0.01) than CON Group (569.13 ± 89.76), and CsAL Group (759.38 ± 198.23) also had significantly higher bone resorption (P = 0.001) than CsA Group (410.90 ± 105.95). Bone density in the CsA Group (87.49 ± 4.07) was lower than that of CON Group (92.42 ± 2.27) and simila...

Journal of Functional Biomaterials, 2021

Background: Several studies proved that anodic oxidation improves osseointegration. This study ai... more Background: Several studies proved that anodic oxidation improves osseointegration. This study aimed to optimize osseointegration through anodization in dental implants, obtaining anatase phase and controlled nanotopography. Methods: The division of the groups with 60 titanium implants was: control (CG); sandblasted (SG); anodized (AG): anodized pulsed current (duty cycle 30%, 30 V, 0.2 A and 1000 Hz). Before surgery, surface characterization was performed using Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM), X-ray Dispersive Energy Spectroscopy (EDS) and Raman Spectroscopy. For in vivo tests, 10 New Zealand white rabbits received an implant from each group. The sacrifice period was 2 and 6 weeks (n = 5) and the specimens were subjected to computed microtomography (μCT) and reverse torque test. Results: AFM and SEM demonstrated a particular nanotopography on the surface in AG; the anatase phase was proved by Raman spectroscopy. In the μCT and in the reverse torque...

Journal of Materials Science: Materials in Medicine, 2020

Biomaterials may be useful in filling lost bone portions in order to restore balance and improve ... more Biomaterials may be useful in filling lost bone portions in order to restore balance and improve bone regeneration. The objective of this study was to produce polycaprolactone (PCL) membranes combined with two types of bioglass (Sol-Gel and melt-quenched) and determine their physical and biological properties. Membranes were produced through electrospinning. This study presented three experimental groups: pure PCL membranes, PCL-Melt-Bioglass and PCL-Sol-gel-Bioglass. Membranes were characterized using Scanning Electron Microscopy, Fourier Transform Infrared Spectrophotometry (FTIR), Energy-Dispersive Spectroscopy and Zeta Potential. The following in vitro tests were performed: MTT assay, alkaline phosphatase activity, total protein content and mineralization nodules. Twenty-four male rats were used to observe biological performance through radiographic, fracture energy, histological and histomorphometric analyses. The physical and chemical analysis results showed success in manufacturing bioactive membranes which significantly enhanced cell viability and osteoblast differentiation. The new formed bone from the in vivo experiment was similar to that observed in the control group. In conclusion, the electrospinning enabled preparing PCL membranes with bioglass incorporated into the structure and onto the surface of PCL fibers. The microstructure of the PCL membranes was influenced by the bioglass production method. Both bioglasses seem to be promising biomaterials to improve bone tissue regeneration when incorporated into PCL.

Clinical Oral Investigations, 2019

Objectives Evaluate the modulating effect of ionizing radiation, blood cytokine levels, and bone ... more Objectives Evaluate the modulating effect of ionizing radiation, blood cytokine levels, and bone remodeling of the interface around the implant to understand the radiation mechanisms which can impair the implants receptor site. Material and methods Sixty rats were submitted to grade V titanium implants in the femurs and were divided into the following groups: no-irradiation (N-Ir): control group with implant only; early-irradiation (E-Ir): implant + irradiation after 24 h; lateirradiation (L-Ir): implant + irradiation after 4 weeks; and previous-irradiation (P-Ir): irradiation + implant after 4 weeks. The animals in the E-Ir, L-Ir, and P-Ir groups were irradiated in two fractional stages of 15 Gy. At 3 days, 2 weeks, and 7 weeks after the final procedure, five animals were randomly euthanized per group. Serum levels of TNF-ɑ, IL-1β, TGF-β, IL-6, M-CSF, and IL-10 were measured from blood collected prior to euthanasia using the ELISA test. The pieces containing the implants were subjected to immunohistochemical labeling using the tartrate acid resistant to phosphatase, osteocalcin, and caspase-3 markers and mCT. The ANOVA test was used for statistical analysis, and the Tukey multiple comparison test (p < 0.05) was applied. Results The results indicated that ionizing radiation modifies the production of pro-and anti-inflammatory serum cytokines, the expression of proteins involved in bone remodeling and cellular apoptosis, as well as changes in bone formation. Conclusions The results suggests that a longer period between radiotherapy and implant placement surgery when irradiation occurs prior to implant installation would allow the recovery and renewal of bone cells and avoid future failures in osseointegration. Clinical relevance The search for modifications caused by ionizing irradiation in bone tissue can indicate the ideal period for implant placement without affecting the osseointegration process.

Journal of Oral Biology and Craniofacial Research, 2019

The most important microscopic characteristic of Cyclosporine A-induced gingival overgrowth is fi... more The most important microscopic characteristic of Cyclosporine A-induced gingival overgrowth is fibroepithelial hyperplasia. Objective: The objective was to investigate the influence of previous exposure to Cyclosporine A over gingival epithelium in experimental periodontitis in rats. Methods: Twenty Wistar rats with 12 weeks-old were divided into four groups with 5 animals each: Control Group (CG); Cyclosporine Group (CsAG); Ligature group (LG) and Cyclosporine and Ligature Group (CsALG). Daily doses of CsA (10 mg/kg) were applied to CsAG and CsALG during 60 days since the beginning of the experiment and, a ligature was placed in LG and CsALG 30 days after the beginning of the experiment. After 60 days, animals were euthanized and gingival tissue was processed to histomorphometric analysis of epithelial thickness (mm 2), immunohistochemical expression of PCNA (%) and inflammatory response. Data were analyzed by Kruskal-Wallis and Mann Whitney at 0.05 significance level. Results: Considering epithelial thickness, CG was thinner than all groups, CsALG was the largest and CsAG and LG were similar between each other. Regarding the PCNA expression CG (16.46 ± 9.26) was similar to CsAG (34.47 ± 19.75) and, LG (59.02 ± 10.33) was similar to CsALG (40.59 ± 18.25). Significant difference (p < 0.05) occurred only in inflammation presence comparing CG/LG and CsAG/CsALG. A weak positive correlation between the number of PCNA+ and inflammatory cells (p = 0.001; r = 0.611) was observed. Conclusion: Based on these results it was concluded that the enlargement of gingival epithelium observed in experimental periodontitis can be increased by previous exposition to CsA and inflammatory conditions enhanced proliferative activity of the keratinocytes.

Journal of oral biology and craniofacial research

The host's answer has an important role in periodontal disease, and the mast cells have a pri... more The host's answer has an important role in periodontal disease, and the mast cells have a prime role. Such cells seem to be influenced by estrogen deficiency. The objective was to evaluate the mast cells and the expression of metalloproteinase(MMP)-9 in periodontal disease induced in ovariectomized rats. For that purpose, 36 rats were used; 18 ovariectomized (OVX) and another 18 Sham-operated (SHAM). After 60 days the periodontal disease was induced by a ligature around the first lower right molars (group P). The opposite side was the control group (group C). The euthanasia occurred 3, 7 and 14 days after the placement of the ligature. The gingiva was removed and analyzed histochemically and immunohistochemically to quantify the mast cells and to analyze MMP 9 expression. By comparing the groups SHAM-P and C and groups OVX-C and P, it was noted that mast cells from group C were higher than P in all experimental periods. When comparing groups SHAM-C and OVX-C, significant factors...

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 2017

A 39-year-old man underwent surgical excision of a keratocystic odontogenic tumor in his left man... more A 39-year-old man underwent surgical excision of a keratocystic odontogenic tumor in his left mandibular branch via an intraoral route. After 2 years, during radiographic postoperative follow-up, osteolysis was observed on the left mandibular branch in the lingual region, suggesting tumor recurrence. Considering the difficulty of removing the residual lesion via an intraoral route and the anatomy of the medial region of the branch, we opted to perform a vertical mandibular branch osteotomy technique with extraoral access according to the Risdon technique. Vertical osteotomy provided direct access to the tumor by segmentation of the branch; the proximal stub was pulled laterally, the distal stub was pulled medially, and enucleation was performed with peripheral osteotomy for the treatment of a small recurrent keratocystic odontogenic tumor. The patient was followed-up for 18 months; there is no clinical evidence of recurrence to date.

International Endodontic Journal, 2017

Aim To establish an SV40 TAg -transfected cell line of human pulp-derived cells in order to compa... more Aim To establish an SV40 TAg -transfected cell line of human pulp-derived cells in order to compare the cytotoxicity, genotoxicity and to investigate the activities of immunological biomarkers of several endodontic sealers. Methodology Primary human pulp cells and transfected cells were cultured. Cell morphology and proliferation were analyzed, and the expression of cell-specific gene transcripts and proteins was detected by RT-PCR and immunohistochemistry. Transfection of human pulp-derived cells resulted in an immortalized cell line retaining phenotypic characteristics from the primarily cells tested. The SV40 TAg -transfected cells were cultured and stimulated by sealers (Apexit Plus, Real Seal, AH Plus, and EndoREZ) in order to evaluate the cytotoxicity and genotoxicity by MTT and MTN assays, respectively. Immunological inflammatory biomarkers (IL6, IL8 and TNF-α) were determined by ELISA assay. The differences between median values were statistically analyzed using Kruskal-Wallis and Dunn's tests at 5% significance level. Results The cytotoxicity assay revealed that multi-methacrylate (Real Seal) was the most cytotoxic sealer (p<0.05) and exhibited the highest inflammatory potential against the SV40 TAg -transfected cells (p<0.05). All root canal sealers tested were able to stimulate the immortalized pulp cells to produce IL-6,-8 and TNF-α, with differences in relation to the control group (p<0.05). Higher levels of IL-6,-8 and TNF-α were found in cell supernatant after stimulation with multi-methacrylate (Real Seal) compared to all other sealers tested (p<0.05). No differences were found comparing Epoxy resin-based sealer (AHPlus), single-methacrylate sealer (EndoREZ) and calcium hydroxide-based sealer (Apexit Plus), regardless of the cytokine investigated (all p>0.05). Conclusions A SV40 TAg -transfected cell line of human pulp-derived cells was established. The methacrylate resin-based sealer (RealSeal) exhibited the greatest cytoxicity and inflammatory potential against immortalized pulp cells than an epoxy resin-based sealer (AH Plus), a methacrylatebased sealer (EndoRez), and a calcium hydroxide-based sealer (Apexit).

Acta Stomatologica Croatica, 2016

Sažetak Torus palatinus jednostruka je ili višestruka egzostoza koja se pojavljuje u srednjoj lin... more Sažetak Torus palatinus jednostruka je ili višestruka egzostoza koja se pojavljuje u srednjoj liniji tvrdoga nepca. Smatra se uobičajenim kliničkim nalazom. Kako se magnetska rezonancija (MR) u dentalnoj medicini ne upotrebljava često, rijetki su opisi te koštane izrasline na temelju takve metode. U ovom istraživanju opisan je slučaj pacijentice koja je upućena doktoru dentalne medicine da ispita koštano zadebljanje otkriveno magnetskom rezonancijom učinjenom radi dijagnostike migrene. Obavljeni su i dodatni dentalno-medicinski pregledi radi planiranja različitih terapijskih zahvata pa oni, uz MR, upotpunjuju opis torusa palatinusa sa svrhom dobivanja cjelovite slike kliničkog nalaza.

Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 2016

Objective: Despite the advances in medicine, the oral squamous cell carcinomas still have high in... more Objective: Despite the advances in medicine, the oral squamous cell carcinomas still have high incidence and are very important to study topics such as their lymphatic and microvessel density. This research assessed the correlation between histological grading, formation of new lymphatic and blood vessels and cell proliferation in squamous cell carcinomas (SCC). Methods: Twenty-nine oral floor SCC and 30 tongue cases were assessed for their clinical characteristics and histological grading of malignancy. Results: The presence of VEGF (vascular endothelial growth factor VG1), VEGF-C (vascular endothelial growth factor-C), CD105, to determine blood microvascular (MVD), and D2-40 to determine lymphatic density (LMD) was investigated using immunohistochemistry. Histological grading revealed that 73.3% of tongue and 96.67% of floor cases were classified as highly aggressive. Cell proliferation was greater on the floor; however, no significant difference was observed. Most carcinomas were VEGF negative (tongue 63.3% and floor 70.0%) and VEGF-C positive (tongue 73.3% and floor 79.3%). LMD was considerably greater on the tongue. High MVD values occurred in cases with greater cell proliferation. No relationship was determined between the growth factors VEGF and VEGF-C and MVD and LMD, respectively. Conclusion: Floor of mouth SCC cases was morphologically more aggressive than tongue cases; however, in tongue carcinomas a greater quantity of lymphatic vessels could represent potential ways for locoregional cell dissemination of the neoplastic cells, independent of histological grading. Blood vascularization presented correlation with cell proliferation in intraoral squamous cell carcinoma and could be useful in the prognostic assessment of this neoplasm.

Medical Molecular Morphology, 2016

Lymphatic dissemination is one of the most important pathways for metastasis in many solid tumors... more Lymphatic dissemination is one of the most important pathways for metastasis in many solid tumors, including head and neck carcinomas. The lymphatic growth of cancer has been used as a significant independent adverse prognostic factor and provides information about tumor progression. Salivary gland tumors present different prognoses and have the ability to develop metastases; however, this information regarding the lymphatic spread is scarce. This paper quantifies the lymphatic microvessel density (LMD) in benign and malignant salivary gland tumors and analyzes the relationship between LMD and tumor expression of vascular endothelial growth factors C (VEGF-C) and the proliferative index. The results show that there is no correlation between LMD, VEGF-C and the proliferative index in the majority of salivary gland tumors analyzed, apart from polymorphous low-grade carcinoma which exhibits statistical correlation between LMD and the proliferative index (p \ 0.05). This correlation probably does not indicate a poor prognosis for this PLGA, since this is a low metastasizing carcinoma of the salivary glands. Different from other solid tumors, such as breast or prostatic carcinomas, there is no correlation between VEGF-C and LMD in salivary gland tumors, and so these traits are not able to estimate the metastatic risk or the prognosis of these tumors.

Journal of Materials Science: Materials in Medicine, 2015

Tests on titanium alloys that possess low elastic modulus, corrosion resistance and minimal poten... more Tests on titanium alloys that possess low elastic modulus, corrosion resistance and minimal potential toxicity are ongoing. This study aimed to evaluate the behavior of human osteoblastic cells cultured on dense and porous Titanium (Ti) samples comparing to dense and porous Ti-35 Niobium (Ti-35Nb) samples, using gene expression analysis. Scanning electronic microscopy confirmed surface porosity and pore interconnectivity and X-ray diffraction showed titanium beta-phase stabilization in Ti-35Nb alloy. There were no differences in expression of transforming growth factor-b, integrin-b1, alkaline phosphatase, osteopontin, macrophage colony stimulating factor, prostaglandin E synthase, and apolipoprotein E regarding the type of alloy, porosity and experimental period. The experimental period was a significant factor for the markers: bone sialoprotein II and interleukin 6, with expression increasing over time. Porosity diminished Runtrelated transcription factor-2 (Runx-2) expression. Cells adhering to the Ti-35Nb alloy showed statistically similar expression to those adhering to commercially pure Ti grade II, for all the markers tested. In conclusion, the molecular mechanisms of interaction between human osteoblasts and the Ti-35Nb alloy follow the principal routes of osseointegration of commercially pure Ti grade II. Porosity impaired the route of transcription factor Runx-2.