Roberto Prado - Academia.edu (original) (raw)
Papers by Roberto Prado
[Weight of uterus after ligation of ovarian branches of the uterine vessel]
Revista da Associação Médica Brasileira (1992)
This study aims to evaluate whether the blockage of ovarian irrigation from uterine vessels can c... more This study aims to evaluate whether the blockage of ovarian irrigation from uterine vessels can change the gonadal function resulting in diminished estrogen secretion and weight of uterus. An experimental model was created including 30 virgin female Wistar rats. After weight determination, animals were separated into two groups: the ligation group with 20 animals, submitted to laparotomy and ligation of the ovarian branches of the uterine blood vessels, and the control group with 10 animals submitted only to laparotomy. Sixteen weeks later the rats were weighed and euthanized, and their uteri dissected and weighed. The index between animal and uteri weights was calculated. Statistical significance was not observed when the mean uterus weights and the indexes between weight of the uterus and weight of the animals were compared in the two groups. The blockage of ovary vascularization from ovary branches of the uterine vessel did not cause uterine weight alterations.
Resultados obtidos em mulheres portadoras de endometriose pélvica avançada e mulheres com ligadura tubária, submetidas a ICSI: análise comparativa
Revista Brasileira de Ginecologia e Obstetrícia, 2005
PALAVRAS-CHAVE: Endometriose/terapia; Infertilidade feminina; Fertilização in vitro; Injeção de e... more PALAVRAS-CHAVE: Endometriose/terapia; Infertilidade feminina; Fertilização in vitro; Injeção de esperma intracitoplasmáticas; Medicina reprodutiva ... Purpose: comparative analysis of the outcome of women with advanced pelvic endometriosis and women with tubal ...
Definir el vínculo existente entre cómo se organiza una sociedad y el espacio en que se desenvuel... more Definir el vínculo existente entre cómo se organiza una sociedad y el espacio en que se desenvuelve ha sido una inquietud constante entre los encargados de configurar y gestionar los asentamientos humanos. Razones no faltan para esta preocupación. La manera en que se entiende la relación hombre-espacio es básica para la vida individual y colectiva, al condicionar desde el vestido
Effects of Calcium Ions on Titanium Surfaces for Bone Regeneration
Colloids and Surfaces B: Biointerfaces, 2015
The chemistry and topography of implant surfaces are of paramount importance for the successful t... more The chemistry and topography of implant surfaces are of paramount importance for the successful tissue integration of load-bearing dental and orthopedic implants. Here we evaluate in vitro and in vivo titanium implant surfaces modified with calcium ions (Ca(2+) surfaces). Calcium ions produce a durable chemical and nano-topographical modification of the titanium oxide interface. Time of flight secondary ion mass spectrometry examination of the outermost surface composition, shows that calcium ions in Ca(2+) surfaces effectively prevent adventitious hydrocarbon passivation of the oxide layer. In aqueous solutions Ca(2+) surfaces release within the first minute, 2/3 of the total measured Ca(2+), the rest is released over the following 85 days. Additionally, Ca(2+) surfaces significantly increase human fetal osteoblasts-like cell adhesion, proliferation and differentiation, as measured by the autocrine synthesis of osteopontin. Relevant for clinical application, after 12 weeks of healing in sheep tibia, microcomputer tomography and histomorphometric analysis show that Ca(2+) surfaces develop significantly more bone contacts and higher bone density in the 1mm region around the implant. Consequently, titanium implants modified with calcium ions represent a valuable tool to improve endosseous integration in the clinical practice.
Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 2011
You are not walking alone in the PRP consensus road
Muscles, Ligaments and Tendons Journal, 2014
Arthroscopy: The Journal of Arthroscopic & Related Surgery, 2014
Journal of Controlled Release, 2015
PRGF is a platelet concentrate within a plasma suspension that forms an in situ-generated fibrin-... more PRGF is a platelet concentrate within a plasma suspension that forms an in situ-generated fibrin-matrix delivery system, releasing multiple growth factors and other bioactive molecules that play key roles in tissue regeneration. This study was aimed at exploring the angiogenic and myogenic effects of PRGF on in vitro endothelial cells (HUVEC) and skeletal myoblasts (hSkMb) as well as on in vivo mouse subcutaneously implanted matrigel and on limb muscles after a severe ischemia. Human PRGF was prepared and characterized. Both proliferative and anti-apoptotic responses to PRGF were assessed in vitro in HUVEC and hSkMb. In vivo murine matrigel plug assay was conducted to determine the angiogenic capacity of PRGF, whereas in vivo ischemic hind limb model was carried out to demonstrate PRGF-driven vascular and myogenic regeneration. Primary HUVEC and hSkMb incubated with PRGF showed a dose dependent proliferative and anti-apoptotic effect and the PRGF matrigel plugs triggered an early and significant sustained angiogenesis compared with the control group. Moreover, mice treated with PRGF intramuscular infiltrations displayed a substantial reperfusion enhancement at day 28 associated with a fibrotic tissue reduction. These findings suggest that PRGF-induced angiogenesis is functionally effective at expanding the perfusion capacity of the new vasculature and attenuating the endogenous tissue fibrosis after a severe-induced skeletal muscle ischemia.
Endogenous morphogens and fibrin bioscaffolds for stem cell therapeutics
Trends in Biotechnology, 2013
Clinical translation of mesenchymal stem cells (MSCs) is leading to optimization of procedures fo... more Clinical translation of mesenchymal stem cells (MSCs) is leading to optimization of procedures for ex vivo expansion. Endogenous growth factors and fibrin scaffolds can be used to support MSC expansion and transplantation. Cell growth on a fibrin scaffold mimics the 3D environment of tissue and facilitates handling and subsequent transplantation. This approach is presented as an essential toolbox in the substitution of fetal bovine serum in all large-scale ex vivo processes, providing quick and safe expansion of MSCs. This paper reviews the state of the art of platelet-rich plasma technology applied to clinical use of stem cells, focusing on current technology and methods, new challenges, and controversies.
Mathematische Zeitschrift, 2008
Quantum dynamical lower bounds for continuous and discrete one-dimensional Dirac operators are es... more Quantum dynamical lower bounds for continuous and discrete one-dimensional Dirac operators are established in terms of transfer matrices. Then such results are applied to various models, including the Bernoulli-Dirac one and, in contrast to the discrete case, critical energies are also found for the continuous Dirac case with positive mass.
The type of platelet-rich plasma may influence the safety of the approach
Knee Surgery, Sports Traumatology, Arthroscopy, 2012
Journal of Physics A: Mathematical and General, 2005
An 1D tight-binding version of the Dirac equation is considered; after checking that it recovers ... more An 1D tight-binding version of the Dirac equation is considered; after checking that it recovers the usual discrete Schrödinger equation in the nonrelativistic limit, it is found that for two-valued Bernoulli potentials the zero mass case presents absence of dynamical localization for specific values of the energy, albeit it has no continuous spectrum. For the other energy values (again excluding some very specific ones) the Bernoulli Dirac system is localized, independently of the mass.
How Safe Is Your Motorcycle Helmet?
Journal of Oral and Maxillofacial Surgery, 2014
Motorcycle crash helmets do not totally prevent head and facial trauma. The aim of this study was... more Motorcycle crash helmets do not totally prevent head and facial trauma. The aim of this study was to investigate if protection offered by helmets differs according to helmet type. In this retrospective cohort study, outpatient records of motorcyclists were analyzed for the Facial Injury Severity Scale (FISS), traumatic brain injury (TBI), facial fractures, and helmet use. Statistical analysis was conducted using the Fisher and Bonferroni tests, bivariate regression analysis, and 1-way analysis of variance. There were 253 motorcyclists who sustained craniomaxillofacial injuries and were referred for outpatient treatment (men, 88.9%; mean age, 29.64 ± 11.6 yr); 60.1% had up to 9 years of formal education; 156 patients reported not using crash helmets, 51 were using open-face helmets, and 46 were using full-face helmets. The mean FISS score was significantly higher for unhelmeted riders compared with full-face helmet riders (P = .047), with no difference between unhelmeted riders and open-face helmet users (P = 1.00). Results for TBI were statistically greater for those wearing open-face helmets compared with full-face helmets (P = .035). In this study, a large percentage of motorcyclists had facial fractures and TBI, and crash helmets did not always offer adequate protection against craniomaxillofacial injury, especially open-face helmets. Thus, further investigation into helmet types and quality of protection offered is recommended.
Journal of Mathematical Physics, 2005
A 1D Dirac tight-binding model is considered and it is shown that its nonrelativistic limit is th... more A 1D Dirac tight-binding model is considered and it is shown that its nonrelativistic limit is the 1D discrete Schrödinger model. For random Bernoulli potentials taking two values (without correlations), for typical realizations and for all values of the mass, it is shown that its spectrum is pure point, whereas the zero mass case presents dynamical delocalization for specific values of the energy. The massive case presents dynamical localization (excluding some particular values of the energy). Finally, for general potentials the dynamical moments for distinct masses are compared, especially the massless and massive Bernoulli cases.
Conservative Treatment of Bilateral Temporalis Muscle Hypertrophy in a Pregnant Woman
Journal of Craniofacial Surgery, 2012
Hypertrophy of the masticatory muscles may affect the temporalis, masseter, and pterygoid muscles... more Hypertrophy of the masticatory muscles may affect the temporalis, masseter, and pterygoid muscles in several different combinations. A case of bilateral temporalis muscle hypertrophy, with masseter and pterygoid involvement, in a 26-year-old white woman in the fourth month of gestation is reported. Magnetic resonance imaging and physical examination were used for diagnosis. Hypertrophy was considered to be of acquired nature. The patient was managed using alternative, noninvasive methods. After 2 months, visible reduction was observed. Clinical symptoms of muscle discomfort were also reported. In the presented case, we were able to use an alternative, noninvasive form of treatment, which provided our patient with an overall improvement in physical and psychologic aspects.
Plasma rich in growth factors: The pioneering autologous technology for tissue regeneration
Journal of Biomedical Materials Research Part A, 2011
Comments on the paper "High doses of riboflavin and the elimination of dietary red meat promote the recovery of some motor functions in Parkinson's disease patients. C.G. Coimbraand V.B.C. Junqueira. Brazilian Journal of Medical and Biological Research, 36: 1409-1417, 2003
Brazilian Journal of Medical and Biological Research, 2004
Biology of the Cell, 2010
Background information. The execution phase of apoptosis is characterized by extensive blebbing o... more Background information. The execution phase of apoptosis is characterized by extensive blebbing of the plasma membrane, which usually results in secondary lysis in vitro. To analyse the permeability of cellular membranes during this process, we induced apoptosis in human melanoma A375 cells that had been transfected with fluorescently tagged proteins which were targeted to different subcellular locations.
Depression in Parkinson's disease: study of 60 cases
Arquivos de Neuro-Psiquiatria, 2002
Depression is very frequent in Parkinson's disease (PD) and largely unrec... more Depression is very frequent in Parkinson's disease (PD) and largely unrecognized by neurologists, emphasizing the need of an approach to psychiatric symptoms by non psychiatrists in order to ensure an early diagnosis of depression in PD; clinical characteristics and the prevalence rate of depression in PD were evaluated and the relationship of depression in PD with other variables were determined. Sixty PD subjects, who fulfilled the clinical criteria for primary PD, 56.6% males, age range from 44 to 85 years old, in different stages of the disease were investigated. All subjects were submitted to the UPDRS-III, V and VI, Clinical Interview Schedule and the Hamilton depression scale. A significant correlation was found between depression and UPDRS-III, V and VI, anxiety and irritability. The frequency of depression in PD in this study was nearly 40% possessing specific features. Structured interviews and evaluation scales are essential for an accurate diagnosis and proper treatment of depression in PD.
Synovial cyst of the thoracic spine: case report
Arquivos de Neuro-Psiquiatria, 2007
Spinal cord compressing syndrome due to synovial cyst (SC) of the thoracic spine is a rare clinic... more Spinal cord compressing syndrome due to synovial cyst (SC) of the thoracic spine is a rare clinic condition. We report a case of SC located in the thoracic spine causing spastic paraparesis in a 14 year-old female patient. The SC was removed thoroughly by laminectomy. The patient had an excellent recovery. The etiological and therapeutic aspects are discussed.
[Weight of uterus after ligation of ovarian branches of the uterine vessel]
Revista da Associação Médica Brasileira (1992)
This study aims to evaluate whether the blockage of ovarian irrigation from uterine vessels can c... more This study aims to evaluate whether the blockage of ovarian irrigation from uterine vessels can change the gonadal function resulting in diminished estrogen secretion and weight of uterus. An experimental model was created including 30 virgin female Wistar rats. After weight determination, animals were separated into two groups: the ligation group with 20 animals, submitted to laparotomy and ligation of the ovarian branches of the uterine blood vessels, and the control group with 10 animals submitted only to laparotomy. Sixteen weeks later the rats were weighed and euthanized, and their uteri dissected and weighed. The index between animal and uteri weights was calculated. Statistical significance was not observed when the mean uterus weights and the indexes between weight of the uterus and weight of the animals were compared in the two groups. The blockage of ovary vascularization from ovary branches of the uterine vessel did not cause uterine weight alterations.
Resultados obtidos em mulheres portadoras de endometriose pélvica avançada e mulheres com ligadura tubária, submetidas a ICSI: análise comparativa
Revista Brasileira de Ginecologia e Obstetrícia, 2005
PALAVRAS-CHAVE: Endometriose/terapia; Infertilidade feminina; Fertilização in vitro; Injeção de e... more PALAVRAS-CHAVE: Endometriose/terapia; Infertilidade feminina; Fertilização in vitro; Injeção de esperma intracitoplasmáticas; Medicina reprodutiva ... Purpose: comparative analysis of the outcome of women with advanced pelvic endometriosis and women with tubal ...
Definir el vínculo existente entre cómo se organiza una sociedad y el espacio en que se desenvuel... more Definir el vínculo existente entre cómo se organiza una sociedad y el espacio en que se desenvuelve ha sido una inquietud constante entre los encargados de configurar y gestionar los asentamientos humanos. Razones no faltan para esta preocupación. La manera en que se entiende la relación hombre-espacio es básica para la vida individual y colectiva, al condicionar desde el vestido
Effects of Calcium Ions on Titanium Surfaces for Bone Regeneration
Colloids and Surfaces B: Biointerfaces, 2015
The chemistry and topography of implant surfaces are of paramount importance for the successful t... more The chemistry and topography of implant surfaces are of paramount importance for the successful tissue integration of load-bearing dental and orthopedic implants. Here we evaluate in vitro and in vivo titanium implant surfaces modified with calcium ions (Ca(2+) surfaces). Calcium ions produce a durable chemical and nano-topographical modification of the titanium oxide interface. Time of flight secondary ion mass spectrometry examination of the outermost surface composition, shows that calcium ions in Ca(2+) surfaces effectively prevent adventitious hydrocarbon passivation of the oxide layer. In aqueous solutions Ca(2+) surfaces release within the first minute, 2/3 of the total measured Ca(2+), the rest is released over the following 85 days. Additionally, Ca(2+) surfaces significantly increase human fetal osteoblasts-like cell adhesion, proliferation and differentiation, as measured by the autocrine synthesis of osteopontin. Relevant for clinical application, after 12 weeks of healing in sheep tibia, microcomputer tomography and histomorphometric analysis show that Ca(2+) surfaces develop significantly more bone contacts and higher bone density in the 1mm region around the implant. Consequently, titanium implants modified with calcium ions represent a valuable tool to improve endosseous integration in the clinical practice.
Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 2011
You are not walking alone in the PRP consensus road
Muscles, Ligaments and Tendons Journal, 2014
Arthroscopy: The Journal of Arthroscopic & Related Surgery, 2014
Journal of Controlled Release, 2015
PRGF is a platelet concentrate within a plasma suspension that forms an in situ-generated fibrin-... more PRGF is a platelet concentrate within a plasma suspension that forms an in situ-generated fibrin-matrix delivery system, releasing multiple growth factors and other bioactive molecules that play key roles in tissue regeneration. This study was aimed at exploring the angiogenic and myogenic effects of PRGF on in vitro endothelial cells (HUVEC) and skeletal myoblasts (hSkMb) as well as on in vivo mouse subcutaneously implanted matrigel and on limb muscles after a severe ischemia. Human PRGF was prepared and characterized. Both proliferative and anti-apoptotic responses to PRGF were assessed in vitro in HUVEC and hSkMb. In vivo murine matrigel plug assay was conducted to determine the angiogenic capacity of PRGF, whereas in vivo ischemic hind limb model was carried out to demonstrate PRGF-driven vascular and myogenic regeneration. Primary HUVEC and hSkMb incubated with PRGF showed a dose dependent proliferative and anti-apoptotic effect and the PRGF matrigel plugs triggered an early and significant sustained angiogenesis compared with the control group. Moreover, mice treated with PRGF intramuscular infiltrations displayed a substantial reperfusion enhancement at day 28 associated with a fibrotic tissue reduction. These findings suggest that PRGF-induced angiogenesis is functionally effective at expanding the perfusion capacity of the new vasculature and attenuating the endogenous tissue fibrosis after a severe-induced skeletal muscle ischemia.
Endogenous morphogens and fibrin bioscaffolds for stem cell therapeutics
Trends in Biotechnology, 2013
Clinical translation of mesenchymal stem cells (MSCs) is leading to optimization of procedures fo... more Clinical translation of mesenchymal stem cells (MSCs) is leading to optimization of procedures for ex vivo expansion. Endogenous growth factors and fibrin scaffolds can be used to support MSC expansion and transplantation. Cell growth on a fibrin scaffold mimics the 3D environment of tissue and facilitates handling and subsequent transplantation. This approach is presented as an essential toolbox in the substitution of fetal bovine serum in all large-scale ex vivo processes, providing quick and safe expansion of MSCs. This paper reviews the state of the art of platelet-rich plasma technology applied to clinical use of stem cells, focusing on current technology and methods, new challenges, and controversies.
Mathematische Zeitschrift, 2008
Quantum dynamical lower bounds for continuous and discrete one-dimensional Dirac operators are es... more Quantum dynamical lower bounds for continuous and discrete one-dimensional Dirac operators are established in terms of transfer matrices. Then such results are applied to various models, including the Bernoulli-Dirac one and, in contrast to the discrete case, critical energies are also found for the continuous Dirac case with positive mass.
The type of platelet-rich plasma may influence the safety of the approach
Knee Surgery, Sports Traumatology, Arthroscopy, 2012
Journal of Physics A: Mathematical and General, 2005
An 1D tight-binding version of the Dirac equation is considered; after checking that it recovers ... more An 1D tight-binding version of the Dirac equation is considered; after checking that it recovers the usual discrete Schrödinger equation in the nonrelativistic limit, it is found that for two-valued Bernoulli potentials the zero mass case presents absence of dynamical localization for specific values of the energy, albeit it has no continuous spectrum. For the other energy values (again excluding some very specific ones) the Bernoulli Dirac system is localized, independently of the mass.
How Safe Is Your Motorcycle Helmet?
Journal of Oral and Maxillofacial Surgery, 2014
Motorcycle crash helmets do not totally prevent head and facial trauma. The aim of this study was... more Motorcycle crash helmets do not totally prevent head and facial trauma. The aim of this study was to investigate if protection offered by helmets differs according to helmet type. In this retrospective cohort study, outpatient records of motorcyclists were analyzed for the Facial Injury Severity Scale (FISS), traumatic brain injury (TBI), facial fractures, and helmet use. Statistical analysis was conducted using the Fisher and Bonferroni tests, bivariate regression analysis, and 1-way analysis of variance. There were 253 motorcyclists who sustained craniomaxillofacial injuries and were referred for outpatient treatment (men, 88.9%; mean age, 29.64 ± 11.6 yr); 60.1% had up to 9 years of formal education; 156 patients reported not using crash helmets, 51 were using open-face helmets, and 46 were using full-face helmets. The mean FISS score was significantly higher for unhelmeted riders compared with full-face helmet riders (P = .047), with no difference between unhelmeted riders and open-face helmet users (P = 1.00). Results for TBI were statistically greater for those wearing open-face helmets compared with full-face helmets (P = .035). In this study, a large percentage of motorcyclists had facial fractures and TBI, and crash helmets did not always offer adequate protection against craniomaxillofacial injury, especially open-face helmets. Thus, further investigation into helmet types and quality of protection offered is recommended.
Journal of Mathematical Physics, 2005
A 1D Dirac tight-binding model is considered and it is shown that its nonrelativistic limit is th... more A 1D Dirac tight-binding model is considered and it is shown that its nonrelativistic limit is the 1D discrete Schrödinger model. For random Bernoulli potentials taking two values (without correlations), for typical realizations and for all values of the mass, it is shown that its spectrum is pure point, whereas the zero mass case presents dynamical delocalization for specific values of the energy. The massive case presents dynamical localization (excluding some particular values of the energy). Finally, for general potentials the dynamical moments for distinct masses are compared, especially the massless and massive Bernoulli cases.
Conservative Treatment of Bilateral Temporalis Muscle Hypertrophy in a Pregnant Woman
Journal of Craniofacial Surgery, 2012
Hypertrophy of the masticatory muscles may affect the temporalis, masseter, and pterygoid muscles... more Hypertrophy of the masticatory muscles may affect the temporalis, masseter, and pterygoid muscles in several different combinations. A case of bilateral temporalis muscle hypertrophy, with masseter and pterygoid involvement, in a 26-year-old white woman in the fourth month of gestation is reported. Magnetic resonance imaging and physical examination were used for diagnosis. Hypertrophy was considered to be of acquired nature. The patient was managed using alternative, noninvasive methods. After 2 months, visible reduction was observed. Clinical symptoms of muscle discomfort were also reported. In the presented case, we were able to use an alternative, noninvasive form of treatment, which provided our patient with an overall improvement in physical and psychologic aspects.
Plasma rich in growth factors: The pioneering autologous technology for tissue regeneration
Journal of Biomedical Materials Research Part A, 2011
Comments on the paper "High doses of riboflavin and the elimination of dietary red meat promote the recovery of some motor functions in Parkinson's disease patients. C.G. Coimbraand V.B.C. Junqueira. Brazilian Journal of Medical and Biological Research, 36: 1409-1417, 2003
Brazilian Journal of Medical and Biological Research, 2004
Biology of the Cell, 2010
Background information. The execution phase of apoptosis is characterized by extensive blebbing o... more Background information. The execution phase of apoptosis is characterized by extensive blebbing of the plasma membrane, which usually results in secondary lysis in vitro. To analyse the permeability of cellular membranes during this process, we induced apoptosis in human melanoma A375 cells that had been transfected with fluorescently tagged proteins which were targeted to different subcellular locations.
Depression in Parkinson's disease: study of 60 cases
Arquivos de Neuro-Psiquiatria, 2002
Depression is very frequent in Parkinson's disease (PD) and largely unrec... more Depression is very frequent in Parkinson's disease (PD) and largely unrecognized by neurologists, emphasizing the need of an approach to psychiatric symptoms by non psychiatrists in order to ensure an early diagnosis of depression in PD; clinical characteristics and the prevalence rate of depression in PD were evaluated and the relationship of depression in PD with other variables were determined. Sixty PD subjects, who fulfilled the clinical criteria for primary PD, 56.6% males, age range from 44 to 85 years old, in different stages of the disease were investigated. All subjects were submitted to the UPDRS-III, V and VI, Clinical Interview Schedule and the Hamilton depression scale. A significant correlation was found between depression and UPDRS-III, V and VI, anxiety and irritability. The frequency of depression in PD in this study was nearly 40% possessing specific features. Structured interviews and evaluation scales are essential for an accurate diagnosis and proper treatment of depression in PD.
Synovial cyst of the thoracic spine: case report
Arquivos de Neuro-Psiquiatria, 2007
Spinal cord compressing syndrome due to synovial cyst (SC) of the thoracic spine is a rare clinic... more Spinal cord compressing syndrome due to synovial cyst (SC) of the thoracic spine is a rare clinic condition. We report a case of SC located in the thoracic spine causing spastic paraparesis in a 14 year-old female patient. The SC was removed thoroughly by laminectomy. The patient had an excellent recovery. The etiological and therapeutic aspects are discussed.