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Papers by Pratibha Narang
Indian Journal of Medical Microbiology, 2004
Cancer Research, 2019
Introduction: Preclinical data suggests that priming T cell immunity to mutated or overexpressed ... more Introduction: Preclinical data suggests that priming T cell immunity to mutated or overexpressed proteins can induce tumor rejection, and may potentiate the effects of checkpoint blockade. PIK3CA and p53 are the most commonly mutated genes in breast cancer, and are enriched in high-grade and metastatic tumors. Here, we evaluated the immunogenicity of neo-epitopes derived from common PIK3CA and p53 mutations. Methods: We applied a custom informatics pipeline, EpitopeHunter, to predict neo-epitopes (8-11mers) from twelve PIK3CA and ten p53 mutations restricted to thirty-nine MHC class I alleles. We selected high affinity neo-epitopes (IEDB score < 500) with predicted poor binding of the matched wild type epitope. We used the predicted peptides to stimulate PBMC from healthy donors in vitro, and measured T cell immunity by interferon-γ ELISPOT. We compared the T cell specificity of mutant vs wild type epitopes. A positive response to an epitope was defined as a response that met bot...
Antonie van Leeuwenhoek, Jan 23, 2017
During the last two decades, nontuberculous mycobacteria (NTM) have gained in importance but ther... more During the last two decades, nontuberculous mycobacteria (NTM) have gained in importance but there is still a paucity of data, particularly for environmental isolates. We studied, over a period of two years, the spatio-temporal features of NTM isolates obtained from different environmental sources in Wardha district, India. A total of 1398 samples (699 each of soil and water) were tested and 170 (12.2%) yielded NTM isolates, including 123 from soil and 47 from water samples. Out of 170 NTM isolates, 107 (63%) belonged to potentially pathogenic mycobacteria (PPM) and 63 (37%) to the less pathogenic mycobacterial (LPM) group. Overall, maximum isolation was obtained in rainy season (20.3%) followed by winter (13.5%), post rainy (8.7%) and summer seasons (5.8%). Mycobacterium fortuitum, Mycobacterium gordonae and Mycobacterium avium complex (MAC) were common isolates followed by Mycobacterium flavescens, Mycobacterium scrofulaceum, Mycobacterium simiae and Mycobacterium marinum. From so...
The Indian Journal of Pediatrics, 1974
Annals of Tropical Paediatrics, 1994
The diagnostic potential for detection of IgG to Mycobacterium tuberculosis excretory-secretory (... more The diagnostic potential for detection of IgG to Mycobacterium tuberculosis excretory-secretory (ES) antigen in childhood pulmonary and extrapulmonary tuberculosis was explored. IgG antibody to M. tuberculosis ES antigen was detected by indirect penicillinase ELISA. Twenty (80%) out of 25 pulmonary tuberculosis cases (clinically diagnosed and/or AFB-positive), five ofnine tuberculous pleural effusion cases and only six of 69 cases in the control group were positive for IgG antibody to M. tuberculosis ES antigen. Ali CSF and sera were positive for IgG antibody in 12 cases of clinically diagnosed tuberculous meningitis (TBM). Out of 35 cases in the control group for TBM, ali five cases of pyogenic meningitis but none of the 13 cases of viral encephalitis, five cases of enteric encephalopathy and 12 cases with no CNS infection were positive for anti-tubercular IgG antibody in CSF samples. Only two of them, i.e. one case of pyogenic meningitis and the other with no CNS infection, were positive for antibody in sera. The study demonstrated the potential of this assay in the diagnosis of tuberculosis in children where bacteriological confirmation is very difficult.
The Indian journal of tuberculosis
ABSTRACT
Indian pediatrics
ABSTRACT
The Indian Journal of Medical Research
Short course intermittent chemotherapy regimens for pulmonary tuberculosis were given a field tri... more Short course intermittent chemotherapy regimens for pulmonary tuberculosis were given a field trial from February 1982 to June 1986 in district Wardha, Maharashtra The symptomatic were picked up by door to door survey and newly detected cases who were culture positive were put on treatment. The regimens for the urban patients were 2SaH2R2Z2/4S2H2 (NUI) and in half of the randomly selected patients the regimen was extended by 2S2H2 (NU2). For the rural population, the regimen was 2FI2R2Z2/4H2R2 (NR1) followed by further 2H2,R2; (NR2) in the half of the patients selected randomly. Under this field trial a total of 112 patients received the regimen NU1, The percentage of patients who become sputum negative at 15 days was 44.6%, at 60 days 54.5% and at the end of treatment i.e. 180 days, 69.6%. The extension of treatment by two months (NU2) in 61 subjects did not improve the efficacy but one bacteriological failure became negative. In the regimen NR1, 217 patients were given treatment a...
The Indian journal of medical research, 1978
The Indian journal of medical research, 2005
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 2005
Rural area of Wardha district, Maharashtra State, Central India. To determine the prevalence of t... more Rural area of Wardha district, Maharashtra State, Central India. To determine the prevalence of tuberculous lymphadenitis in children aged 0-14 years in the study area and to assess factors that may contribute towards the prevalence. House to house survey of a population of 23,229 in 35 neighbouring villages with 7900 children aged 0-14 years from May 1993 to May 1994 and from March 1995 to February 1996. The prevalence of tuberculous lymphadenitis/1000 children was 4.43. The maximum prevalence was in the 5-9 years age group. The prevalence was 34 times higher in children with positive family history of tuberculosis than in those without a history. There was an association between prevalence and the living standards of the children, with a higher prevalence in families that belonged to an underprivileged social class living in thatched, improvised houses. Multiple cervical lymph nodes >2 cm and with matting and fluctuation were found to be characteristic clinical features. The pr...
The National medical journal of India
Of the weapons of mass destruction, the biological ones are the most feared and bioterrorism has ... more Of the weapons of mass destruction, the biological ones are the most feared and bioterrorism has become one of the most vicious threats to civilized society in recent times. Biological weapons have been sporadically used for centuries. Despite international regulations, there has been a global re-emergence of the threat of biological warfare. As many as 17 countries are suspected of either including or developing biological agents in their weapons programmes. In the past decade, a number of terrorist organizations with access to bioweapons technology have emerged. Current surveillance systems may be inadequate to detect biological attacks. The onset of illness is often delayed, thus the timing and location of such an event may be extremely difficult to identify. We are unfamiliar with most of the agents of biological warfare and are ill-equipped to handle the consequences of such an attack. In addition, there is no apparent coherent policy to handle a biological terrorist incident. ...
The Journal of the Association of Physicians of India, 2001
The National medical journal of India
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 1999
Ashti and Karanja tahsils, Wardha district, Maharashtra State, Central India. To find and compare... more Ashti and Karanja tahsils, Wardha district, Maharashtra State, Central India. To find and compare the prevalence of bacillary positive pulmonary tuberculosis amongst the different tribes and in the non-tribal population. Prevalence study of pulmonary tuberculosis by house-to-house survey of symptoms among tribal (n = 20596) and non-tribal (n = 93 670) populations aged 5 years and over, between September 1989 and November 1990. The prevalence of smear and/or culture-positive tuberculosis/100000 population was 133 in the tribal and 144 in the non-tribal population. The difference in prevalence of symptomatic individuals and sputum-positive cases among the tribal and the non-tribal populations was statistically significant only in the symptomatic individuals/100000 (P = 0.01). The prevalence of cases in both groups was higher in males than females; however this difference was significant only in the tribal group (P = 0.05). Only two of the 46 tribes encountered, the Mana and Pawara tri...
Indian journal of leprosy
This study was carried out in order to find out whether antineural antibodies had a role to play ... more This study was carried out in order to find out whether antineural antibodies had a role to play in perpetuating pre-existing nerve damage in leprosy. Indirect ELISA was carried out on sera from 20 leprosy patients and five normal controls using antigen prepared from peripheral nerves of a cured bacteriologically negative leprosy patient. None of the patients had significant levels of IgG antibodies whereas eight of them (40%) had significant levels of IgM antibodies. However, there was no correlation with duration of disease, treatment received, nerve enlargement or active neuritis. The nature of these antibodies is discussed.
Indian Journal of Medical Microbiology, 2004
Cancer Research, 2019
Introduction: Preclinical data suggests that priming T cell immunity to mutated or overexpressed ... more Introduction: Preclinical data suggests that priming T cell immunity to mutated or overexpressed proteins can induce tumor rejection, and may potentiate the effects of checkpoint blockade. PIK3CA and p53 are the most commonly mutated genes in breast cancer, and are enriched in high-grade and metastatic tumors. Here, we evaluated the immunogenicity of neo-epitopes derived from common PIK3CA and p53 mutations. Methods: We applied a custom informatics pipeline, EpitopeHunter, to predict neo-epitopes (8-11mers) from twelve PIK3CA and ten p53 mutations restricted to thirty-nine MHC class I alleles. We selected high affinity neo-epitopes (IEDB score < 500) with predicted poor binding of the matched wild type epitope. We used the predicted peptides to stimulate PBMC from healthy donors in vitro, and measured T cell immunity by interferon-γ ELISPOT. We compared the T cell specificity of mutant vs wild type epitopes. A positive response to an epitope was defined as a response that met bot...
Antonie van Leeuwenhoek, Jan 23, 2017
During the last two decades, nontuberculous mycobacteria (NTM) have gained in importance but ther... more During the last two decades, nontuberculous mycobacteria (NTM) have gained in importance but there is still a paucity of data, particularly for environmental isolates. We studied, over a period of two years, the spatio-temporal features of NTM isolates obtained from different environmental sources in Wardha district, India. A total of 1398 samples (699 each of soil and water) were tested and 170 (12.2%) yielded NTM isolates, including 123 from soil and 47 from water samples. Out of 170 NTM isolates, 107 (63%) belonged to potentially pathogenic mycobacteria (PPM) and 63 (37%) to the less pathogenic mycobacterial (LPM) group. Overall, maximum isolation was obtained in rainy season (20.3%) followed by winter (13.5%), post rainy (8.7%) and summer seasons (5.8%). Mycobacterium fortuitum, Mycobacterium gordonae and Mycobacterium avium complex (MAC) were common isolates followed by Mycobacterium flavescens, Mycobacterium scrofulaceum, Mycobacterium simiae and Mycobacterium marinum. From so...
The Indian Journal of Pediatrics, 1974
Annals of Tropical Paediatrics, 1994
The diagnostic potential for detection of IgG to Mycobacterium tuberculosis excretory-secretory (... more The diagnostic potential for detection of IgG to Mycobacterium tuberculosis excretory-secretory (ES) antigen in childhood pulmonary and extrapulmonary tuberculosis was explored. IgG antibody to M. tuberculosis ES antigen was detected by indirect penicillinase ELISA. Twenty (80%) out of 25 pulmonary tuberculosis cases (clinically diagnosed and/or AFB-positive), five ofnine tuberculous pleural effusion cases and only six of 69 cases in the control group were positive for IgG antibody to M. tuberculosis ES antigen. Ali CSF and sera were positive for IgG antibody in 12 cases of clinically diagnosed tuberculous meningitis (TBM). Out of 35 cases in the control group for TBM, ali five cases of pyogenic meningitis but none of the 13 cases of viral encephalitis, five cases of enteric encephalopathy and 12 cases with no CNS infection were positive for anti-tubercular IgG antibody in CSF samples. Only two of them, i.e. one case of pyogenic meningitis and the other with no CNS infection, were positive for antibody in sera. The study demonstrated the potential of this assay in the diagnosis of tuberculosis in children where bacteriological confirmation is very difficult.
The Indian journal of tuberculosis
ABSTRACT
Indian pediatrics
ABSTRACT
The Indian Journal of Medical Research
Short course intermittent chemotherapy regimens for pulmonary tuberculosis were given a field tri... more Short course intermittent chemotherapy regimens for pulmonary tuberculosis were given a field trial from February 1982 to June 1986 in district Wardha, Maharashtra The symptomatic were picked up by door to door survey and newly detected cases who were culture positive were put on treatment. The regimens for the urban patients were 2SaH2R2Z2/4S2H2 (NUI) and in half of the randomly selected patients the regimen was extended by 2S2H2 (NU2). For the rural population, the regimen was 2FI2R2Z2/4H2R2 (NR1) followed by further 2H2,R2; (NR2) in the half of the patients selected randomly. Under this field trial a total of 112 patients received the regimen NU1, The percentage of patients who become sputum negative at 15 days was 44.6%, at 60 days 54.5% and at the end of treatment i.e. 180 days, 69.6%. The extension of treatment by two months (NU2) in 61 subjects did not improve the efficacy but one bacteriological failure became negative. In the regimen NR1, 217 patients were given treatment a...
The Indian journal of medical research, 1978
The Indian journal of medical research, 2005
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 2005
Rural area of Wardha district, Maharashtra State, Central India. To determine the prevalence of t... more Rural area of Wardha district, Maharashtra State, Central India. To determine the prevalence of tuberculous lymphadenitis in children aged 0-14 years in the study area and to assess factors that may contribute towards the prevalence. House to house survey of a population of 23,229 in 35 neighbouring villages with 7900 children aged 0-14 years from May 1993 to May 1994 and from March 1995 to February 1996. The prevalence of tuberculous lymphadenitis/1000 children was 4.43. The maximum prevalence was in the 5-9 years age group. The prevalence was 34 times higher in children with positive family history of tuberculosis than in those without a history. There was an association between prevalence and the living standards of the children, with a higher prevalence in families that belonged to an underprivileged social class living in thatched, improvised houses. Multiple cervical lymph nodes >2 cm and with matting and fluctuation were found to be characteristic clinical features. The pr...
The National medical journal of India
Of the weapons of mass destruction, the biological ones are the most feared and bioterrorism has ... more Of the weapons of mass destruction, the biological ones are the most feared and bioterrorism has become one of the most vicious threats to civilized society in recent times. Biological weapons have been sporadically used for centuries. Despite international regulations, there has been a global re-emergence of the threat of biological warfare. As many as 17 countries are suspected of either including or developing biological agents in their weapons programmes. In the past decade, a number of terrorist organizations with access to bioweapons technology have emerged. Current surveillance systems may be inadequate to detect biological attacks. The onset of illness is often delayed, thus the timing and location of such an event may be extremely difficult to identify. We are unfamiliar with most of the agents of biological warfare and are ill-equipped to handle the consequences of such an attack. In addition, there is no apparent coherent policy to handle a biological terrorist incident. ...
The Journal of the Association of Physicians of India, 2001
The National medical journal of India
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 1999
Ashti and Karanja tahsils, Wardha district, Maharashtra State, Central India. To find and compare... more Ashti and Karanja tahsils, Wardha district, Maharashtra State, Central India. To find and compare the prevalence of bacillary positive pulmonary tuberculosis amongst the different tribes and in the non-tribal population. Prevalence study of pulmonary tuberculosis by house-to-house survey of symptoms among tribal (n = 20596) and non-tribal (n = 93 670) populations aged 5 years and over, between September 1989 and November 1990. The prevalence of smear and/or culture-positive tuberculosis/100000 population was 133 in the tribal and 144 in the non-tribal population. The difference in prevalence of symptomatic individuals and sputum-positive cases among the tribal and the non-tribal populations was statistically significant only in the symptomatic individuals/100000 (P = 0.01). The prevalence of cases in both groups was higher in males than females; however this difference was significant only in the tribal group (P = 0.05). Only two of the 46 tribes encountered, the Mana and Pawara tri...
Indian journal of leprosy
This study was carried out in order to find out whether antineural antibodies had a role to play ... more This study was carried out in order to find out whether antineural antibodies had a role to play in perpetuating pre-existing nerve damage in leprosy. Indirect ELISA was carried out on sera from 20 leprosy patients and five normal controls using antigen prepared from peripheral nerves of a cured bacteriologically negative leprosy patient. None of the patients had significant levels of IgG antibodies whereas eight of them (40%) had significant levels of IgM antibodies. However, there was no correlation with duration of disease, treatment received, nerve enlargement or active neuritis. The nature of these antibodies is discussed.