Pratik M Oza - Academia.edu (original) (raw)
Papers by Pratik M Oza
A probiotic is a viable microbial dietary supplement that beneficial for the host through its eff... more A probiotic is a viable microbial dietary supplement that beneficial for the host through its effects in the intestinal tract. Probiotics are widely used to prepare fermented dairy products such as yogurt or freeze-dried cultures. In the future, they may also be found in fermented vegetables and meats. Several health-related effects associated with the probiotics, including alleviation of lactose intolerance and immune enhancement, have been reported in human studies. Some evidence suggests a role for probiotics in reducing the risk of rotavirus induced diarrhea and colon cancer. Prebiotics are non-digestible food ingredients that benefit the host by selectively stimulating the growth or activity of limited number of bacteria in the colon. Work with prebiotics has been limited, and only studies involving the inulin-type fructans have generated sufficient data for evaluation regarding their possible use as functional food ingredients. At present, claims about reduction of disease ris...
Biochemistry, 2016
Materials 4ʹ,6-Diamidino-2-phenylindole (DAPI), guanosine triphosphate (GTP), phenylmethylsulphon... more Materials 4ʹ,6-Diamidino-2-phenylindole (DAPI), guanosine triphosphate (GTP), phenylmethylsulphonyl fluoride (PMSF) and Cy3-conjugated goat anti-rabbit secondary antibody were purchased from Sigma (St. Louis, MO, USA). Rabbit polyclonal FtsZ antibody was developed by Bangalore Genei (Bangalore, India). Isopropyl-β-D-thiogalactopyranoside (IPTG) was obtained from Calbiochem. Nickel-NTA and P-6 resin were procured from Qiagen (Hilden, Germany) and Bio-Rad (CA, USA) respectively. BacLight™ Bacterial Membrane Potential Kit was obtained from Invitrogen. All other reagents used in the study were of analytical grade. Methods Chemistry Experimental details for the synthesis of the novel compounds described in this paper, their spectroscopic and HPLC data is included in the supplementary information. The percentage yields only are given for those compounds which have been prepared previously and for which the physicochemical data are identical. For the preparation of some of the compounds containing phenolic groups (1e, 1f, 1k, 2d, 3d; Supplementary S1), the phenol group of the aromatic aldehyde was protected as a tetrahydropyranyl ether (THP) prior to the aldol condensation. The THP group undergoes deprotectionunder the acidic conditions employed in the final step of the reaction. General Details All glass apparatus was oven dried prior to use. All chemicals used were purchased from Aldrich Chemical Co. Ltd. (St Louis, MO, USA) and were of the highest purity commercially available. All solvents were distilled by standard techniques prior to use. Where stated, reactions were performed under an inert atmosphere of nitrogen using syringe-septum cap techniques or a
Tuberculosis is one of the most devastating bacterial disease having high rates of morbidity and ... more Tuberculosis is one of the most devastating bacterial disease having high rates of morbidity and mortality. Mycobacterium tuberculosis invades the host immune system and persists in pulmonary granulomas. Review presents bacterial cell wall or envelope structure, drug targets against M. tuberculosis and novel therapeutics agents like quinolone, pyrimidine, nitroimidazopyran and oxazolidinone derivatives. This review also focuses on the newer untouched targets like immunomodulators and isocitrate lyase inhibitors, which can be further exploited. Introduction Tuberculosis (TB) is characterized as a chronic bacterial infection caused by Mycobacterium tuberculosis, an aerobic acid-fast bacillus (AFB). Tuberculosis killed about 2 million people every year and the epidemic, which is spreading globally, is assuming alarming proportions. Around 8 million people become infected with TB every year. The WHO 'Fact Sheet' on TB estimates that between 2000 and 2020, nearly one billion peop...
Bioorganic & Medicinal Chemistry Letters, 2007
Synthesis and biological evaluation of some 5-ethoxycarbonyl-6-isopropylamino-4-(substitutedpheny... more Synthesis and biological evaluation of some 5-ethoxycarbonyl-6-isopropylamino-4-(substitutedphenyl)aminopyrimidines have been achieved by cyclization of N-[2-ethoxycarbonyl-2-cyano-1-(isopropylamino)vinyl] formamidine in presence of dry HCl in dioxane followed by nucleophilic substitution of 4-chloro group with substituted aromatic amine or phenoxide. Target compounds were evaluated for their analgesic and anti-inflammatory potential by known experimental models. Some of the compounds emerged out as more potent than standard drugs. Very low ulcer index was observed for the potent compounds.
Journal of Scientific …, 2007
Tuberculosis is one of the most devastating bacterial disease having high rates of morbidity and ... more Tuberculosis is one of the most devastating bacterial disease having high rates of morbidity and mortality. Mycobacterium tuberculosis invades the host immune system and persists in pulmonary granulomas. Review presents bacterial cell wall or envelope structure, drug targets against M. tuberculosis and novel therapeutics agents like quinolone, pyrimidine, nitroimidazopyran and oxazolidinone derivatives. This review also focuses on the newer untouched targets like immunomodulators and isocitrate lyase inhibitors, which can be further exploited.
Future Medicinal Chemistry, 2016
Filamenting temperature-sensitive mutant Z (FtsZ), an essential cell division protein in bacteria... more Filamenting temperature-sensitive mutant Z (FtsZ), an essential cell division protein in bacteria, has recently emerged as an important and exploitable antibacterial target. Cytokinesis in bacteria is regulated by the assembly dynamics of this protein, which is ubiquitously present in eubacteria. The perturbation of FtsZ assembly has been found to have a deleterious effect on the cytokinetic machinery and, in turn, upon cell survival. FtsZ is highly conserved among prokaryotes, offering the possibility of broad-spectrum antibacterial agents, while its limited sequence homology with tubulin (an essential protein in eukaryotic mitosis) offers the possibility of selective toxicity. This review aims to summarize current knowledge regarding the mechanism of action of FtsZ, and to highlight existing attempts toward the development of clinically useful inhibitors.
A probiotic is a viable microbial dietary supplement that beneficial for the host through its eff... more A probiotic is a viable microbial dietary supplement that beneficial for the host through its effects in the intestinal tract. Probiotics are widely used to prepare fermented dairy products such as yogurt or freeze-dried cultures. In the future, they may also be found in fermented vegetables and meats. Several health-related effects associated with the probiotics, including alleviation of lactose intolerance and immune enhancement, have been reported in human studies. Some evidence suggests a role for probiotics in reducing the risk of rotavirus induced diarrhea and colon cancer. Prebiotics are non-digestible food ingredients that benefit the host by selectively stimulating the growth or activity of limited number of bacteria in the colon. Work with prebiotics has been limited, and only studies involving the inulin-type fructans have generated sufficient data for evaluation regarding their possible use as functional food ingredients. At present, claims about reduction of disease ris...
Biochemistry, 2016
Materials 4ʹ,6-Diamidino-2-phenylindole (DAPI), guanosine triphosphate (GTP), phenylmethylsulphon... more Materials 4ʹ,6-Diamidino-2-phenylindole (DAPI), guanosine triphosphate (GTP), phenylmethylsulphonyl fluoride (PMSF) and Cy3-conjugated goat anti-rabbit secondary antibody were purchased from Sigma (St. Louis, MO, USA). Rabbit polyclonal FtsZ antibody was developed by Bangalore Genei (Bangalore, India). Isopropyl-β-D-thiogalactopyranoside (IPTG) was obtained from Calbiochem. Nickel-NTA and P-6 resin were procured from Qiagen (Hilden, Germany) and Bio-Rad (CA, USA) respectively. BacLight™ Bacterial Membrane Potential Kit was obtained from Invitrogen. All other reagents used in the study were of analytical grade. Methods Chemistry Experimental details for the synthesis of the novel compounds described in this paper, their spectroscopic and HPLC data is included in the supplementary information. The percentage yields only are given for those compounds which have been prepared previously and for which the physicochemical data are identical. For the preparation of some of the compounds containing phenolic groups (1e, 1f, 1k, 2d, 3d; Supplementary S1), the phenol group of the aromatic aldehyde was protected as a tetrahydropyranyl ether (THP) prior to the aldol condensation. The THP group undergoes deprotectionunder the acidic conditions employed in the final step of the reaction. General Details All glass apparatus was oven dried prior to use. All chemicals used were purchased from Aldrich Chemical Co. Ltd. (St Louis, MO, USA) and were of the highest purity commercially available. All solvents were distilled by standard techniques prior to use. Where stated, reactions were performed under an inert atmosphere of nitrogen using syringe-septum cap techniques or a
Tuberculosis is one of the most devastating bacterial disease having high rates of morbidity and ... more Tuberculosis is one of the most devastating bacterial disease having high rates of morbidity and mortality. Mycobacterium tuberculosis invades the host immune system and persists in pulmonary granulomas. Review presents bacterial cell wall or envelope structure, drug targets against M. tuberculosis and novel therapeutics agents like quinolone, pyrimidine, nitroimidazopyran and oxazolidinone derivatives. This review also focuses on the newer untouched targets like immunomodulators and isocitrate lyase inhibitors, which can be further exploited. Introduction Tuberculosis (TB) is characterized as a chronic bacterial infection caused by Mycobacterium tuberculosis, an aerobic acid-fast bacillus (AFB). Tuberculosis killed about 2 million people every year and the epidemic, which is spreading globally, is assuming alarming proportions. Around 8 million people become infected with TB every year. The WHO 'Fact Sheet' on TB estimates that between 2000 and 2020, nearly one billion peop...
Bioorganic & Medicinal Chemistry Letters, 2007
Synthesis and biological evaluation of some 5-ethoxycarbonyl-6-isopropylamino-4-(substitutedpheny... more Synthesis and biological evaluation of some 5-ethoxycarbonyl-6-isopropylamino-4-(substitutedphenyl)aminopyrimidines have been achieved by cyclization of N-[2-ethoxycarbonyl-2-cyano-1-(isopropylamino)vinyl] formamidine in presence of dry HCl in dioxane followed by nucleophilic substitution of 4-chloro group with substituted aromatic amine or phenoxide. Target compounds were evaluated for their analgesic and anti-inflammatory potential by known experimental models. Some of the compounds emerged out as more potent than standard drugs. Very low ulcer index was observed for the potent compounds.
Journal of Scientific …, 2007
Tuberculosis is one of the most devastating bacterial disease having high rates of morbidity and ... more Tuberculosis is one of the most devastating bacterial disease having high rates of morbidity and mortality. Mycobacterium tuberculosis invades the host immune system and persists in pulmonary granulomas. Review presents bacterial cell wall or envelope structure, drug targets against M. tuberculosis and novel therapeutics agents like quinolone, pyrimidine, nitroimidazopyran and oxazolidinone derivatives. This review also focuses on the newer untouched targets like immunomodulators and isocitrate lyase inhibitors, which can be further exploited.
Future Medicinal Chemistry, 2016
Filamenting temperature-sensitive mutant Z (FtsZ), an essential cell division protein in bacteria... more Filamenting temperature-sensitive mutant Z (FtsZ), an essential cell division protein in bacteria, has recently emerged as an important and exploitable antibacterial target. Cytokinesis in bacteria is regulated by the assembly dynamics of this protein, which is ubiquitously present in eubacteria. The perturbation of FtsZ assembly has been found to have a deleterious effect on the cytokinetic machinery and, in turn, upon cell survival. FtsZ is highly conserved among prokaryotes, offering the possibility of broad-spectrum antibacterial agents, while its limited sequence homology with tubulin (an essential protein in eukaryotic mitosis) offers the possibility of selective toxicity. This review aims to summarize current knowledge regarding the mechanism of action of FtsZ, and to highlight existing attempts toward the development of clinically useful inhibitors.