Priscila Lora - Academia.edu (original) (raw)

Papers by Priscila Lora

Annals of the Rheumatic Diseases, 2013

ABSTRACT Background Rheumatoid arthritis (RA) is an inflammatory disease with autoimmune manifest... more ABSTRACT Background Rheumatoid arthritis (RA) is an inflammatory disease with autoimmune manifestations characterized by chronic inflammation of the joints associated with systemic complications [1]. Systemic inflammation of RA causes metabolic changes and deregulation of leptin, adiponectin and insulin, leading to anorexia, changes in normal food intake and weight loss [2]. However, observational with human subjects have many potential bias and animal models can be a good alternative for studies on the effect of chronic arthritis in metabolic changes and feeding preferences. Objectives To evaluate the feeding preferences of rats with type II collagen induced arthritis (CIA). Methods Female Wistar rats were separated in two groups: control (CO) and collagen-induced arthritis (CIA) performed accordingly to Rosloniec et al. [3] with modifications (without booster). Arthritis clinical score (0-16) of animals was registered three times a week for analysis of the development of the disease [3]. All animals were offered four different diets at the same time: control diet (standard ration - CD), high calorie diet (addition of 5% of saccharose – HCD), high protein diet (addition of 5% of albumin – HPD) and high fat diet (addition of 5% of soybean oil – HFD). The animals and ration leftovers were weighted every three days for 14 days before and 25 days after the induction of arthritis. Comparison between groups was performed by two-way ANOVA followed by Tukey post-hoc test. Results The first signs of arthritis appeared by 14 days after induction (score 2.7±3.1) and the inflammatory peak appeared to be around day 17 to day 22 (score 9.8±4.1). Food consumption of control and CIA rats before the onset of arthritis was: 45% of HCD (51.9±6.4 g), 36% of HFD (41.6±9.7 g), 13% of HPD (15.3±5.1 g) and 7% of CD (7.5±5.8 g). During the peak of arthritis, CIA rats had a significant weight loss (-14.7±8.5 g) while CO group maintained their weight (-0.2±4.6 g, p<.01). At the same time, CIA had reduced total food intake (72.3 ± 8.6 g) compared to CO (107.3±5.7 g, p<.01). Besides that, CIA changed the food preference, increasing the intake of HPD to 20% (16.3±5.3 g, p<.05) and reducing the ingestion of the HCD to 27% (22.6±7.8 g, p<.05) compared to CO (10.0±1.5 g and 58.3±21.3 g, respectively). Conclusions CIA animals demonstrated changes in their food intake, especially during the inflammatory peak of arthritis, with concomitant reduction in weight. These changes include decreased total food consumption and different food preferences, like increased intake of high-protein diet and reduced intake of high-calorie diet, results never reported before. There is still needed more studies about the feeding preferences and intake, but CIA model could be a good model to study altered food consumption caused by arthritis and hereafter correlate these findings with alterations in RA patients. Acknowledgements FIPE-HCPA, CAPES, CNPq Disclosure of Interest None Declared

Background: Rheumatoid arthritis (RA) is a systemic inflammatory autoimmune disease of unknown et... more Background: Rheumatoid arthritis (RA) is a systemic inflammatory autoimmune disease of unknown etiology. Animal models of arthritis are extremely useful to investigate the pathophysiology of the disease and its new therapies. Aim: Considering that RA affects predominantly women, and acknowledging the lack of studies about the influence of gender in the development of experimental arthritis, the objective of this study was to assess the impact of gender on the clinical development of type II collagen-induced arthritis (CIA). Methods: Animals were divided in two groups (male and female), both n = 6. CIA was induced using intradermal injections with collagen on day zero and 18. Clinical score of arthritis and joint edema were evaluated daily for 10 days after the onset of the disease. Results: Clinical score showed no difference between males and females. The clinical score assessed separately – front and rear paws, showed a significant difference (p<0.001) – rear paws on day 5 (mal...

Revista Brasileira de Reumatologia

Rheumatoid arthritis (RA) is an autoimmune disease associated with polyarticular inflammatory syn... more Rheumatoid arthritis (RA) is an autoimmune disease associated with polyarticular inflammatory synovitis affecting mainly peripheral joints. It affects approximately 1% of the world population, being two to three times more prevalent in women. Rheumatoid arthritis has a complex and multifactorial pathogenesis. The synovium of the affected joints is infiltrated by T and B lymphocytes, macrophages, and granulocytes. The rheumatoid synovium has proliferative characteristics, forming the pannus, which invades cartilage and bone, leading to normal architecture destruction and function loss. The decreased expression of complement regulatory proteins (CRP) seems to play an important role in RA activity, and is associated with worsening of the clinical symptoms. In several models of autoimmune diseases, the overactivation of the complement system (CS) is the cause of disease exacerbation. This article aimed at reviewing the main aspects related to CS regulation in RA in order to provide a be...

Clinical laboratory, 2011

Different methods for anti-ENA identification have been used. This can lead to confusion regardin... more Different methods for anti-ENA identification have been used. This can lead to confusion regarding the interpretation of the test results in clinical practice. Some studies have reported differences in sensitivity and specificity, but few compare clinical outcomes. Based on that, our aim was to compare the performance characteristics of various methods commonly used to detect anti-ENA antibodies in the sera of patients suspected to have connective tissue diseases (CTDs). 189 patients with orders for anti-ENA were analyzed. Three common methods were used: DID, ELISA, and HA. Sensitivity, specificity, PPV, NPV, and LR were calculated using CTDs as the reference standard. 69.3% of the patients had a CTD and 32.8% had SLE. Sensitivity and specificity, respectively, according to the technique were: ELISA (50.0% - 78.9%); DID (31.3% - 89.5%); HA (40.9% - 87.7%). PPV were: 88.5% (HA), 87.2% (DID) and 84.6% (ELISA), and NPV were: 40.5% (ELISA), 39.1% (HA) and 36.2% (DID). Based on the very ...

Revista Brasileira de Reumatologia, 2009

Open Journal of Rheumatology and Autoimmune Diseases, 2014

The open rheumatology journal, 2013

Equisetum giganteum is a plant used in traditional medicine as diuretic. From our knowledge this ... more Equisetum giganteum is a plant used in traditional medicine as diuretic. From our knowledge this is the first time this plant is tested in an in vivo model of acute inflammation. To evaluate the effect of aqueous extract of giant horsetail (AEGH) as immunomodulatory therapy, antigen-induced arthritis (AIA) was generated in mice with methylated bovine serum albumin (mBSA). Inflammation was evaluated by articular nociception, leukocytes migration and lymphocyte proliferation. AEGH reduced nociception at 3, 6 and 24 h (P < 0.01), decreased leukocyte migration (P < 0.015), and inhibited lymphocyte proliferation stimulated with Concanavalin A and Lipopolysaccharide (P < 0.05). In conclusion, AEGH has an anti-inflammatory potential in acute model of inflammation, as well as immunomodulatory effect on both B and T lymphocytes, with an action independent of cytotoxicity.

Revista Brasileira de Reumatologia, 2007

World Journal of Stem Cells, 2012

AIM: To investigate the interaction between mesenchymal stem cells (MSCs) and bone grafts using t... more AIM: To investigate the interaction between mesenchymal stem cells (MSCs) and bone grafts using two different cultivation methods: static and dynamic.

Clinical and Developmental Immunology, 2012

CD55, CD59, CD46, and CD35 are proteins with complement regulatory (Creg) properties that ensure ... more CD55, CD59, CD46, and CD35 are proteins with complement regulatory (Creg) properties that ensure cell and tissue integrity when this system is activated. The aim of this study was to evaluate the Creg expression on peripheral blood cells from SLE patients and its association with cytopenia and disease activity. Flow cytometric analyses were performed on blood cells from 100 SLE patients and 61 healthy controls. Compared with healthy controls, we observed in SLE patients with lymphopenia and neutropenia decreased expression of CD55, CD59, and CD46 (P<0.05). In SLE patients with anemia, CD59 and CD35 were decreased on red blood cells. Furthermore, there was a negative correlation between CD55 and CD59 on neutrophils and the disease activity. The results suggest there is an altered pattern of Creg expression on the peripheral blood cells of SLE patients, and the expression is correlated with disease activity and/or with activation of the complement system.

Brazilian Journal of Microbiology, 2012

Guidelines from CDC recommend universal screening of pregnant women for rectovaginal GBS coloniza... more Guidelines from CDC recommend universal screening of pregnant women for rectovaginal GBS colonization. The objective of this study was to compare the performance of a combined enrichment/PCR based method targeting the atr gene in relation to culture using enrichment with selective broth medium (standard method) to identify the presence of GBS in pregnant women. Rectovaginal GBS samples from women at 36 weeks of pregnancy were obtained with a swab and analyzed by the two methods. A total of 89 samples were evaluated. The prevalence of positive results for GBS detection was considerable higher when assessed by the combined enrichment/PCR method than with the standard method (35.9% versus 22.5%, respectively). The results demonstrated that the use of selective enrichment broth followed by PCR targeting the atr gene is a highly sensitive, specific and accurate test for GBS screening in pregnant women, allowing the detection of the bacteria even in lightly colonized patients. This PCR methodology may provide a useful diagnostic tool for GBS detection and contributes for a more accurate and effective intrapartum antibiotic and lower newborn mortality and morbidity.

Journal of Cachexia, Sarcopenia and Muscle, 2013

Background Rheumatoid arthritis (RA) is an inflammatory autoimmune disease of unknown etiology, a... more Background Rheumatoid arthritis (RA) is an inflammatory autoimmune disease of unknown etiology, affecting mainly the joint but also other tissues. RA patients usually present weakness and muscle atrophy, nonarticular manifestations of the disease. Although causing great impact, the understanding of muscle atrophy, its development, and the mechanisms involved is still very limited. The objective of this study is to evaluate the development of muscle atrophy in skeletal muscle of a murine model of arthritis.