Puji Lestari - Academia.edu (original) (raw)
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Papers by Puji Lestari
Molecular Plant Pathology, 2006
SUMMARYTo understand the molecular basis of symbiotic association, a cDNA‐AFLP technique was used... more SUMMARYTo understand the molecular basis of symbiotic association, a cDNA‐AFLP technique was used to identify differentially expressed transcripts between a supernodulating soybean mutant, SS2‐2, and its wild‐type, Sinpaldalkong 2. As sources of cDNA‐AFLP templates, trifoliates of 2‐week‐old plants were collected 1 week after Bradyrhizobium japonicum inoculation. A total of 147 bands out of 4000 amplicons were recognized as differentially expressed fragments, with 40 transcript‐derived fragments (TDFs) in SS2‐2 and 65 TDFs in Sinpaldalkong 2. Qualitative and quantitative real‐time RT‐PCR assays suggested that the expression patterns of genes in both genotypes were clearly differentiated. TDFs homologous to nodulin (65S2) and a putative senescence‐associated protein (9S1) were up‐regulated in SS2‐2, whereas Sinpaldalkong 2 showed up‐regulation of a receptor‐like kinase (48sin1) and a kinase‐like protein (17sin1). This indicates that different genes may be involved in regulation of th...
Because the authors see in the implementation of work agreements there are still shortcomings or ... more Because the authors see in the implementation of work agreements there are still shortcomings or mismatches betwen written agreements with their implementation, therefore, the authors are interested in examining work agreements between employees and PT. Sumber Alfaria Trijaya.Tbk Penelitian ini bertujuan : 1. Untuk mengatahui lebih jauh sampai dimana pelaksanaan perjanjian kerja dilaksanakan dan dipatuhi oleh para pihak khususnya pada perusahaan PT. Sumber Alfaria Trijaya.Tbk. 2. Untuk mengatahui tentang hambatan-hambatan apa yang timbul dalam pelaksanaan perjanjian kerja di PT. Sumber Alfaria Trijaya.Tbk dan bagaimana cara penyelesaiannya. Jenis penelitian ini menggunakan penelitian lapangan (field research) yaitu penelitian yang menggunakan data primer. Sumber datanya dapat diperoleh melalui observasi, wawancara, dan partipasi pendekatan yang akan digunakan untuk memecahkan dalam penelitian ini adalah menggunakan pendekatan doktrinal . Pendekatan doktrinal yaitu penelitian yang be...
Molecular Plant Pathology, 2006
SUMMARYTo understand the molecular basis of symbiotic association, a cDNA‐AFLP technique was used... more SUMMARYTo understand the molecular basis of symbiotic association, a cDNA‐AFLP technique was used to identify differentially expressed transcripts between a supernodulating soybean mutant, SS2‐2, and its wild‐type, Sinpaldalkong 2. As sources of cDNA‐AFLP templates, trifoliates of 2‐week‐old plants were collected 1 week after Bradyrhizobium japonicum inoculation. A total of 147 bands out of 4000 amplicons were recognized as differentially expressed fragments, with 40 transcript‐derived fragments (TDFs) in SS2‐2 and 65 TDFs in Sinpaldalkong 2. Qualitative and quantitative real‐time RT‐PCR assays suggested that the expression patterns of genes in both genotypes were clearly differentiated. TDFs homologous to nodulin (65S2) and a putative senescence‐associated protein (9S1) were up‐regulated in SS2‐2, whereas Sinpaldalkong 2 showed up‐regulation of a receptor‐like kinase (48sin1) and a kinase‐like protein (17sin1). This indicates that different genes may be involved in regulation of th...
Because the authors see in the implementation of work agreements there are still shortcomings or ... more Because the authors see in the implementation of work agreements there are still shortcomings or mismatches betwen written agreements with their implementation, therefore, the authors are interested in examining work agreements between employees and PT. Sumber Alfaria Trijaya.Tbk Penelitian ini bertujuan : 1. Untuk mengatahui lebih jauh sampai dimana pelaksanaan perjanjian kerja dilaksanakan dan dipatuhi oleh para pihak khususnya pada perusahaan PT. Sumber Alfaria Trijaya.Tbk. 2. Untuk mengatahui tentang hambatan-hambatan apa yang timbul dalam pelaksanaan perjanjian kerja di PT. Sumber Alfaria Trijaya.Tbk dan bagaimana cara penyelesaiannya. Jenis penelitian ini menggunakan penelitian lapangan (field research) yaitu penelitian yang menggunakan data primer. Sumber datanya dapat diperoleh melalui observasi, wawancara, dan partipasi pendekatan yang akan digunakan untuk memecahkan dalam penelitian ini adalah menggunakan pendekatan doktrinal . Pendekatan doktrinal yaitu penelitian yang be...