Q. Shi - Academia.edu (original) (raw)

Papers by Q. Shi

Proceedings 2006 IEEE International Conference on Robotics and Automation, 2006. ICRA 2006., 2006

This paper introduces a novel robotic range sensor planning system, which is developed for 3D dim... more This paper introduces a novel robotic range sensor planning system, which is developed for 3D dimensional inspection of automotive body parts. For active, triangulation-based range sensors, shadow and reflection will cause problems when measure a metal part with glossy and discontinuous surface. To solve these problems, a feedback based dynamic view planning system is proposed, which not only generate viewpoints from a CAD model of a part, but also recursively add viewpoints according to the measured information. The planning process stops only if the obtained point clouds meet the pre-determined requirements. General framework of the system is introduced, and the experimental results are also presented.

Chinese medical journal, 1982

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2010 IEEE International Conference on Robotics and Automation, 2010

ABSTRACT Three dimensional optic measurement system's accuracy is highly related with the... more ABSTRACT Three dimensional optic measurement system's accuracy is highly related with the field of inspection. Increasing of field inspection costs increasing camera / projector pixel area on the test surface. Small surface changes within one pixel area cannot be directly detected, which will lower the system accuracy. A pixel-to-pixel strategy is developed to solve this problem. Increasing field of inspection also costs a longer standoff distance. The random image noise from the environment, uncertainties functions by lens distortion and resolution variation are all amplified. Therefore, a more complicated calibration model for each pixel is proposed to calibrate the system. In traditional structured light vision systems, a single sensor usually detects around 10,000 - 50,000 mm2, and the 3D vision sensor in this paper needs to detect around 2,400,000 mm2. Larger detection range gives more challenge to finish the calibration tasks. This paper proposes a clear calibration procedure to a large field of inspection structured light system. Last the comparison with the CMM measured results is used to prove that the calibration tasks have been successfully achieved.

2005 IEEE/RSJ International Conference on Intelligent Robots and Systems, 2005

AbstractThis paper presents new calibration methods for a robotic area sensing system developed ... more AbstractThis paper presents new calibration methods for a robotic area sensing system developed for industrial man-ufacturing inspection. A pixel-to-pixel calibration scheme is introduced to obtain standoff and baseline distance of the developed area sensor. In the three-dimensional ...

Nature communications, 2014

Mudskippers are amphibious fishes that have developed morphological and physiological adaptations... more Mudskippers are amphibious fishes that have developed morphological and physiological adaptations to match their unique lifestyles. Here we perform whole-genome sequencing of four representative mudskippers to elucidate the molecular mechanisms underlying these adaptations. We discover an expansion of innate immune system genes in the mudskippers that may provide defence against terrestrial pathogens. Several genes of the ammonia excretion pathway in the gills have experienced positive selection, suggesting their important roles in…

Protein Expression and Purification, 1996

in a developmentally regulated manner; the cloning and molecular characterizations of the corresp... more in a developmentally regulated manner; the cloning and molecular characterizations of the corresponding The adult isoform of human cardiac troponin T (TnT) contains 288 amino acids, 14 of which (4.9%) are cDNA revealed that these isoforms are generated by encoded by the rarely used arginine codons (12 AGG, alternative splicing of a single gene localized to 1q32 2 AGA) in Escherichia coli genes. To generate sufficient (2)(3)(4)(5). Recent clinical studies have found that serum quantity of TnT protein for antibody production, we cardiac TnT is a sensitive and specific marker for myocloned the corresponding cDNA and expressed it in E. cardial cell damage and that its detection can be used coli. A low-level expression of TnT that comprised only to identify, among patients with unstable angina, those about 1% of total cell protein was initially observed at risk of myocardial infarction (6,7). with the use of the native cDNA. The existence of two

Tissue Antigens, 2004

OX40 ligand (OX40L), a molecule originally identified as human gp34, is an important co-stimulato... more OX40 ligand (OX40L), a molecule originally identified as human gp34, is an important co-stimulatory molecule during immune response. In this study, we report on five functional mouse anti-human OX40L monoclonal antibodies named as 9H10, 4C12, 8D10, 4H4 and 1G1, characterized by means of flow cytometry, Western blot and competition assay. These monoclonal antibodies bound to distinct OX40L epitopes on activated B cells and dendritic cells (DCs) and two of them could suppress the proliferation of T lymphocytes co-stimulated by mature DCs. Furthermore, we demonstrated that our monoclonal antibodies, such as 9H10 and 4C12, could trigger OX40L reverse signal that enhanced IgG production of B cells and promoted maturation of DCs as evidenced by the upexpression of CD80, CD86, CD83 and CXCR4 and monoclonal antibody 9H10 could also promote anti-CD40 monoclonal-antibody-stimulated DCs in order to induce T cells to secrete more interleukin-2 and interferon-gamma, which suggested that OX40L signals could strengthen the effect of CD40 signals on promoting Th1 differentiation.

PLoS ONE, 2012

Auto-reactive B lymphocytes and its abnormal CD40 signaling play important roles in the pathogene... more Auto-reactive B lymphocytes and its abnormal CD40 signaling play important roles in the pathogenesis of systemic lupus erythematosus (SLE). In this study, we analyzed CD40 expression and CD40/CD154 induced activation of NF-kB signaling pathway in B cells from SLE patients. B cells from healthy volunteers and tonsilar B cells from chronic tonsillitis were used as negative and positive controls. Results showed CD40-induced NF-kB signaling was constitutively activated in B cells from active lupus patients, including decreased CD40 in raft portion, increased phosphorylation and degradation of IkBa, phosphorylation of P65, as well as increased nuclear translocation of P65, P50, c-Rel, which could be blocked by anti-CD154. CD154 stimulation could induce further phosphorylation and degradation of IkBa, as well as phosphorylation of P65 and nuclear translocation of P65. In addition, CD40-induced kinase activities in B cells from lupus patients mimicked that of tonsil B cells, in that IKKa/b were more activated compared to normal B cells. CD40-induced NF-kB activity was blocked by both IkB phosphorylation and proteosome degradation inhibitors in both lupus and normal B cells. All together, our findings revealed that canonical NF-kB signaling is constitutively activated in active lupus and is mediated by CD154/CD40. CD40 induced NF-kB activation is different in human lupus B lymphocytes compared with normal B cells.

PLoS ONE, 2008

Mammalian sperm capacitation is an essential prerequisite to fertilizion. Although progress had b... more Mammalian sperm capacitation is an essential prerequisite to fertilizion. Although progress had been made in understanding the physiology and biochemistry of capacitation, little is known about the potential roles of epididymal proteins during this process. Here we report that HongrES1, a new member of the SERPIN (serine proteinase inhibitor) family exclusively expressed in the rat cauda epididymis and up-regulated by androgen, is secreted into the lumen and covers the sperm head. Co-culture of caudal sperms with HongrES1 antibody in vitro resulted in a significant increase in the percentage of capacitated spermatozoa. Furthermore, the percentage of capacitated spermatozoa clearly increased in rats when HongrES1 was down-regulated by RNAi in vivo. Remarkably, knockdown of HongrES1 in vivo led to reduced fertility accompanied with deformed appearance of fetuses and pups. These results identify HongrES1 as a novel and critical molecule in the regulation of sperm capacitation and male fertility.

Mutagenesis, 2011

In mice, the occurrence of immunofluorescent foci for mismatch repair protein MLH1 correlates clo... more In mice, the occurrence of immunofluorescent foci for mismatch repair protein MLH1 correlates closely with the occurrence of crossovers, as detected genetically, and MLH1 foci represent virtually all prospective crossover positions. To examine the effects of g-irradiation on meiotic crossovers in mouse spermatocytes, male mice were subjected to wholebody g-irradiation at different sub-stages of meiotic prophase and crossovers on synaptonemal complexes (SCs) were analysed by visualising and quantifying the immunofluorescent MLH1 foci. At both 24 and 48 h after exposure, significant dose-dependent increases in the number of total MLH1 foci per spermatocyte were observed at late zygotene-early pachytene with the gradient increase of radiation dose from 0, 1.5, 3-6 Gy. Furthermore, irradiation at preleptoteneleptotene still led to significant dose-dependent increased meiotic crossovers in the spermatocytes analysed 120 h after exposure. In further analysis, these dose-dependent increases in the number of total MLH1 foci per cell were attributed to significant dose-dependent decreases in autosomal SCs with 0 MLH1 focus, and the dose-dependent increases in autosomal SCs with 2 MLH1 foci and the percentage of cells with MLH1 focus on XY bivalent. The increased number of cells with an MLH1 focus on the pseudoautosomal regions (PARs) may indicate that there is a delay in meiotic progression in the irradiated cells. Although significant dose-dependent increases in the number of total MLH1 foci per cell were examined 24, 48 or 120 h after exposure with the gradient increase of radiation doses, these increases were mild compared to the control groups. This suggests that there is tight control of crossover formation (at least with respect to MLH1 foci number). The mechanisms underlying irradiationinduced DNA lesion repair, cellular responses independent of DNA damage and meiotic crossover homeostasis in mammals will be the subjects of future study.

Letters in Applied Microbiology, 2014

Previous studies have shown that isoimperatorin (IO), a furanocoumarin isolated from several medi... more Previous studies have shown that isoimperatorin (IO), a furanocoumarin isolated from several medicinal plants, has antimycobacterial activity against Mycobacterium tuberculosis strain H37Rv (ATCC 27294). This study demonstrated that IO has antimycobacterial activity against 2 drug-sensitive and 6 drug-resistant isolates, with minimum inhibitory concentrations (MICs) of 50-100 μg ml(-1) and 100-200 μg ml(-1), respectively. IO exhibited synergistic antimycobacterial effects with rifampin (RMP), isoniazid (INH) and ethambutol (EMB) against 6 drug-resistant strains, with fractional inhibitory concentration index (FICI) values of 0·133-0·472, 0·123-0·475 and 0·124-0·25, respectively. The IO/RMP, IO/INH and IO/EMB combination treatments had synergistic effects or no interaction in the 2 drug-sensitive strains and the standard strain ATCC 27294. The synergism of combined drugs against drug-resistant strains was better than drug-sensitive strains. No antagonism was observed in with the aforementioned combinations against all strains tested. IO exhibited relatively low cytotoxicity to Vero cells. Our results indicate that IO may serve as promising a template for future antimycobacterial drug development. This is the first report on the in vitro synergistic antimycobacterial effects of isoimperatorin (IO) in combination with three first-line drugs: rifampin (RMP), isoniazid (INH) and ethambutol (EMB). The results indicated that the antimycobacterial activity of IO was modest; however, IO was a useful and effective agent against Myco. tuberculosis when it was combined with first-line antimycobacterial drugs and is worthy of further development as a lead compound for the development of novel antimycobacterial therapeutic agents.

Journal of Parenteral and Enteral Nutrition, 1991

Wistar rats were divided into two groups, intraperitoneal sepsis group (group IS) and total paren... more Wistar rats were divided into two groups, intraperitoneal sepsis group (group IS) and total parenteral nutrition group (group TPN), to evaluate the characteristics of pathologic alterations in rats with cholestasis. Biochemical assay showed that cholestasis developed in both groups after 10 days. Light microscopic examination of liver specimens revealed that the degeneration in the intermediate and external zone of hepatolobules was the major alteration in group IS, and group TPN showed characteristic dilation of central veins and hepatic sinuses and the proliferation of Kupffer cells with marked phagocytosis. Electron microscopic pictures presented the enlargement of bile canaliculi with altered microvilli in group IS and many highly electron-dense bile particles within cytoplasm and secondary lysosomes near dilated bile canaliculi in group TPN. It is concluded that there were different histopathologic alterations of liver specimens in TPN-supported animals and septic animals when cholestasis developed. It is unsuitable to take intraperitoneal sepsis as a unique factor of cholestasis in TPN-supported rats. Bile stasis is only one sign of TPN-induced hepatic lesion, which needs further exploration to determine its causes and mechanisms.

Journal of Controlled Release, 2007

A novel amphiphilic polymer-paclitaxel conjugate P(LGG-paclitaxel)-PEG-P(LGG-paclitaxel) has been... more A novel amphiphilic polymer-paclitaxel conjugate P(LGG-paclitaxel)-PEG-P(LGG-paclitaxel) has been prepared. It was derived from its parent polymer P(LGG)-PEG-P(LGG), poly{(lactic acid)-co-[(glycolic acid)-alt-(L-glutamic acid)]}-block-poly(ethylene glycol)-block-poly {(lactic acid)-co-[(glycolic acid)-alt-(L-glutamic acid)]}, which was prepared by ring-opening copolymerization of L-lactide (LLA) with (3s)benzoxylcarbonylethyl-morpholine-2,5-dione (BEMD) in the presence of dihydroxyl PEG with molecular weight of 4600 as a macroinitiator using stannous octoate (Sn(Oct) 2 ) as catalyst, and by subsequent catalytic hydrogenation. It could self-assemble into micelles in an aqueous system with P(LGG-paclitaxel) block in the core and PEG in the shell. ESEM and DLS analysis of the micelles revealed a homogeneous spherical morphology and a unimodal size distribution. In vitro release of paclitaxel from the conjugate micelles showed that its release rate depended on pH value and was higher at lower pH than in neutral condition. In vitro antitumor activity of the paclitaxel conjugate against rat brain glioma C6 cells was evaluated by MTT method. The results showed that the paclitaxel can be released from the conjugate without losing cytotoxicity.

International Journal of Radiation Oncology*Biology*Physics, 2013

IEEE/ASME Transactions on Mechatronics, 2000

Though the 3-D sensing technique has achieved many successes in various applications, it is still... more Though the 3-D sensing technique has achieved many successes in various applications, it is still a challenge for commercialized sensors to measure the 3-D shape of a transparent, mirror-like reflection-based glass surface. The reason is that the well-known triangle-based structure lighting method is only valid for parts with diffuse reflection property. To measure the 3-D profile of a glass surface, specially an automotive windshield, a new robot-assisted back-imaging system is introduced in this paper. Using the mirror property of the glass, encoded patterns are projected from an LCD monitor and recorded by two cameras. The distortion of structured light patterns, caused by the curvature of the glass, is used to calculate the 3-D shape. Different from the traditional structured light methods, the back-imaging system can simultaneously calculate both the coordinates and normal of the surface, which is particularly required by the glass industry. Furthermore, this paper also introduces a robot planning/control system that integrates the measurement constraints, vision model of the new back-imaging sensor, and the computer-aided design model together to automatically move the back-imaging sensor for 3-D shape measurement. The proposed methods have been implemented, and the sensor is installed on an ABB robot for measuring an automotive side-door glass. Experimental results are reported and analyzed.

Clinical Biochemistry, 1995

Cellular and Molecular Life Sciences, 2006

Reticulons (RTNs) are a group of integral membrane proteins that have a uniquely conserved C-term... more Reticulons (RTNs) are a group of integral membrane proteins that have a uniquely conserved C-terminal domain named RHD. In mammalian genomes, transcripts are produced from four genes, rtn1 to rtn4, under the regulation of tissue or cell-type-specific expression. The presence of alternative promoters for gene expression and multiple cryptic splicing sites have resulted in large numbers of genes/proteins that are classified among the reticulon family. Although this family exists in almost all eukaryotes, only the rtn4 gene product, Nogo (RTN4), has gained relatively more in-depth attention. Despite predominant localization in the endoplasmic reticulum, Nogo on the cell surface appears to play a critical role as an inhibitory molecule for axonal growth and regeneration in humans and rodents. Recently, studies have expanded the biological functions of RTNs to other facets including modulating the enzymatic activity of beta-secretase in Alzheimer's disease. In this review, we summarize the accumulated findings concerning the structural and functional aspects of RTNs and speculate on their linkage to the pathogenesis of neurodegenerative diseases.