Qian Yu - Academia.edu (original) (raw)
Papers by Qian Yu
Journal of hazardous materials, Jan 17, 2015
Environmental impacts of carbon nanotubes (CNTs) arise both from the characteristics of CNTs as w... more Environmental impacts of carbon nanotubes (CNTs) arise both from the characteristics of CNTs as well as from their sorbed contaminants from aquatic environments. In this work, we employed pristine, carboxyl-, hydroxyl- and amino-functionalized multi-walled CNTs and pristine single-walled CNTs to quantify and compare their impacts on aquatic microbial communities in the absence and presence of Cu or Cr. Aliquots of samples were set up to 10 and 40 days for culture-dependent analyses, namely, quantitative real-time polymerase chain reaction and denaturing gradient gel electrophoresis. Results revealed that the presence of CNTs or the mixture of CNTs and metals transiently affected microbial communities, and toxicity of CNTs was enhanced with the addition of metals. Meanwhile, functionalized CNTs exhibited stronger toxicity. The major impacts were observed after 10 days of exposure, but the microbial community could recover at 40 days to some extent. Though microbial communities recovered, total microbial numbers continued to decrease with contact time. Analysis of sequence cloned 16S rDNA indicated that Bacillus sp. and Acidithiobacillus sp. were the dominant taxa. Overall, CNTs would have more serious risk to an ecosystem in the presence of metals.
Science (New York, N.Y.), Jan 6, 2015
Structural alloys are often strengthened through the addition of solute atoms. However, given tha... more Structural alloys are often strengthened through the addition of solute atoms. However, given that solute atoms interact weakly with the elastic fields of screw dislocations, it has long been accepted that solution hardening is only marginally effective in materials with mobile screw dislocations. By using transmission electron microscopy and nanomechanical characterization, we report that the intense hardening effect of dilute oxygen solutes in pure α-Ti is due to the interaction between oxygen and the core of screw dislocations that mainly glide on prismatic planes. First-principles calculations reveal that distortion of the interstitial sites at the screw dislocation core creates a very strong but short-range repulsion for oxygen that is consistent with experimental observations. These results establish a highly effective mechanism for strengthening by interstitial solutes.
ABSTRACT This paper presents a virtual lab of connected vehicle technology. The virtual lab featu... more ABSTRACT This paper presents a virtual lab of connected vehicle technology. The virtual lab features an interactive website where remote users, who are unable to learn connected vehicle technology at expensive real labs, can interact with the virtual environment. A team of traffic engineers, computer scientists/engineers, and electrical engineers propose this integrated and high-fidelity simulation platform to evaluate the timeliness and reliability of network design for vehicle-to-vehicle, vehicle-to-infrastructure, and vehicle-to-handheld device wireless communications. The work pioneers the interdisciplinary research of transportation cyber-physical systems by combining microscopic traffic simulation and computer network simulation. The model incorporates driving maneuvers, traffic dynamics, and control strategies to provide a realistic environment. The influence of transportation features on data rate and channel access of communication network is closely examined to evaluate the timing and reliability requirements. The paper also examines the effectiveness of virtual labs comparable to real labs in engineering education and training.
Guang pu xue yu guang pu fen xi = Guang pu, 2004
In phene-50% NaOH medium, 1,4-dibromobutane reacts with carbazole (CZ) to form N-bromobutylcarbao... more In phene-50% NaOH medium, 1,4-dibromobutane reacts with carbazole (CZ) to form N-bromobutylcarbaole (BCZ) at 70-80 degrees C. The 2-thenoyl-2-carbazolyl-N-butyltrifluoroacetone (TCBTA) was synthesized by reaction of BCZ with 2-thenoyltrifluoroacetone (TTA) in acetone-K2CO3 medium at 50 degrees C. The TCBTA is a light brown crystal with m.p. of 211-214 degrees C. The structure of the synthesized TCBTA was characterized by UV, IR and GC/MS, and the UV and IR spectra of CZ, BCZ and TTA have been studied. In chloroform, TCBTA has three maximum absorptions at 241, 265 and 295 nm whose molar absorptivity is 1.88 x 10(5), 1.25 x 10(5) and 1.08 x 10(5) L x mol(-1) x cm(-1), respectively. Extraction rate of iron(III) and neodymium(III) by TCBTA in chloroform rises by 3 and 1.6 times compared with TTA. The fluorescence of TCBTA-Nd(III) complex is 5 times higher than TTA-Nd(III) complex. It was shown that TCBTA has excellent photosensitivity.
PloS one, 2013
To identify a novel pathogenic gene mutation present in a Chinese family with hereditary hemorrha... more To identify a novel pathogenic gene mutation present in a Chinese family with hereditary hemorrhagic telangiectasia (HHT) and to determine if an intron mutation may influence the transcriptional activity of the ACVRL1 gene. HHT family members were ascertained following the presentation of proband and involved subjects. All family members (n = 5) and 113 healthy individuals were genotyped for the variant in intron 6 c.772+27G>C of ACVRL1 gene. The genomic structure of ACVRL1 in affected HHT patients and healthy individuals was determined by long range PCR and sequencing. The expression of ACVRL1 mRNA and protein in patients with HHT was evaluated using real-time polymerase chain reaction and immunoblot analysis. Luciferase activity assay and electrophoretic mobility shift assay (EMSA) were performed to uncover the mechanism of intron-related transcriptional regulation. Only one novel mutation in intron 6 (c.772+27G>C) of ACVRL1 gene, no other mutation, abnormal splice, gross genomic deletion or rearrangement was found in this HHT2 family. Compared with healthy individuals, ACVRL1 mRNA and protein were significantly decreased in affected HHT2 individuals. Luciferase activity assay demonstrated that the transcriptional activity of the mutated ACVRL1 was significantly lower than that of the wild-type of intron 6; EMSA results showed that intron 6 c.772+27G>C mutation was able to inhibit the binding of transcriptional factor Sp1. A novel intron mutation in ACVRL1 gene is associated with familial HHT2. The mechanisms may be involved in the down-regulation of ACVRL1 gene transcription.
Phytochemical analysis : PCA
Veterinary parasitology, Jan 29, 2014
Veterinary parasitology, Jan 15, 2014
The rhoptry-associated protein 1 (RAP-1) gene of Babesia orientalis was obtained from a cDNA expr... more The rhoptry-associated protein 1 (RAP-1) gene of Babesia orientalis was obtained from a cDNA expression library by immunoscreening with B. orientalis-infected water buffalo sera. The nucleotide sequence of the cDNA was 1732 bp with an open reading frame (ORF) of 1434 bp, encoding a polypeptide of 478 amino acid residues with a predicted size of 52.5 kDa. The ORF was cloned into a pGEX-KG plasmid and subsequently expressed as a GST-fusion protein. The recombinant RAP-1 of B. orientalis (rBoRAP-1) was purified and evaluated as an antigen using Western blotting. The native BoRAP-1 was recognized by the antibodies raised in rabbits against rBoRAP-1. Strong immunofluorescence signals were observed in erythrocytes infected with B. orientalis. Phylogentic analysis revealed that B. orientalis fell into a Babesia clade and most closely related to Babesia bovis and Babesia ovis, which was similar to the previous reported trees based on 18S rRNA and HSP70 genes. The present study suggests that the BoRAP-1 might be a potential diagnostic antigen, and the RAP-1 genes can aid in the classification of Babesia and Theileria species.
Phytotherapy research : PTR, Jan 3, 2014
Phytochemical investigation of methanol extract from the spikes of Prunella vulgaris L. led to th... more Phytochemical investigation of methanol extract from the spikes of Prunella vulgaris L. led to the isolation of two new pentacyclic triterpenoid glycosides Vulgasides I (1) and II (2) along with 13 known compounds (3-15). Their structures were established on the basis of nuclear magnetic resonance (1D and 2D) and mass spectroscopic data analysis. All the isolated compounds were screened for glycogen phosphorylase inhibitory activity and also evaluated for their effect on insulin sensitivity in 3T3-L1 adipocytes. Two new compounds (1, 2) did not demonstrate the glycogen phosphorylase inhibitory activity, but other compounds (3-11) exhibited varying degrees of glycogen phosphorylase inhibitory activity with IC50 values in the range from 30.69 to 68.85 μM. Compounds 3, 6, 7, 11, and 13 demonstrated markedly increased insulin-mediated glucose consumption in 3T3-L1 adipocytes. Copyright © 2014 John Wiley & Sons, Ltd.
Journal of hazardous materials, Jan 17, 2015
Environmental impacts of carbon nanotubes (CNTs) arise both from the characteristics of CNTs as w... more Environmental impacts of carbon nanotubes (CNTs) arise both from the characteristics of CNTs as well as from their sorbed contaminants from aquatic environments. In this work, we employed pristine, carboxyl-, hydroxyl- and amino-functionalized multi-walled CNTs and pristine single-walled CNTs to quantify and compare their impacts on aquatic microbial communities in the absence and presence of Cu or Cr. Aliquots of samples were set up to 10 and 40 days for culture-dependent analyses, namely, quantitative real-time polymerase chain reaction and denaturing gradient gel electrophoresis. Results revealed that the presence of CNTs or the mixture of CNTs and metals transiently affected microbial communities, and toxicity of CNTs was enhanced with the addition of metals. Meanwhile, functionalized CNTs exhibited stronger toxicity. The major impacts were observed after 10 days of exposure, but the microbial community could recover at 40 days to some extent. Though microbial communities recovered, total microbial numbers continued to decrease with contact time. Analysis of sequence cloned 16S rDNA indicated that Bacillus sp. and Acidithiobacillus sp. were the dominant taxa. Overall, CNTs would have more serious risk to an ecosystem in the presence of metals.
Science (New York, N.Y.), Jan 6, 2015
Structural alloys are often strengthened through the addition of solute atoms. However, given tha... more Structural alloys are often strengthened through the addition of solute atoms. However, given that solute atoms interact weakly with the elastic fields of screw dislocations, it has long been accepted that solution hardening is only marginally effective in materials with mobile screw dislocations. By using transmission electron microscopy and nanomechanical characterization, we report that the intense hardening effect of dilute oxygen solutes in pure α-Ti is due to the interaction between oxygen and the core of screw dislocations that mainly glide on prismatic planes. First-principles calculations reveal that distortion of the interstitial sites at the screw dislocation core creates a very strong but short-range repulsion for oxygen that is consistent with experimental observations. These results establish a highly effective mechanism for strengthening by interstitial solutes.
ABSTRACT This paper presents a virtual lab of connected vehicle technology. The virtual lab featu... more ABSTRACT This paper presents a virtual lab of connected vehicle technology. The virtual lab features an interactive website where remote users, who are unable to learn connected vehicle technology at expensive real labs, can interact with the virtual environment. A team of traffic engineers, computer scientists/engineers, and electrical engineers propose this integrated and high-fidelity simulation platform to evaluate the timeliness and reliability of network design for vehicle-to-vehicle, vehicle-to-infrastructure, and vehicle-to-handheld device wireless communications. The work pioneers the interdisciplinary research of transportation cyber-physical systems by combining microscopic traffic simulation and computer network simulation. The model incorporates driving maneuvers, traffic dynamics, and control strategies to provide a realistic environment. The influence of transportation features on data rate and channel access of communication network is closely examined to evaluate the timing and reliability requirements. The paper also examines the effectiveness of virtual labs comparable to real labs in engineering education and training.
Guang pu xue yu guang pu fen xi = Guang pu, 2004
In phene-50% NaOH medium, 1,4-dibromobutane reacts with carbazole (CZ) to form N-bromobutylcarbao... more In phene-50% NaOH medium, 1,4-dibromobutane reacts with carbazole (CZ) to form N-bromobutylcarbaole (BCZ) at 70-80 degrees C. The 2-thenoyl-2-carbazolyl-N-butyltrifluoroacetone (TCBTA) was synthesized by reaction of BCZ with 2-thenoyltrifluoroacetone (TTA) in acetone-K2CO3 medium at 50 degrees C. The TCBTA is a light brown crystal with m.p. of 211-214 degrees C. The structure of the synthesized TCBTA was characterized by UV, IR and GC/MS, and the UV and IR spectra of CZ, BCZ and TTA have been studied. In chloroform, TCBTA has three maximum absorptions at 241, 265 and 295 nm whose molar absorptivity is 1.88 x 10(5), 1.25 x 10(5) and 1.08 x 10(5) L x mol(-1) x cm(-1), respectively. Extraction rate of iron(III) and neodymium(III) by TCBTA in chloroform rises by 3 and 1.6 times compared with TTA. The fluorescence of TCBTA-Nd(III) complex is 5 times higher than TTA-Nd(III) complex. It was shown that TCBTA has excellent photosensitivity.
PloS one, 2013
To identify a novel pathogenic gene mutation present in a Chinese family with hereditary hemorrha... more To identify a novel pathogenic gene mutation present in a Chinese family with hereditary hemorrhagic telangiectasia (HHT) and to determine if an intron mutation may influence the transcriptional activity of the ACVRL1 gene. HHT family members were ascertained following the presentation of proband and involved subjects. All family members (n = 5) and 113 healthy individuals were genotyped for the variant in intron 6 c.772+27G>C of ACVRL1 gene. The genomic structure of ACVRL1 in affected HHT patients and healthy individuals was determined by long range PCR and sequencing. The expression of ACVRL1 mRNA and protein in patients with HHT was evaluated using real-time polymerase chain reaction and immunoblot analysis. Luciferase activity assay and electrophoretic mobility shift assay (EMSA) were performed to uncover the mechanism of intron-related transcriptional regulation. Only one novel mutation in intron 6 (c.772+27G>C) of ACVRL1 gene, no other mutation, abnormal splice, gross genomic deletion or rearrangement was found in this HHT2 family. Compared with healthy individuals, ACVRL1 mRNA and protein were significantly decreased in affected HHT2 individuals. Luciferase activity assay demonstrated that the transcriptional activity of the mutated ACVRL1 was significantly lower than that of the wild-type of intron 6; EMSA results showed that intron 6 c.772+27G>C mutation was able to inhibit the binding of transcriptional factor Sp1. A novel intron mutation in ACVRL1 gene is associated with familial HHT2. The mechanisms may be involved in the down-regulation of ACVRL1 gene transcription.
Phytochemical analysis : PCA
Veterinary parasitology, Jan 29, 2014
Veterinary parasitology, Jan 15, 2014
The rhoptry-associated protein 1 (RAP-1) gene of Babesia orientalis was obtained from a cDNA expr... more The rhoptry-associated protein 1 (RAP-1) gene of Babesia orientalis was obtained from a cDNA expression library by immunoscreening with B. orientalis-infected water buffalo sera. The nucleotide sequence of the cDNA was 1732 bp with an open reading frame (ORF) of 1434 bp, encoding a polypeptide of 478 amino acid residues with a predicted size of 52.5 kDa. The ORF was cloned into a pGEX-KG plasmid and subsequently expressed as a GST-fusion protein. The recombinant RAP-1 of B. orientalis (rBoRAP-1) was purified and evaluated as an antigen using Western blotting. The native BoRAP-1 was recognized by the antibodies raised in rabbits against rBoRAP-1. Strong immunofluorescence signals were observed in erythrocytes infected with B. orientalis. Phylogentic analysis revealed that B. orientalis fell into a Babesia clade and most closely related to Babesia bovis and Babesia ovis, which was similar to the previous reported trees based on 18S rRNA and HSP70 genes. The present study suggests that the BoRAP-1 might be a potential diagnostic antigen, and the RAP-1 genes can aid in the classification of Babesia and Theileria species.
Phytotherapy research : PTR, Jan 3, 2014
Phytochemical investigation of methanol extract from the spikes of Prunella vulgaris L. led to th... more Phytochemical investigation of methanol extract from the spikes of Prunella vulgaris L. led to the isolation of two new pentacyclic triterpenoid glycosides Vulgasides I (1) and II (2) along with 13 known compounds (3-15). Their structures were established on the basis of nuclear magnetic resonance (1D and 2D) and mass spectroscopic data analysis. All the isolated compounds were screened for glycogen phosphorylase inhibitory activity and also evaluated for their effect on insulin sensitivity in 3T3-L1 adipocytes. Two new compounds (1, 2) did not demonstrate the glycogen phosphorylase inhibitory activity, but other compounds (3-11) exhibited varying degrees of glycogen phosphorylase inhibitory activity with IC50 values in the range from 30.69 to 68.85 μM. Compounds 3, 6, 7, 11, and 13 demonstrated markedly increased insulin-mediated glucose consumption in 3T3-L1 adipocytes. Copyright © 2014 John Wiley & Sons, Ltd.